小麦胚芽油抗动脉粥样硬化的药效学研究
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摘要
目的观察小麦胚芽油(wheat germ oil,WGO)对动脉粥样硬化(atherosclerosis,AS)大鼠的治疗效果,并探讨其抗AS的可能作用机制。方法连续高脂乳剂灌胃4周,制作AS大鼠模型,分别给予各组大鼠连续灌胃不同剂量WGO 8周;HE染色光镜下观察大鼠主动脉弓病理形态的改变;酶联免疫吸附试验(ELISA)法检测各组大鼠血清中前列环素(Prostacyclin 2,PGI2)、血栓素A2(Thromboxane 2,TXA2)及内皮素-1(Endothelin-1,ET-1)的含量。结果 2.0 g·kg-1的WGO能明显改善AS大鼠主动脉弓的病理状态,基本恢复正常状态;与模型组比较,1.0、2.0 g·kg-1WGO均可显著提高AS大鼠血清中PGI2含量,明显降低TXA2、ET-1水平(P<0.05)。结论 WGO能明显改善AS大鼠的动脉粥样硬化状态。其作用机制可能为小麦胚芽油具有良好的调节PGI2、TXA2平衡,抑制血栓形成,修复内皮损伤以及较强的抗氧化作用。
Objective To investigate the effects of wheat germ oil on atherosclerosis in rats and to explore its possible mechanism.Methods Model of AS rats was made by intragastric administration of fat emulsion for four weeks consecutively. Different doses of WGO were given to the rats which were divided into different groups by intragastric administration for 8 weeks. Pathomorphological changes in aortic arch of AS rats were observed by light microscopy( HE staining). Enzyme- Linked Immunosorbent Assay was used to evaluate the sample concentrations of PGI2,TXA2 and ET-1 in rats' serum. Results The pathomorphological abnormalities of AS rats' aortic arch were significantly improved in high dose group of wheat germ oil,which returned to normal state; WGO could also apparently increase PGI2 and reduced TXA2,ET-1 levels( P < 0. 05). Conclusions Wheat germ oil can significantly improve the state of atherosclerosis in rats. The mechanism may refer to its good ability to regulate the balance between PGI2 and TXA2,inhibit thrombosis and endothelial damage as well as its strong antioxidant effect.
Objective To investigate the effects of wheat germ oil on atherosclerosis in rats and to explore its possible mechanism.Methods Model of AS rats was made by intragastric administration of fat emulsion for four weeks consecutively. Different doses of WGO were given to the rats which were divided into different groups by intragastric administration for 8 weeks. Pathomorphological changes in aortic arch of AS rats were observed by light microscopy( HE staining). Enzyme- Linked Immunosorbent Assay was used to evaluate the sample concentrations of PGI2,TXA2 and ET-1 in rats' serum. Results The pathomorphological abnormalities of AS rats' aortic arch were significantly improved in high dose group of wheat germ oil,which returned to normal state; WGO could also apparently increase PGI2 and reduced TXA2,ET-1 levels( P < 0. 05). Conclusions Wheat germ oil can significantly improve the state of atherosclerosis in rats. The mechanism may refer to its good ability to regulate the balance between PGI2 and TXA2,inhibit thrombosis and endothelial damage as well as its strong antioxidant effect.
引文
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[2]孙光艳.小麦胚芽油提取方法的比较分析[J].科技创新与应用,2012,1(2):10.
[3]Zhao LY,Huang W,Yuan QX,et al.Hypolipidaemic effects and mechanisms of the main component of Opuntia dillenii Haw.polysaccharides in high-fat emulsion-induced hyperlipidaemic rats[J].Food Chemistry,2012,134(2):964-971.
[4]Karabacak M,Kanbur M,Eraslan G,et al.The antioxidant effect of wheat germ oil on subchronic coumaphos exposure in mice[J].Ecotoxicology and Environmental Safety,2011,74(7):2119-2125.
[5]Rashidi B,Mohammadi M,Mirzaei F,et al.Amlodipine treatment decreases plasma and carotid artery tissue levels of endothelin-1in atherosclerotic rabbits[J].Pathophysiology,2011,18(2):137-142.
[6]Van Diepen JA,Berbée JF,Havekes LM,et al.Interactions between inflammation and lipid metabolism:relevance for efficacy of anti-inflammatory drugs in the treatment of atherosclerosis[J].Atherosclerosis,2013,228(2):306-315.
[7]Thomas DD,Ridnour LA,Isenberg JS,et al.The chemical biology of nitric oxide:implications in cellular signaling[J].Free Radical Biology and Medicine,2008,45(1):18-31.
[8]Siasos G,Tousoulis D,Tsigkou V,et al.Flavonoids in atherosclerosis:an overview of their mechanisms of action[J].Current Medicinal Chemistry,2013,20(21):2641-2660.
[9]Fang W,Wei J,Han D,et al.MC-002 exhibits positive effects against platelets aggregation and endothelial dysfunction through thromboxane A2 inhibition[J].Thrombosis Research,2014,133(4):610-615.
[10]Xiao CY,Hara A,Yuhki K,et al.Roles of prostaglandin I2 and thromboxane A2 in cardiac ischemia-reperfusion injury A study using mice lacking their respective receptors[J].Circulation,2001,104(18):2210-2215.
[11]Ivey ME,Osman N,Little PJ.Endothelin-1 signalling in vascular smooth muscle:pathways controlling cellular functions associated with atherosclerosis[J].Atherosclerosis,2008,199(2):237-247.
[12]Mooney D,Mc Carthy C,Belton O.Effects of conjugated linoleic acid isomers on monocyte,macrophage and foam cell phenotype in atherosclerosis[J].Prostaglandins&Other Lipid Mediators,2012,98(3):56-62.
[13]Prim CR,Baroncini LA,Précoma LB,et al.Effects of linseed consumption for a short period of time on lipid profile and atherosclerotic lesions in rabbits fed a hypercholesterolaemicdiet[J].British Journal of Nutrition,2012,107(5):660-664.