超高效液相色谱质谱联用测定咸鱼中16种有机磷农药残留
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摘要
本文建立了咸鱼中16种有机磷农药残留的超高效液相色谱串联三重四级杆质谱分析方法,线性范围在0.5～50ng/mL,线性相关系数均分布在0.991～0.999。本文前处理方法采用的是QuEChERS方法,该方法回收率在75.45%～100.37%,结果表明,在处理咸鱼含有较多脂肪、蛋白质等复杂基质时,QuEChERS方法具有操作简便快速、准确率高、重复性好等优点,超高效液相色谱串联三重四级杆质谱的仪器方法与气相色谱法相比,具有灵敏度和准确度高的优点。
This paper established an ultra performance liquid chromatography tandem triple quadrupole mass spectrometry method for the determination of 16 organophosphorus pesticide residues in salted fish. The linear range is between 0.5 ～50 ng/mL, and the linear correlation coefficient is between 0.991 ～ 0.999. The pretreatment method used in this paper was the pretreatment of salted fish by QuEChERS pretreatment method. The recovery rate of QuEChERS method was between 75.45% ～ 100.37%. The results showed that the QuEChERS method had a complex matrix containing more fat and protein. The operation is simple and fast, the accuracy is high, and the repeatability is good. The instrumentation method of ultrahigh performance liquid chromatography tandem triple quadrupole mass spectrometry has the advantages of high sensitivity and reliable accuracy compared with gas chromatography.
This paper established an ultra performance liquid chromatography tandem triple quadrupole mass spectrometry method for the determination of 16 organophosphorus pesticide residues in salted fish. The linear range is between 0.5 ～50 ng/mL, and the linear correlation coefficient is between 0.991 ～ 0.999. The pretreatment method used in this paper was the pretreatment of salted fish by QuEChERS pretreatment method. The recovery rate of QuEChERS method was between 75.45% ～ 100.37%. The results showed that the QuEChERS method had a complex matrix containing more fat and protein. The operation is simple and fast, the accuracy is high, and the repeatability is good. The instrumentation method of ultrahigh performance liquid chromatography tandem triple quadrupole mass spectrometry has the advantages of high sensitivity and reliable accuracy compared with gas chromatography.
引文
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[11]林涛,邵金良,刘兴勇,等.QuEChERS-超高效液相色谱-串联质谱法测定蔬菜中41种农药残留[J].色谱,2015(3):235-241.
[12]刘国栋.微波消解-原子吸收-原子荧光法快速测定粮食中铅、镉、汞、砷[J].粮食科技与经济,2017(6):46-48.
[2]唐黎标.蔬菜农药残留标准现状分析[J].山东农药信息,2019(1):17-18.
[3]刘丽丽,刘巍.食品中农药残留检测技术的研究[J].科学技术创新,2019(2):33-34.
[4]曹俊杰,于霞,胡畔.探讨QuEChERS方法在食品农兽药残留检测中的应用[J].现代食品,2018(23):83-85+89.
[5]易江华,段振娟,方国臻,等.QuEChERS方法在食品农兽药残留检测中的应用[J].中国食品学报,2013(2):153-158.
[6]刘婷.QuEChERS结合GC-MS/MS和LC-MS/MS高通量检测果蔬农药多残留研究[D].长沙:中南林业科技大学,2018.
[7]王耀,刘少彬,谢翠美,等.加速溶剂萃取凝胶渗透色谱/固相萃取净化气相色谱质谱法测定咸鱼中有机磷农药残留[J].分析化学,2011(1):67-71.
[8]彭喜春,赖毅东.咸鱼制品中有机磷农药残留的毛细管GC-NPD法测定[J].食品科学,2006(9):226-228.
[9]吴南村,刘春华,乐渊,等.Qu ECh ERS-超高压液相色谱-串联质谱法检测黑胡椒中6种农药残留[J].分析试验室,2018(12):1431-1435.
[10]刘美辰,路宏博,赵小旭,等.胶体金免疫层析法快速定量检测糙米中重金属离子铅[J].粮食科技与经济,2018(2):61-64.
[11]林涛,邵金良,刘兴勇,等.QuEChERS-超高效液相色谱-串联质谱法测定蔬菜中41种农药残留[J].色谱,2015(3):235-241.
[12]刘国栋.微波消解-原子吸收-原子荧光法快速测定粮食中铅、镉、汞、砷[J].粮食科技与经济,2017(6):46-48.