日粮纤维水平对鹅盲肠细菌多样性的影响
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摘要
旨在探讨不同纤维水平日粮对卡洛斯鹅盲肠细菌多样性的影响。选用35日龄健康卡洛斯公鹅90只,随机分成5%纤维水平组(L组)和8%纤维水平组(H组),每组3个重复,每个重复15只鹅,试验期为42d。试验结束后,采用高通量测序方法对鹅盲肠黏膜细菌进行分析。结果表明,H组检测出的细菌属于17门类的199个属,L组检测出的细菌属于19门类的226个属;H组和L组细菌多样性无显著差异(P>0.05);H组Verrucomicrobia、Deferribacteres菌门和Desulfovibrio、Prevotella、Helicobacter、Mucispirillum、Akkermansia菌属相对丰度显著高于L组(P<0.05),L组Synergistetes、Euryarchaeota菌门和Bacteroides、Methanobrevibacter菌属相对丰度显著高于H组(P<0.05)。结果提示,5%和8%日粮纤维水平对鹅盲肠细菌多样性无显著影响,但可显著影响Desulfovibrio、Prevotella、Helicobacter、Mucispirillum、Akkermansia、Bacteroides、Methanobrevibacter菌属的相对丰度。
The objective of this study was to investigate the effects of different dietary fiber levels on cecum microflora diversity of Carlos geese.Ninety healthy Carlos male geese with 35 days old were divided into 2 groups(n=45)with 3 duplicates for 15 per duplicate,and then fed with diets containing 5%(L group)or 8%(H group)crude fiber using alfalfa as the crude fiber sources,respectively.After feeding 42 days,caecum microflora was determined using MiSeq PE300 sequencing platform.The results showed that caecum microflora of goose in H group included 17 phylums and 199 genera,while caecum microflora of goose in L group included 19 phylums and 226 genera.The bacteria diversity of each sample between the two groups was similar(P>0.05).The relative abundance of Verrucomicrobia,Deferribacteres,Desulfovibrio,Prevotella,Helicobacter,Mucispirillumand Akkermansia of H group significantly increased compared to L group(P<0.05);The relative abundance of Synergistetes,Euryarchaeota,Bacteroides and Methanobrevibacter of L group significantly increased compared to H group(P<0.05).These results suggest that there is no significant difference in the bacteria diversity of cecum of goose fed with5% or 8% crude fiber,however,the relative abundance of Desulfovibrio,Prevotella,Helicobacter,Mucispirillum,Akkermansia,Bacteroides,Methanobrevibacter are significantly different between the two groups.
The objective of this study was to investigate the effects of different dietary fiber levels on cecum microflora diversity of Carlos geese.Ninety healthy Carlos male geese with 35 days old were divided into 2 groups(n=45)with 3 duplicates for 15 per duplicate,and then fed with diets containing 5%(L group)or 8%(H group)crude fiber using alfalfa as the crude fiber sources,respectively.After feeding 42 days,caecum microflora was determined using MiSeq PE300 sequencing platform.The results showed that caecum microflora of goose in H group included 17 phylums and 199 genera,while caecum microflora of goose in L group included 19 phylums and 226 genera.The bacteria diversity of each sample between the two groups was similar(P>0.05).The relative abundance of Verrucomicrobia,Deferribacteres,Desulfovibrio,Prevotella,Helicobacter,Mucispirillumand Akkermansia of H group significantly increased compared to L group(P<0.05);The relative abundance of Synergistetes,Euryarchaeota,Bacteroides and Methanobrevibacter of L group significantly increased compared to H group(P<0.05).These results suggest that there is no significant difference in the bacteria diversity of cecum of goose fed with5% or 8% crude fiber,however,the relative abundance of Desulfovibrio,Prevotella,Helicobacter,Mucispirillum,Akkermansia,Bacteroides,Methanobrevibacter are significantly different between the two groups.
