宝鸡市不同小麦品种水培叶片抗性指标差异分析
|
摘要
目的了解宝鸡市不同小麦品种的抗逆性,筛选该区适合种植的小麦品种。方法选取11个冬小麦品种的种子为研究对象,以丙二醛(MDA)含量、过氧化物酶(POD)活性和超氧化物歧化酶(SOD)活性为抗逆性指标进行分析,最后对这几个指标进行相关分析。结果 (1)不同品种小麦叶片MDA含量差异显著(F=32.29,P<0.01),以宝研85叶片的MDA含量最高,为22.95 mmol·g~(-1)FW,以周麦27和荔高2号MDA含量较低,分别为4.91和4.86mmol·g~(-1) FW;(2)不同品种小麦叶片过氧化物酶(POD)活性之间达到极显著差异(F=77.78,P<0.01),其中,周麦27叶片的POD活性最强,为32.67U/min·g FW,宝研85叶片的POD活性最弱,为11.53U/min·g FW;(3)不同品种小麦叶片超氧化物歧化酶(SOD)活性也达到极显著差异(F=15.83,P<0.01),以周麦27叶片的超氧化物歧化酶活性最强,其值为43.76U/g FW,活性值最低的品种是宝研85,其值为39.21U/g FW;(4)不同品种间MDA含量与POD及SOD活性均呈现显著的负相关关系,相关系数分别为-0.75和-0.83,POD与SOD呈现显著的正相关关系,相关系数为0.68。结论宝鸡市主栽的11个小麦品种以周麦27的抗性最强,宝研85抗性较弱。
Purposes—To understand the stress resistance of different wheat varieties in Baoji City,Shaanxi,China and to screen the wheat varieties suitable for being grown in the area.Methods—The seeds of 11 winter wheat varieties in Baoji City were selected as the research objects and were analyzed with the malondialdehyde(MDA)content as well as peroxidase(POD)and superoxide dismutase(SOD)as the indices of resistance,then the relationships among these three indices were also investigated.Results—(1)There was more significant difference in the leaf MDA content among different wheat varieties(F=32.99,P<0.01),the highest MDA content,22.95mmol·g~(-1) FW,was in Baoyan 85;the lowest MDA content was in Zhoumai 27 and Ligao 2,their value was 4.91 and 4.86mmol·g~(-1) FW,respectively;(2)There was more significant difference in leaf POD activities among different wheat varieties(F=77.78,P<0.01).The strongest POD activity,32.67U/min·g FW,was in Zhoumai 27;the lowest value of activity,11.53U/min·g FW,was in Baoyan 85;(3)There was also more significant differences in the SOD activity(F=15.83,P<0.01)among 11 wheat varieties,and the Zhoumai 27 had the highest superoxide dismutase activity,43.76 U/g FW,while the lowest activity,39.21U/g FW,was in Baoyan 85;(4)There were significant negative correlations between MDA content and POD,SOD activities in different variety seeds,and the correlation coefficient was-0.75and-0.83,separately,while the relationship between POD and SOD was positive significant and the correlation coefficient was 0.68.Conclusions—Among the 11 main wheat varieties in Baoji City,Zhoumai 27 has the strongest resistance while the resistance of Baoyan 85 is the weakest.
