环境对水稻花时的影响和花时调查统计方法的改进
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摘要
花时在杂交稻制种中有着关键作用。明确环境对水稻花时的影响,选择合适的花时调查统计方法可以加快对花时性状的研究。本文以早花时突变体eft、正常花时材料日本晴(NIP)以及构建的eft/NIP重组自交系群体为材料,通过分期播种亲本,明确了温度和光照对花时的影响规律;提出了一种以亲本为标准的改进的水稻花时调查和统计方法。基于该改进的花时调查和统计方法,能有效减弱环境因素对花时性状的影响,可将样本间的花时误差由原来的0~30 min缩减至0~5 min,为水稻花时性状的遗传研究提供较为精确的数据。
Rice flowering time is a critical factor for seed production in hybrid rice. The research of rice flowering time could be pro-moted by clarification the influence of environment and utilization appropriate investigation and statistical method. In the present study, the regularity of flowering time changing with temperature and day length was clarified though sowing parents by stages. Simul-taneously, an effective investigation and statistical method was firstly established to investigate rice flowering time in the field no mat-ter how the environment changes, based on a recombinant inbred line population including 184 lines from the cross between Nippon-bare and the early flowering time mutant eft. The improved method could effectively mitigate the influence of environmental on flower-ing time and reduce the error of flowering time from 0~30 min to 0~5 min. The method may provide more accurate data to study rice flowering time traits in the future.
Rice flowering time is a critical factor for seed production in hybrid rice. The research of rice flowering time could be pro-moted by clarification the influence of environment and utilization appropriate investigation and statistical method. In the present study, the regularity of flowering time changing with temperature and day length was clarified though sowing parents by stages. Simul-taneously, an effective investigation and statistical method was firstly established to investigate rice flowering time in the field no mat-ter how the environment changes, based on a recombinant inbred line population including 184 lines from the cross between Nippon-bare and the early flowering time mutant eft. The improved method could effectively mitigate the influence of environmental on flower-ing time and reduce the error of flowering time from 0~30 min to 0~5 min. The method may provide more accurate data to study rice flowering time traits in the future.
引文
[1]Zhao Lei,Lei J ianguo,Huang Yingjin,et al.Mapping quantitative trait loci for heat tolerance at anthesis in rice using chromosomal segment substitution lines[J].Breeding Sci,2016,66:358-366.
[2]Jagadish,SVK,Craufurd PQ,Wheeler TR.High temperature stress and spikelet fertility in rice(Oryza sativa L.)[J].J Exp Bot,2007,58(7)∶1 627-1 635.
[3]Ishimaru T,Hirabayashi H,Ida M,et al.A genetic resource for early-morning flowering trait of wild rice Oryza officinalis to mitigate high temperature-induced spikelet sterility at anthesis[J].Ann Bot,2010,106:515-520.
[4]Hirabayashi HK,Sasaki T,Kambe RB,et al.q EMF3,a novel QTLfor the early-morning flowering trait from wild rice,Oryza officinalis,to mitigate heat stress damage at flowering in rice,O.sativa[J].J Exp Bot,2015,66:1 227-1 236.
[5]袁隆平.杂交水稻学[M].北京:中国农业出版社,2002.
[6]肖华伟.中国杂交水稻制种技术研究与实践及其发展趋势分析[D].长沙:湖南农业大学,2009.
[7]张萌,戴冬青,李西明,等.水稻花时性状研究进展[J].核农学报,2016,30(2):0276-0274.
[8]徐云碧,申宗坦,杨再能,等.提高水稻异交率的研究-(一)栽培稻柱头外露性变异的分析[J].浙江大学学报,1986,12(4):359-368.
[9]许克农,李泽炳,李成荃.光(温)敏核不育水稻的育性和开花习性研究[J].安徽农业科学,1992,20(4):293-301.
[10]张戟,陆建国,盛金元,等.籼粳交后代柱头外露率的遗传表现与选择[J].江苏农业科学,2000(2):6-9.
[11]陈立云.两系法杂交水稻的理论与技术[M].上海:上海科学技术出版社,2001:126.
[12]陆燕雯,王冬翼,顾庆华,等.粳型光温敏感雄性核不育系开花习性研究[J].上海农业学报,2012,28(3):124-126.
[13]龙辉.滇型杂交稻滇杂33高产制种技术研究[J].现代农业科技,2013,42(1):29-30.
[14]仲维功,李传国,苏自强.不同类型水稻品种花时特性的研究[J].江苏农业学报,1990,18(1):16-18.
[15]李金军,范国华,张仁余,等.不同水稻品种开花时间的比较试验[J].浙江农业学报,1997(1):63-66.
[16]黄友明.雄性不育水稻的颖花开放习性研究综述[J].宜春学院学报,2009,31(4):110-112.
[17]蒯建敏,莫惠栋,惠大丰.水稻花时与气象因子的关系[J].中国水稻科学,1994,8(2):79-84.
[18]马启林.杂交水稻制种花时不遇的生态生理机制研究Ⅱ.温湿互作配比与花时相遇的关系[J].湖北农学院学报,1999,19(3):193-197.
