针刺肝俞、太冲穴对高尿酸血症大鼠模型尿酸生成的影响及机制研究
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摘要
目的:探究针刺肝俞、太冲穴对高尿酸血症(HUA)大鼠模型尿酸生成相关代谢酶活性的影响,阐释针刺肝俞、太冲穴防治HUA的可能机制。方法:将32只Wistar雄性大鼠随机分成4组,空白组、模型组、肾俞组、肝俞组,每组8只。用氧嗪酸钾灌胃构建HUA大鼠模型。肾俞组和肝俞组分别给予肾俞、太溪穴和肝俞、太冲穴针刺干预。采用生化分析仪检测大鼠SUA的含量,比色法观察大鼠肝匀浆中XOD、ADA的活性,IHC检测XOD阳性相对表达情况,ELISA检测GD的浓度。结果:与模型组比较,肾俞组和肝俞组XOD、ADA的活性降低(P <0. 01)及XOD阳性相对表达水平显著降低(P <0. 01),肝俞组GD的浓度降低(P <0. 01)。与肾俞组比较,肝俞组XOD阳性相对表达水平降低(P <0. 05)。结论:针刺肾俞、太溪穴和肝俞、太冲穴都能降低HUA大鼠XOD、ADA的活性,且针刺肝俞、太冲穴抑制HUA大鼠XOD的效果更明显,还能降低GD的活性,这可能与抑制了嘌呤分解代谢途径,从而减少了尿酸的生成有关。
Objective: To explore the effect and mechanism of needling Ganshu( BL18) and Taichong( LR3)on the activities of metabolic enzymes related to uricopoiesis in the rats with hyperuricemia( HUA),thus to elaborate the possible mechanism of needling BL18 and LR3 in the prevention of HUA. Methods: 32 Wistar male rats were randomly divided into the blank group( n = 8),the model group( n = 8),the Shenshu group( n = 8),and the Ganshu group( n = 8). The HUA models were established by the gavage administration of Oteracil Potassium. The Shenshu group was intervened with needling Shenshu( BL23) and Taixi( LI3); whereas the Ganshu group was intervened with needling BL18 and LR3. The content of serum uric acid( SUA),the activities of XOD and ADA,the positive expression of XOD,and GD concentration were observed in the rats of each group after the treatment. Results: Compared to those in the model group,the activities of XOD and ADA were significantly decreased in the Shenshu group and in the Ganshu group( P < 0. 01); the positive expression of XOD was also significantly decreased in these two groups( P < 0. 01); GD concentration was lowered in the Ganshu group( P < 0. 01). Compared to that in the Shenshu group,the positive expression of XOD was significantly lower in the Ganshu group( P < 0. 05). Conclusion: Acupuncture intervention can reduce SUA level in HUA rats,and its mechanism is related to the metabolic enzymes XOD,ADA and GD,which may inhibit the metabolic pathway of purine decomposition and reduce the generation of uric acid.
Objective: To explore the effect and mechanism of needling Ganshu( BL18) and Taichong( LR3)on the activities of metabolic enzymes related to uricopoiesis in the rats with hyperuricemia( HUA),thus to elaborate the possible mechanism of needling BL18 and LR3 in the prevention of HUA. Methods: 32 Wistar male rats were randomly divided into the blank group( n = 8),the model group( n = 8),the Shenshu group( n = 8),and the Ganshu group( n = 8). The HUA models were established by the gavage administration of Oteracil Potassium. The Shenshu group was intervened with needling Shenshu( BL23) and Taixi( LI3); whereas the Ganshu group was intervened with needling BL18 and LR3. The content of serum uric acid( SUA),the activities of XOD and ADA,the positive expression of XOD,and GD concentration were observed in the rats of each group after the treatment. Results: Compared to those in the model group,the activities of XOD and ADA were significantly decreased in the Shenshu group and in the Ganshu group( P < 0. 01); the positive expression of XOD was also significantly decreased in these two groups( P < 0. 01); GD concentration was lowered in the Ganshu group( P < 0. 01). Compared to that in the Shenshu group,the positive expression of XOD was significantly lower in the Ganshu group( P < 0. 05). Conclusion: Acupuncture intervention can reduce SUA level in HUA rats,and its mechanism is related to the metabolic enzymes XOD,ADA and GD,which may inhibit the metabolic pathway of purine decomposition and reduce the generation of uric acid.
引文
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[18]张一兵,张淑芹,周文兴,等.鸟嘌呤脱氨酶速率法测定及其对肝病的诊断价值[J].检验医学,2010,25(8):625-627.
[2]陈涛,李卫,王杨,等.高尿酸血症的患病情况及相关因素分析[J].中华临床医师杂志(电子版),2012,6(13):3526-3529.
[3]李静.高尿酸血症的流行病学研究[J].中国心血管杂志,2016,21(2):83-86.
[4]何清.高尿酸血症和痛风的病因与流行病学[J].中国临床医生,2009,37(1):11-13.
[5]朱君,余俊文.高尿酸血症和痛风的流行病学及其危险因素的研究进展[J].现代生物医学进展,2008,8(1):191-195.
[6]郑敏,麻骏武.高尿酸血症和痛风的遗传学研究进展[J].遗传,2016,38(4):300-313.
[7] Rodriguez-Hernandez JL,Rodriguez-Gonzalez F,Riano-Ruiz M,et al. Risk factors for hyperuricemia in congenital heart disease patients and its relation to cardiovascular death[J]. Congenital Heart Disease,2018,13(5):655-662.
[8]王心怡.针刺俞、原、募穴对高尿酸血症大鼠SUA、XOD、ALP的影响[D].北京:北京中医药大学,2017.
[9]卢翠娜,谢丽琴,李丽霞,等.火针对急性痛风性关节炎大鼠NALP3、IL-1β表达的影响[J].上海中医药杂志,2018,52(3):81-86.
[10] Sunagawa S,Shirakura T,Hokama N,et al. Activity of xanthine oxidase in plasma correlates with indices of insulin resistance and liver dysfunction in Japanese patients with type 2 diabetes mellitus and metabolic syndrome:A pilot exploratory study[J]. Journal of Diabetes Investigation,2019,10(1):94-103.
[11]于曙光,徐斌.实验针灸学[M].北京:人民卫生出版社,2013:269-272.
[12]林志健,刘小青,高新颜,等.高嘌呤食饵诱导的鹌鹑尿酸及糖脂代谢紊乱模型的发病特点与病理机制研究[J].中国病理生理杂志,2010,26(12):2458-2460.
[13]张红石,王富春.合募配穴与俞原配穴在脏腑辨证中的应用[J].中国针灸,2006,26(5):378-380.
[14]黄胜男,林志健,张冰,等.菊苣干预高尿酸血症鹌鹑尿酸及相关代谢酶活性研究[J].中药新药与临床药理,2015,26(1):9-13.
[15]王晓云,王洪玲,张亚梅,等.西伯利亚蓼醇提物对高尿酸血症小鼠尿酸生成和排泄的影响研究[J].中药新药与临床药理,2015,26(5):626-631.
[16] Naomi S. New agents for the treatment of gout andhyperuricemia:Febuxostat,Puricase and Beyond[J]. Current Rheumatology Reports,2010,12(2):34.
[17] Robinson PC,Dalbeth N. Febuxostat for the treatment of hyperuricaemia in gout[J]. Expert Opinion on Pharmacotherapy,2018,19(11):1289-1299.
[18]张一兵,张淑芹,周文兴,等.鸟嘌呤脱氨酶速率法测定及其对肝病的诊断价值[J].检验医学,2010,25(8):625-627.