Purposes—To understand the stress resistance of different wheat varieties in Baoji City,Shaanxi,China and to screen the wheat varieties suitable for being grown in the area.Methods—The seeds of 11 winter wheat varieties in Baoji City were selected as the research objects and were analyzed with the malondialdehyde(MDA)content as well as peroxidase(POD)and superoxide dismutase(SOD)as the indices of resistance,then the relationships among these three indices were also investigated.Results—(1)There was more significant difference in the leaf MDA content among different wheat varieties(F=32.99,P<0.01),the highest MDA content,22.95mmol·g~(-1) FW,was in Baoyan 85;the lowest MDA content was in Zhoumai 27 and Ligao 2,their value was 4.91 and 4.86mmol·g~(-1) FW,respectively;(2)There was more significant difference in leaf POD activities among different wheat varieties(F=77.78,P<0.01).The strongest POD activity,32.67U/min·g FW,was in Zhoumai 27;the lowest value of activity,11.53U/min·g FW,was in Baoyan 85;(3)There was also more significant differences in the SOD activity(F=15.83,P<0.01)among 11 wheat varieties,and the Zhoumai 27 had the highest superoxide dismutase activity,43.76 U/g FW,while the lowest activity,39.21U/g FW,was in Baoyan 85;(4)There were significant negative correlations between MDA content and POD,SOD activities in different variety seeds,and the correlation coefficient was-0.75and-0.83,separately,while the relationship between POD and SOD was positive significant and the correlation coefficient was 0.68.Conclusions—Among the 11 main wheat varieties in Baoji City,Zhoumai 27 has the strongest resistance while the resistance of Baoyan 85 is the weakest.
引文
[1]寇秀云.不同品种小麦苗营养功能成分与抗氧化能力比较研究[D].杨凌:西北农林科技大学,2016.
[2]徐月明.密肥调控对中、弱筋小麦生育后期旗叶生理特性的影响[J].山东农业科学,2009(11):21-25.
[3]ZHANG B B,CHANG S X,ANYIA A O.Mycorrhizal inoculation and nitrogen fertilization affect the physiology and growth of spring wheat under two contrasting water regimes[J].Plant and Soil,2016,398(1/2),47-57.DOI:10.1007/s11104-015-2635-x.
[4]ZHANG B B,LIU W Z,CHANG S X,et al.Water-deficit and high temperature affected water use efficiency and arabinoxylan concentration in spring wheat[J].Journal of Cereal Science,2010,52(2),263-269.DOI:10.1016/j.jcs.2010.05.014.
[5]任雅琴,吕金仓,张慧成,等.宝鸡市2015-2016年度小麦减产原因浅析[J].农业科技通讯,2017(3):210-212.
[6]刘瑞,侯江龙,陈洁,等.宝鸡市小麦-玉米一体化施肥试验研究[J].陕西农业科学,2016,62(7):3-5.
[7]宋佃星,马莉.宝鸡市气候变化及其对小麦产量的影响[J].中国农学通报,2016,32(19):147-151.
[8]强红妮,封涌涛,李翊阁.宝鸡市小麦施肥配方研究[J].陕西农业科学,2015,61(1):57-59.
[9]孙蕊,林奇,王晓楠,等.低温下寒地冬小麦抗氧化生理指标的比较分析[J].中国农学通报,2015,31(12):1-5.
[10]吴娟娟.细胞分裂素对小麦幼苗生长相关酶活性的影响[J].湖北农业科学,2011,50(24):5044-5045.
[11]赵会杰,薛延丰.外源水杨酸对光抑制条件下小麦叶片光合作用的影响[J].植物生理学通讯,2005,41(5):613-615.
[12]彭艳,李洋,杨广笑,等.铝胁迫对不同小麦SOD、CAT、POD活性和MDA含量的影响[J].生物技术,2006,16(3):38-42.
[13]成利娟,苏涌,杨翠,等.小麦胚芽油体内外抗氧化作用的研究[J].安徽医药,2013,17(8):1289-1291.
[14]徐心诚.低温弱光对黄瓜幼苗过氧化物酶同工酶和丙二醛含量的影响[J].河南农业科学,2012,41(1):113-116.
[15]冯佰利,高小丽,王长发,等.干旱条件下不同温型小麦叶片衰老与活性氧代谢特性的研究[J].中国生态农业学报,2005,13(4):74-76.
[16]杨文玲,巩涛,刘莹莹,等.铅铬胁迫对小麦幼苗抗氧化酶活性和丙二醛含量的影响[J].中国农学通报,2015,31(6):45-50.
[17]SUGAMURA K,KEANEY J F.Reactive oxygen species in cardiovascular disease[J].Free Radical Biology and Medicine,2011,51(5):978-992.DOI:10.1016/j.freeradbiomed.2011.05.004.
