戈宝红麻总黄酮酶法提取工艺研究
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摘要
目的优化复合酶酶解辅助水提取戈宝红麻叶总黄酮的小试工艺条件。方法对提取时间、温度、料液比、酶用量、p H值等条件进行单因素考察,根据单因素考察结果设计正交试验,以总黄酮得率为指标进行考察,并进行验证试验。结果优化的提取条件:温度50℃,酶量0.16 m L·g-1,p H值为5.5,料液比为1:20,时间1.5 h,在此条件下总黄酮提取率可达到2.74%。结论酶解辅助提取戈宝红麻叶总黄酮效果较好,应用前景广阔。
Objective To optimize the extraction process of flavone in Apocynum Venetum L. based on compound enzyme. Methods The single factor test was performed to determine the effect of extraction time, material-liquid ratio, extraction temperature, and the concentration of cellulase and p H on the yield rate of flavone from the leaves of Apocynum Venetum L.. The process of extracting flavone based on cellulose was optimized with orthogonal test. Results The optimized extraction condition was: the extraction temperature was 50 ℃, the quantity of cellulase was 0.16 m L·g-1, the p H was 5.5, the material-liquidratio was 1 :20 and the extraction time was 1.5 h. Under this condition, the extraction ratio of flavones reached 2.74%. Conclusion The extraction efficiency of flavones from Apocynum Venetum L. leaves based on cellluase is better.
Objective To optimize the extraction process of flavone in Apocynum Venetum L. based on compound enzyme. Methods The single factor test was performed to determine the effect of extraction time, material-liquid ratio, extraction temperature, and the concentration of cellulase and p H on the yield rate of flavone from the leaves of Apocynum Venetum L.. The process of extracting flavone based on cellulose was optimized with orthogonal test. Results The optimized extraction condition was: the extraction temperature was 50 ℃, the quantity of cellulase was 0.16 m L·g-1, the p H was 5.5, the material-liquidratio was 1 :20 and the extraction time was 1.5 h. Under this condition, the extraction ratio of flavones reached 2.74%. Conclusion The extraction efficiency of flavones from Apocynum Venetum L. leaves based on cellluase is better.
引文
[1]萧正春,刘起棠,张卫明,等.戈宝红麻野生株丛物候学观察[J].中国野生植物资源,2009,28(6):11-14.
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[5]张语迟,刘春明,刘志强,等.戈宝红麻叶黄酮类成分酶解前后的液相色谱-质谱分析及活性比较[J].分析测试学报,2010,29(10):1073-1077.
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[8]韩爱霞,任彦蓉,黄靖,等.戈宝红麻叶总黄酮类化合物的酶解-溶剂提取工艺研究[J].安徽农业科学,2008,36(33):14587-14588.
[9]石森,李惠芬,李新元.工业提取重楼薯蓣皂苷元的工艺研究[J].天津药学,2010,22(5):74-76.
[11]许虎,王媚,刘训红,等.戈宝红麻叶中黄酮类成分的定位与相对定量[J].药学学报,2011,46(8):1004-1007.
[12]贾亚伟,丁玉.纤维素酶辅助提取橙皮苷的工艺研究[J].江苏农业科学,2011,39(4):335-338.
[13]梁靖,须海荣,蒋文莉,等.纤维素酶在速溶茶中的应用研究[J].茶叶,2002,28(1):25-26.
[14]马陶陶,张群林,李俊,等.三氯化铝比色法测定中药总黄酮方法的探讨[J].时珍国医国药,2008,19(1):54-56.
[2]刘起棠,萧正春,张卫明,等.戈宝红麻茶品质初探[J].中国野生植物资源,2010,29(1):18-20.
[3]曾海松.戈宝红麻叶的化学成分研究[D].西安:西北大学,2009.
[4]虞颖映.戈宝红麻茶对心血管系统的生物学效应及其安全性评价[D].杭州:浙江大学,2006.
[5]张语迟,刘春明,刘志强,等.戈宝红麻叶黄酮类成分酶解前后的液相色谱-质谱分析及活性比较[J].分析测试学报,2010,29(10):1073-1077.
[6]马挺军,刘诚,李久长,等.大花戈宝红麻叶黄酮的提取[J].食品科学,2005,26(12):273-274.
[7]赵明,王宁.大叶白麻叶总黄酮超声波提取工艺研究[J].西北农业学报,2007,16(4):269-271.
[8]韩爱霞,任彦蓉,黄靖,等.戈宝红麻叶总黄酮类化合物的酶解-溶剂提取工艺研究[J].安徽农业科学,2008,36(33):14587-14588.
[9]石森,李惠芬,李新元.工业提取重楼薯蓣皂苷元的工艺研究[J].天津药学,2010,22(5):74-76.
[11]许虎,王媚,刘训红,等.戈宝红麻叶中黄酮类成分的定位与相对定量[J].药学学报,2011,46(8):1004-1007.
[12]贾亚伟,丁玉.纤维素酶辅助提取橙皮苷的工艺研究[J].江苏农业科学,2011,39(4):335-338.
[13]梁靖,须海荣,蒋文莉,等.纤维素酶在速溶茶中的应用研究[J].茶叶,2002,28(1):25-26.
[14]马陶陶,张群林,李俊,等.三氯化铝比色法测定中药总黄酮方法的探讨[J].时珍国医国药,2008,19(1):54-56.