七味白术散对菌群失调腹泻小鼠肠黏膜的影响
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摘要
目的探讨七味白术散对抗生素所致菌群失调腹泻小鼠肠黏膜的影响,为七味白术散治疗腹泻的机制提供依据。方法采用头孢拉定和硫酸庆大霉素混合抗生素灌胃制备菌群失调腹泻模型,造模成功后分别灌胃七味白术散传统汤药全量和七味白术散超微50%剂量汤药,治疗3d后采集各组小鼠空肠、回肠和结肠肠段各约2cm,并测定各肠段黏膜淋巴细胞数和肠黏膜厚度。结果与模型组小鼠相比,七味白术散超微50%剂量汤药治疗组和传统汤药治疗组回肠黏膜厚度及各肠段黏膜淋巴细胞数均降低,且回肠黏膜淋巴细胞数的比较差异有统计学意义(t=1.0210,P=0.0480;t=0.9130,P=0.0430);超微50%剂量汤药治疗组小鼠回肠和空肠黏膜的淋巴细胞数显著低于模型组(t=1.0210,P=0.0480;t=1.0031,P=0.0493),更接近正常组。结论七味白术散对抗生素所致菌群失调腹泻小鼠肠道部分肠段黏膜厚度和各肠段黏膜淋巴细胞数有恢复作用,七味白术散超微50%剂量的修复作用优于传统汤药。
Objective To explore the effect of Qiwei Baizhu Powder on the intestinal mucosa of mice with dysbacteria diarrhea.Methods Cefradine and Gentamicin sulphate were orally administered to mice to make the model animals with dysbacteria diarrhea,then the model mice were given traditional decoction of Qiwei Baizhu Powder or 50% dose of ultro-micro Qiwei Baizhu Powder respectively.After 3 days of treatment,the specimens of jejunum,ileum and colon were collected to detect the number of lymphocytes and the thickness of intestinal mucosa.Results Compared with the model group,the thickness of ileum in mice treated with 50% dose of ultro-micro Qiwei Baizhu Powder or traditional decoction of Qiwei Baizhu Powder was lower.and the numbers of lymphocytes in the two treatment groups decreased;the difference in the lymphocyte number in ileum mucosa between the two treatment groups and the model group was significant(t=1.0210,P=0.0480;t=0.9130,P=0.0430).The lymphocyte numbers in ileum mucosa and jejunum mucosa in mice treated with ultro-micro Qiwei Baizhu Powder were evidently lower than those in the model group(t=1.0210,P=0.0480;t=1.0031,P=0.0493),yet was almost at the same level with the control group.Conclusion Qiwei Baizhu Powder can regulate the mucosal thickness and the number of lymphocytes in the intestine of mice with antibiotic dysbacteria diarrhea,and ultra-micro Qiwei Baizhu Powder is more effective than the traditional decoction.
Objective To explore the effect of Qiwei Baizhu Powder on the intestinal mucosa of mice with dysbacteria diarrhea.Methods Cefradine and Gentamicin sulphate were orally administered to mice to make the model animals with dysbacteria diarrhea,then the model mice were given traditional decoction of Qiwei Baizhu Powder or 50% dose of ultro-micro Qiwei Baizhu Powder respectively.After 3 days of treatment,the specimens of jejunum,ileum and colon were collected to detect the number of lymphocytes and the thickness of intestinal mucosa.Results Compared with the model group,the thickness of ileum in mice treated with 50% dose of ultro-micro Qiwei Baizhu Powder or traditional decoction of Qiwei Baizhu Powder was lower.and the numbers of lymphocytes in the two treatment groups decreased;the difference in the lymphocyte number in ileum mucosa between the two treatment groups and the model group was significant(t=1.0210,P=0.0480;t=0.9130,P=0.0430).The lymphocyte numbers in ileum mucosa and jejunum mucosa in mice treated with ultro-micro Qiwei Baizhu Powder were evidently lower than those in the model group(t=1.0210,P=0.0480;t=1.0031,P=0.0493),yet was almost at the same level with the control group.Conclusion Qiwei Baizhu Powder can regulate the mucosal thickness and the number of lymphocytes in the intestine of mice with antibiotic dysbacteria diarrhea,and ultra-micro Qiwei Baizhu Powder is more effective than the traditional decoction.
