秦岭产宜昌蛇菰生药学特征与抗氧化活性研究
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摘要
目的对秦岭产宜昌蛇菰的生药学特征进行鉴别研究,为品种鉴定提供参考依据;对宜昌蛇菰体外抗氧化活性进行研究,为有效利用其资源提供参考依据。方法对原植物形态、性状、根茎、鳞叶、横切面显微进行鉴别研究;借助紫外分光光度法,通过比较1,1-二苯基-2-三硝基苯肼(DPPH)清除率,评价宜昌蛇菰各部位不同溶剂提取物的体外抗氧化活性。结果宜昌蛇菰的茎横切面表皮以内为薄壁组织,外韧性维管束散在,无明显皮层、中柱以及髓部分化;鳞叶横切面可见主脉维管束散在,无栅栏组织、海绵组织的分化,呈现等面叶的特征。宜昌蛇菰各部位不同溶剂提取液在实验质量浓度范围内,各提取物抗氧化能力均随其质量浓度升高而增强。结论宜昌蛇菰花茎和鳞叶横切面初生构造的特征,对其品种鉴定提供参考依据。宜昌蛇菰根茎、鳞叶、花均具有明显抗氧化活性,三者间无明显差异,故提示其应全株入药。
Objective To identify the pharmacognostical characteristics of Qinling Balanophora henryi to provide a reference for species identification,and to study the antioxidation ability of Balanophora henryi so as to provide references for its efficient use. Methods We observed the morphology,properties,rhizome,squamous leaves and the cross-sectionmicrostructure of the original plant,and compared the DPPH clearance rates with UV spectrophotometry to evaluate the extracts of each part in different solvents of Balanophora henryi. Results From the cross-sectional view,basic parenchyma was within the stem,the outer vascular bundles were randomly distributed,and there was no obvious difference in cortex,column and medullary. Squamous leaf cross-section showed the main vein vascular bundles scattered. There was no obvious differentiation in palisade tissue and spongy tissue. Isobilateral leaf characteristics could be seen. Different solvent extracts from various parts were within the experimental concentration range. Antioxidant capacity increased with the increasing concentration of extracts. Conclusion Primary structure characteristics of cross-section of stem and leaf can provide reference for the identification of Balanophora henryi,the stem,leaves and flowers of which have significant antioxidant activity. There were no significant differences among the three parts,which suggested that the whole plant could be used as medicine.
Objective To identify the pharmacognostical characteristics of Qinling Balanophora henryi to provide a reference for species identification,and to study the antioxidation ability of Balanophora henryi so as to provide references for its efficient use. Methods We observed the morphology,properties,rhizome,squamous leaves and the cross-sectionmicrostructure of the original plant,and compared the DPPH clearance rates with UV spectrophotometry to evaluate the extracts of each part in different solvents of Balanophora henryi. Results From the cross-sectional view,basic parenchyma was within the stem,the outer vascular bundles were randomly distributed,and there was no obvious difference in cortex,column and medullary. Squamous leaf cross-section showed the main vein vascular bundles scattered. There was no obvious differentiation in palisade tissue and spongy tissue. Isobilateral leaf characteristics could be seen. Different solvent extracts from various parts were within the experimental concentration range. Antioxidant capacity increased with the increasing concentration of extracts. Conclusion Primary structure characteristics of cross-section of stem and leaf can provide reference for the identification of Balanophora henryi,the stem,leaves and flowers of which have significant antioxidant activity. There were no significant differences among the three parts,which suggested that the whole plant could be used as medicine.
引文
[1]米长忠,周卫华,彭英福,等.湘西产筒鞘蛇菰生态环境及寄主植物初探[J].湖南中医药大学学报,2011,31(11):38-39.
[2]陈吉炎,李聪,王雪芹,等.蛇菰属药用植物的种类与研究进展[J].时珍国医国药,2010,21(8):2032-2034.
[3]陈吉炎,李聪,张路路,等.疏花蛇菰的生药鉴别[J].中药材,2010,33(9):1395-1397.
