HPLC-DAD法测定八味茵术颗粒剂中6,7-二甲氧基香豆素、白术内酯Ⅲ、白术内酯Ⅰ的含量
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摘要
目的:探讨八味茵术颗粒剂中6,7-二甲氧基香豆素、白术内酯Ⅲ、白术内酯Ⅰ的含量测定方法。方法:选取DIKMA Diamonsil C_(18)(2)色谱柱(4. 6 mm×250 mm,5μm),柱温30℃,DAD检测(200～400 nm):6,7-二甲氧基香豆素的检测波长为340 nm,白术内酯Ⅲ的检测波长为220 nm,白术内酯Ⅰ的检测波长为275 nm。进样量为5μL,流动相为水(A)-乙腈(B)梯度洗脱,流速为1 mL/min。结果:6,7-二甲氧基香豆素在0. 1～100. 0μg/mL范围内线性关系良好(R~2=1. 0000)、白术内酯Ⅲ在0. 1～100. 0μg/mL范围内线性关系良好(R~2=1. 0000)、白术内酯Ⅰ在0. 1～100. 0μg/mL范围内线性关系良好(R~2=1. 0000),其精密度、稳定性、加样回收率均符合含量测定要求。结论:本方法简单易行,准确可靠,可有效用于八味茵术颗粒剂的质量控制。
Objective: To establish the HPLC method for determining the contents of 6,7-dimethoxycoumarin,atractylenolide Ⅲ and atractylenolide I in Bawei Yinzhu Granules. Methods: The DIKMA Diamonsil C_(18) (2) (4. 6 mm × 250 mm,5 μm) column was adopted to separate the sample at the column temperature of 30 ℃. The detection wavelength was set between 200 nm to 400 nm. The detection wavelength of 6,7-dimethoxycoumarin,atractylenolide III and atractylenolide I were 340 nm,220 nm and 275 nm respectively. The injection volume was 5 μL. The mobile phase consisted of water-acetonitrile with a gradient elution at a flow rate of 1. 0 m L/min. Results: The calibration curves of were linear in the ranges of 0. 1-100. 0 μg/m L for 6,7-dimethoxycoumarin (R~2= 1. 0000); the calibration curves were linear in the ranges of 0. 1-100. 0 μg/m L for atractylenolide Ⅲ (R~2= 1. 0000); and the calibration curves were linear in the ranges of 0. 1-100. 0 μg/m L for atractylenolideⅠ(R~2= 1. 0000). The precision,accuracy and recovery met the determination requirements. Conclusion: The method is simple and feasible,and thus can be used for the quality control of Bawei Yinzhu Granules.
Objective: To establish the HPLC method for determining the contents of 6,7-dimethoxycoumarin,atractylenolide Ⅲ and atractylenolide I in Bawei Yinzhu Granules. Methods: The DIKMA Diamonsil C_(18) (2) (4. 6 mm × 250 mm,5 μm) column was adopted to separate the sample at the column temperature of 30 ℃. The detection wavelength was set between 200 nm to 400 nm. The detection wavelength of 6,7-dimethoxycoumarin,atractylenolide III and atractylenolide I were 340 nm,220 nm and 275 nm respectively. The injection volume was 5 μL. The mobile phase consisted of water-acetonitrile with a gradient elution at a flow rate of 1. 0 m L/min. Results: The calibration curves of were linear in the ranges of 0. 1-100. 0 μg/m L for 6,7-dimethoxycoumarin (R~2= 1. 0000); the calibration curves were linear in the ranges of 0. 1-100. 0 μg/m L for atractylenolide Ⅲ (R~2= 1. 0000); and the calibration curves were linear in the ranges of 0. 1-100. 0 μg/m L for atractylenolideⅠ(R~2= 1. 0000). The precision,accuracy and recovery met the determination requirements. Conclusion: The method is simple and feasible,and thus can be used for the quality control of Bawei Yinzhu Granules.
