酶解法辅助提取布渣叶总黄酮的工艺优选
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摘要
目的:优选酶解法辅助提取布渣叶总黄酮的工艺条件。方法:以总黄酮提取率为指标,采用单因素试验、正交试验法,分别考察酶种类、酶用量、p H值、酶解时间及乙醇浓度对提取工艺的影响。结果:最佳工艺条件为复合酶(纤维素酶∶果胶酶=1∶1)用量1%,乙醇浓度60%,酶解1h,总黄酮提取率为20. 90%,较未经酶解法提高了4. 85%。结论:优选的工艺简单高效,为布渣叶总黄酮的开发利用提供了实验依据。
Objective:To optimize the process conditions for enzymolysis in assisting the extraction of total flavonoids from Microcos paniculata. Methods:With the extraction rate of total flavonoids as an index,the single-factor experiment or orthogonal test was used to assess the influence of type of enzyme,amount of enzyme used,p H value,time of enzymolysis,and alcohol concentration on extraction process. Results:The optimal process conditions were compound enzyme( cellulose:pectinase = 1∶ 1) with an amount of 1%,an alcohol concentration of 60%,and a duration of enzymolysis of 1 hour. The extraction rate of total flavonoids was 20. 90%,which was 4. 85% higher than the extraction rate obtained by unenzymatic hydrolysis. Conclusion:The optimized process is simple and efficient and provides an experimental basis for the exploitation and utilization of total flavonoids from Microcos paniculata.
Objective:To optimize the process conditions for enzymolysis in assisting the extraction of total flavonoids from Microcos paniculata. Methods:With the extraction rate of total flavonoids as an index,the single-factor experiment or orthogonal test was used to assess the influence of type of enzyme,amount of enzyme used,p H value,time of enzymolysis,and alcohol concentration on extraction process. Results:The optimal process conditions were compound enzyme( cellulose:pectinase = 1∶ 1) with an amount of 1%,an alcohol concentration of 60%,and a duration of enzymolysis of 1 hour. The extraction rate of total flavonoids was 20. 90%,which was 4. 85% higher than the extraction rate obtained by unenzymatic hydrolysis. Conclusion:The optimized process is simple and efficient and provides an experimental basis for the exploitation and utilization of total flavonoids from Microcos paniculata.
引文
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[7]彭晓青,程轩轩,张旭红,等.复合酶法提取龙眼核总黄酮的工艺研究[J].中国医药用药评价与分析,2014,14(2):135-138.
[8]李慕春,阿力浦,阿不都,等.戈宝红麻总黄酮酶法提取工艺研究[J].中南药学,2015,13(3):270-273.
[9]周大寨,朱玉昌,黄卫,等.复合酶法提取板党多糖的研究[J].时珍国医国药,2009,20(8):1928-1930.
[10]曾巧煌,陈玉兴,曾晓会,等.布渣叶总黄酮分散片对不同高血脂症模型调血脂作用研究[J].江西中医药,2016,47(1):63-65.
[2]冯世秀,刘梅芳,魏孝义,等.布渣叶中三萜和黄酮类成分的研究[J].热带亚热带植物报,2008,16(1):51-56.
[3]杨茵,李硕果,叶文才,等.布渣叶的化学成分研究[J].时珍国医国药,2010,21(11):2790-2792.
[4]毕和平,韩长日,王芳,等.分光光度法测定破布叶中总黄酮[J].广东化工,2006,33(3):43-45.
[5]隋晓璠,王超,李永吉,等.均匀设计优化纤维素酶解提取三七工艺的研究[J].中医药学报,2005,33(4):8-12.
[6]高霞,刘聪燕,陈彦,等.酶技术在中药黄酮类成分研究中的应用[J].中草药,2014,45(16):2142-2146.
[7]彭晓青,程轩轩,张旭红,等.复合酶法提取龙眼核总黄酮的工艺研究[J].中国医药用药评价与分析,2014,14(2):135-138.
[8]李慕春,阿力浦,阿不都,等.戈宝红麻总黄酮酶法提取工艺研究[J].中南药学,2015,13(3):270-273.
[9]周大寨,朱玉昌,黄卫,等.复合酶法提取板党多糖的研究[J].时珍国医国药,2009,20(8):1928-1930.
[10]曾巧煌,陈玉兴,曾晓会,等.布渣叶总黄酮分散片对不同高血脂症模型调血脂作用研究[J].江西中医药,2016,47(1):63-65.