[18]党芳芳,冯文新,尹美强,等.硅钙肥与纳米碳对干旱胁迫下小麦幼苗形态及生理的影响[J].山西农业大学学报(自然科学版),2017,37(3):164-168.
[19]吴美金,王敏,张从宇.赤霉菌粗毒素胁迫对小麦幼苗生理指标的影响[J].中国农学通报,2010,26(23):168-172.
[20]周萍,庄丽,徐红军,等.水肥运筹对滴灌春小麦保护酶活性及产量的影响[J].麦类作物学报,2013,33(3):561-565.
[2]徐月明.密肥调控对中、弱筋小麦生育后期旗叶生理特性的影响[J].山东农业科学,2009(11):21-25.
[3]ZHANG B B,CHANG S X,ANYIA A O.Mycorrhizal inoculation and nitrogen fertilization affect the physiology and growth of spring wheat under two contrasting water regimes[J].Plant and Soil,2016,398(1/2),47-57.DOI:10.1007/s11104-015-2635-x.
[4]ZHANG B B,LIU W Z,CHANG S X,et al.Water-deficit and high temperature affected water use efficiency and arabinoxylan concentration in spring wheat[J].Journal of Cereal Science,2010,52(2),263-269.DOI:10.1016/j.jcs.2010.05.014.
[5]任雅琴,吕金仓,张慧成,等.宝鸡市2015-2016年度小麦减产原因浅析[J].农业科技通讯,2017(3):210-212.
[6]刘瑞,侯江龙,陈洁,等.宝鸡市小麦-玉米一体化施肥试验研究[J].陕西农业科学,2016,62(7):3-5.
[7]宋佃星,马莉.宝鸡市气候变化及其对小麦产量的影响[J].中国农学通报,2016,32(19):147-151.
[8]强红妮,封涌涛,李翊阁.宝鸡市小麦施肥配方研究[J].陕西农业科学,2015,61(1):57-59.
[9]孙蕊,林奇,王晓楠,等.低温下寒地冬小麦抗氧化生理指标的比较分析[J].中国农学通报,2015,31(12):1-5.
[10]吴娟娟.细胞分裂素对小麦幼苗生长相关酶活性的影响[J].湖北农业科学,2011,50(24):5044-5045.
[11]赵会杰,薛延丰.外源水杨酸对光抑制条件下小麦叶片光合作用的影响[J].植物生理学通讯,2005,41(5):613-615.
[12]彭艳,李洋,杨广笑,等.铝胁迫对不同小麦SOD、CAT、POD活性和MDA含量的影响[J].生物技术,2006,16(3):38-42.
[13]成利娟,苏涌,杨翠,等.小麦胚芽油体内外抗氧化作用的研究[J].安徽医药,2013,17(8):1289-1291.
[14]徐心诚.低温弱光对黄瓜幼苗过氧化物酶同工酶和丙二醛含量的影响[J].河南农业科学,2012,41(1):113-116.
[15]冯佰利,高小丽,王长发,等.干旱条件下不同温型小麦叶片衰老与活性氧代谢特性的研究[J].中国生态农业学报,2005,13(4):74-76.
[16]杨文玲,巩涛,刘莹莹,等.铅铬胁迫对小麦幼苗抗氧化酶活性和丙二醛含量的影响[J].中国农学通报,2015,31(6):45-50.
[17]SUGAMURA K,KEANEY J F.Reactive oxygen species in cardiovascular disease[J].Free Radical Biology and Medicine,2011,51(5):978-992.DOI:10.1016/j.freeradbiomed.2011.05.004.
[18]党芳芳,冯文新,尹美强,等.硅钙肥与纳米碳对干旱胁迫下小麦幼苗形态及生理的影响[J].山西农业大学学报(自然科学版),2017,37(3):164-168.
[19]吴美金,王敏,张从宇.赤霉菌粗毒素胁迫对小麦幼苗生理指标的影响[J].中国农学通报,2010,26(23):168-172.
[20]周萍,庄丽,徐红军,等.水肥运筹对滴灌春小麦保护酶活性及产量的影响[J].麦类作物学报,2013,33(3):561-565.