复合酶法提取化橘红总黄酮工艺研究
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摘要
目的:优选复合酶法提取化橘红总黄酮的工艺条件。方法:在单因素试验的基础上,采用BoxBehnken设计,以总黄酮含量为评价指标,分别考察酶解时间p H值及酶用量对提取效果的影响。结果:最佳提取工艺为酶用量0.32%,p H值为4.83,50℃水浴中振荡酶解0.5 h,滤渣再加入60%乙醇加热回流处理1 h。结论:酶解辅助提取化橘红总黄酮效果理想,建立的数学模型预测性良好。
Objective:To optimize the extraction conditions of total flavonoids from tomentose pummelo by composite enzyme method. Methods:Based on the single factor experiment,the content of total flavonoids as the evaluation index,the Box-Behnken design was used to investigate the effect of the time,p H value and enzyme dosage. Results:The optimum extraction conditions were concluded as follows:composite enzyme amount of 0.33%,p H of 4.83 and enzymolysis 0.5 h at 50 ℃. Then the drug residue was heated with 60% ethanol for 1 h. Conclusion:The mathematical model had good prediction.
Objective:To optimize the extraction conditions of total flavonoids from tomentose pummelo by composite enzyme method. Methods:Based on the single factor experiment,the content of total flavonoids as the evaluation index,the Box-Behnken design was used to investigate the effect of the time,p H value and enzyme dosage. Results:The optimum extraction conditions were concluded as follows:composite enzyme amount of 0.33%,p H of 4.83 and enzymolysis 0.5 h at 50 ℃. Then the drug residue was heated with 60% ethanol for 1 h. Conclusion:The mathematical model had good prediction.
引文
[1]国家药典委员会.中华人民共和国药典[M].北京:化学工业出版社,2015:74.
[2]张超洪,肖维强,赵鹏,等.广东省化橘红中黄酮类物质的HPLC测定[J].华中农业大学学报,2009,28(4):483-486.
[3]陈永刚,刘晓涵,林励,等.超声波提取法化橘红中黄酮类物质的工艺优选[J].中药材,2009,32(1):138-141.
[4]李吉华.化橘红中柚皮苷、柚皮芸香苷、野漆树苷和柚皮素的HPLC测定[J].山东中医杂志,2012,31(4):277-278.
[5]莫小路,蔡岳文,曾庆钱.中药化橘红的研究进展[J].食品与药品,2007,9(6):39-41.
[6]李慕春,阿力甫·阿不都,贺飞,等.戈宝红麻总黄酮酶法提取工艺研究[J].中南药学,2015,13(3):270-273.
[7]彭晓青,程轩轩,张旭红,等.复合酶法提取龙眼核总黄酮的工艺研究[J].中国医院用药评价与分析,2014,14(2):134-136.
[2]张超洪,肖维强,赵鹏,等.广东省化橘红中黄酮类物质的HPLC测定[J].华中农业大学学报,2009,28(4):483-486.
[3]陈永刚,刘晓涵,林励,等.超声波提取法化橘红中黄酮类物质的工艺优选[J].中药材,2009,32(1):138-141.
[4]李吉华.化橘红中柚皮苷、柚皮芸香苷、野漆树苷和柚皮素的HPLC测定[J].山东中医杂志,2012,31(4):277-278.
[5]莫小路,蔡岳文,曾庆钱.中药化橘红的研究进展[J].食品与药品,2007,9(6):39-41.
[6]李慕春,阿力甫·阿不都,贺飞,等.戈宝红麻总黄酮酶法提取工艺研究[J].中南药学,2015,13(3):270-273.
[7]彭晓青,程轩轩,张旭红,等.复合酶法提取龙眼核总黄酮的工艺研究[J].中国医院用药评价与分析,2014,14(2):134-136.