[19]马作斌,詹瞻,程效义,等.花时性状在籼粳交后代中的表现及其对外部环境的响应[J].杂交水稻,2011,26(5):70-76.
[20]王开锡,林庆泉,杨聚宝.籼粳稻花时的分布调查[J].福建稻麦科技,1990(3):10-15.
[21]万国,冯跃,张凤娇,等.水稻花时性状的QTL定位[J].核农学报,2013,27(5):562-567.
[22]Kobayasi K,Matsui T,Yoshimoto M,et al.Effects of temperature,solar radiation,and vapor-pressure deficit on flower opening time in rice[J].Plant production sci,2010,13(1):21-28.
[23]马启林,胡达明,刘百韬.杂交水稻制种花时不遇的生态生理机制研究Ⅰ.花时同步指数研究[J].湖北农学院学报,1997,17(2):91-96.
[24]曾晓春,周燮.茉莉酸甲酯(MeJ A)诱导水稻颖花开放[J].植物学报,1999,41(5):560-562.
[25]林建荣,吴明国,宋昕蔚.三系粳稻不育系开花习性与异交结实率的关系[J].杂交水稻,2006,21(5):69-72.
[2]Jagadish,SVK,Craufurd PQ,Wheeler TR.High temperature stress and spikelet fertility in rice(Oryza sativa L.)[J].J Exp Bot,2007,58(7)∶1 627-1 635.
[3]Ishimaru T,Hirabayashi H,Ida M,et al.A genetic resource for early-morning flowering trait of wild rice Oryza officinalis to mitigate high temperature-induced spikelet sterility at anthesis[J].Ann Bot,2010,106:515-520.
[4]Hirabayashi HK,Sasaki T,Kambe RB,et al.q EMF3,a novel QTLfor the early-morning flowering trait from wild rice,Oryza officinalis,to mitigate heat stress damage at flowering in rice,O.sativa[J].J Exp Bot,2015,66:1 227-1 236.
[5]袁隆平.杂交水稻学[M].北京:中国农业出版社,2002.
[6]肖华伟.中国杂交水稻制种技术研究与实践及其发展趋势分析[D].长沙:湖南农业大学,2009.
[7]张萌,戴冬青,李西明,等.水稻花时性状研究进展[J].核农学报,2016,30(2):0276-0274.
[8]徐云碧,申宗坦,杨再能,等.提高水稻异交率的研究-(一)栽培稻柱头外露性变异的分析[J].浙江大学学报,1986,12(4):359-368.
[9]许克农,李泽炳,李成荃.光(温)敏核不育水稻的育性和开花习性研究[J].安徽农业科学,1992,20(4):293-301.
[10]张戟,陆建国,盛金元,等.籼粳交后代柱头外露率的遗传表现与选择[J].江苏农业科学,2000(2):6-9.
[11]陈立云.两系法杂交水稻的理论与技术[M].上海:上海科学技术出版社,2001:126.
[12]陆燕雯,王冬翼,顾庆华,等.粳型光温敏感雄性核不育系开花习性研究[J].上海农业学报,2012,28(3):124-126.
[13]龙辉.滇型杂交稻滇杂33高产制种技术研究[J].现代农业科技,2013,42(1):29-30.
[14]仲维功,李传国,苏自强.不同类型水稻品种花时特性的研究[J].江苏农业学报,1990,18(1):16-18.
[15]李金军,范国华,张仁余,等.不同水稻品种开花时间的比较试验[J].浙江农业学报,1997(1):63-66.
[16]黄友明.雄性不育水稻的颖花开放习性研究综述[J].宜春学院学报,2009,31(4):110-112.
[17]蒯建敏,莫惠栋,惠大丰.水稻花时与气象因子的关系[J].中国水稻科学,1994,8(2):79-84.
[18]马启林.杂交水稻制种花时不遇的生态生理机制研究Ⅱ.温湿互作配比与花时相遇的关系[J].湖北农学院学报,1999,19(3):193-197.
[19]马作斌,詹瞻,程效义,等.花时性状在籼粳交后代中的表现及其对外部环境的响应[J].杂交水稻,2011,26(5):70-76.
[20]王开锡,林庆泉,杨聚宝.籼粳稻花时的分布调查[J].福建稻麦科技,1990(3):10-15.
[21]万国,冯跃,张凤娇,等.水稻花时性状的QTL定位[J].核农学报,2013,27(5):562-567.
[22]Kobayasi K,Matsui T,Yoshimoto M,et al.Effects of temperature,solar radiation,and vapor-pressure deficit on flower opening time in rice[J].Plant production sci,2010,13(1):21-28.
[23]马启林,胡达明,刘百韬.杂交水稻制种花时不遇的生态生理机制研究Ⅰ.花时同步指数研究[J].湖北农学院学报,1997,17(2):91-96.
[24]曾晓春,周燮.茉莉酸甲酯(MeJ A)诱导水稻颖花开放[J].植物学报,1999,41(5):560-562.
[25]林建荣,吴明国,宋昕蔚.三系粳稻不育系开花习性与异交结实率的关系[J].杂交水稻,2006,21(5):69-72.