32名RhD变异型献血者分子遗传背景研究
|
摘要
目的对广州地区收集的32例RhD变异型献血者进行基因分型,以了解RhD变异型在广州献血者中的分布情况。方法采用传统血清学方法,对2016年6月—2017年1月的RhD变异型献血者进行RhD表型鉴定。提取基因组DNA,采用MLPA检测试剂盒对收集的32例RhD变异型标本进行RHD基因分型。对于不能明确分型的标本,使用Sanger测序法对RHD基因进行测序分析。使用D-Screen试剂盒对RhD变异型标本的RhD抗原表位行进一步检测。结果在32例RhD变异型标本中:检出RHD~*DVI.3/01N.01 8例、RHD~*DVI.3/DVI.3 1例、RHD~*weak partial 15/01N.01 7例、RHD~*960A/01N.01 3例、RHD~*weak D type 25/01N.01 3例、RHD~*weak D type 72/01N.01 2例、RHD~*D-CE(4)-D-CE(10)/01N.01 1例、RHD~*95A/01N.01 1例、RHD~*710T/01N.01 1例、RHD~*DVI.3/01EL.01 1例、RHD~*weak partial 15/01EL.01 1例、RHD~*weak D type 25/01EL.01 1例、RHD~*DVI.3/D-CE(3-9)-D 1例和RHD~*538A/D-CE(2-9)-D 1例。结论弱部分D15和部分DVI.3是广州地区献血者人群中最常见的RhD变异型。MLPA基因分型方法是1种有助于检出这2种不同RHD突变型等位基因的方法。
Objective To investigate the RHD genotype in the Chinese Guangzhou donors with D variant phenotype by using Multiplex Ligation-dependent Probe Amplification(MLPA) genotyping assay and Sanger sequencing. Methods From June 2016 to January 2017, the blood samples from 32 Chinese Han donors with the D variant phenotype were collected from the Guangzhou Blood Center. RhD variant phenotype was determined using routine serologic methods. The RHD genotype was analyzed by the MLPA assay. Further Sanger sequencing of ten exons of the RHD gene was conducted when RHD genotype can not be determined by the MLPA analysis. The serological characteristics for the epitope of D antigen was further analyzed using the commercial panel anti-D reagents(D-Screen, Diagast). Results RHD~*DVI.3/01 N.01(n=8), RHD~*DVI.3/DVI.3(n=1), RHD~*weak partial 15/01 N.01(n=7), RHD~*960 A/01 N.01(n=3), RHD~*weak D type 25/01 N.01(n=3), RHD~*weak D type 72/01 N.01(n=2), RHD~*D-CE(4)-D-CE(10)/01 N.01(n=1), RHD~*95 A/01 N.01(n=1), RHD~*710 T/01 N.01(n=1), RHD~*DVI.3/01EL.01(n=1), RHD~*weak partial 15/01EL.01(n=1), RHD~*weak D type 25/01 EL.01(n=1), RHD~*DVI.3/D-CE(3-9)-D(n=1) and RHD~*538A/D-CE(2-9)-D(n=1) were identified in 32 donors. Conclusion RHD~*weak partial 15 and RHD~*DVI.3 are the most common RHD alleles accounting for the RhD variant phenotype in the Chinese Guangzhou donors. The MLPA assay is a helpful RHD genotyping method to detect these two RHD alleles.
