玉米须总皂苷对肥胖大鼠脂肪组织的调节作用研究
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摘要
目的观察玉米须总皂苷对高脂饮食诱导的肥胖大鼠脂肪组织中G0/G1期开关基因(G0S2)和脂肪甘油三酯脂酶(ATGL)表达的影响。方法将40只适应性喂养7 d后雄性清洁级SD大鼠随机分为正常组、模型组、玉米须总皂苷低剂量和玉米须总皂苷高剂量组,每组10只。除正常组外,其余3组均给予高脂高糖饮食12周建立肥胖大鼠模型。造模成功后均给予普通饲料喂养,同时玉米须总皂苷低剂量组和高剂量组分别给予玉米须总皂苷100 mg/(kg·d)和200 mg/(kg·d)灌胃,正常组和模型组给予生理盐水1m L/(kg·d)灌胃,均1次/d,连续12周。12周后腹主动脉取血,检测各组大鼠空腹血糖(FPG)和血脂指标[三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)]、血清ATGL以及空腹胰岛素(FINS)水平,计算大鼠体脂比,HE染色观察脂肪组织病理学变化,RT-PCR法和Western Blot法检测脂肪细胞中G0S2和ATGL mRNA及蛋白表达水平。结果模型组大鼠体质量、内脏脂肪湿质量、体脂比及FPG、TG、TC、LDL-C、FINS水平均明显高于正常组(P均<0. 05),而玉米须总皂苷低剂量组和玉米须总皂苷高剂量组均明显低于模型组(P均<0. 05);模型组大鼠HDL-C、ATGL水平均明显低于正常组(P均<0. 05),而玉米须总皂苷低剂量组和玉米须总皂苷高剂量组均明显高于模型组(P均<0. 05)。HE染色显示玉米须总皂苷低剂量组和玉米须总皂苷高剂量组脂肪细胞体积明显减小,数量明显增多。模型组大鼠脂肪组织中G0S2mRNA和G0S2蛋白表达水平均明显高于正常组(P均<0. 05),ATGL mRNA和ATGL蛋白表达水平均明显低于正常组(P均<0. 05);玉米须总皂苷低剂量组和玉米须总皂苷高剂量组G0S2 mRNA和G0S2蛋白表达水平均明显低于模型组(P均<0. 05),而ATGL mRNA和ATGL蛋白表达水平均明显高于模型组(P均<0. 05),玉米须总皂苷低剂量组和玉米须总皂苷高剂量组组间比较差异均无统计学意义(P均> 0. 05)。结论玉米须总皂苷能够明显下调脂肪组织中G0S2 mRNA和蛋白表达、上调ATGL mRNA和蛋白表达,进而促进肥胖大鼠脂肪分解,降低大鼠体质量。
Objective It is to observe the effects of total saponin of corn stigma on G0/G1 phase switch genes(G0S2)and adipose triglyceride lipase(ATGL) in adipose tissue of obese rats induced by high fat diet. Methods Forty male SD rats after adaptive feeding for 7 days were randomly divided into normal group,model group,low dose of total saponin of corn stigma group,and high dose of total saponin of corn stigma group,10 rats in each group. Except the rats in the normal group,the ones in the other three groups were given a high-fat and high-sugar diet for 12 weeks to establish obese rat models. After successful modeling,the common feed was given. At the same time,the low-dose and high-dose groups of corn stigma were given total saponins of corn stigma 100 mg/(kg·d) and 200 mg/(kg·d) respectively by lavage. The rats in normal group and model group were given normal saline 1 m L/(kg · d),all 1 time/d,treating for 12 weeks. After 12 weeks,blood was taken from the abdominal aorta to determine the levels of fasting blood glucose(FPG) and blood lipids[triacylglycerol(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C) ],serum ATGL and fasting insulin(FINS) in each group,body fat ratio of the rats was calculated. HE staining was used to observe the pathological changes of adipose tissue. RT-PCR and Western Blot were used to detect the expression levels of G0S2 and ATGL mRNA in adipocytes. Results The body weight,visceral fat mass,body fat ratio and FPG,TG,TC,LDL-C and FINS levels in the model group were significantly higher than those in the normal group(P <0. 05),while these indexes were significantly lower in low dose of total saponin of corn stigma group and high dose of total saponin of corn stigma group than the model group(P < 0. 05). The HDL-C and ATGL levels in the model group were significantly lower than those in the normal group(P < 0. 05),while these indexes were significantly higher in low dose of total saponin of corn stigma group and high dose of total saponin of corn stigma group than the model group(P < 0. 05). HE staining showed that the volume of adipocytes decreased significantly,and the number increased significantly in the low dose of total saponin of corn stigma group and high dose of total saponin of corn stigma group. The expression levels of G0S2 mRNA and G0S2 protein in the adipose tissue of the model group were significantly higher while the levels of ATGL mRNA and ATGL protein were significantly lower than those in the normal group(P < 0. 05). The expression levels of G0S2 mRNA and G0S2 protein in the low-dose saponin group and the high-dose saponin group were significantly lower than those in the model group(P < 0. 05),while the expression levels of ATGL mRNA and ATGL protein were significantly higher than those in the model group(P < 0. 05),there was no significant difference between the low-dose group and the high-dose group of corn saponins(P > 0. 05). Conclusion Total saponins of corn stigma can significantly down-regulate the expression of G0S2 mRNA and protein in adipose tissue,up-regulate the expression of ATGL mRNA and protein,and promote the decomposition of fat in obese rats and reduce the body mass of rats.
