棘球绦虫与棘球蚴病检测技术研究进展
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摘要
棘球蚴病是由棘球绦虫的幼虫寄生于人体引起的一种慢性寄生虫病,由于棘球绦虫生活史复杂、流行范围广泛以及宿主种类众多,控制传播和消除棘球蚴病仍较困难。在棘球蚴病早期,准确的诊断和检测,实时监测各类宿主的感染情况,从源头控制传染源和阻断传播途径非常关键。本文综述了近年来棘球绦虫检测技术及相关研究进展,包括形态学检测技术、影像学诊断技术、免疫学技术及分子生物学技术等,以期为棘球绦虫及棘球蚴病的检测与诊断提供参考。
Echinococcosis is a chronic parasitic disease caused by the infection of Echinococcus larvae. Due to the complex life cycle involved with a large number of hosts, it is very challenging to control the spread of infection and eliminate echinococcosis. Early and accurate detection of Echinococcus parasite, real-time monitoring of various types of host infection are very critical for blocking the epidemic and the transmission of the disease. This paper reviews the recent advances in the detection technology for Echinococcus parasite and the related research on the diagnosis of echinococcosis, including morphological identification, imaging diagnosis, immunological and molecular biology diagnostic techniques, in order to provide useful information for the advanced detection of Echinococcus infections and diagnosis of echinococcosis.
Echinococcosis is a chronic parasitic disease caused by the infection of Echinococcus larvae. Due to the complex life cycle involved with a large number of hosts, it is very challenging to control the spread of infection and eliminate echinococcosis. Early and accurate detection of Echinococcus parasite, real-time monitoring of various types of host infection are very critical for blocking the epidemic and the transmission of the disease. This paper reviews the recent advances in the detection technology for Echinococcus parasite and the related research on the diagnosis of echinococcosis, including morphological identification, imaging diagnosis, immunological and molecular biology diagnostic techniques, in order to provide useful information for the advanced detection of Echinococcus infections and diagnosis of echinococcosis.
引文
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[4]张梦媛,伍卫平,官亚宜,等.我国棘球蚴病疾病负担分析[J].中国寄生虫学与寄生虫病杂志,2018,36(1):15-19,25.
[5]曾祥嫚,王立英,伍卫平,等.我国非青藏高原流行区囊型棘球蚴病聚类分析[J].中国血吸虫病防治杂志,2014,(2):180-183.
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[8]伍卫平.我国两型包虫病的流行与分布情况[J].中国动物保健,2017,19(7):7-9.
[9] Carmena D,Cardona GA. Canine echinococcosis:global epidemiology and genotypic diversity[J]. Acta Trop,2013,128(3):441-460.
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[12]刘辉,肖宁,杨诗杰,等.青藏高原地区犬棘球绦虫感染的流行病学特征[J].中国血吸虫病防治杂志,2017,29(2):129-138.
[13]孟庆贺.我国包虫病的诊断和治疗进展[J].中国城乡企业卫生,2014,29(5):16-18.
[14]王虎,阎海玉,武海燕.青海高原不同宿主体内原头节的透射电镜观察[J].地方病通报,1994,(3):19-21.
[15]曾诚,韩秀敏,蔡慧霞,等.青海省青南高原与柴达木盆地细粒棘球蚴实验感染犬体内成虫形态学比较观察[J].中国病原生物学杂志,2006,1(4):257-259.
[16] Aoki T,Hagiwara M,Yabuki H,et al. Unique MRI findings for differentiation of an early stage of hepatic alveolar echinococcosis[J]. Case Reports,2015,2015(feb19 1):bcr2014208123-bcr2014208123.
[17] Surgery B. CT scanning in identification of sheep cystic echinococcosis[J].Biomed Res Int,2017,2017:1-6.
[18] Liu H,Zhang C,Fan X,et al. Robust phase-retrieval-based X-ray tomography for morphological assessment of early hepatic echinococcosis infection in rats[J]. PLoS One,2017,12(9):e183396.
