结直肠癌组织中HIF-1α与miR-138的表达变化及其与患者预后的相关性探讨
|
摘要
目的探讨结直肠癌组织中缺氧诱导因子-1a(HIF-1a)与micro RNA-138(mi R138)的表达变化及与其患者预后的相关性。方法选择2016年1月~2017年8月科室收治的结直肠患者70例,设为观察组,患者行手术治疗,术中取结直肠癌病灶组织;以癌旁组织设为对照组。采用免疫组织化学法完成两组HIF-1α与mi R138蛋白表达测定,记录结直肠癌组织患者病理资料,包括:年龄、肿瘤直径、肿瘤分期、分化程度、淋巴结转移及术后复发情况,对上述病理资料进行单因素分析;采用COX回归模型分析结直肠癌组织中HIF-1α与mi R138与患者预后的相关性。结果观察组结肠癌组织中HIF-1α与mi R138阳性率,均高于对照组(P<0.05);单因素及COX回归模型分析结果表明:结直肠癌组织中HIF-1α与mi R138阳性率与性别、年龄无相关性(P>0.05);结直肠癌组织中HIF-1α与mi R138阳性率与肿瘤大小、肿瘤分期、淋巴结转移、分化程度及浸润呈正相关性(P<0.05)。结论结直肠癌组织中HIF-1α与mi R138均呈高表达,其表达水平与患者预后具有一定的相关性,加强患者HIF-1α与mi R138水平测定能评估患者预后,值得推广应用。
Objective To investigate the expression of hypoxia-inducible factor-1α(HIF-1α) and micro RNA-138(mi R138) in colorectal cancer and its correlation with prognosis. Methods A total of 70 colorectal patients were selected from January 2016 to August 2017 as the observation group. The patients were treated with surgery and the colorectal cancer tissue was taken during the operation. The adjacent tissue was used as a control group. The expression of HIF-1α and mi R138 protein in the two groups was determined by immunohistochemical method. The pathological data of colorectal cancer tissues were recorded including age, tumor diameter, tumor stage, differentiation, lymph node metastasis and postoperative recurrence. Data were analyzed by univariate analysis; COX regression models were used to analyze the association between HIF-1α and mi R138 in patients with colorectal cancer and their prognosis. Results The positive rates of HIF-1α and mi R138 in the observation group were higher than those in the control group(P<0.05).The results of univariate and COX regression analysis showed that there was no correlation between the positive rates of HIF-1α and mi R138 in colorectal cancer tissues and gender, age(P>0.05). The positive rates of HIF-1α and mi R138 in colorectal cancer tissues were positively correlated with tumor size, tumor stage, lymph node metastasis, differentiation and infiltration(P<0.05). Conclusion The expressions of HIF-1α and mi R138 in colorectal cancer tissue are high. The expression level of HIF-1α and mi R138 is correlated with the prognosis of patients. Strengthening the measurement of HIF-1α and mi R138 levels can evaluate the prognosis of patients and is worthy of popularization and application.
Objective To investigate the expression of hypoxia-inducible factor-1α(HIF-1α) and micro RNA-138(mi R138) in colorectal cancer and its correlation with prognosis. Methods A total of 70 colorectal patients were selected from January 2016 to August 2017 as the observation group. The patients were treated with surgery and the colorectal cancer tissue was taken during the operation. The adjacent tissue was used as a control group. The expression of HIF-1α and mi R138 protein in the two groups was determined by immunohistochemical method. The pathological data of colorectal cancer tissues were recorded including age, tumor diameter, tumor stage, differentiation, lymph node metastasis and postoperative recurrence. Data were analyzed by univariate analysis; COX regression models were used to analyze the association between HIF-1α and mi R138 in patients with colorectal cancer and their prognosis. Results The positive rates of HIF-1α and mi R138 in the observation group were higher than those in the control group(P<0.05).The results of univariate and COX regression analysis showed that there was no correlation between the positive rates of HIF-1α and mi R138 in colorectal cancer tissues and gender, age(P>0.05). The positive rates of HIF-1α and mi R138 in colorectal cancer tissues were positively correlated with tumor size, tumor stage, lymph node metastasis, differentiation and infiltration(P<0.05). Conclusion The expressions of HIF-1α and mi R138 in colorectal cancer tissue are high. The expression level of HIF-1α and mi R138 is correlated with the prognosis of patients. Strengthening the measurement of HIF-1α and mi R138 levels can evaluate the prognosis of patients and is worthy of popularization and application.
