绵羊FGF5基因Exon 3多态性对毛用性状的影响
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摘要
为探寻不同细毛羊品种FGF5基因多态性及其对毛用性状的影响,采用PCR-SSCP方法对苏博美利奴羊和东北细毛羊两个群体的FGF5基因Exon 3区进行了多态性分析,并在苏博美利奴羊群体中对不同基因型个体与其毛用性状进行了差异显著性分析。结果表明:苏博美利奴羊群体FGF5基因Exon 3区存在5种基因型,分别是DD、EE、FF、DE和EF,而东北细毛羊群体只存在DD、EE和DE三种基因型;苏博美利奴羊F等位基因由CDs区c.618和c.733多态位点构成,其中c.733位点C->G突变导致E等位基因型个体氨基酸L->V变异。苏博美利奴羊群体只有FF基因型个体在产毛量指标上显著高于DD基因型个体(P<0.05)。不同毛用绵羊品种FGF5基因多态性及其与毛用性状相关性的研究可为利用FGF5基因进行毛用性状选育奠定基础。
In order toresearch the relationship between polymorphisms on FGF5 gene and wool traits in different sheep populations,the polymorphisms at the exon 3 region of FGF5 gene in Super merino sheep and Dongbei fine wool sheep were detected by PCR-SSCP,and significant difference analysis was ran to test the effect of genotype on wool traits in Super merino sheep. The PCR-SSCP results indicated that there were 5 types of genotype in Super merino sheep,they were DD,EE,FF,DE and EF respectively. However,there were only 3 types of genotype in Dongbei fine wool sheep,they were DD,EE and DE respectively. It was possible that Super merino sheep got F allele from its male parent(Australia merino sheep)in the cross-breeding. The DNA sequencing found that there were two SNPs on c.618 and c.733 sites which constructed different genotypes. The mutant of C->G on c.733 caused the L->V mutant at protein in E allele. The significant difference analysis showed that the wool yield of FF genotype individuals was higher than that of DD individuals(P<0.05). In conclusion,the discoveries on relationship between the polymorphisms of FGF5 gene and wool traits would be useful in fine wool sheep breedingby FGF5 gene.
In order toresearch the relationship between polymorphisms on FGF5 gene and wool traits in different sheep populations,the polymorphisms at the exon 3 region of FGF5 gene in Super merino sheep and Dongbei fine wool sheep were detected by PCR-SSCP,and significant difference analysis was ran to test the effect of genotype on wool traits in Super merino sheep. The PCR-SSCP results indicated that there were 5 types of genotype in Super merino sheep,they were DD,EE,FF,DE and EF respectively. However,there were only 3 types of genotype in Dongbei fine wool sheep,they were DD,EE and DE respectively. It was possible that Super merino sheep got F allele from its male parent(Australia merino sheep)in the cross-breeding. The DNA sequencing found that there were two SNPs on c.618 and c.733 sites which constructed different genotypes. The mutant of C->G on c.733 caused the L->V mutant at protein in E allele. The significant difference analysis showed that the wool yield of FF genotype individuals was higher than that of DD individuals(P<0.05). In conclusion,the discoveries on relationship between the polymorphisms of FGF5 gene and wool traits would be useful in fine wool sheep breedingby FGF5 gene.
引文
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[16] Li W R,Liu C X,Zhang X M,et al. CRISPR/Cas9-mediated loss of FGF5 function increases wool staple length in sheep[J]. The FEBS Journal,2017,284(17):2764-2773.
[17] Hu R,Fan Z Y,Wang B Y,et al. Rapid communication:Generation of FGF5 knockout sheep via the CRISPR/Cas9 system[J]. Journal of Animal Science,2017,95(5):2019-2024.
[18]高爱琴,李宁,赵兴波,等.不同绵羊品种FGF5基因的多态性分析[J].畜牧兽医学报,2006,37(4):326-330.
[19]高爱琴,李宁,李金泉,等.山羊FGF5基因单核苷酸多态性群体遗传学分析[J].华北农学报,2006,21(3):71-76.
[20]刘海英,杨桂芹,张微,等. FGF5基因对内蒙古绒山羊绒毛性状的影响[J].遗传,2009,31(2):175-179.
[21] Bolormaa S,Swan A A,Brown D J,et al. Multiple-trait QTL mapping and genomic prediction for wool traits in sheep[J]. Genetics,Selection,Evolution:GSE,2017,49(1):62.