Objective It is to observe the effects of total saponin of corn stigma on G0/G1 phase switch genes(G0S2)and adipose triglyceride lipase(ATGL) in adipose tissue of obese rats induced by high fat diet. Methods Forty male SD rats after adaptive feeding for 7 days were randomly divided into normal group,model group,low dose of total saponin of corn stigma group,and high dose of total saponin of corn stigma group,10 rats in each group. Except the rats in the normal group,the ones in the other three groups were given a high-fat and high-sugar diet for 12 weeks to establish obese rat models. After successful modeling,the common feed was given. At the same time,the low-dose and high-dose groups of corn stigma were given total saponins of corn stigma 100 mg/(kg·d) and 200 mg/(kg·d) respectively by lavage. The rats in normal group and model group were given normal saline 1 m L/(kg · d),all 1 time/d,treating for 12 weeks. After 12 weeks,blood was taken from the abdominal aorta to determine the levels of fasting blood glucose(FPG) and blood lipids[triacylglycerol(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C) ],serum ATGL and fasting insulin(FINS) in each group,body fat ratio of the rats was calculated. HE staining was used to observe the pathological changes of adipose tissue. RT-PCR and Western Blot were used to detect the expression levels of G0S2 and ATGL mRNA in adipocytes. Results The body weight,visceral fat mass,body fat ratio and FPG,TG,TC,LDL-C and FINS levels in the model group were significantly higher than those in the normal group(P <0. 05),while these indexes were significantly lower in low dose of total saponin of corn stigma group and high dose of total saponin of corn stigma group than the model group(P < 0. 05). The HDL-C and ATGL levels in the model group were significantly lower than those in the normal group(P < 0. 05),while these indexes were significantly higher in low dose of total saponin of corn stigma group and high dose of total saponin of corn stigma group than the model group(P < 0. 05). HE staining showed that the volume of adipocytes decreased significantly,and the number increased significantly in the low dose of total saponin of corn stigma group and high dose of total saponin of corn stigma group. The expression levels of G0S2 mRNA and G0S2 protein in the adipose tissue of the model group were significantly higher while the levels of ATGL mRNA and ATGL protein were significantly lower than those in the normal group(P < 0. 05). The expression levels of G0S2 mRNA and G0S2 protein in the low-dose saponin group and the high-dose saponin group were significantly lower than those in the model group(P < 0. 05),while the expression levels of ATGL mRNA and ATGL protein were significantly higher than those in the model group(P < 0. 05),there was no significant difference between the low-dose group and the high-dose group of corn saponins(P > 0. 05). Conclusion Total saponins of corn stigma can significantly down-regulate the expression of G0S2 mRNA and protein in adipose tissue,up-regulate the expression of ATGL mRNA and protein,and promote the decomposition of fat in obese rats and reduce the body mass of rats.