引文
[1]陈晓霞,杨连玉.日粮纤维构成对鹅营养物质利用的影响[J].黑龙江畜牧兽医,2017(18):59-60.CHEN X X,YANG L Y.Effects of dietary fiber composition on the utilization of goose nutrition[J].Heilongjiang Animal Science and Veterinary Medicine,2017(18):59-60.(in Chinese)
[2]杨艳红,龙向华,吕景智,等.饲粮纤维对单胃动物肠道的影响[J].中国畜牧杂志,2015,51(5):81-85.YANG Y H,LONG X H,LüJ Z,et al.Effect of dietary fiber on intestines of monogastric animals[J].Chinese Journal of Animal Science,2015,51(5):81-85.(in Chinese)
[3]刘蓓一.扬州鹅肠道微生物多样性及其受饲粮纤维水平的调控研究[D].扬州:扬州大学,2012.LIU B Y.Molecular diversity of microoanisms in the gut of Yangzhou geese and its consequences of dietary fiber level variety[D].Yangzhou:Yangzhou University,2012.(in Chinese)
[4]黄萌,刘国君,郭镇华,等.鹅消化道内微生态的研究进展[J].黑龙江畜牧兽医,2016(7):78-80.HUANG M,LIU G J,GUO Z H,et al.Research progress of goose gastrointestinal microbiota[J].Heilongjiang Animal Science and Veterinary Medicine,2016(7):78-80.(in Chinese)
[5]KATO S,HARUTA S,CUI Z J,et al.Stable coexistence of five bacterial strains as a cellulose-degrading community[J].Appl Environ Microbiol,2005,71(11):7099-7016.
[6]胡平,施寿荣,王志跃,等.采用变性梯度凝胶电泳技术研究不同形态玉米日粮对鹅肠道微生物区系的影响[J].动物营养学报,2010,22(1):169-175.HU P,SHI S R,WANG Z Y,et al.Effects of different corn diet forms on intestinal microflora of geese by denaturing gradient gel electrophoresis[J].Chinese Journal of Animal Nutrition,2010,22(1):169-175.(in Chinese)
[7]马凤莲.饲喂不同纤维分解菌对鹅肠道菌群多样性的影响[D].长春:吉林农业大学,2012.MA F L.Feeding different cellulolytic bacteria impact on intestinal microflora of geese[D].Changchun:Jilin Agricultural University,2012.(in Chinese)
[8]赫忠睿.日粮纤维对吉林白鹅消化功能及微生物多样性的影响[D].长春:吉林农业大学,2015.HE Z R.Effects of dietary fiber on digestive function and microbial diversity of Jilin goose[D].Changchun:Jilin Agricultural University,2015.(in Chinese)
[9]战利.高水平日粮纤维对鹅肠道菌群多样性的影响及部分差异菌株的确定[D].长春:吉林农业大学,2012.ZHAN L.Influence of high levels of dietary fiber on the goose road flora diversity and some difference strains were ditermined[D].Changchun:Jilin Agricultural University,2012.(in Chinese)
[10]SHIN N R,LEE J C,LEE H Y,et al.An increase in the Akkermansiaspp.population induced by metformin treatment improves glucose homeostasis in diet-induced obese mice[J].Gut,2014,63(5):727-735.
[11]MUYZER G,SMALLA K.Application of denaturing gradient gel electrophoresis(DGGE)and temperature gradient gel electrophoresis(TGGE)in microbial ecology[J].Antonie Van Leeuwenhoek,1998,73(1):127-141.
[12]LUO C W,TSEMENTZI D,KYRPIDES N,et al.Direct comparisons of Illumina vs.Roche 454sequencing technologies on the same microbial community DNA sample[J].PLoS One,2012,7(2):e30087.
[13]DEGNAN P H,OCHMAN H.Illumina-based analysis of microbial community diversity[J].ISME J,2012,6(1):183-194.
[14]PO KA J,REBECCHI A,PISACANE V,et al.Bacterial diversity in typical Italian salami at different ripening stages as revealed by high-throughput sequencing of 16SrRNA amplicons[J].Food Microbiol,2015,46:342-356.