引文
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[8]LI Jianting,KUANG Zaoyuan,SONG Shanshan,et al.The effect of Wanshibaochiwancombined with antibiotics on the intestine of mice[J].Chin Arch TCM,2010,28(11):2387-2390.(in Chinese)李建婷,邝枣园,宋姗姗,等.王氏保赤丸与抗生素联合用药对小鼠肠道黏膜的影响研究[J].中华中医药学刊,2010,28(11):2387-2390.
[9]SUN Yanzheng,LI Xiaoqiong,ZHOU Dong,et al.Isolation and phenotypic characterization of piglet small intestinal lymphocytes[J].Chin J Vet Sci,2014,34(10):1627-1633.(in Chinese)孙艳争,李小琼,周栋,等.仔猪小肠黏膜淋巴细胞的分离及表型鉴定[J].中国兽医学报,2014,34(10):1627-1633.
[10]LI Zhijin,ZHAN Liying,HU Jun,et al.Effects of Dexamethason on apoptosis of peripheral blood lymphocytes and lamina propria lymphocytes in ulcerative colitis rats[J].Jiangxi Med J,2013,48(5):386-387,396.(in Chinese)李志晋,詹丽英,胡俊,等.地塞米松对溃疡性结肠炎鼠外周血及肠黏膜固有层淋巴细胞凋亡的影响[J].江西医药,2013,48(5):386-387,396.
[11]YAN Huimin,CHANG Hui.Characterization analysis of intestinal lamina propria lymphocyte subsets[J].Chin J Mod Med,2013,23(12):11-14.(in Chinese)闫会敏,常晖.小肠黏膜固有层淋巴细胞的免疫学分析[J].中国现代医学杂志,2013,23(12):11-14.
[12]Morgun A,Dzutsev A,Dong X,et al.Uncovering effects of antibiotics on the host and microbiota using transkingdom gene networks[J].Gut,2015,64(11):1732-1742.
[13]LIU Qisheng,LIU Huai,PENG Wei,et al.The influence of antibiotic modeling on intestinal mucosa in dysbacteriotic diarrhea mice[J].Chin J Microecol,2015,27(5):501-504,512.(in Chinese)刘起胜,刘怀,彭微,等.菌群失调腹泻抗生素造模对小鼠肠黏膜的影响[J].中国微生态学杂志,2015,27(5):501-504,512.
[14]SUN Biqiang,WU Canrong,ZHOU Ying,et al.Effects of different formulations of Qiwei Baizhu Powder on the ultrastructure of small intestinal mucosa and sIgA in mice with intestinal dysbacteriosis[J].Chin J Microecol,2016,28(2):125-128,137.(in Chinese)孙必强,伍参荣,周英,等.不同剂型七味白术散对肠道菌群失调腹泻小鼠小肠黏膜超微结构和sIgA的影响[J].中国微生态学杂志,2016,28(2):125-128,137.
[15]SUN Biqiang,ZHOU Ying,LIU Weidong,et al.Effect of different formulated qiwei baizhu san on mRNA expression of IL-4,IL-10and IFN-αin diarrhea mice with intestinal flora alteration[J].Chin J Exp Tradit Med Form,2016,22(6):84-88.(in Chinese)孙必强,周英,刘卫东,等.不同剂型七味白术散对肠道菌群失调腹泻小鼠小肠黏膜上皮IL-4,IL-10,IFN-αmRNA表达的影响[J].中国实验方剂学杂志,2016,22(6):84-88.
[16]SUN Biqiang,ZHOU Ying,LIU Weidong,et al.Effects of different formulated Qiwei Baizhu San on intestinal mucosa and TNF-α,IL-6in serum of mice with intestinal flora[J].Chin J Exp Tradit Med Form,2016,22(4):95-100.(in Chinese)孙必强,周英,刘卫东,等.不同剂型七味白术散对肠道菌群失调小鼠小肠黏膜和血清TNF-α、IL-6的影响[J].中国实验方剂学杂志,2016,22(4):95-100.
[17]LIU Qisheng,XU Xiaohong,LIU Huai,et al.Effects on intestinal villi and crypts in flora diarrhea mice by Qiweibaizhu Powder[J].Chin Med Mod Dist Educ China,2014,12(23):154-155.(in Chinese)刘起胜,徐筱红,刘怀,等.七味白术散对菌群失调腹泻小鼠肠绒毛和隐窝的影响[J].中国中医药现代远程教育,2014,12(23):154-155.
