吉林白鹅毛囊发育及IGF-1、EGF基因对其发育调控的影响
|
摘要
旨在研究吉林白鹅毛囊发育以及IGF-1、EGF基因对毛囊发育过程调控的影响。选择保存7d内的吉林白鹅种蛋300枚,饲养到出雏后28d。分别在孵化期23(E23),27(E27)d,出雏后0,1,4,7,14,21,28d取样,共9个处理组,将E23作为对照组,每个处理6个重复,采用单因素完全随机试验设计,测定初级毛囊和次级毛囊密度、毛球直径、IGF-1、EGF基因在皮肤中的相对表达量。结果表明,吉林白鹅E23~28d,初级毛囊密度显著降低(P<0.05),初级毛囊毛球直径显著增加(P<0.05),次级毛囊密度显著增加(P<0.05),次级毛球直径显著增加(P<0.05)。吉林白鹅胸部为主要产绒部位。吉林白鹅E23~28d皮肤中IGF-1、EGF基因相对表达量显著增加(P<0.05)。IGF-1和EGF基因可以促进次级毛囊的发育,对吉林白鹅毛囊发育起到了调控的作用。
To study the development of hair follicles and the effects of IGF-1 and EGF genes on the regulation of hair follicles development.Three hundreds eggs saved within 7 dof Jilin white goose were choose,and raised 28 dafter brooding.Sampling were collected at hatching period 23(E23),27(E27)d,0,1,4,7,14,21,28 dafter brooding,and divided into 9 treatment groups,E23 was treated as control,each processing 6 repeated.The relative expression of primary hair follicle and secondary hair follicle density,diameter of hair follicle,IGF-1 and EGF gene in skin were determined by single factor completely randomized test.The results showed that the density of primary follicle of Jilin white goose E23-28 ddecreased significantly(P<0.05),diameter of primary follicle balls decreased significant increase(P<0.05),secondary follicle density increased significant(P<0.05),diameter of secondary wool balls increased significant(P <0.05).Jilin white goose chest is the main production of velvet.The relative expression of IGF-1 and EGF genes in the skin of Jilin white goose E23-28 dincreased significantly(P<0.05).IGF-1 and EGF genes can promote the development of secondary hair follicles and play a regulatory role in the development of hair follicles in Jilin white goose.
To study the development of hair follicles and the effects of IGF-1 and EGF genes on the regulation of hair follicles development.Three hundreds eggs saved within 7 dof Jilin white goose were choose,and raised 28 dafter brooding.Sampling were collected at hatching period 23(E23),27(E27)d,0,1,4,7,14,21,28 dafter brooding,and divided into 9 treatment groups,E23 was treated as control,each processing 6 repeated.The relative expression of primary hair follicle and secondary hair follicle density,diameter of hair follicle,IGF-1 and EGF gene in skin were determined by single factor completely randomized test.The results showed that the density of primary follicle of Jilin white goose E23-28 ddecreased significantly(P<0.05),diameter of primary follicle balls decreased significant increase(P<0.05),secondary follicle density increased significant(P<0.05),diameter of secondary wool balls increased significant(P <0.05).Jilin white goose chest is the main production of velvet.The relative expression of IGF-1 and EGF genes in the skin of Jilin white goose E23-28 dincreased significantly(P<0.05).IGF-1 and EGF genes can promote the development of secondary hair follicles and play a regulatory role in the development of hair follicles in Jilin white goose.
引文
[1]宋保国,王华雄.我国出口羽绒制品的主要质量问题分析及对策研究[J].畜牧与兽医,2010,42(2):85-87.
[2]STENN K S,COMBATES N J,EILERTSEN K J,et al.Hair follicle growth controls[J].Dermatologic Clin,1996,14(4):543-558.
[3]乔雪,王迪,司鸿飞,等.玉米地养鹅模式对北方白鹅免疫蛋白及血清生化指标的影响[J].中国兽医学报,2016,36(3):539-543.
[4]PHILPOTT M P,SANDERS D,KEALEY T.Cultured human hair follicles and growth factors[J].J Invest Dermatol,1995,104(5):44-45.
[5]HARRIS P M,MCBRIDE B W,GURNSEY M P,et al.Direct infusion of a variant of insulin-like growth factor-1into the skin of sheep and effects on local blood flow,amino acid utilization and cell replication[J].J Endocrinol,1993,139(3):463-472.