[15]汪文强,赵生国,马利青,等.动物基因组学重测序的应用研究进展[J].畜牧兽医学报,2016,47(10):1947-1953.WANG W Q,ZHAO S G,MA L Q,et al.The research progress and application of resequencing based on animal genomics[J].Acta Veterinaria et Zootechnica Sinica,2016,47(10):1947-1953.(in Chinese)
[16]MAGOC T,SALZBERG S L.FLASH:fast length adjustment of short reads to improve genome assemblies[J].Bioinformatics,2011,27(21):2957-2963.
[17]CAPORASO J G,KUCZYNSKI J,STOMBAUGH J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nat Methods,2010,7(5):335-336.
[18]李红梅,白林,姜冬梅,等.基于16SrDNA高通量测序方法检测猪舍空气微生物多样性[J].中国畜牧杂志,2015,51(3):81-84.LI H M,BAI L,JIANG D M,et al.Microbial diversity of piggery air detected by 16S rDNA highthroughput sequencing[J].Chinese Journal of Animal Science,2015,51(3):81-84.(in Chinese)
[19]EDGAR R C.UPARSE:highly accurate OTU sequences from microbial amplicon reads[J].Nat Methods,2013,10(10):996-998.
[20]王晔,于辉.日粮粗纤维水平对鹅肠道发育和免疫器官指数的影响[J].黑龙江畜牧兽医,2015(18):83-84.WANG Y,YU H.Effects of dietary crude fiber level on intestinal development and immune organ index in goose[J].Heilongjiang Animal Science and Veterinary Medicine,2015(18):83-84.(in Chinese)
[21]周映华,胡新旭,卞巧,等.无抗发酵饲料对生长育肥猪生长性能、肠道菌群和养分表观消化率的影响[J].动物营养学报,2015,27(3):870-877.ZHOU Y H,HU X X,BIAN Q,et al.Effects of fermented feed without antibiotic on growth performance,intestinal flora and nutrient apparent digestibility of growing-finishing pigs[J].Chinese Journal of Animal Nutrition,2015,27(3):870-877.(in Chinese)
[22]孙翠丽,张阁,程汝佳,等.16SrRNA高通量测序方法检测羊圈空气微生物群落结构及多样性[J].畜牧兽医学报,2017,48(7):1314-1322.SUN C L,ZHANG G,CHENG R J,et al.Microbial community structure and diversity of sheepfold atmosphere by 16SrRNA high-throughput sequencing[J].Acta Veterinaria et Zootechnica Sinica,2017,48(7):1314-1322.(in Chinese)
[23]张名爱,王宝维,龙芳羽,等.日粮纤维水平对鹅肠道正常菌群的影响[J].福建农林大学学报:自然科学版,2007,36(2):159-162.ZHANG M A,WANG B W,LONG F Y,et al.Effect of different fibre level diets on normal microbiological floras in goose intestines[J].Journal of Fujian Agriculture and Forestry University:Natural Science Edition,2007,36(2):159-162.(in Chinese)
[24]GUO P,MOCHIDZUKI K,CHENG W,et al.Effects of different pretreatment strategies on corn stalk acidogenic fermentation using a microbial consortium[J].Bioresour Technol,2011,102(16):7526-7531.
[25]AWATI A,KONSTANTINOV S R,WILLIAMS B A,et al.Effect of substrate adaptation on the microbial fermentation and microbial composition of faecal microbiota of weaning piglets studied in vitro[J].J Sci Food Agr,2005,85(10):1765-1772.
