2种炮制方法对何首乌中6种成分变化的影响
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摘要
目的探索"九蒸九晒"、药典法2种炮制方法对何首乌中6种成分含有量变化的影响。方法何首乌甲醇提取液的分析采用Agilent C_(18)色谱柱(250 mm×4. 6 mm,5μm);流动相乙腈-0. 01%磷酸,梯度洗脱;体积流量1. 0 mL/min;检测波长295 nm;柱温30℃。结果 6种成分在各自范围内线性关系良好(R~2>0. 995 0),平均加样回收率98. 96%～101. 23%,RSD 0. 41%～1. 37%。结论何首乌在2种炮制方法下6种成分含有量存在较大差异,这些差异是否与疗效和肝毒性相关还有待进一步探讨。
AIM To explore the effects of two processing methods( "nine steamed and nine shined" method and pharmacopoeia method) on the content changes of six constituents in Polygonum multiflorum Thunb.. METHODS The analysis of methanol extract of P. multiflorum was performed on a 30 ℃ thermostatic Agilent C_(18)column( 250 mm×4. 6 mm,5 μm),with the mobile phase comprising of acetonitrile-0. 01% phosphoric acid flowing at1. 0 mL/min in a gradient elution manner,and the detection wavelength was set at 295 nm. RESULTS Six constituents showed good linear relationships within their own ranges( R~2> 0. 995 0),whose average recoveries were98. 96%-101. 23% with the RSDs of 0. 41%-1. 37%. CONCLUSION There are great differences in the contents of six constituents in P. multiflorum between two processing methods,but whether these differences are related to efficacy and hepatotoxicity remains to be further explored.
AIM To explore the effects of two processing methods( "nine steamed and nine shined" method and pharmacopoeia method) on the content changes of six constituents in Polygonum multiflorum Thunb.. METHODS The analysis of methanol extract of P. multiflorum was performed on a 30 ℃ thermostatic Agilent C_(18)column( 250 mm×4. 6 mm,5 μm),with the mobile phase comprising of acetonitrile-0. 01% phosphoric acid flowing at1. 0 mL/min in a gradient elution manner,and the detection wavelength was set at 295 nm. RESULTS Six constituents showed good linear relationships within their own ranges( R~2> 0. 995 0),whose average recoveries were98. 96%-101. 23% with the RSDs of 0. 41%-1. 37%. CONCLUSION There are great differences in the contents of six constituents in P. multiflorum between two processing methods,but whether these differences are related to efficacy and hepatotoxicity remains to be further explored.
引文
[1]国家药典委员会.中华人民共和国药典:2015年版一部[S].北京:中国医药科技出版社,2015:175-177.
[2]顾蘅,张毅.何首乌炮制方法古今研究[J].中医临床研究,2015,7(34):24-26.
[3]刘振丽,宋志前,张玲,等.不同炮制工艺对何首乌中成分含量的影响[J].中国中药杂志,2005,30(5):336-340.
[4]丘小惠,马兴田,闵江,等.何首乌炮制工艺的研究[J].中国药房,2006,17(16):1270-1273.
[5] Tang L,Sui L,Ya Y,et al. Potential antioxidant components and characteristics of fresh Polygonum multiflorum[J]. J Food Drug Anal,2010,18(2):120-127.
[6] Lv G,Lou Z,Chen S,et al. Pharmacokinetics and tissue distribution of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside from traditional Chinese medicine Polygonum multiflorum following oral administration to rats[J]. J Ethnopharmacol,2011,137(1):449-456.
[7] Lv G,Gu H,Chen S,et al. Pharmacokinetic profile of 2,3,5,4'-tetra hydroxystilbene-2-O-β-D-glucoside in mice after oral administration of Polygonum multiflorum extract[J]. Drug Dev Ind Pharm,2012,38(2):248-255.
[8]苏颂.图经本草辑复本[M].胡乃长,王致谱辑注.福州:福建科学技术出版社,1988:286.
[9]潘雪梅,房德敏.制何首乌致肝损伤毒性的研究进展[J].天津药学,2015,27(6):57-61.
[10] Ma J,Zheng L,He Y S,et al. Hepatotoxic assessment of Polygoni Multiflori Radix extract and toxicokinetic study of stilbene glucoside and anthraquinones in rats[J]. J Ethnopharmacol,2015,162:61-68.
[11]杨艳菁,兰才武,贺定翔,等.不同加工方法对何首乌饮片质量的影响[J].中国实验方剂学杂志,2015,21(15):31-34.
[2]顾蘅,张毅.何首乌炮制方法古今研究[J].中医临床研究,2015,7(34):24-26.
[3]刘振丽,宋志前,张玲,等.不同炮制工艺对何首乌中成分含量的影响[J].中国中药杂志,2005,30(5):336-340.
[4]丘小惠,马兴田,闵江,等.何首乌炮制工艺的研究[J].中国药房,2006,17(16):1270-1273.
[5] Tang L,Sui L,Ya Y,et al. Potential antioxidant components and characteristics of fresh Polygonum multiflorum[J]. J Food Drug Anal,2010,18(2):120-127.
[6] Lv G,Lou Z,Chen S,et al. Pharmacokinetics and tissue distribution of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside from traditional Chinese medicine Polygonum multiflorum following oral administration to rats[J]. J Ethnopharmacol,2011,137(1):449-456.
[7] Lv G,Gu H,Chen S,et al. Pharmacokinetic profile of 2,3,5,4'-tetra hydroxystilbene-2-O-β-D-glucoside in mice after oral administration of Polygonum multiflorum extract[J]. Drug Dev Ind Pharm,2012,38(2):248-255.
[8]苏颂.图经本草辑复本[M].胡乃长,王致谱辑注.福州:福建科学技术出版社,1988:286.
[9]潘雪梅,房德敏.制何首乌致肝损伤毒性的研究进展[J].天津药学,2015,27(6):57-61.
[10] Ma J,Zheng L,He Y S,et al. Hepatotoxic assessment of Polygoni Multiflori Radix extract and toxicokinetic study of stilbene glucoside and anthraquinones in rats[J]. J Ethnopharmacol,2015,162:61-68.
[11]杨艳菁,兰才武,贺定翔,等.不同加工方法对何首乌饮片质量的影响[J].中国实验方剂学杂志,2015,21(15):31-34.