施氏鲟性别相关的微卫星标记筛选
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摘要
用32个微卫星标记分析了30尾(15尾♀;15尾♂)性成熟(12龄)施氏鲟(Acipenser schrenckii)雌雄个体的遗传差异。结果表明:雌、雄群体的遗传多样性差异没有达到显著水平。在6个微卫星标记中有9个等位基因在雌、雄群体中分布比例差异达到50%以上,其中雌性群体HLJSX194-239bp、HLJSX201-207bp、HLJSX209-210bp、HLJSX210-178bp和HLJSX215-221bp5个等位基因频率显著高于雄性群体;雄性群体HLJSX194-184bp、HLJSX201-214bp、HLJSX210-233bp和HLJSX226-194bp 4个等位基因频率显著高于雌性群体,初步表明这些基因位点可能与性别决定位点存在一定连锁关系。本研究结果为施氏鲟早期性别鉴定积累了基础数据。
In this paper, 33 microsatellite markers were used to screen the sex-related DNA markers through analyzing DNA differences between female and male in 12 years old mature Amur sturgeon(Acipenser schrenckii)(15 samples).The results showed that there were no significant differences between the female and the male populations. However, 9 alleles derived from 6 SSR loci showed different distributions between female and male groups at a rate of >50%. Five alleles HLJSX194-239bp,HLJSX201-207bp, HLJSX209-210bp, HLJSX210 178 bp, and HLJSX215-221bp were found to be significantly higher frequency in the female samples than those in the male samples. However, there was significantly higher frequency in 4 alleles HLJSX194-184bp, HLJSX201-214bp,HLJSX210-233bp, and HLJSX226-194bp in the male samples than those in the female samples, indicating that these loci are linked with sex-determinate loci. The findings provide a basis for gender identification during early development of Amur sturgeon.
In this paper, 33 microsatellite markers were used to screen the sex-related DNA markers through analyzing DNA differences between female and male in 12 years old mature Amur sturgeon(Acipenser schrenckii)(15 samples).The results showed that there were no significant differences between the female and the male populations. However, 9 alleles derived from 6 SSR loci showed different distributions between female and male groups at a rate of >50%. Five alleles HLJSX194-239bp,HLJSX201-207bp, HLJSX209-210bp, HLJSX210 178 bp, and HLJSX215-221bp were found to be significantly higher frequency in the female samples than those in the male samples. However, there was significantly higher frequency in 4 alleles HLJSX194-184bp, HLJSX201-214bp,HLJSX210-233bp, and HLJSX226-194bp in the male samples than those in the female samples, indicating that these loci are linked with sex-determinate loci. The findings provide a basis for gender identification during early development of Amur sturgeon.
引文
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[24]张涛,章龙珍,赵峰,等.西伯利亚鲟不同性别与卵巢发育时期血液性类固醇激素的差异与判别分析[J].海洋渔业,2008,30(1):19-25.
[25]邱晴,罗文.采用RAPD技术筛选马口鱼性别差异相关分子标记[J].水产养殖,2012,33(1):39-42.
[26]张庭,卢迈新,叶星,等.奥里亚罗非鱼微卫星位点的筛选[J].广东海洋大学学报,2007,27(3):6-10.
[27]宋苏祥,刘洪柏,孙大江,等.施氏鲟的核型及DNA含量研究[J].遗传,1997,19(3):5-8.
[2]孙大江,曲秋芝,张颖,等.中国的鲟鱼养殖[J].水产学杂志,2011,24(4):67-70.
[3]孙大江,张颖,马国军.鲟鱼子酱的生产与国际贸易概况.[J].水产学杂志,2014,27(1):1-7.
[4]赵峰,章龙珍,庄平,等.鲟科鱼类性别鉴别技术的研究进展及其应用[J].海洋渔业,2009,31(2):215-220.
[5]张颖,孙大江,曲秋芝,等.不同温度下雌激素对施氏鲟早期性腺发育及血清性激素的影响[J].东北农业大学学报,2011,42(12):118-124.