引文
[1]葛丽卫,谢伟峰,顾金华,等.结直肠癌患者组织中HIF-1α和GLS2表达水平与病理程度和预后的相关性[J].中华普外科手术学杂志:电子版,2017,11(3):265-268.
[2]李妮,谢赣丰,李文花,等.缺氧诱导因子1α和2型谷氨酰胺酶在结直肠癌中的表达及临床意义[J].第三军医大学学报,2016,38(5):496-500.
[3]Tuomisto A,García-Solano J,Sirni觟P,et al.HIF-1αexpression and high microvessel density are characteris tic features in serrated colorectal cancer[J].Virchows Archiv,2016,469(4):1-10.
[4]宋军民,杨超,李震,等.微小RNA-155和WEE1在结肠癌中的作用及其表达的相关性[J].中华实验外科杂志,2015,32(2):397-399.
[5]郭靖,尚杨杨,汪荣泉.结直肠癌及缺氧的结直肠癌细胞中HIF-1α与mi RNA-200b表达的相关性[J].第三军医大学学报,2016,38(6):545-549.
[6]王茜,时延龙,毕经旺.p-m TOR与HIF-1α在结直肠癌组织中的表达及其临床意义[J].中国肿瘤生物治疗杂志,2015,22(6):779-784.
[7]Shang Y,Chen H,Ye J,et al.HIF-1/Ascl2/mi R-200b regulatory feedback circuit modulated the epithelialmesenchymal transition(EMT)in colorectal cancer cells[J].Experimental Cell Research,2017,360(2):243.
[8]赵继明,彭健.Micro RNA-132在结肠癌中的表达及其临床意义[J].中国现代医学杂志,2016,26(5):48-52.
[9]张丽静,王园园,朱娜娜,等.结直肠癌中mi R-139-5p与TGIF1的表达及相关性的研究[J].肿瘤,2015,35(10):1127-1134.
[10]杨通印,郝朗松,郭德正.缺氧诱导因子1α、葡萄糖转运蛋白1和乳酸脱氢酶5在结直肠癌的表达及其意义[J].中华病理学杂志,2017,46(2):93-97.
[11]Tanaka S,Hosokawa M,Ueda K,et al.Effects of decitabine on invasion and exosomal expression of mi R-200c and mi R-141 in oxaliplatin-resistant colorectal cancer cells[J].Biological&Pharmaceutical Bulletin,2015,38(9):1272-1279.
[12]惠菲菲,刘凯,杨家和,等.同时性结直肠癌肝转移结直肠癌组织中Kiss-1蛋白和基质金属蛋白酶9蛋白的表达及意义[J].中华消化外科杂志,2016,15(2):153-160.
[13]秦乐,曾妍.E-cadherin缺失表达与结直肠癌患者预后相关性多元Cox回归分析[J].中国临床药理学杂志,2017,33(1):7-9.
[14]Tanaka S,Hosokawa M,Yonezawa T,et al.Induction of epithelial-mesenchymal transition and down-regulation of mi R-200c and mi R-141 in oxaliplatin-resistant colorectal cancer cells[J].Biological&Pharmaceutical Bulletin,2015,38(3):435-440.
[15]Hsiao KY,Wu MH,Chang N,et al.Coordination of AUF1 and mi R-148a to destabilize DNA methyltransferase 1 m RNA under hypoxia in endometriosis[J].Molecular Human Reproduction,2015,21(12):894.
[16]Bustamante-Marin XM,Cook MS,Gooding J,et al.Leftbiased spermatogenic failure in 129/Sv J Dnd1Ter/+mice correlates with differences in vascular architecture,oxygen availability,and metabolites[J].Biology of Reproduction,2015,93(3):78.
[17]武雪亮,薛军,王立坤,等.DNA结合分化抑制蛋白1和基质金属蛋白酶9在结直肠癌中的表达及与微血管密度的相关性[J].中国医学科学院学报,2016,38(6):696-701.
[18]周仲国,王若婧,潘志忠,等.Pim-3在结直肠癌中的表达及其与预后的关系[J].广东医学,2015,36(23):3627-3630.
[19]汪毅,袁伟,马肖,等.mi R-152在结直肠癌组织中的表达及其与预后的关系[J].中华肿瘤杂志,2016,38(10):763-766.
