小麦胚芽油体内外抗氧化作用的研究
|
摘要
目的研究小麦胚芽油的体外自由基清除率及影响大鼠体内超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的酶比活力及丙二醛(MDA)含量的影响。方法采用α,α-二苯-β-苦味肼(DPPH)法、[2,2-连氮-(3-乙基苯并噻唑啉-6-磺酸)二铵盐](ABTS)法计算自由基清除率(Inhibition percent IP);比色法测定大鼠肝组织及血清SOD、GSH-Px酶比活力及MDA含量。结果小麦胚芽油清除自由基能力与其浓度呈明显的量效(正比)关系。与空白对照组比较,中、高剂量组SOD、GSH-Px酶比活力显著提高,MDA含量降低,差异具有统计学意义(P<0.05)。结论小麦胚芽油具有明显的体内外抗氧化作用。
Objective To study the pharmacodynamics function of wheat germ oil on the clearance rate of free radicals in vivo and the influence on superoxide dismutase(SOD),glutathione-peroxidase(GSH-px) activity and malondialdehyde(MDA) content in vitro of rats.Methods We calculated the inhibition percent(IP) of free radical with DPPH and ABTS methods,detected SOD,GSH-Px specific activity and MDA content in liver homogenate and serum by colorimetric method.Results With the wheat germ oil,the free radical-scavenging rose in a dose-dependent manner.Compared with those of the blank control group,SOD,GSH-Px specific activity of medium and high dose groups were markedly enhanced while the content of MDA were reduced significantly and the differences had statistical significance(P < 0.05).Conclusion There is an antioxidative effect of wheat germ oil in vitro and vivo obviously.
Objective To study the pharmacodynamics function of wheat germ oil on the clearance rate of free radicals in vivo and the influence on superoxide dismutase(SOD),glutathione-peroxidase(GSH-px) activity and malondialdehyde(MDA) content in vitro of rats.Methods We calculated the inhibition percent(IP) of free radical with DPPH and ABTS methods,detected SOD,GSH-Px specific activity and MDA content in liver homogenate and serum by colorimetric method.Results With the wheat germ oil,the free radical-scavenging rose in a dose-dependent manner.Compared with those of the blank control group,SOD,GSH-Px specific activity of medium and high dose groups were markedly enhanced while the content of MDA were reduced significantly and the differences had statistical significance(P < 0.05).Conclusion There is an antioxidative effect of wheat germ oil in vitro and vivo obviously.
引文
[1]孙光艳.小麦胚芽油提取方法的比较分析[J].科技创新与应用,2012(2):10.
[2]陶艳艳,王清兰,袁继丽,等.维生素E抑制氯化汞诱导大鼠肾间质纤维化的抗氧化作用机制[J].中西医结合学报,2011,9(2):201-208.
[3]严晓月,吴金鸿,钟耀广,等.小麦胚芽综合开发利用研究进展[J].农产品加工学刊,2012(11):14-18.
[4]Jiang ST,Niu LY.Optimization and evaluation of wheat germ oil ex-tracted by supercritical CO2[J].Grass Y Aceites,2011,62(2):181-189.
[5]Karabacak M,Kanbur M,Eraslan G,et al.The antioxidant effect ofwheat germ oil on subchronic coumaphos exposure in mice[J].Eco-toxicol Environ Saf,2011,74(7):2119-2125.
[6]Krings U,Yasser S,Bader A,et al.Antioxidant activity of extractsfrom roasted wheat germ.[J].Food Chemistry,2000(71):91-95.
[7]Wang JG,Wang YT,Liu XB,et al.Free radical scavenging andim-munomodulatory activities of Ganoderma lucidum polysaccharidesderivatives[J].Carbohydr Polym,2013,91(1):33-38.
[8]杨启纲,罗晓明,张琼.乌司他丁治疗重症急性胰腺炎的临床疗效观察[J].安徽医药,2011,15(9):1147-1148.
[9]徐佳杨,蔡辉.氧化低密度脂蛋白与动脉粥样硬化的研究[J].安徽医药,2011,15(2):228-230.
[10]Sugamura K,Keaney JF.Reactive oxygen species in cardiovasculardisease[J].Free Radical Biology and Medicine,2011,51(5):978-992.
[11]Alessandri C,Pignatelli P,Loffredo L,et al.Alpha-Linolenic Acid-Rich Wheat Germ Oil Decreases Oxidative Stress and CD40 Ligandin Patients With Mild Hypercholesterolemia[J].Arterioscler ThrombVasc Biol,2006,26(11):2577-2588.
[2]陶艳艳,王清兰,袁继丽,等.维生素E抑制氯化汞诱导大鼠肾间质纤维化的抗氧化作用机制[J].中西医结合学报,2011,9(2):201-208.
[3]严晓月,吴金鸿,钟耀广,等.小麦胚芽综合开发利用研究进展[J].农产品加工学刊,2012(11):14-18.
[4]Jiang ST,Niu LY.Optimization and evaluation of wheat germ oil ex-tracted by supercritical CO2[J].Grass Y Aceites,2011,62(2):181-189.
[5]Karabacak M,Kanbur M,Eraslan G,et al.The antioxidant effect ofwheat germ oil on subchronic coumaphos exposure in mice[J].Eco-toxicol Environ Saf,2011,74(7):2119-2125.
[6]Krings U,Yasser S,Bader A,et al.Antioxidant activity of extractsfrom roasted wheat germ.[J].Food Chemistry,2000(71):91-95.
[7]Wang JG,Wang YT,Liu XB,et al.Free radical scavenging andim-munomodulatory activities of Ganoderma lucidum polysaccharidesderivatives[J].Carbohydr Polym,2013,91(1):33-38.
[8]杨启纲,罗晓明,张琼.乌司他丁治疗重症急性胰腺炎的临床疗效观察[J].安徽医药,2011,15(9):1147-1148.
[9]徐佳杨,蔡辉.氧化低密度脂蛋白与动脉粥样硬化的研究[J].安徽医药,2011,15(2):228-230.
[10]Sugamura K,Keaney JF.Reactive oxygen species in cardiovasculardisease[J].Free Radical Biology and Medicine,2011,51(5):978-992.
[11]Alessandri C,Pignatelli P,Loffredo L,et al.Alpha-Linolenic Acid-Rich Wheat Germ Oil Decreases Oxidative Stress and CD40 Ligandin Patients With Mild Hypercholesterolemia[J].Arterioscler ThrombVasc Biol,2006,26(11):2577-2588.