[18]ZHANG Hualing,ZHOU Sainan,CAI Ying,et al.The effect of ultro-micro Qiweibaizhusan on the mucous membrane of small intestinal of diarrheal mice with dysbacteriosis[J].Chin J Microecol,2013,25(1):9-13.(in Chinese)张华玲,周赛男,蔡莹,等.超微七味白术散对菌群失调腹泻小鼠小肠黏膜的影响[J].中国微生态学杂志,2013,25(1):9-13.
[19]HUI Huaying,HE Lu,PENG Xinxin,et al.The influence of antibiotics modeling on the intestinal lactase activity in dysbacteriotic diarrhea mice[J].Chin J Prevent Vet Med,2017,39(9):761-763.(in Chinese)惠华英,贺璐,彭昕欣,等.抗生素所致菌群失调腹泻小鼠肠道乳糖酶活性的研究[J].中国预防兽医学报,2017,39(9):761-763.
[2]DENG Hongjie,WU Hai,PENG Xiaoshan,et al.Effects of ultra-micro Powder of Qiweibaizhu on dysbacteriosis in mice with diarrhea[J].Prog Mod Biomed,2011,11(19):3632-3635.(in Chinese)邓红洁,吴海,彭晓珊,等.超微七味白术散对菌群失调小鼠的疗效研究[J].现代生物医学进展,2011,11(19):3632-3635.
[3]ZENG Ao,ZHANG Hualing,TAN Zhoujin,et al.The construction of mice diarrhea model due to dysbacteriosis and curative effect of ultra-micro Qiweibaizhusan[J].Microbiol China,2012,39(9):1341-1348.(in Chinese)曾奥,张华玲,谭周进,等.小鼠菌群失调腹泻模型的建立及超微七味白术散的疗效[J].微生物学通报,2012,39(9):1341-1348.
[4]李春颖.七味白术散加味治疗抗生素相关性腹泻疗效观察[J].中日友好医院学报,2010,24(5):292.
[5]邓中甲,李冀,连建伟,等.方剂学[M].北京:中国中医药出版社,2009:154.
[6]TAN Zhoujin,WU Hai,LIU Fulin,et al.Effect of ultra-micro Powder Qiweibaishusanon the intestinal microbiota and enzyme activities in mice[J].Acta Ecol Sin,2012,32(21):6856-6863.(in Chinese)谭周进,吴海,刘富林,等.超微七味白术散对肠道微生物及酶活性的影响[J].生态学报,2012,32(21):6856-6863.
[7]YANG Bo,WU Canrong,TANG Xiaomei,et al.Influence of Qiweibaizhu Powder on the serum IFN-γand TNF-αlevels of sucking mice infected with human rotavirus(HRV)[J].Chin J Inform TCM,2005,12(3):36-37.(in Chinese)杨波,伍参荣,唐小梅,等.七味白术散对人轮状病毒感染乳鼠血清IFN-γ、TNF-α的影响[J].中国中医药信息杂志,2005,12(3):36-37.
[8]LI Jianting,KUANG Zaoyuan,SONG Shanshan,et al.The effect of Wanshibaochiwancombined with antibiotics on the intestine of mice[J].Chin Arch TCM,2010,28(11):2387-2390.(in Chinese)李建婷,邝枣园,宋姗姗,等.王氏保赤丸与抗生素联合用药对小鼠肠道黏膜的影响研究[J].中华中医药学刊,2010,28(11):2387-2390.
[9]SUN Yanzheng,LI Xiaoqiong,ZHOU Dong,et al.Isolation and phenotypic characterization of piglet small intestinal lymphocytes[J].Chin J Vet Sci,2014,34(10):1627-1633.(in Chinese)孙艳争,李小琼,周栋,等.仔猪小肠黏膜淋巴细胞的分离及表型鉴定[J].中国兽医学报,2014,34(10):1627-1633.
