吡虫啉悬浮种衣剂对麦无网长管蚜实验种群的影响
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摘要
利用吡虫啉种子包衣技术防治麦蚜是我国小麦主产区一项重要的防控措施,目前对麦蚜的防控效果评价主要集中在自然混合种群上,对室内种群尤其是麦无网长管蚜的研究报道较少。为了明确吡虫啉种衣剂对单一蚜虫种类的影响,本文利用吡虫啉悬浮种衣剂处理的小麦苗接种4种蚜虫,分别于3、6和9 d后检查死亡率。并采用生命表方法评价种衣剂处理对麦无网长管生长发育、繁殖和种群生命参数的影响。结果表明:种衣剂处理小麦苗接种的4种蚜虫,3 d后死亡率大小依次为禾谷缢管蚜>麦长管蚜>麦二叉蚜>麦无网长管蚜,6 d后禾谷缢管蚜和麦长管蚜全部死亡;9 d后麦二叉蚜全部死亡,但麦无网长管蚜死亡率仅为10.3%。生命表结果表明:麦无网长管蚜大部分个体在种衣剂处理麦苗上可以完成整个世代周期,但其生长发育延缓,存活率、寿命和繁殖力显著下降,内禀增长率(r_m)、周限增长率(λ)、净生殖率(R_0)显著降低,平均世代周期(T)显著延长。研究结果进一步证实吡虫啉包衣处理对麦长管蚜、禾谷缢管蚜、麦二叉蚜具有很好的防治效果,但对麦无网长管蚜防效较差。
Imidacloprid seed coating treatment has been suggested as an important management for wheat aphids in the major grain-producing areas in China. The effect of imidacloprid on the control of aphids was mainly evaluated in mixed natural populations, but few studies were conducted in the laboratory population, especially Metopolophium dirhodum. To determine the effect of imidacloprid seed coating on grain aphids, four aphid species were inoculated on wheat plants seed-treated with imidacloprid, and the aphids mortality was calculated after 3, 6 and 9 d, respectively. The development, reproduction and population parameters of M. dirhodum were evaluated by using life table test. The mortalities of four aphid species after 3 d were categorized in the order from high to low as follows: Rhopalosiphum padi, Sitobion miscanthi, Schizaphis graminum, M. dirhodum. R.padi and S.miscanthi died out within 6 d, and S. graminum died out within 9 d, while the mortality of M. dirhodum was only 10.3% at 9 d. Most individuals of M. dirhodum completed the entire generation cycle, but delayed developmental time, and significantly declined survival rate, lifespan and fecundity. The intrinsic rate of increase(r), finite rate of increase(λ) and net reproductive rate(R_0) were also found significantly decreased, while mean generation time(T) was significantly prolonged. The results further confirmed that wheat seeds treated with imidacloprid were effective against R. padi, S. avenae, and S. graminum, but poor to M. dirhodum.
Imidacloprid seed coating treatment has been suggested as an important management for wheat aphids in the major grain-producing areas in China. The effect of imidacloprid on the control of aphids was mainly evaluated in mixed natural populations, but few studies were conducted in the laboratory population, especially Metopolophium dirhodum. To determine the effect of imidacloprid seed coating on grain aphids, four aphid species were inoculated on wheat plants seed-treated with imidacloprid, and the aphids mortality was calculated after 3, 6 and 9 d, respectively. The development, reproduction and population parameters of M. dirhodum were evaluated by using life table test. The mortalities of four aphid species after 3 d were categorized in the order from high to low as follows: Rhopalosiphum padi, Sitobion miscanthi, Schizaphis graminum, M. dirhodum. R.padi and S.miscanthi died out within 6 d, and S. graminum died out within 9 d, while the mortality of M. dirhodum was only 10.3% at 9 d. Most individuals of M. dirhodum completed the entire generation cycle, but delayed developmental time, and significantly declined survival rate, lifespan and fecundity. The intrinsic rate of increase(r), finite rate of increase(λ) and net reproductive rate(R_0) were also found significantly decreased, while mean generation time(T) was significantly prolonged. The results further confirmed that wheat seeds treated with imidacloprid were effective against R. padi, S. avenae, and S. graminum, but poor to M. dirhodum.
引文
[1] 杜予州,毛亚勋. 麦无网长管蚜实验种群生命表研究[J]. 贵州农学院学报,1997,16(3):16-21.
[2] DEWAR A, TATCHELL G, TURL L A D. A comparison of cereal aphid migrations over Britain in the summers of 1979 and 1982 [J]. Crop Protection,1984, 3: 379-389.
[3] PONS X, ALBAJES R, AVILA SARASUA M J, et al. Spring population development of cereal aphids on durum wheat in Lleida, NE of Spain [J].Journal of Applied Entomology,1989,107:203-210.
