胶质重楼石灰水炮制工艺研究
|
摘要
为建立胶质重楼石灰水炮制工艺,通过单因素实验结合正交实验设计,以重楼总皂苷含量为评价指标分别对石灰水浓度、石灰水用量及炮制时间进行考察。结果表明胶质重楼石灰水炮制最佳工艺为:石灰水最佳浓度为0. 1%,用量为30 m L,炮制时间为8 h,在优化条件下,重楼可以从胶质转化为粉质,炮制前、后总皂苷含量分别为1. 85%和1. 88%。石灰水炮制胶质重楼可行,对重楼总皂苷影响较少。
In order to study the processing technology of colloid Paris Polyphylla with limewater,the single factor experiment and the orthogonal experiment were employed to study the concentration,dosage and processing time of limewater with the contents of total saponins as evaluation indexes. The results showed that the optimized conditions were as follows: the concentration of limewater was 0. 1%,the dosage was 30 mL and the processing time was 8 h.Under the optimized conditions,colloid Paris Polyphylla can be transformed to starchy Paris Polyphylla,and the content of total saponins of which was 1. 85% and 1. 88%,respectively. So lime water can be used to process colloid Paris Polyphylla.
In order to study the processing technology of colloid Paris Polyphylla with limewater,the single factor experiment and the orthogonal experiment were employed to study the concentration,dosage and processing time of limewater with the contents of total saponins as evaluation indexes. The results showed that the optimized conditions were as follows: the concentration of limewater was 0. 1%,the dosage was 30 mL and the processing time was 8 h.Under the optimized conditions,colloid Paris Polyphylla can be transformed to starchy Paris Polyphylla,and the content of total saponins of which was 1. 85% and 1. 88%,respectively. So lime water can be used to process colloid Paris Polyphylla.
引文
[1]国家药典委员会.中华人民共和国药典[S].一部.北京:中国医药科技出版社,2015:260.
[2]Xie Z Z,Li M M,Deng P F,et al.Paris saponin-induced autophagy promotes breast cancer cell apoptosis via the Akt/m TOR signaling pathway[J].Chem Biol Interact.,2017,264:1-9.
[2]Watanabe S,Suzuki T,Hara F,et al.Polyphyllin D,a steroidal saponin in Paris polyphylla,apoptosis and necroptosis cell death of neuroblastoma cells[J].Pediatr Surg Int.,2017,33(6):713-719.
[4]Man S,Fan W,Gao W,et al.Anti-fibrosis and anti-cirrhosis effects of Rhizoma paridis saponins on diethylnitrosamine induced rats[J].JEthnopharmacol.,2014,151(1):407-412.
[5]王跃虎,牛红梅,张兆云,等.重楼属植物的药用价值及其化学物质基础[J].中国中药杂志.2015,40(5):833-839.
[6]Wang Y,Gao W Y,Li X,et al.Chemotaxonomic study of the genus Paris based on steroidal saponins[J].Biochem Syst Ecol.,2013,48:163-173.
[7]刘福荣,韩大君.胶质和粉质重楼形成原因初探[J].中药材,1992,15(8):40-41.
[8]易尚平,叶玉兰.胶质重楼和粉质重楼总皂苷含量比较[J].中药材,1989,12(5):34-35.
[9]马云淑,淤泽溥,吕俊,等.胶质重楼与粉质重楼主要药理作用的比较研究[J].中医药研究,1999(1):26-29.
[10]叶玉兰.胶质重楼炮制初探[J].中药材,1991,14(1):35.
[11]李恒,苏豹,张兆云,等.中国重楼资源现状评价及其种植业的发展对策[J].西部林业科学,2015,44(3):1-7.
[12]成莉,甄艳,陈敏,等.扩大重楼药用资源研究进展[J].中国中药杂志,2015,40(16):3121-3124.
[13]年四辉,王扣,罗燕.树脂技术用于重楼总皂苷纯化工艺的研究[J].云南中医中药杂志,2006,27(1):44-45.
[14]王军.半夏毒性及炮制减毒研究[J].陕西中医,2010,31(9):1229-1231.
[15]杨亮,林志伟.马钱子炮制工艺的研究[J].海峡药学,2003,15(1):39-40.
[2]Xie Z Z,Li M M,Deng P F,et al.Paris saponin-induced autophagy promotes breast cancer cell apoptosis via the Akt/m TOR signaling pathway[J].Chem Biol Interact.,2017,264:1-9.
[2]Watanabe S,Suzuki T,Hara F,et al.Polyphyllin D,a steroidal saponin in Paris polyphylla,apoptosis and necroptosis cell death of neuroblastoma cells[J].Pediatr Surg Int.,2017,33(6):713-719.
[4]Man S,Fan W,Gao W,et al.Anti-fibrosis and anti-cirrhosis effects of Rhizoma paridis saponins on diethylnitrosamine induced rats[J].JEthnopharmacol.,2014,151(1):407-412.
[5]王跃虎,牛红梅,张兆云,等.重楼属植物的药用价值及其化学物质基础[J].中国中药杂志.2015,40(5):833-839.
[6]Wang Y,Gao W Y,Li X,et al.Chemotaxonomic study of the genus Paris based on steroidal saponins[J].Biochem Syst Ecol.,2013,48:163-173.
[7]刘福荣,韩大君.胶质和粉质重楼形成原因初探[J].中药材,1992,15(8):40-41.
[8]易尚平,叶玉兰.胶质重楼和粉质重楼总皂苷含量比较[J].中药材,1989,12(5):34-35.
[9]马云淑,淤泽溥,吕俊,等.胶质重楼与粉质重楼主要药理作用的比较研究[J].中医药研究,1999(1):26-29.
[10]叶玉兰.胶质重楼炮制初探[J].中药材,1991,14(1):35.
[11]李恒,苏豹,张兆云,等.中国重楼资源现状评价及其种植业的发展对策[J].西部林业科学,2015,44(3):1-7.
[12]成莉,甄艳,陈敏,等.扩大重楼药用资源研究进展[J].中国中药杂志,2015,40(16):3121-3124.
[13]年四辉,王扣,罗燕.树脂技术用于重楼总皂苷纯化工艺的研究[J].云南中医中药杂志,2006,27(1):44-45.
[14]王军.半夏毒性及炮制减毒研究[J].陕西中医,2010,31(9):1229-1231.
[15]杨亮,林志伟.马钱子炮制工艺的研究[J].海峡药学,2003,15(1):39-40.