[19]曾红春,王颖鑫,王俊华,等.大鼠感染肝泡状棘球蚴不同时期超声动态演变与病理对照研究[J].中国医学影像学杂志,2016,24(5):325-328.
[20] Osimani JJ,Migliaro EF,Abella CM. Experimental study of the effect of ultrasonics on the larva of Echinococcus granulosus(hydatid cyst)[J]. Ann Parasitol Hum Comp,1953,28(1/2):33-46.
[21] Gharbi HA,Hassine W,Brauner MW,et al. Ultrasound examination of the hydatic liver[J]. Radiol,1981,139(2):459-463.
[22] A ubry G,Tillier H,Massonnat J,et al. Vertebrocostal echinococcosis:presentation of specimen and X-ray pictures[J]. Alger Medicale,1952,56(10):712-719.
[23] Koufakis T,Margaritis A. Right upper quadrant pain:a case where diagnosis was made from the chest X-ray[J]. Pan Afr Med J,2014,17(1):60.
[24] Shah T,Geyt JL,Atkins J. Let sleeping dogs lie:a surprising chest X-ray in the paediatric emergency department[J].Case Rep,2014,2014(apr25 1). DOI:10.1136/bcr-2014-203973
[25] Akgul OC,Onat S. Computed tomography(CT)findings of pulmonary hydatid cysts in children and the factors related to cyst rupture[J]. Med Sci Monit,2017,23:3679-3686.
[26] Sreedevi C,Devi MA,Annapurna P,et al. A rare case of spleenic hydatidosis in a buffalo:patho-morphological study[J].J Parasitic Dis,2016,40(1):1-3.
[27] Tackmann K,Mattis R,Conraths FJ. Detection of Echinococcus multilocularis in foxes:evaluation of a protocol of the intestinal scraping technique[J]. J Vet Med B Infect Dis Vet Public Health,2006,53(8):395-398.
[28] Duscher G,Prosl H,Joachim A. Scraping or shaking--a comparison of methods for the quantitative determination of Echinococcus multilocularis in fox intestines[J]. Parasitol Res,2005,95(1):40-42.
[29] Umhang G,Woronoff-Rhen N,Combes B,et al. Segmental sedimentation and counting technique(SSCT):an adaptable method for qualitative diagnosis of Echinococcus multilocularis in fox intestines[J]. Exp Parasitol,2011,128(1):57-60.
[30]刘学庭,黄月菊.多房性棘球绦虫原头蚴生成、发育和繁殖的形态学观察[J].诊断病理学杂志,2002,9(4):234-235.
[31] SarīC,Ertug S,Karadam SY,et al. The comparative evaluation of enzyme linked immunosorbent assay(ELISA),indirect hemagglutination test(IHA)and indirect fluorescent antibody test(IFAT)in the diagnosis of cystic echinococcosis[J].Turkiye Parazitol Derg,2009,33(1):73-76.
[32]朱兵,徐明谦,温浩,等.包虫病人唾液特异性抗体的检测研究与应用[J].中国人兽共患病学报,2001,17(3):55-57.
[33] Ravinder P,Parija SK. Urinary hydatid antigen detection by coagglutination,a cost-effective and rapid test for diagnosis of cystic echinococcosis in a rural or field setting[J]. J Clin Microbiol,2000,38(8):2972.
[34] Otero-Abad B,Armua-Fernandez MT,Deplazes P,et al. Latent class models for Echinococcus multilocularis diagnosis in foxes in Switzerland in the absence of a gold standard[J].Parasites Vectors,2017,10(1):612.
[35] Shariff M,Parija SC. Co-agglutination(Co-A)test for circulating antigen in hydatid disease[J]. J Med Microbiol,1993,38(6):391-394.
[36] Azazy AA,Abdelhamid AH. Indirect haemagglutination(IHA)for the diagnosis of hydatid disease in Yemen[J]. J Egypt Soc Parasitol,2000,30(2):407-411.
[37] Devi CS,Parija SC. Latex agglutination test(LAT)for antigen detection in the cystic fluid for the diagnosis of cystic echinococcosis[J]. Diagn Microbiol Infect Dis,2003,45(2):123-126.
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