[4] HOWARD M T, DIXON A F G. Forecasting of peak population density of the rose grain aphid Metopolophium dirhodum on wheat [J]. Annals of Applied Biology,1990, 117:9-19.
[5] HONEK A. Factors determining the peak abundance of Metopolophium dirhodum (Homoptera: Aphididae) on cereals [J]. Bulletin of Entomological Research,1991, 81: 57-64.
[6] MA Chunsen, HAU B, POEHLING H M. Effects of pattern and timing of high temperature exposure on reproduction of the rose grain aphid, Metopolophium dirhodum [J]. Entomologia Experimentalis et Applicata, 2004, 110(1): 65-71.
[7] CAROLINA S C, LILIAN R D. Composition and biological activity of essential oils against Metopolophium dirhodum (Hemiptera: Aphididae) cereal crop pest[J]. Pest Management Science, 2012, 68:1492-1500.
[8] 张广学,钟铁森.中国经济昆虫志,第二十五册同翅目蚜虫类(一)[M]. 北京:科学出版社,1983.
[9] 胡胜昌,贡桑. 温度对麦无网长管蚜实验种群生长的影响[J]. 昆虫学报,1985,28(1):36-44.
[10] 陈巨莲,郭予元,倪汉祥,等. 麦无网长管蚜田间种群动态的研究[J].植物保护学报,1994,21(1):7-13.
[11] 王建军, 韩召军, 王荫长. 新烟碱类杀虫剂毒理学研究进展[J]. 植物保护学报, 2001, 28(2): 178-182.
[12] 刘爱芝,李素娟,韩松.吡虫啉包衣对小麦蚜虫的控制效果及增产作用研究初报[J].河南农业科学,2005,34(11): 63-64.
[13] 刘爱芝, 陶岭梅, 韩松,等. 吡虫啉包衣控制全生育期小麦蚜虫有效剂量评价[J]. 植物保护, 2009, 35(2): 152-154.
[14] 郎立宁. 吡虫啉种衣剂对小麦蚜虫的防效及其在小麦上的消解动态[D]. 保定:河北农业大学, 2014.
[15] 王亚欣. 吡虫啉包衣全生育期防控麦蚜技术在不同地区的效果差异及其原因分析[J]. 保定:河北农业大学, 2014.
[16] MIAO Jin, DU Zhenbao, WU Yuqing, et al. Sub-lethal effects of four neonicotinoid seed treatments on the demography and feeding behaviour of the wheat aphid Sitobion avenae [J]. Pest Management Science, 2014, 70(1):55-59.
[17] 邱高辉, 姚远, 韩召军. 麦长管蚜对吡虫啉的抗性机理研究[J]. 南京农业大学学报, 2008, 31(2): 67-70.
[18] 黄钰淞,梁文涛,谢佳燕.麦二叉蚜吡虫啉抗性选育及其3种酶活性变化研究[J].武汉轻工大学学报,2014,33(3):13-15.
[19] ZHANG M, QIAO X F, LI Y T, et al. Cloning of eight Rhopalosiphum padi (Hemiptera: Aphididae) nAChR subunit genes and mutation detection of the β1 subunit in field samples from China [J]. Pesticide Biochemistry and Physiology, 2016, 132: 89-95.
[20] 张云慧, 韩二宾, 李祥瑞, 等. 拌种吡虫啉残留对麦长管蚜实验种群的影响[J]. 昆虫学报, 2013, 56(1): 54-59.
[21] CHI H, SU H Y. Age-stage, two-sex life tables of Aphidius gifuensis (Ashmead) (Hymenoptera: Braconidae) and its host Myzus persicae (Sulzer) (Homoptera: Aphididae) with mathematical proof of the relationship between female fecundity and the net reproductive rate[J]. Environmental Entomology, 2006, 35(1): 10-21.
[22] CHI H. TWOSEX-MSChart: a computer program for the age-stage, two-sex life table analysis. National Chung Hsing University [DB/OL]. http://140.120.197.173/Ecology/Download/TWosex-M Chart.rat/.2016.
[23] 杜光玲, 赤国彤. 种衣剂及其发展应用的研究[J]. 中国农学通报, 2002, 18(1): 52-57.
[24] 党志红, 李耀发, 潘文亮, 等. 吡虫啉包衣防治小麦蚜虫技术及安全性研究[J]. 应用昆虫学报, 2011, 48(6): 1676-1681.
[25] 高占林, 党志红, 李耀发,等. 吡虫啉包衣量对小麦蚜虫的防治效果及其在小麦籽粒中的残留研究[J]. 河北农业科学, 2011, 15(10): 57-59.