[22]李春笑,蒋美山,陈仕毅,等.兔成纤维细胞生长因子5(FGF5)基因SNP及其与产毛量的相关分析[J].遗传,2008,30(7):893-899.
[2] Sundberg J P,Rourk M H,Boggess D,et al. Angora mouse mutation:altered hair cycle,follicular dystrophy,phenotypic maintenance of skin grafts,and changes in keratin expression[J]. Veterinary Pathology,1997,34(3):171-179.
[3] Mizuno S,Iijima S,Okano T,et al. Retrotransposon-mediated Fgf5(go-Utr)mutant mice with long pelage hair[J]. Experimental Animals,2011,60(2):161-167.
[4] Yoshizawa Y,Wada K,Shimoi G,et al. A 1-bp deletion in Fgf5 caues male-dominant longhair in the Syrian hamster[J]. Mammalian Genome:Official Journal of the International Mammalian Genome Society,2015,26(11-12):630-637.
[5] Dr觟gemüller C,Rüfenacht S,Wichert B,et al. Mutations within the FGF5 gene are associated with hair length in cats[J].Animal Genetics,2007,38(3):218-221.
[6] Housley D J E,Venta P J. The long and the short of it:evidence that FGF5 is a major determinant of canine‘hair’-itability[J]. Animal Genetics,2006,37(4):309-315.
[7] Dierks C,M觟mke S,Philipp U,et al. Allelic heterogeneity of FGF5mutations causes the long-hair phenotype in dogs[J].Animal Genetics,2013,44(4):425-431.
[8]宦霞娟,陈玉林.獭兔KAP和FGF5基因的多态性与经济性状的关系[J].西北农业学报,2009,18(6):12-17.
[9] Legrand R,Tiret L,Abitbol M. Two recessive mutations in FGF5 are associated with the long-hair phenotype in donkeys[J]. Genetics,Selection,Evolution:GSE,2014,46:65.
[10] Daverio M S,Vidal-Rioja L,Frank E N,et al. Molecular characterization of the llama FGF5 gene and identification of putative loss of function mutations[J]. Animal Genetics,2017,48(6):716-719.
[11] Higgins C A,Petukhova L,Harel S,et al. FGF5 is a crucial regulator of hair length in humans[J]. Proceedings of the National Academy of SciencesoftheUnitedStatesofAmerica,2014,111(29):10648-10653.
[12] He X,Chao Y,Zhou G,et al. Fibroblast growth factor 5-short(FGF5s)inhibits the activity of FGF5 in primary and secondary hair follicle dermal papilla cells of cashmere goats[J]. Gene,2016,575(2Pt 2):393-398.
[13] Zhang L,He S,Liu M,et al. Molecular cloning,characterization,and expression of sheep FGF5 gene[J]. Gene,2015,555(2):95-100.
[14]刘伍限,贾斌,石国庆,等.绵羊FGF5基因的克隆、表达及RNA干扰[J].遗传,2011,33(9):982-988.
[15] Li W R,He S G,Liu C X,et al. Ectopic expression of FGF5s induces wool growth in Chinese merino sheep[J]. Gene,2017,627:477-483.
[16] Li W R,Liu C X,Zhang X M,et al. CRISPR/Cas9-mediated loss of FGF5 function increases wool staple length in sheep[J]. The FEBS Journal,2017,284(17):2764-2773.
[17] Hu R,Fan Z Y,Wang B Y,et al. Rapid communication:Generation of FGF5 knockout sheep via the CRISPR/Cas9 system[J]. Journal of Animal Science,2017,95(5):2019-2024.
[18]高爱琴,李宁,赵兴波,等.不同绵羊品种FGF5基因的多态性分析[J].畜牧兽医学报,2006,37(4):326-330.
[19]高爱琴,李宁,李金泉,等.山羊FGF5基因单核苷酸多态性群体遗传学分析[J].华北农学报,2006,21(3):71-76.
[20]刘海英,杨桂芹,张微,等. FGF5基因对内蒙古绒山羊绒毛性状的影响[J].遗传,2009,31(2):175-179.
[21] Bolormaa S,Swan A A,Brown D J,et al. Multiple-trait QTL mapping and genomic prediction for wool traits in sheep[J]. Genetics,Selection,Evolution:GSE,2017,49(1):62.
[22]李春笑,蒋美山,陈仕毅,等.兔成纤维细胞生长因子5(FGF5)基因SNP及其与产毛量的相关分析[J].遗传,2008,30(7):893-899.