[26]王佳婧.厌氧复合菌系的筛选及含纤维素原料预处理复合菌剂的研究[D].成都:中国科学院成都生物研究所,2009.WANG J J.Studies on screening anerobic microbial consortia and pretreatment of cellulose raw material by microbial agent[D].Chengdu:Chengdu Institute of Biology,Chinese Academy of Sciences,2009.(in Chinese)
[27]李志鹏.梅花鹿瘤胃微生物多样性与优势菌群分析[D].北京:中国农业科学院,2013.LI Z P.The analysis of microorganism diversity and dominant bacterial communities in the rumen of sika deer(Cervus nippon)[D].Beijing:Chinese Academy of Agricultural Sciences,2013.(in Chinese)
[2]杨艳红,龙向华,吕景智,等.饲粮纤维对单胃动物肠道的影响[J].中国畜牧杂志,2015,51(5):81-85.YANG Y H,LONG X H,LüJ Z,et al.Effect of dietary fiber on intestines of monogastric animals[J].Chinese Journal of Animal Science,2015,51(5):81-85.(in Chinese)
[3]刘蓓一.扬州鹅肠道微生物多样性及其受饲粮纤维水平的调控研究[D].扬州:扬州大学,2012.LIU B Y.Molecular diversity of microoanisms in the gut of Yangzhou geese and its consequences of dietary fiber level variety[D].Yangzhou:Yangzhou University,2012.(in Chinese)
[4]黄萌,刘国君,郭镇华,等.鹅消化道内微生态的研究进展[J].黑龙江畜牧兽医,2016(7):78-80.HUANG M,LIU G J,GUO Z H,et al.Research progress of goose gastrointestinal microbiota[J].Heilongjiang Animal Science and Veterinary Medicine,2016(7):78-80.(in Chinese)
[5]KATO S,HARUTA S,CUI Z J,et al.Stable coexistence of five bacterial strains as a cellulose-degrading community[J].Appl Environ Microbiol,2005,71(11):7099-7016.
[6]胡平,施寿荣,王志跃,等.采用变性梯度凝胶电泳技术研究不同形态玉米日粮对鹅肠道微生物区系的影响[J].动物营养学报,2010,22(1):169-175.HU P,SHI S R,WANG Z Y,et al.Effects of different corn diet forms on intestinal microflora of geese by denaturing gradient gel electrophoresis[J].Chinese Journal of Animal Nutrition,2010,22(1):169-175.(in Chinese)
[7]马凤莲.饲喂不同纤维分解菌对鹅肠道菌群多样性的影响[D].长春:吉林农业大学,2012.MA F L.Feeding different cellulolytic bacteria impact on intestinal microflora of geese[D].Changchun:Jilin Agricultural University,2012.(in Chinese)
[8]赫忠睿.日粮纤维对吉林白鹅消化功能及微生物多样性的影响[D].长春:吉林农业大学,2015.HE Z R.Effects of dietary fiber on digestive function and microbial diversity of Jilin goose[D].Changchun:Jilin Agricultural University,2015.(in Chinese)
[9]战利.高水平日粮纤维对鹅肠道菌群多样性的影响及部分差异菌株的确定[D].长春:吉林农业大学,2012.ZHAN L.Influence of high levels of dietary fiber on the goose road flora diversity and some difference strains were ditermined[D].Changchun:Jilin Agricultural University,2012.(in Chinese)
[10]SHIN N R,LEE J C,LEE H Y,et al.An increase in the Akkermansiaspp.population induced by metformin treatment improves glucose homeostasis in diet-induced obese mice[J].Gut,2014,63(5):727-735.
[11]MUYZER G,SMALLA K.Application of denaturing gradient gel electrophoresis(DGGE)and temperature gradient gel electrophoresis(TGGE)in microbial ecology[J].Antonie Van Leeuwenhoek,1998,73(1):127-141.
[12]LUO C W,TSEMENTZI D,KYRPIDES N,et al.Direct comparisons of Illumina vs.Roche 454sequencing technologies on the same microbial community DNA sample[J].PLoS One,2012,7(2):e30087.
[13]DEGNAN P H,OCHMAN H.Illumina-based analysis of microbial community diversity[J].ISME J,2012,6(1):183-194.
[14]PO KA J,REBECCHI A,PISACANE V,et al.Bacterial diversity in typical Italian salami at different ripening stages as revealed by high-throughput sequencing of 16SrRNA amplicons[J].Food Microbiol,2015,46:342-356.