[26] 李耀发,党志红,潘文亮,等.新烟碱类杀虫剂噻虫胺拌种防治麦蚜的田间药效及安全性评价[J].农药,2013,52(9):689-691.
[27] 都振宝, 苗进, 武予清,等. 新烟碱类杀虫剂拌种对麦蚜田间防效及药剂残留动态分析[J]. 应用昆虫学报, 2011, 48(6): 1682-1687.
[28] 刘爱芝, 韩松, 梁九进. 新烟碱类杀虫剂拌种防治麦蚜效果及安全性研究[J]. 河南农业科学, 2012, 41(12): 94-97.
[29] 高志山,张学峰,刘海涛,等.新烟碱类杀虫剂种子包衣防治麦蚜的可行性评价[J].植物保护学报,2016,43(5):864-872.
[30] ZHANG Peng, ZHANG Xuefeng, ZHAO Yunhe, et al. Effects of imidacloprid and clothianidin seed treatments on wheat aphids and their natural enemies on winter wheat [J]. Pest Management Science, 2016, 72(6): 1141-1149.
[31] 范文超, 程志, 党志红,等. 吡虫啉包衣对麦长管蚜控制机制的初步探讨[J]. 河北农业大学学报, 2013, 36(1): 90-94.
[32] LU Y H, ZHENG X S, GAO X W. Sublethal effects of imidacloprid on the fecundity, longevity, and enzyme activity of Sitobion avenae (Fabricius) and Rhopalosiphum padi (Linnaeus) [J].Bulletin of Entomological Research,2016,106(4):551-559.
[33] RIX R R, AYYANATH M R, CUTLER G C. Sublethal concentrations of imidacloprid increase reproduction, alter expression of detoxification genes, and prime Myzus persicae, for subsequent stress [J]. Journal of Pest Science,2016, 89(2): 581-589.
[34] RIX R R, CUTLER G C. Does multigenerational exposure to hormetic concentrations of imidacloprid precondition aphids for increased insecticide tolerance?[J]. Pest Management Science, 2018, 74(2): 314-322.
[35] WEBER G. On the ecological genetics of Metopolophiurn dirhodurn (Walker) (Hemiptera, Aphididae)[J]. Journal of Applied Entomology, 1985, 100:451-458.
[2] DEWAR A, TATCHELL G, TURL L A D. A comparison of cereal aphid migrations over Britain in the summers of 1979 and 1982 [J]. Crop Protection,1984, 3: 379-389.
[3] PONS X, ALBAJES R, AVILA SARASUA M J, et al. Spring population development of cereal aphids on durum wheat in Lleida, NE of Spain [J].Journal of Applied Entomology,1989,107:203-210.
[4] HOWARD M T, DIXON A F G. Forecasting of peak population density of the rose grain aphid Metopolophium dirhodum on wheat [J]. Annals of Applied Biology,1990, 117:9-19.
[5] HONEK A. Factors determining the peak abundance of Metopolophium dirhodum (Homoptera: Aphididae) on cereals [J]. Bulletin of Entomological Research,1991, 81: 57-64.
[6] MA Chunsen, HAU B, POEHLING H M. Effects of pattern and timing of high temperature exposure on reproduction of the rose grain aphid, Metopolophium dirhodum [J]. Entomologia Experimentalis et Applicata, 2004, 110(1): 65-71.
[7] CAROLINA S C, LILIAN R D. Composition and biological activity of essential oils against Metopolophium dirhodum (Hemiptera: Aphididae) cereal crop pest[J]. Pest Management Science, 2012, 68:1492-1500.
[8] 张广学,钟铁森.中国经济昆虫志,第二十五册同翅目蚜虫类(一)[M]. 北京:科学出版社,1983.
[9] 胡胜昌,贡桑. 温度对麦无网长管蚜实验种群生长的影响[J]. 昆虫学报,1985,28(1):36-44.
[10] 陈巨莲,郭予元,倪汉祥,等. 麦无网长管蚜田间种群动态的研究[J].植物保护学报,1994,21(1):7-13.
[11] 王建军, 韩召军, 王荫长. 新烟碱类杀虫剂毒理学研究进展[J]. 植物保护学报, 2001, 28(2): 178-182.
[12] 刘爱芝,李素娟,韩松.吡虫啉包衣对小麦蚜虫的控制效果及增产作用研究初报[J].河南农业科学,2005,34(11): 63-64.
[13] 刘爱芝, 陶岭梅, 韩松,等. 吡虫啉包衣控制全生育期小麦蚜虫有效剂量评价[J]. 植物保护, 2009, 35(2): 152-154.
[14] 郎立宁. 吡虫啉种衣剂对小麦蚜虫的防效及其在小麦上的消解动态[D]. 保定:河北农业大学, 2014.