[20]武雪亮,王立坤,薛军,等.结直肠癌组织中DNA结合分化抑制蛋白1和MMP-9的表达及其与LMVD的相关性[J].肿瘤防治研究,2016,43(11):959-963.
[2]李妮,谢赣丰,李文花,等.缺氧诱导因子1α和2型谷氨酰胺酶在结直肠癌中的表达及临床意义[J].第三军医大学学报,2016,38(5):496-500.
[3]Tuomisto A,García-Solano J,Sirni觟P,et al.HIF-1αexpression and high microvessel density are characteris tic features in serrated colorectal cancer[J].Virchows Archiv,2016,469(4):1-10.
[4]宋军民,杨超,李震,等.微小RNA-155和WEE1在结肠癌中的作用及其表达的相关性[J].中华实验外科杂志,2015,32(2):397-399.
[5]郭靖,尚杨杨,汪荣泉.结直肠癌及缺氧的结直肠癌细胞中HIF-1α与mi RNA-200b表达的相关性[J].第三军医大学学报,2016,38(6):545-549.
[6]王茜,时延龙,毕经旺.p-m TOR与HIF-1α在结直肠癌组织中的表达及其临床意义[J].中国肿瘤生物治疗杂志,2015,22(6):779-784.
[7]Shang Y,Chen H,Ye J,et al.HIF-1/Ascl2/mi R-200b regulatory feedback circuit modulated the epithelialmesenchymal transition(EMT)in colorectal cancer cells[J].Experimental Cell Research,2017,360(2):243.
[8]赵继明,彭健.Micro RNA-132在结肠癌中的表达及其临床意义[J].中国现代医学杂志,2016,26(5):48-52.
[9]张丽静,王园园,朱娜娜,等.结直肠癌中mi R-139-5p与TGIF1的表达及相关性的研究[J].肿瘤,2015,35(10):1127-1134.
[10]杨通印,郝朗松,郭德正.缺氧诱导因子1α、葡萄糖转运蛋白1和乳酸脱氢酶5在结直肠癌的表达及其意义[J].中华病理学杂志,2017,46(2):93-97.
[11]Tanaka S,Hosokawa M,Ueda K,et al.Effects of decitabine on invasion and exosomal expression of mi R-200c and mi R-141 in oxaliplatin-resistant colorectal cancer cells[J].Biological&Pharmaceutical Bulletin,2015,38(9):1272-1279.
[12]惠菲菲,刘凯,杨家和,等.同时性结直肠癌肝转移结直肠癌组织中Kiss-1蛋白和基质金属蛋白酶9蛋白的表达及意义[J].中华消化外科杂志,2016,15(2):153-160.
[13]秦乐,曾妍.E-cadherin缺失表达与结直肠癌患者预后相关性多元Cox回归分析[J].中国临床药理学杂志,2017,33(1):7-9.
[14]Tanaka S,Hosokawa M,Yonezawa T,et al.Induction of epithelial-mesenchymal transition and down-regulation of mi R-200c and mi R-141 in oxaliplatin-resistant colorectal cancer cells[J].Biological&Pharmaceutical Bulletin,2015,38(3):435-440.
[15]Hsiao KY,Wu MH,Chang N,et al.Coordination of AUF1 and mi R-148a to destabilize DNA methyltransferase 1 m RNA under hypoxia in endometriosis[J].Molecular Human Reproduction,2015,21(12):894.
[16]Bustamante-Marin XM,Cook MS,Gooding J,et al.Leftbiased spermatogenic failure in 129/Sv J Dnd1Ter/+mice correlates with differences in vascular architecture,oxygen availability,and metabolites[J].Biology of Reproduction,2015,93(3):78.
[17]武雪亮,薛军,王立坤,等.DNA结合分化抑制蛋白1和基质金属蛋白酶9在结直肠癌中的表达及与微血管密度的相关性[J].中国医学科学院学报,2016,38(6):696-701.
[18]周仲国,王若婧,潘志忠,等.Pim-3在结直肠癌中的表达及其与预后的关系[J].广东医学,2015,36(23):3627-3630.
[19]汪毅,袁伟,马肖,等.mi R-152在结直肠癌组织中的表达及其与预后的关系[J].中华肿瘤杂志,2016,38(10):763-766.
[20]武雪亮,王立坤,薛军,等.结直肠癌组织中DNA结合分化抑制蛋白1和MMP-9的表达及其与LMVD的相关性[J].肿瘤防治研究,2016,43(11):959-963.