[6]姚玉园,刘海英,袁缨,等.IGF-1和EGF对辽宁绒山羊初级毛囊体外培养的影响[J].沈阳农业大学学报,2010,41(2):165-169.
[7]HUGGER J,DHEDA B S.Realtime RT-PCR normalisation strategies and considerations[J].J Genes Immun,2005,6(4):279-284.
[8]刘桂琼,曾凡同,邱祥聘.四川白鹅羽绒生长及其影响因素的研究[J].四川农业大学学报,1998,16(4):456-458.
[9]王珏,吴正祖.皖西白鹅羽区皮肤组织结构的研究及其绒被分析[J].畜牧与兽医,1995(4):159-160.
[10]李常红.吉林白鹅绒用品系皮肤及毛囊生长发育规律研究[D].吉林长春:吉林农业大学,2006.
[11]王丽.内蒙古白绒山羊皮肤毛囊发生发育规律的研究[J].畜收兽医学报,1996,27(6):524-530.
[12]刘逍,滕战伟,娄玉杰.蛋氨酸对70日龄吉林白鹅产绒性能及IGF-I基因mRNA相对表达量的影响[J].中国畜牧杂志,2017,53(1):75-78.
[13]张文慧.吉林农大白鹅羽绒品质及特性的研究[D].吉林长春:吉林农业大学,2003.
[14]牟玉婷,吴伟,孙永峰,等.BMP2基因在鹅胸、腹、背部皮肤中的定位定量表达研究[J].吉林农业大学学报,2013,35(2):241-249.
[15]赵洋,王志跃,杨海明,等.喷淋对仔鹅产绒性能和绒羽理化性质的影响[J].畜牧兽医学报,2014,45(5):781-788.
[16]崔景香,尹逊河,王慧.动物毛囊器官的分化与体外培养研究进展[J].山东农业大学学报(自然科学版),2008,39(3):489-491.
[17]李岩,廖和荣,李知勉,等.IGF-1对不同绵羊皮肤中5个毛囊相关生长因子基因表达的影响[J].中国农业科学,2007,40(3):594-600.
[18]赵改名,田玮,苏兰丽.羊毛生长的内分泌调节研究进展[J].中国畜牧杂志,2003,39(3):44.
[19]姚玉园,刘海英,袁缨,等.IGF-1和EGF对辽宁绒山羊初级毛囊体外培养的影响[J].沈阳农业大学学报,2010,41(2):165-169.
[20]HARRIS P M,MCBRIDE B W,GURNSEY M P,et al.Direct infusion of a variant of insulin-like growth factor-1into the skin of sheep and effects on local blood flow,amino acid utilization and cell replication[J].J Endocrinol,1993,139(3):463-472.
[21]王恭远,刘宁,程茄琪,等.1GF-1及其受体对小鼠皮肤作用的研究[J].中国畜牧业,2015(2):59-60.
[22]叶榕.表皮生长因子受体及其临床应用[J].医学新知杂志,2008,18(3):164-166.
[23]卿素珠,林吉茂,张灵枝,等.表皮生长因子及其受体在山羊胎儿皮肤发育中的表达特征[J].畜牧兽医学报,2009,40(9):1383-1388.
[24]杨希川,郝飞.毛囊形态发生的基因调控研究进展[J].临床与病理杂志,2002,22(6):633-636.
[25]史明艳,林峰,杨学义,等.不同浓度的EGF对山羊毛囊干细胞体外培养的影响[J].西北农业学报,2009,18(3):26-28.
[26]崔志峰,赵晶,王慧,等.不同培养基对山羊毛囊体外培养的影响研究[J].山东大学学报(理学版),2008,43(5):1-5.
[27]王睿智.济宁青山羊毛囊发育特性与EGF、IGF2基因差异表达的研究[D].山东泰安:山东农业大学,2011.
[2]STENN K S,COMBATES N J,EILERTSEN K J,et al.Hair follicle growth controls[J].Dermatologic Clin,1996,14(4):543-558.
[3]乔雪,王迪,司鸿飞,等.玉米地养鹅模式对北方白鹅免疫蛋白及血清生化指标的影响[J].中国兽医学报,2016,36(3):539-543.