[15] 王亚欣. 吡虫啉包衣全生育期防控麦蚜技术在不同地区的效果差异及其原因分析[J]. 保定:河北农业大学, 2014.
[16] MIAO Jin, DU Zhenbao, WU Yuqing, et al. Sub-lethal effects of four neonicotinoid seed treatments on the demography and feeding behaviour of the wheat aphid Sitobion avenae [J]. Pest Management Science, 2014, 70(1):55-59.
[17] 邱高辉, 姚远, 韩召军. 麦长管蚜对吡虫啉的抗性机理研究[J]. 南京农业大学学报, 2008, 31(2): 67-70.
[18] 黄钰淞,梁文涛,谢佳燕.麦二叉蚜吡虫啉抗性选育及其3种酶活性变化研究[J].武汉轻工大学学报,2014,33(3):13-15.
[19] ZHANG M, QIAO X F, LI Y T, et al. Cloning of eight Rhopalosiphum padi (Hemiptera: Aphididae) nAChR subunit genes and mutation detection of the β1 subunit in field samples from China [J]. Pesticide Biochemistry and Physiology, 2016, 132: 89-95.
[20] 张云慧, 韩二宾, 李祥瑞, 等. 拌种吡虫啉残留对麦长管蚜实验种群的影响[J]. 昆虫学报, 2013, 56(1): 54-59.
[21] CHI H, SU H Y. Age-stage, two-sex life tables of Aphidius gifuensis (Ashmead) (Hymenoptera: Braconidae) and its host Myzus persicae (Sulzer) (Homoptera: Aphididae) with mathematical proof of the relationship between female fecundity and the net reproductive rate[J]. Environmental Entomology, 2006, 35(1): 10-21.
[22] CHI H. TWOSEX-MSChart: a computer program for the age-stage, two-sex life table analysis. National Chung Hsing University [DB/OL]. http://140.120.197.173/Ecology/Download/TWosex-M Chart.rat/.2016.
[23] 杜光玲, 赤国彤. 种衣剂及其发展应用的研究[J]. 中国农学通报, 2002, 18(1): 52-57.
[24] 党志红, 李耀发, 潘文亮, 等. 吡虫啉包衣防治小麦蚜虫技术及安全性研究[J]. 应用昆虫学报, 2011, 48(6): 1676-1681.
[25] 高占林, 党志红, 李耀发,等. 吡虫啉包衣量对小麦蚜虫的防治效果及其在小麦籽粒中的残留研究[J]. 河北农业科学, 2011, 15(10): 57-59.
[26] 李耀发,党志红,潘文亮,等.新烟碱类杀虫剂噻虫胺拌种防治麦蚜的田间药效及安全性评价[J].农药,2013,52(9):689-691.
[27] 都振宝, 苗进, 武予清,等. 新烟碱类杀虫剂拌种对麦蚜田间防效及药剂残留动态分析[J]. 应用昆虫学报, 2011, 48(6): 1682-1687.
[28] 刘爱芝, 韩松, 梁九进. 新烟碱类杀虫剂拌种防治麦蚜效果及安全性研究[J]. 河南农业科学, 2012, 41(12): 94-97.
[29] 高志山,张学峰,刘海涛,等.新烟碱类杀虫剂种子包衣防治麦蚜的可行性评价[J].植物保护学报,2016,43(5):864-872.
[30] ZHANG Peng, ZHANG Xuefeng, ZHAO Yunhe, et al. Effects of imidacloprid and clothianidin seed treatments on wheat aphids and their natural enemies on winter wheat [J]. Pest Management Science, 2016, 72(6): 1141-1149.
[31] 范文超, 程志, 党志红,等. 吡虫啉包衣对麦长管蚜控制机制的初步探讨[J]. 河北农业大学学报, 2013, 36(1): 90-94.
[32] LU Y H, ZHENG X S, GAO X W. Sublethal effects of imidacloprid on the fecundity, longevity, and enzyme activity of Sitobion avenae (Fabricius) and Rhopalosiphum padi (Linnaeus) [J].Bulletin of Entomological Research,2016,106(4):551-559.
[33] RIX R R, AYYANATH M R, CUTLER G C. Sublethal concentrations of imidacloprid increase reproduction, alter expression of detoxification genes, and prime Myzus persicae, for subsequent stress [J]. Journal of Pest Science,2016, 89(2): 581-589.
[34] RIX R R, CUTLER G C. Does multigenerational exposure to hormetic concentrations of imidacloprid precondition aphids for increased insecticide tolerance?[J]. Pest Management Science, 2018, 74(2): 314-322.
[35] WEBER G. On the ecological genetics of Metopolophiurn dirhodurn (Walker) (Hemiptera, Aphididae)[J]. Journal of Applied Entomology, 1985, 100:451-458.