江苏省2017年禽流感流行病学调查及分析
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摘要
为了解2017年江苏省禽流感的流行变异情况,共采集鸡、鸭、鹅拭子2760份,均过滤接种SPF鸡胚用以分离病毒。另选取4株宿主来源和分离地点不同的H9N2阳性样品,对其HA和PB_2基因进行测序,以分析H9N2的变异情况。结果表明:禽流感病毒的分离有两个高峰期,即4～6月、9～11月;H5、H7亚型禽流感病毒的感染率较低。4株H9N2禽流感病毒呈现出典型的人流感受体结合特性,但尚未具有突破种间屏障感染哺乳动物的能力。
In order to understand the prevalence and variation status of Avian Influenza Virus(AIV) in Jiangsu province in 2017, we collected 2760 swab samples from chickens, ducks and geese to inoculate SPF chicken embryos after filtration, and selected four isolates of H9 N2 AIV from different hosts at various locations to sequence their HA and PB_2 genes. The results showed that: the AIV isolation had two peak stages in a year, namely from April to June, and from September to November; the infection rate of H5 and H7-subtype AIV was relatively low. The four H9 N2 AIV isolates exhibited typical human influenza receptor binding characteristics, but they could not yet break through the interspecific barrier to infect mammals.
In order to understand the prevalence and variation status of Avian Influenza Virus(AIV) in Jiangsu province in 2017, we collected 2760 swab samples from chickens, ducks and geese to inoculate SPF chicken embryos after filtration, and selected four isolates of H9 N2 AIV from different hosts at various locations to sequence their HA and PB_2 genes. The results showed that: the AIV isolation had two peak stages in a year, namely from April to June, and from September to November; the infection rate of H5 and H7-subtype AIV was relatively low. The four H9 N2 AIV isolates exhibited typical human influenza receptor binding characteristics, but they could not yet break through the interspecific barrier to infect mammals.
引文
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[2] Wu Y,Wu Y,Tefsen B,et al.Bat-derived influenza-like viruses H17N10 and HI8N11 [J].Trends Microbiol,2014,22(4):183-91.
[3] Perdue M L,Suarez D L.Structural features of the avian influenza virus hemagglutinin that influences virulence [J].Vet Microbiol,2000,74(1-2):77-86.
[4] Butt K M,Smith G J,Chen H,et al.Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003 [J].J Clin Microbiol,2005,43(11):5760-5767.
[5] Gao R,Cao B,Shu Y,et al.Human infection with a novel avian-origin influenza A (H7N9) virus [J].N Engl J Med,2013,368(20):1888-1897.
[6] 张烨,李晓丹,邹淑梅,等.2009～2013年我国活禽市场环境样本中禽流感病毒的检测[J].病毒学报,2015,31(6):615-619.
[7] 金元昌,李景鹏,张龙,等.禽流感病毒分子生物学研究进展[J].动物医学进展,2003,24(1):12-15.
[8] Li M L,Rao P,Krug R M.The active sites of the influenza cap-dependent endonuclease are on different polymerase subunits [J].EMBO J,2001,20:2078-2086.
[9] Neumann G,Brownlee G G,Fodor E,et al.Orthomyxovirus replication,transcription,and polyadenylation [J].Curr Top Microbiol Immunol,2004,283:121-143.
[10] Gonzalez S,Ortin J.Distinct regions of influenza virus PB1 polymerase subunit recognize vRNA and cRNA templates [J].EMBO J,1999,18:3767-3775.
[11] Wan H,Perez D.Amino acid 226 in the hemagglutinin of H9N2 influenza viruses determines cell tropism and replication in human airway epithelial cells [J].Virology,2007,81(10):5181-5191.
[12] Hatta M,Neumann G,Kawaoka Y.Reverse genetics approach towards understanding pathogenesis of H5N1 Hong Kong influenza A virus infection [J].Philos Trans R Soclond B Biol Sci,2001,356(1416):1841-1843.
[13] Sun Y,Pu J,Liu J,et al.Genotypic evolution and antigenic drift of H9N2 influenza viruses in China from 1994 to 2008 [J].Veterinary Microbiology,2010,146(3-4):215-225.
[14] 杨婧,刘宇卓,赵冬敏,等.2013年苏皖地区4株H9N2亚型禽流感病毒HA、NA基因的遗传演化分析[J].浙江农业学报,2015,27(11):1896-1902.