Objective To investigate the RHD genotype in the Chinese Guangzhou donors with D variant phenotype by using Multiplex Ligation-dependent Probe Amplification(MLPA) genotyping assay and Sanger sequencing. Methods From June 2016 to January 2017, the blood samples from 32 Chinese Han donors with the D variant phenotype were collected from the Guangzhou Blood Center. RhD variant phenotype was determined using routine serologic methods. The RHD genotype was analyzed by the MLPA assay. Further Sanger sequencing of ten exons of the RHD gene was conducted when RHD genotype can not be determined by the MLPA analysis. The serological characteristics for the epitope of D antigen was further analyzed using the commercial panel anti-D reagents(D-Screen, Diagast). Results RHD~*DVI.3/01 N.01(n=8), RHD~*DVI.3/DVI.3(n=1), RHD~*weak partial 15/01 N.01(n=7), RHD~*960 A/01 N.01(n=3), RHD~*weak D type 25/01 N.01(n=3), RHD~*weak D type 72/01 N.01(n=2), RHD~*D-CE(4)-D-CE(10)/01 N.01(n=1), RHD~*95 A/01 N.01(n=1), RHD~*710 T/01 N.01(n=1), RHD~*DVI.3/01EL.01(n=1), RHD~*weak partial 15/01EL.01(n=1), RHD~*weak D type 25/01 EL.01(n=1), RHD~*DVI.3/D-CE(3-9)-D(n=1) and RHD~*538A/D-CE(2-9)-D(n=1) were identified in 32 donors. Conclusion RHD~*weak partial 15 and RHD~*DVI.3 are the most common RHD alleles accounting for the RhD variant phenotype in the Chinese Guangzhou donors. The MLPA assay is a helpful RHD genotyping method to detect these two RHD alleles.
引文
[1] Daniels G.Human blood Groups.3rd ed.Oxford:Wiley-Blackwell,2013.
[2] Flegel WA.The genetics of the Rhesus blood group system.Blood Transfus,2007,5(2):50-57.
[3] Wagner F ,Gassner C,Muller TH,et al.Molecular basis of weak D phenotypes.Blood,1999,93(1):385-393.
[4] Kumpel B.Are weak D RBCs really immunogenic?Transfusion,2006,46(6):1061-1062,1062-1066.
[5] Garratty G.Do we need to be more concerned about weak D antigens?Transfusion,2005,45(10):1547-1551.
[6] Flegel WA.Homing in on D antigen immunogenicity.Transfusion,2005,45(4):466-468.
[7] Wagner FF,Flegel WA.Review:the molecular basis of the Rh blood group phenotypes.Immunohematology,2004,20(1):23-36.
[8] 孙国栋,段现民,尹志柱,等.华北地区汉族人群Rh(D)抗原弱表现型个体的分子遗传机制研究.中国输血杂志,2007(02):108-113.
[9] Ye L,Wang P,Gao H,et al.Partial D phenotypes and genotypes in the Chinese population.Transfusion,2012,52(2):241-246.
[10] Haer-Wigman L,Veldhuisen B,Jonkers R,et al.RHD and RHCE variant and zygosity genotyping via multiplex ligation-dependent probe amplification.Transfusion,2013,53(7):1559-1574.
[11] Ansart-Pirenne H,Asso-Bonnet M,Le Pennec PY,et al.RhD variants in Caucasians:consequences for checking clinically relevant alleles.Transfusion,2004,44(9):1282-1286.
[12] Ji YL,Luo H,Wen JZ,et al.RHD genotype and zygosity analysis in the Chinese Southern Han D+,D- and D variant donors using the multiplex ligation-dependent probe amplification assay.Vox Sang,2017,112(7):660-670.
[13] He J,Ying Y,Hong X,et al.Molecular basis and zygosity determination of D variants including identification of four novel alleles in Chinese individuals.Transfusion,2015,55(1):137-143.
[14] Shao CP,Maas JH,Su YQ,et al.Molecular background of Rh D-positive,D-negative,D(el) and weak D phenotypes in Chinese.Vox Sang,2002,83(2):156-161.
[15] Schouten JP,Mcelgunn CJ,Waaijer R,et al.Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification.Nucleic Acids Res,2002,30(12):e57.
[16] 姬艳丽,魏玲,张润青,等.多重连接依赖的探针扩增(MLPA)技术在广州地区RhD阴性献血者基因分型研究中的应用.中国输血杂志,2012(10):1030-1034.