引文
[1]王嫦鹤,段海洁,贺浪冲.白术内酯Ⅰ大鼠在体肠吸收动力学研究[J].中国中药杂志,2009,34(11):1430-1434.
[2]王涵,杨娜,谭静,等.白术化学成分、药理作用及临床应用的研究进展[J].甘肃医药,2018,37(1):23-26.
[3]姜淋洁,付涛,卢锟刚,等.白术提取物对大鼠预防性调血脂及保肝作用的实验研究[J].数理医药学杂志,2011,24(4):398-401.
[4]向小庆,叶红.白术抗肿瘤作用的研究及应用进展[J].中国实验方剂学杂志,2013,19(8):367-370.
[5]李雯,尹华.白术化学成分的药理作用研究进展[J].海峡药学,2012,24(3):9-11.
[6]施文荣,刘艳,陈玲,等.白术燥湿利水作用的研究[J].福建中医学院学报,2007,17(3):29-31.
[7]董海燕,董亚琳,贺浪冲,等.白术抗炎活性成分的研究[J].中国药学杂志,2007,42(14):1055-1059.
[8]岳美颖,潘媛,敖慧.白术化学、药理与临床研究进展[J].亚太传统医药,2016,12(5):66-68.
[9]杨娥,钟艳梅,冯毅凡.白术化学成分和药理作用的研究进展[J].广东药学院学报,2012,28(2):218-221.
[10]Gao H,Huang YN,Gao B,et al.Inhibitory effect onα-glucosidase by Adhatoda vasica Nees[J].Food Chem,2008,108(3):965-972.
[11]张连群,周小琴.白术内酯类成分与药理作用的研究进展[J].按摩与康复医学,2017,8(21):1-2.
[12]李伟,文红梅,崔小兵,等.白术健脾有效成分研究[J].南京中医药大学学报:自然科学版,2006,22(6):366-367.
[13]Yoon M,Kim MY.The anti-angiogenic herbal composition Ob-Xfrom Morus alba,Melissa officinalis,and Artemisia capillaris regulates obesity in genetically obese ob/ob mice[J].Pham Biol,2011,49(6):614-619.
[14]林霄.茵陈蒿的药理作用研究[J].长春中医药大学学报,2008,24(6):663.
[15]李铁英,关晓海,邢朝斌,等.青蒿琥酯对糖尿病大鼠视网膜细胞凋亡的作用[J].中国医药,2016,11(4):536-539.
[16]曹锦花.茵陈的化学成分和药理作用研究进展[J].沈阳药科大学学报,2013,30(6):489-494.
[17]王茜.茵陈的药理作用及其主要化学成分药物代谢动力学研究进展[J].安徽中医学院学报,2012,31(4):87-90.
[18]艾力,张奕,吴小海,等.茵陈对神经细胞保护机制的研究现状和进展[J].交通医学,2017,31(2):136-138.
[19]魏建华,刘学敏.茵陈的现代药理研究[J].基层医学论坛,2009,13(22):743-744.
[20]王喜军,李廷利,孙晖.茵陈蒿汤及其血中移行成分6,7-二甲氧基香豆素的肝保护作用[J].中国药理学通报,2004,20(2):239-240.
[21]闫晗,孙媛媛,于治国,等.HPLC法同时测定白术药材中白术内酯Ⅰ等4种有效成分的含量[J].沈阳药科大学学报,2015,32(9):699-702,708.
[22]李羾,何丹,杨常成.高效液相色谱法测定白术中白术内酯Ⅰ、Ⅱ和Ⅲ的含量[J].中南药学,2014,12(1):70-73.
[23]尹华,王知青,王玲,等.HPLC-DAD波长切换法同时测定白术中白术内酯Ⅰ、Ⅱ、Ⅲ和苍术酮的含量[J].中华中医药杂志,2013,28(1):233-236.