[4]周卫华,石慧娟,杨梅松竹,等.蛇菰水提物对大鼠酒精性肝损伤的保护作用[J].中国老年学杂志,2012,10(32):4438-4440.
[5]覃大保,米长忠,杨力,等.筒鞘蛇菰壮阳作用的研究[J].中国民族医药杂志,2014,4(4):45-46.
[6]侯钦云,戴忠,程显隆,等.5中蛇菰的化学成分比较研究[J].药物分析杂志,2009,29(5):697-701.
[7]陆应彩,彭霞,台海川,等.傣药思茅蛇菰薄层色谱鉴别[J].中国民族医药杂志,2011,11(11):38.
[8]何玲,高辉,李春艳.筒鞘蛇菰多糖抗疲劳作用研究[J].怀化学院学报,2011,30(11):43-45.
[9]侯文斌,周丽丽,周卫华.蛇菰水提物对自然衰老小鼠的抗衰老作用研究[J].中国民族民间医药,2012,21(3):33.
[10]王慧,张红岐,邬昊洋,等.筒鞘蛇菰提取物及松柏苷的抗氧化作用研究[J].三峡大学学报(自然科学版),2009,31(3):99-101.
[11]刘长龙,雷慧,赵其秀,等.杨树芽提取物清除DPPH自由基的作用[J].安徽医药,2013,17(7):1091-1093.
[12]杨黎江,路金荣,王晓娟,等.蛇菰多糖的提取及测定[J].昆明学院学报,2010,32(3):44-46.
[13]邓靖,莫正昌,汲广全,等.蛇菰提取物体外抗氧化活性研究[J].食品科学,2010,31(5):23-25.
[14]成利娟,苏涌,杨翠,等.小麦胚芽油体内外抗氧化作用的研究[J].安徽医药,2013,17(8):1289-1291.
[2]陈吉炎,李聪,王雪芹,等.蛇菰属药用植物的种类与研究进展[J].时珍国医国药,2010,21(8):2032-2034.
[3]陈吉炎,李聪,张路路,等.疏花蛇菰的生药鉴别[J].中药材,2010,33(9):1395-1397.
[4]周卫华,石慧娟,杨梅松竹,等.蛇菰水提物对大鼠酒精性肝损伤的保护作用[J].中国老年学杂志,2012,10(32):4438-4440.
[5]覃大保,米长忠,杨力,等.筒鞘蛇菰壮阳作用的研究[J].中国民族医药杂志,2014,4(4):45-46.
[6]侯钦云,戴忠,程显隆,等.5中蛇菰的化学成分比较研究[J].药物分析杂志,2009,29(5):697-701.
[7]陆应彩,彭霞,台海川,等.傣药思茅蛇菰薄层色谱鉴别[J].中国民族医药杂志,2011,11(11):38.
[8]何玲,高辉,李春艳.筒鞘蛇菰多糖抗疲劳作用研究[J].怀化学院学报,2011,30(11):43-45.
[9]侯文斌,周丽丽,周卫华.蛇菰水提物对自然衰老小鼠的抗衰老作用研究[J].中国民族民间医药,2012,21(3):33.
[10]王慧,张红岐,邬昊洋,等.筒鞘蛇菰提取物及松柏苷的抗氧化作用研究[J].三峡大学学报(自然科学版),2009,31(3):99-101.
[11]刘长龙,雷慧,赵其秀,等.杨树芽提取物清除DPPH自由基的作用[J].安徽医药,2013,17(7):1091-1093.
[12]杨黎江,路金荣,王晓娟,等.蛇菰多糖的提取及测定[J].昆明学院学报,2010,32(3):44-46.
[13]邓靖,莫正昌,汲广全,等.蛇菰提取物体外抗氧化活性研究[J].食品科学,2010,31(5):23-25.
[14]成利娟,苏涌,杨翠,等.小麦胚芽油体内外抗氧化作用的研究[J].安徽医药,2013,17(8):1289-1291.