[17] 骆宏,温机智,张润青,等.RhD变异型个体的表型类型和基因突变机制研究.中国实验血液学杂志,2017(06):1804-1809.
[18] Fichou Y,Le Marechal C,Bryckaert L,et al.Variant screening of the RHD gene in a large cohort of subjects with D phenotype ambiguity:report of 17 novel rare alleles.Transfusion,2012,52(4):759-764.
[19] Ye SH,Wu DZ,Wang MN,et al.A comprehensive investigation of RHD polymorphisms in the Chinese Han population in Xi′an.Blood Transfus,2014,12(3):396-404.
[20] Daniels G.Variants of RhD--current testing and clinical consequences.Br J Haematol,2013,161(4):461-470.
[2] Flegel WA.The genetics of the Rhesus blood group system.Blood Transfus,2007,5(2):50-57.
[3] Wagner F ,Gassner C,Muller TH,et al.Molecular basis of weak D phenotypes.Blood,1999,93(1):385-393.
[4] Kumpel B.Are weak D RBCs really immunogenic?Transfusion,2006,46(6):1061-1062,1062-1066.
[5] Garratty G.Do we need to be more concerned about weak D antigens?Transfusion,2005,45(10):1547-1551.
[6] Flegel WA.Homing in on D antigen immunogenicity.Transfusion,2005,45(4):466-468.
[7] Wagner FF,Flegel WA.Review:the molecular basis of the Rh blood group phenotypes.Immunohematology,2004,20(1):23-36.
[8] 孙国栋,段现民,尹志柱,等.华北地区汉族人群Rh(D)抗原弱表现型个体的分子遗传机制研究.中国输血杂志,2007(02):108-113.
[9] Ye L,Wang P,Gao H,et al.Partial D phenotypes and genotypes in the Chinese population.Transfusion,2012,52(2):241-246.
[10] Haer-Wigman L,Veldhuisen B,Jonkers R,et al.RHD and RHCE variant and zygosity genotyping via multiplex ligation-dependent probe amplification.Transfusion,2013,53(7):1559-1574.
[11] Ansart-Pirenne H,Asso-Bonnet M,Le Pennec PY,et al.RhD variants in Caucasians:consequences for checking clinically relevant alleles.Transfusion,2004,44(9):1282-1286.
[12] Ji YL,Luo H,Wen JZ,et al.RHD genotype and zygosity analysis in the Chinese Southern Han D+,D- and D variant donors using the multiplex ligation-dependent probe amplification assay.Vox Sang,2017,112(7):660-670.
[13] He J,Ying Y,Hong X,et al.Molecular basis and zygosity determination of D variants including identification of four novel alleles in Chinese individuals.Transfusion,2015,55(1):137-143.
[14] Shao CP,Maas JH,Su YQ,et al.Molecular background of Rh D-positive,D-negative,D(el) and weak D phenotypes in Chinese.Vox Sang,2002,83(2):156-161.
[15] Schouten JP,Mcelgunn CJ,Waaijer R,et al.Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification.Nucleic Acids Res,2002,30(12):e57.
[16] 姬艳丽,魏玲,张润青,等.多重连接依赖的探针扩增(MLPA)技术在广州地区RhD阴性献血者基因分型研究中的应用.中国输血杂志,2012(10):1030-1034.
[17] 骆宏,温机智,张润青,等.RhD变异型个体的表型类型和基因突变机制研究.中国实验血液学杂志,2017(06):1804-1809.
[18] Fichou Y,Le Marechal C,Bryckaert L,et al.Variant screening of the RHD gene in a large cohort of subjects with D phenotype ambiguity:report of 17 novel rare alleles.Transfusion,2012,52(4):759-764.
[19] Ye SH,Wu DZ,Wang MN,et al.A comprehensive investigation of RHD polymorphisms in the Chinese Han population in Xi′an.Blood Transfus,2014,12(3):396-404.
[20] Daniels G.Variants of RhD--current testing and clinical consequences.Br J Haematol,2013,161(4):461-470.