[10]LI Zhijin,ZHAN Liying,HU Jun,et al.Effects of Dexamethason on apoptosis of peripheral blood lymphocytes and lamina propria lymphocytes in ulcerative colitis rats[J].Jiangxi Med J,2013,48(5):386-387,396.(in Chinese)李志晋,詹丽英,胡俊,等.地塞米松对溃疡性结肠炎鼠外周血及肠黏膜固有层淋巴细胞凋亡的影响[J].江西医药,2013,48(5):386-387,396.
[11]YAN Huimin,CHANG Hui.Characterization analysis of intestinal lamina propria lymphocyte subsets[J].Chin J Mod Med,2013,23(12):11-14.(in Chinese)闫会敏,常晖.小肠黏膜固有层淋巴细胞的免疫学分析[J].中国现代医学杂志,2013,23(12):11-14.
[12]Morgun A,Dzutsev A,Dong X,et al.Uncovering effects of antibiotics on the host and microbiota using transkingdom gene networks[J].Gut,2015,64(11):1732-1742.
[13]LIU Qisheng,LIU Huai,PENG Wei,et al.The influence of antibiotic modeling on intestinal mucosa in dysbacteriotic diarrhea mice[J].Chin J Microecol,2015,27(5):501-504,512.(in Chinese)刘起胜,刘怀,彭微,等.菌群失调腹泻抗生素造模对小鼠肠黏膜的影响[J].中国微生态学杂志,2015,27(5):501-504,512.
[14]SUN Biqiang,WU Canrong,ZHOU Ying,et al.Effects of different formulations of Qiwei Baizhu Powder on the ultrastructure of small intestinal mucosa and sIgA in mice with intestinal dysbacteriosis[J].Chin J Microecol,2016,28(2):125-128,137.(in Chinese)孙必强,伍参荣,周英,等.不同剂型七味白术散对肠道菌群失调腹泻小鼠小肠黏膜超微结构和sIgA的影响[J].中国微生态学杂志,2016,28(2):125-128,137.
[15]SUN Biqiang,ZHOU Ying,LIU Weidong,et al.Effect of different formulated qiwei baizhu san on mRNA expression of IL-4,IL-10and IFN-αin diarrhea mice with intestinal flora alteration[J].Chin J Exp Tradit Med Form,2016,22(6):84-88.(in Chinese)孙必强,周英,刘卫东,等.不同剂型七味白术散对肠道菌群失调腹泻小鼠小肠黏膜上皮IL-4,IL-10,IFN-αmRNA表达的影响[J].中国实验方剂学杂志,2016,22(6):84-88.
[16]SUN Biqiang,ZHOU Ying,LIU Weidong,et al.Effects of different formulated Qiwei Baizhu San on intestinal mucosa and TNF-α,IL-6in serum of mice with intestinal flora[J].Chin J Exp Tradit Med Form,2016,22(4):95-100.(in Chinese)孙必强,周英,刘卫东,等.不同剂型七味白术散对肠道菌群失调小鼠小肠黏膜和血清TNF-α、IL-6的影响[J].中国实验方剂学杂志,2016,22(4):95-100.
[17]LIU Qisheng,XU Xiaohong,LIU Huai,et al.Effects on intestinal villi and crypts in flora diarrhea mice by Qiweibaizhu Powder[J].Chin Med Mod Dist Educ China,2014,12(23):154-155.(in Chinese)刘起胜,徐筱红,刘怀,等.七味白术散对菌群失调腹泻小鼠肠绒毛和隐窝的影响[J].中国中医药现代远程教育,2014,12(23):154-155.
[18]ZHANG Hualing,ZHOU Sainan,CAI Ying,et al.The effect of ultro-micro Qiweibaizhusan on the mucous membrane of small intestinal of diarrheal mice with dysbacteriosis[J].Chin J Microecol,2013,25(1):9-13.(in Chinese)张华玲,周赛男,蔡莹,等.超微七味白术散对菌群失调腹泻小鼠小肠黏膜的影响[J].中国微生态学杂志,2013,25(1):9-13.
[19]HUI Huaying,HE Lu,PENG Xinxin,et al.The influence of antibiotics modeling on the intestinal lactase activity in dysbacteriotic diarrhea mice[J].Chin J Prevent Vet Med,2017,39(9):761-763.(in Chinese)惠华英,贺璐,彭昕欣,等.抗生素所致菌群失调腹泻小鼠肠道乳糖酶活性的研究[J].中国预防兽医学报,2017,39(9):761-763.