引文
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[17]Turnbull PC,Ramos SV,Macpherson RE,et al. Characterization of lipolytic inhibitor G(0)/G(1)switch gene-2protein(G0S2)expression in male Sprague-Dawley rat skeletal muscle compared to relative content of adipose triglyceride lipase(ATGL)and comparitive gene identification-58(CGI-58)[J]. PLo S One,2015,10(3):e0120136
[18]王存川,张艺超.肥胖症与代谢疾病外科治疗的发展[J].中华消化外科杂志,2015,14(7):524-527
[19]涂长春,李晓宇,涂翌利,等.宁红瘦身含片对肥胖高脂血症模型大鼠作用研究[J].现代中西医结合杂志,2003,12(1):11-12
[20]龚美蓉,陈凤丽,曹晨,等.针刺通过AMPK信号通路改善肥胖大鼠瘦素抵抗的机制[J].现代中西医结合杂志,2013,22(22):2500-2501
[2]张艺宏,王梅,孙君志,等. 2014年中国城乡居民超重肥胖流行现状--基于22省(市、区)国家国民体质监测点的形态数据[J].成都体育学院学报,2016,42(5):93-100
[3]陈晓梅.体质量指数与2型糖尿病患者心血管危险因素的相关性分析[J].现代中西医结合杂志,2011,20(10):1217
[4]张颖,张晓丹.肥胖的发病机制及其中药治疗概况[J].中医药信息,2007,24(5):13-15
[5]张彬.中成药治疗高脂血症用药规律研究[J].中国实用医药,2017,12(17):194-195
[6]陈翰卿,王旭.玉米须治疗糖尿病相关研究进展[J].浙江中医药大学学报,2012,36(1):104-105
[7]余渊,程杰.玉米须总皂苷对非酒精性脂肪肝病大鼠肝脏PPARγ表达的影响[J].长春中医药大学学报,2017,33(4):530-533
[8]程彦臻,陈宏,杨帆,等.广东省超重、肥胖2型糖尿病患者慢性并发症及合并症发病情况调查[J].山东医药,2016,56(31):52-54
[9]王兴纯,曲伸.腹型肥胖及其危害[J].糖尿病临床,2015,9(3):135-136
[10] Jaeger D,Schoiswohl G,Hofer P,et al. Fasting-induced G0/G1 switch gene 2 and FGF21 expression in the liver are under regulation of adipose tissue derived fatty acids[J]. J Hepatol,2015,63(2):437
[11] Skopp A,May M,Janke J,et al. Regulation of G0/G1switch gene 2(G0S2)expression in human adipose tissue[J]. Arch Physiol Biochem,2016,122(2):47-53
[12] Claire L,Pierre-Marie B,Katie L,et al. G0/G1 Switch Gene 2 controls adipose triglyceride lipase activity and lipid metabolism in skeletal muscle[J]. Mol Metab,2016,5(7):527-537
[13] Zimmerman TP,Potischman N,Douglass D,et al. The Effect of Editing Open-Ended Text Responses on Nutrient and Food Group Estimates from the Automated Self-Administered 24-Hour Dietary Recall(ASA24)[J]. Procedia Food Science,2015,4(1):160-172
[14]Khan SA,Sathyanarayan A,Mashek MT,et al. ATGL-Catalyzed Lipolysis Regulates SIRT1 to Control PGC-1α/PPAR-αSignaling[J]. Diabetes,2015,64(2):418-426
[15]Fischer J,Lefèvre C,Morava E,et al. The gene encoding adipose triglyceride lipase(PNPLA2)is mutated in neutral lipid storage disease with myopathy[J]. Nat Genet,2007,39(1):28-30
[16]Heckmann BL,Zhang X,Saarinen AM,et al. Regulation of G0/G1 Switch Gene 2(G0S2)Protein Ubiquitination and Stability by Triglyceride Accumulation and ATGL Interaction[J]. PLo S One,2016,11(6):e0156742
[17]Turnbull PC,Ramos SV,Macpherson RE,et al. Characterization of lipolytic inhibitor G(0)/G(1)switch gene-2protein(G0S2)expression in male Sprague-Dawley rat skeletal muscle compared to relative content of adipose triglyceride lipase(ATGL)and comparitive gene identification-58(CGI-58)[J]. PLo S One,2015,10(3):e0120136
[18]王存川,张艺超.肥胖症与代谢疾病外科治疗的发展[J].中华消化外科杂志,2015,14(7):524-527
[19]涂长春,李晓宇,涂翌利,等.宁红瘦身含片对肥胖高脂血症模型大鼠作用研究[J].现代中西医结合杂志,2003,12(1):11-12
[20]龚美蓉,陈凤丽,曹晨,等.针刺通过AMPK信号通路改善肥胖大鼠瘦素抵抗的机制[J].现代中西医结合杂志,2013,22(22):2500-2501