[6]章龙珍,庄平,张涛,等.人工养殖施氏鲟性腺发育的观察[J].中国水产科学,2002,9(4):321-327.
[7]Mobery G P,Watson J G,Doroshov S,et al.Physiological evidence for two sturgeon gonadotropins in Acipenser transmontanus[J].Aqu,1995,135(1):27-39.
[8]Williot P,Brun R,Rouault T,et al.Management of female spawners of the Siberian sturgeon Acipenser baeri:first results[A].In:Acipenser.Actes du Colloque[C].Bordeaus:CEMAGREF,1991:365-380.
[9]O’Connell M and Wright J M.Microsatellite DNA in fishes[J].Rev Fish Biol Fish,1997,7:331-363.
[10]孙效文,张晓锋,赵莹莹,等.水产生物微卫星标记技术研究进展及其应用[J].中国水产科学,2008,15(4):689-703.
[11]萨姆布鲁克,拉塞尔D W.分子克隆实验指南[M].北京:科学出版社,2002:8.
[12]Liu Y,Li C,Cheng L,et al.Development and characterization of new microsatellite markers for Amur sturgeon(Acipenser schrenckii)[J].Concervation Genet Resour.2013,Doi:10.1007/s12686-013-0006-6.
[13]刘翠.施氏鲟微卫星克隆及其性别相关AFLP标记的筛选[D].哈尔滨:哈尔滨师范大学,2011.
[14]毛建春,任波.施氏鲟全人工饲料苗种培育试验[J].黑龙江水产,2010(2):1-3.
[15]畅雅萍,徐奇友,孙大江,等.几种诱食剂对施氏鲟(Acipenser schrenckii)生长性能、体成分和血液生化指标的影响[J].水产学杂志,2009,22(3):23-27.
[16]孙大江,曲秋芝,马国军,等.史氏鲟人工养殖研究现状与展望[J].水产学杂志,1998,5(3):108-111.
[17]Li C,Lu C Y,Li J T,et al.Complete mitochondrial genome of Amur sturgeon(Acipenser schrenckii)[J].Mitochondrial DNA,2013,DOI:10.3109/19401736.2013.800507.
[18]Botstein D,White R L,Skolnick M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorpisms[J].Am J Human Genet,1980,32:314-331.
[19]Liao X L,Ma H Y,Xu G B,et al.Construction of a genetic linkage map and mapping of a female specific DNA marker in half-smooth tongue sole(Cynoglossus semilaevis)[J].Mar Biotechnol,2009,11:699-709.
[20]Coimbra M R M,Kobayashi K,Koretsugu S,et al.A genetic linkage map of the Japanese flounder,Paralichthys olivaceus[J].Aquaculture,2003,220(1):203-218.
[21]Waldbieser G C,Bosworth B G,Nonneman D J,et al.A microsatellite-based genetic linkagemap for channel catfish,Ictuluras punctatus[J].Genetics,2001,158(2):727-734.
[22]Sakamoto T,Danzmann R G,Gharbi K,et al.A microsatellite linkage map of rainbow trout(Oncorhynchus mykiss)characterized by large sex-specific differences in recombination rates[J].Genetics,2000,155:1331-1345.
[23]Woram R A,Gharbi K,Sakamota T,et al.Comparative genomeanalysis of the primary sex determining locus in salmonid fishes[J].Genome Res,2003,1(3):272-280.
[24]张涛,章龙珍,赵峰,等.西伯利亚鲟不同性别与卵巢发育时期血液性类固醇激素的差异与判别分析[J].海洋渔业,2008,30(1):19-25.
[25]邱晴,罗文.采用RAPD技术筛选马口鱼性别差异相关分子标记[J].水产养殖,2012,33(1):39-42.
[26]张庭,卢迈新,叶星,等.奥里亚罗非鱼微卫星位点的筛选[J].广东海洋大学学报,2007,27(3):6-10.
[27]宋苏祥,刘洪柏,孙大江,等.施氏鲟的核型及DNA含量研究[J].遗传,1997,19(3):5-8.