[24]王韵,赵亮,柴逸峰,等.RP-HPLC法同时测定茵陈蒿汤中14个成分[J].药物分析杂志,2017,37(3):393-401.
[2]王涵,杨娜,谭静,等.白术化学成分、药理作用及临床应用的研究进展[J].甘肃医药,2018,37(1):23-26.
[3]姜淋洁,付涛,卢锟刚,等.白术提取物对大鼠预防性调血脂及保肝作用的实验研究[J].数理医药学杂志,2011,24(4):398-401.
[4]向小庆,叶红.白术抗肿瘤作用的研究及应用进展[J].中国实验方剂学杂志,2013,19(8):367-370.
[5]李雯,尹华.白术化学成分的药理作用研究进展[J].海峡药学,2012,24(3):9-11.
[6]施文荣,刘艳,陈玲,等.白术燥湿利水作用的研究[J].福建中医学院学报,2007,17(3):29-31.
[7]董海燕,董亚琳,贺浪冲,等.白术抗炎活性成分的研究[J].中国药学杂志,2007,42(14):1055-1059.
[8]岳美颖,潘媛,敖慧.白术化学、药理与临床研究进展[J].亚太传统医药,2016,12(5):66-68.
[9]杨娥,钟艳梅,冯毅凡.白术化学成分和药理作用的研究进展[J].广东药学院学报,2012,28(2):218-221.
[10]Gao H,Huang YN,Gao B,et al.Inhibitory effect onα-glucosidase by Adhatoda vasica Nees[J].Food Chem,2008,108(3):965-972.
[11]张连群,周小琴.白术内酯类成分与药理作用的研究进展[J].按摩与康复医学,2017,8(21):1-2.
[12]李伟,文红梅,崔小兵,等.白术健脾有效成分研究[J].南京中医药大学学报:自然科学版,2006,22(6):366-367.
[13]Yoon M,Kim MY.The anti-angiogenic herbal composition Ob-Xfrom Morus alba,Melissa officinalis,and Artemisia capillaris regulates obesity in genetically obese ob/ob mice[J].Pham Biol,2011,49(6):614-619.
[14]林霄.茵陈蒿的药理作用研究[J].长春中医药大学学报,2008,24(6):663.
[15]李铁英,关晓海,邢朝斌,等.青蒿琥酯对糖尿病大鼠视网膜细胞凋亡的作用[J].中国医药,2016,11(4):536-539.
[16]曹锦花.茵陈的化学成分和药理作用研究进展[J].沈阳药科大学学报,2013,30(6):489-494.
[17]王茜.茵陈的药理作用及其主要化学成分药物代谢动力学研究进展[J].安徽中医学院学报,2012,31(4):87-90.
[18]艾力,张奕,吴小海,等.茵陈对神经细胞保护机制的研究现状和进展[J].交通医学,2017,31(2):136-138.
[19]魏建华,刘学敏.茵陈的现代药理研究[J].基层医学论坛,2009,13(22):743-744.
[20]王喜军,李廷利,孙晖.茵陈蒿汤及其血中移行成分6,7-二甲氧基香豆素的肝保护作用[J].中国药理学通报,2004,20(2):239-240.
[21]闫晗,孙媛媛,于治国,等.HPLC法同时测定白术药材中白术内酯Ⅰ等4种有效成分的含量[J].沈阳药科大学学报,2015,32(9):699-702,708.
[22]李羾,何丹,杨常成.高效液相色谱法测定白术中白术内酯Ⅰ、Ⅱ和Ⅲ的含量[J].中南药学,2014,12(1):70-73.
[23]尹华,王知青,王玲,等.HPLC-DAD波长切换法同时测定白术中白术内酯Ⅰ、Ⅱ、Ⅲ和苍术酮的含量[J].中华中医药杂志,2013,28(1):233-236.
[24]王韵,赵亮,柴逸峰,等.RP-HPLC法同时测定茵陈蒿汤中14个成分[J].药物分析杂志,2017,37(3):393-401.