[15]汪文强,赵生国,马利青,等.动物基因组学重测序的应用研究进展[J].畜牧兽医学报,2016,47(10):1947-1953.WANG W Q,ZHAO S G,MA L Q,et al.The research progress and application of resequencing based on animal genomics[J].Acta Veterinaria et Zootechnica Sinica,2016,47(10):1947-1953.(in Chinese)
[16]MAGOC T,SALZBERG S L.FLASH:fast length adjustment of short reads to improve genome assemblies[J].Bioinformatics,2011,27(21):2957-2963.
[17]CAPORASO J G,KUCZYNSKI J,STOMBAUGH J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nat Methods,2010,7(5):335-336.
[18]李红梅,白林,姜冬梅,等.基于16SrDNA高通量测序方法检测猪舍空气微生物多样性[J].中国畜牧杂志,2015,51(3):81-84.LI H M,BAI L,JIANG D M,et al.Microbial diversity of piggery air detected by 16S rDNA highthroughput sequencing[J].Chinese Journal of Animal Science,2015,51(3):81-84.(in Chinese)
[19]EDGAR R C.UPARSE:highly accurate OTU sequences from microbial amplicon reads[J].Nat Methods,2013,10(10):996-998.
[20]王晔,于辉.日粮粗纤维水平对鹅肠道发育和免疫器官指数的影响[J].黑龙江畜牧兽医,2015(18):83-84.WANG Y,YU H.Effects of dietary crude fiber level on intestinal development and immune organ index in goose[J].Heilongjiang Animal Science and Veterinary Medicine,2015(18):83-84.(in Chinese)
[21]周映华,胡新旭,卞巧,等.无抗发酵饲料对生长育肥猪生长性能、肠道菌群和养分表观消化率的影响[J].动物营养学报,2015,27(3):870-877.ZHOU Y H,HU X X,BIAN Q,et al.Effects of fermented feed without antibiotic on growth performance,intestinal flora and nutrient apparent digestibility of growing-finishing pigs[J].Chinese Journal of Animal Nutrition,2015,27(3):870-877.(in Chinese)
[22]孙翠丽,张阁,程汝佳,等.16SrRNA高通量测序方法检测羊圈空气微生物群落结构及多样性[J].畜牧兽医学报,2017,48(7):1314-1322.SUN C L,ZHANG G,CHENG R J,et al.Microbial community structure and diversity of sheepfold atmosphere by 16SrRNA high-throughput sequencing[J].Acta Veterinaria et Zootechnica Sinica,2017,48(7):1314-1322.(in Chinese)
[23]张名爱,王宝维,龙芳羽,等.日粮纤维水平对鹅肠道正常菌群的影响[J].福建农林大学学报:自然科学版,2007,36(2):159-162.ZHANG M A,WANG B W,LONG F Y,et al.Effect of different fibre level diets on normal microbiological floras in goose intestines[J].Journal of Fujian Agriculture and Forestry University:Natural Science Edition,2007,36(2):159-162.(in Chinese)
[24]GUO P,MOCHIDZUKI K,CHENG W,et al.Effects of different pretreatment strategies on corn stalk acidogenic fermentation using a microbial consortium[J].Bioresour Technol,2011,102(16):7526-7531.
[25]AWATI A,KONSTANTINOV S R,WILLIAMS B A,et al.Effect of substrate adaptation on the microbial fermentation and microbial composition of faecal microbiota of weaning piglets studied in vitro[J].J Sci Food Agr,2005,85(10):1765-1772.
[26]王佳婧.厌氧复合菌系的筛选及含纤维素原料预处理复合菌剂的研究[D].成都:中国科学院成都生物研究所,2009.WANG J J.Studies on screening anerobic microbial consortia and pretreatment of cellulose raw material by microbial agent[D].Chengdu:Chengdu Institute of Biology,Chinese Academy of Sciences,2009.(in Chinese)
[27]李志鹏.梅花鹿瘤胃微生物多样性与优势菌群分析[D].北京:中国农业科学院,2013.LI Z P.The analysis of microorganism diversity and dominant bacterial communities in the rumen of sika deer(Cervus nippon)[D].Beijing:Chinese Academy of Agricultural Sciences,2013.(in Chinese)