宫颈癌非典型鳞状上皮细胞病变与人乳头瘤病毒亚型的关系
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摘要
目的探讨宫颈癌非典型鳞状上皮细胞病变(ASCUS)与人乳头瘤病毒(HPV)亚型的关系。方法选取2018年1月至2018年5月间山东省立第三医院和济南齐鲁医学检验所体检的210位女性体检者子宫颈细胞标本进行回顾性分析,采用液基细胞学技术(TCT)与反向斑点杂交技术对子宫颈细胞标本进行镜下观察和23种HPV亚型检测。结果 210个ASCUS标本中,HPV阳性标本144例,占比68.6%;阴性标本66例,占比31.4%。144例阳性标本中,单一低危型HPV感染9例,占比6.3%;单一次高危型HPV感染65例,占比45.1%;单一高危型HPV感染25例,占比17.4%;多重次高危型HPV感染21例,占比14.6%;高危合并次高危型HPV感染4例,占比2.8%;高危合并低危型HPV混合感染20例,占比13.9%。结论宫颈癌患者TCT检查结果为ASCUS时,建议做HPV亚型分型检测。
Objective To explore the relationship between the pathological changes of atypical squamous cells of undetermined significance(ASCUS) and human papilloma virus(HPV) subtype in the cervical cancer.Methods Cervical cell specimens in 210 women who had physical examination at Shandong Provincial Third Hospital and Qilu Medical Laboratory were retrospectively analyzed and liquid based cytology and reverse dot blot hybridization technology were used to microscopically observe the cervical cells were subtype and detect the 23 HPV subtypes between January 2018 and May 2018.Results Among the210 specimens of ASCUS specimens,144 were positive(68.6%) and 66 were negative(31.4%).Among the 144 positive specimens,9 cases were found to infect pure low-risk HPV(6.3%),65 cases infect secondary high risk HPV(45.1%),25 cases infect pure high-risk HPV(17.4%),21 cases infect multiple secondary high-risk HPV(14.6%),4 cases infect high-risk complicated with secondary high-risk HPV(2.8%) and 20 cases infect low risk complicated with high-risk HPV(13.9%).Conclusion If the results of thin-cytologic test(TCT) present ASCUS,HPV subtype detection was detected.
Objective To explore the relationship between the pathological changes of atypical squamous cells of undetermined significance(ASCUS) and human papilloma virus(HPV) subtype in the cervical cancer.Methods Cervical cell specimens in 210 women who had physical examination at Shandong Provincial Third Hospital and Qilu Medical Laboratory were retrospectively analyzed and liquid based cytology and reverse dot blot hybridization technology were used to microscopically observe the cervical cells were subtype and detect the 23 HPV subtypes between January 2018 and May 2018.Results Among the210 specimens of ASCUS specimens,144 were positive(68.6%) and 66 were negative(31.4%).Among the 144 positive specimens,9 cases were found to infect pure low-risk HPV(6.3%),65 cases infect secondary high risk HPV(45.1%),25 cases infect pure high-risk HPV(17.4%),21 cases infect multiple secondary high-risk HPV(14.6%),4 cases infect high-risk complicated with secondary high-risk HPV(2.8%) and 20 cases infect low risk complicated with high-risk HPV(13.9%).Conclusion If the results of thin-cytologic test(TCT) present ASCUS,HPV subtype detection was detected.
引文
[1]杨丹球.人乳头瘤病毒感染与子宫颈癌发病关系的研究[J].临床和实验医学杂志,2010,9:1653-1655.
[2]于春梅,郝凤艳,沈迎健.宫颈刮片同时联合阴道镜检查在妇女宫颈病变普查中的应用价值[J].中国妇幼保健,2011,26:3493-3494.
[3]胡岩.细胞DNA倍体定量分析可做为癌前病变和宫颈癌筛查的必要检测方法[J].医学信息(中旬刊),2011,24:3842-3843.
[4]胡凯红.TCT筛查宫颈病变680例临床分析[J].中国保健营养(上旬刊),2013,23:1149.
[5]喻垚,郭变琴,罗光丽,等.液基细胞学与高危型HPV-DNA联合检测在宫颈病变筛查中的意义[J]检验医学与临床,2015,12:1550-1554.
[6]廖光东,姚强.人乳头瘤病毒感染与生育相关问题[J].实用妇产科杂志,2018,34:84-85.
[7]曹喜琴,杜海军,周玲.人乳头瘤病毒感染的影响因素[J].国际病毒学杂志,2006,13:104-107.
[8]Kitamura T,Suzuki M,Koyama Y,et al.Long-term persistence of human papillomavirus in the skin of the glans penis of elderly men above 80 years of age[J].Int J STD AIDS,2018,29:552-556.
[9]Liu Z,Nyitray AG,Hwang LY,et al.Acquisition,persistence and clearance of human papillomavirus infection among male virgins residing in brazil,mexico,and the united states[J].J Infect Dis,2018,217:767-776.
[10]Sui S,Zhu M,Jiao Z,et al.Prognosis and related factors of HPV infections in postmenopausal Uyghur women[J].J Obstet Gynaecol,2018,38:1-5.
[11]程俊,雷张涛.HPV基因分型对ASCUS细胞临床应用的价值[J].中南医学科学杂志,2017,45:388-390.
[12]靳东芳,程金洛.膜式液基薄层细胞学检测联合高危型人乳头状瘤病毒分型检测在宫颈癌筛查中的应用[J].中国肿瘤临床与康复,2014,21:1314-1316.
[2]于春梅,郝凤艳,沈迎健.宫颈刮片同时联合阴道镜检查在妇女宫颈病变普查中的应用价值[J].中国妇幼保健,2011,26:3493-3494.
[3]胡岩.细胞DNA倍体定量分析可做为癌前病变和宫颈癌筛查的必要检测方法[J].医学信息(中旬刊),2011,24:3842-3843.
[4]胡凯红.TCT筛查宫颈病变680例临床分析[J].中国保健营养(上旬刊),2013,23:1149.
[5]喻垚,郭变琴,罗光丽,等.液基细胞学与高危型HPV-DNA联合检测在宫颈病变筛查中的意义[J]检验医学与临床,2015,12:1550-1554.
[6]廖光东,姚强.人乳头瘤病毒感染与生育相关问题[J].实用妇产科杂志,2018,34:84-85.
[7]曹喜琴,杜海军,周玲.人乳头瘤病毒感染的影响因素[J].国际病毒学杂志,2006,13:104-107.
[8]Kitamura T,Suzuki M,Koyama Y,et al.Long-term persistence of human papillomavirus in the skin of the glans penis of elderly men above 80 years of age[J].Int J STD AIDS,2018,29:552-556.
[9]Liu Z,Nyitray AG,Hwang LY,et al.Acquisition,persistence and clearance of human papillomavirus infection among male virgins residing in brazil,mexico,and the united states[J].J Infect Dis,2018,217:767-776.
[10]Sui S,Zhu M,Jiao Z,et al.Prognosis and related factors of HPV infections in postmenopausal Uyghur women[J].J Obstet Gynaecol,2018,38:1-5.
[11]程俊,雷张涛.HPV基因分型对ASCUS细胞临床应用的价值[J].中南医学科学杂志,2017,45:388-390.
[12]靳东芳,程金洛.膜式液基薄层细胞学检测联合高危型人乳头状瘤病毒分型检测在宫颈癌筛查中的应用[J].中国肿瘤临床与康复,2014,21:1314-1316.