[4]PHILPOTT M P,SANDERS D,KEALEY T.Cultured human hair follicles and growth factors[J].J Invest Dermatol,1995,104(5):44-45.
[5]HARRIS P M,MCBRIDE B W,GURNSEY M P,et al.Direct infusion of a variant of insulin-like growth factor-1into the skin of sheep and effects on local blood flow,amino acid utilization and cell replication[J].J Endocrinol,1993,139(3):463-472.
[6]姚玉园,刘海英,袁缨,等.IGF-1和EGF对辽宁绒山羊初级毛囊体外培养的影响[J].沈阳农业大学学报,2010,41(2):165-169.
[7]HUGGER J,DHEDA B S.Realtime RT-PCR normalisation strategies and considerations[J].J Genes Immun,2005,6(4):279-284.
[8]刘桂琼,曾凡同,邱祥聘.四川白鹅羽绒生长及其影响因素的研究[J].四川农业大学学报,1998,16(4):456-458.
[9]王珏,吴正祖.皖西白鹅羽区皮肤组织结构的研究及其绒被分析[J].畜牧与兽医,1995(4):159-160.
[10]李常红.吉林白鹅绒用品系皮肤及毛囊生长发育规律研究[D].吉林长春:吉林农业大学,2006.
[11]王丽.内蒙古白绒山羊皮肤毛囊发生发育规律的研究[J].畜收兽医学报,1996,27(6):524-530.
[12]刘逍,滕战伟,娄玉杰.蛋氨酸对70日龄吉林白鹅产绒性能及IGF-I基因mRNA相对表达量的影响[J].中国畜牧杂志,2017,53(1):75-78.
[13]张文慧.吉林农大白鹅羽绒品质及特性的研究[D].吉林长春:吉林农业大学,2003.
[14]牟玉婷,吴伟,孙永峰,等.BMP2基因在鹅胸、腹、背部皮肤中的定位定量表达研究[J].吉林农业大学学报,2013,35(2):241-249.
[15]赵洋,王志跃,杨海明,等.喷淋对仔鹅产绒性能和绒羽理化性质的影响[J].畜牧兽医学报,2014,45(5):781-788.
[16]崔景香,尹逊河,王慧.动物毛囊器官的分化与体外培养研究进展[J].山东农业大学学报(自然科学版),2008,39(3):489-491.
[17]李岩,廖和荣,李知勉,等.IGF-1对不同绵羊皮肤中5个毛囊相关生长因子基因表达的影响[J].中国农业科学,2007,40(3):594-600.
[18]赵改名,田玮,苏兰丽.羊毛生长的内分泌调节研究进展[J].中国畜牧杂志,2003,39(3):44.
[19]姚玉园,刘海英,袁缨,等.IGF-1和EGF对辽宁绒山羊初级毛囊体外培养的影响[J].沈阳农业大学学报,2010,41(2):165-169.
[20]HARRIS P M,MCBRIDE B W,GURNSEY M P,et al.Direct infusion of a variant of insulin-like growth factor-1into the skin of sheep and effects on local blood flow,amino acid utilization and cell replication[J].J Endocrinol,1993,139(3):463-472.
[21]王恭远,刘宁,程茄琪,等.1GF-1及其受体对小鼠皮肤作用的研究[J].中国畜牧业,2015(2):59-60.
[22]叶榕.表皮生长因子受体及其临床应用[J].医学新知杂志,2008,18(3):164-166.
[23]卿素珠,林吉茂,张灵枝,等.表皮生长因子及其受体在山羊胎儿皮肤发育中的表达特征[J].畜牧兽医学报,2009,40(9):1383-1388.
[24]杨希川,郝飞.毛囊形态发生的基因调控研究进展[J].临床与病理杂志,2002,22(6):633-636.
[25]史明艳,林峰,杨学义,等.不同浓度的EGF对山羊毛囊干细胞体外培养的影响[J].西北农业学报,2009,18(3):26-28.
[26]崔志峰,赵晶,王慧,等.不同培养基对山羊毛囊体外培养的影响研究[J].山东大学学报(理学版),2008,43(5):1-5.
[27]王睿智.济宁青山羊毛囊发育特性与EGF、IGF2基因差异表达的研究[D].山东泰安:山东农业大学,2011.