不同基质、插穗和生根剂对巴山榧树扦插生根的影响
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摘要
为探讨不同处理对巴山榧树扦插生根的影响,分别研究了基质种类、插穗类型、生根剂不同质量浓度配比及处理时间对巴山榧树扦插生根的影响。结果表明,不同基质对巴山榧树插穗的生根率、生根数和根长的影响极显著,1年生枝条插穗经1 000 mg/L ABT1处理30 min后,扦插于V(泥炭土)∶V(蛭石)∶V(园土)=3∶1∶1混合基质中的生根率最高,达51.13%;不同插穗类型对生根率和生根数的影响差异达到极显著水平,1年生插穗的生根率明显高于2年生插穗,3年生插穗不生根,2年生插穗的生根数略高于1年生插穗;正交试验显示处理时间和ABT1浓度2个试验因素对巴山榧树插穗生根率的影响达到显著水平,IBA浓度对生根率的影响则不显著,经1 000 mg/L ABT1和200 mg/L IBA处理40 min的插穗生根效果最好,生根率达75.56%。该试验为巴山榧树扦插育苗技术的深入研究提供了参考依据,为巴山榧树快速繁育和推广应用提供了科学依据。
In order to investigate the effects of different treatments on rooting of Torreya fargesii cuttings, the research studied the effects of medium types, cutting types, different concentration ratio of rooting agents and processing time on rooting of T. fargesii cuttings. The results showed that different medium types had extremely significant effect on rooting rate, rooting numbers and root length of the cuttings of T. fargesii. There was a highest rooting rate of 51.13% when the cuttings of one-year-old branches were treated with 1 000 mg/L ABT1 for 30 min and then cut into mixed medium of V(peat soil)∶V(vermiculite)∶V(garden soil) at the ratio of 3∶1∶1. The effects of different cutting types on rooting rate and rooting number reached extremely significant level. The rooting rate of one-year-old cuttings was significantly higher than that of two-year-old cuttings. The three-year-old cuttings did not take root, and the rooting number of two-year-old cuttings was slightly higher than that of one-year-old cuttings. The orthogonal test showed that the effects of the processing time and ABT1 concentration on rooting rate of T. fargesii cuttings reached significant level, but the influence of IBA concentration on rooting rate was not significant. After the treatments of 1 000 mg/L ABT1 and 200 mg/L IBA for 40 min, the rooting effect of the cuttings was the best, and the rooting rate reached 75.56%. The experimental results might provide a reference forthe further research of the cuttage seedling technique of T. fargesii, and laid foundation for the rapid propagation and application of T. fargesii.
In order to investigate the effects of different treatments on rooting of Torreya fargesii cuttings, the research studied the effects of medium types, cutting types, different concentration ratio of rooting agents and processing time on rooting of T. fargesii cuttings. The results showed that different medium types had extremely significant effect on rooting rate, rooting numbers and root length of the cuttings of T. fargesii. There was a highest rooting rate of 51.13% when the cuttings of one-year-old branches were treated with 1 000 mg/L ABT1 for 30 min and then cut into mixed medium of V(peat soil)∶V(vermiculite)∶V(garden soil) at the ratio of 3∶1∶1. The effects of different cutting types on rooting rate and rooting number reached extremely significant level. The rooting rate of one-year-old cuttings was significantly higher than that of two-year-old cuttings. The three-year-old cuttings did not take root, and the rooting number of two-year-old cuttings was slightly higher than that of one-year-old cuttings. The orthogonal test showed that the effects of the processing time and ABT1 concentration on rooting rate of T. fargesii cuttings reached significant level, but the influence of IBA concentration on rooting rate was not significant. After the treatments of 1 000 mg/L ABT1 and 200 mg/L IBA for 40 min, the rooting effect of the cuttings was the best, and the rooting rate reached 75.56%. The experimental results might provide a reference forthe further research of the cuttage seedling technique of T. fargesii, and laid foundation for the rapid propagation and application of T. fargesii.
引文
Chai S.F.,Shi Y.C.,Chen Z.Y.,Wang D.R.,Wen X.Y.,and Ning S.J.,2012,Study on cutting propagation of the endangered plant Camellia pubipetala,Zhongzi(Seed),31(6):118-121(柴胜丰,史艳财,陈宗游,王代容,文香英,宁世江,2012,珍稀濒危植物毛瓣金花茶扦插繁殖技术研究,种子,31(6):118-121)
Dai X.Y.,Ren Z.H.,Lin Z.X.,Shi Z.L.,and Fu B.K.,2013,Study on the cuttings propagation technology of Cephalotaxus oliveri Mast.,Zhongzi(Seed),32(7):123-126(戴晓勇,任朝辉,林泽信,石志玲,付邦奎,2013,篦子三尖杉的扦插繁殖研究,种子,32(7):123-126)
Guo W.F.,and Meng C.L.,2011,Effects of root-inducing regulator,propagation medium and season on rooting of Betula alnoides cuttings,Linye Kexue Yanjiu(Forest Research),24(6):788-791(郭文福,蒙彩兰,2011,穗条生根剂育苗基质和季节对西南桦扦插生根的影响,林业科学研究,24(6):788-791)
He X.D.,Lu M.,and Wang H.Y.,2003,Rooting effects of substract and plant growth regulators on the cutting of Torreya nucifera Sieb.et Zucc.,Jiangsu Linye Keji(Journal of Jiangsu Forestry Science and Technology),30(1):23-24(何小弟,陆民,王海燕,2003,基质和植物生长调节剂对日本榧扦插生根影响试验,江苏林业科技,30(1):23-24)
Jiang B.,Wang J.H.,Shang J.,and Zhou X.R.,2016,Characters and species diversity of Torreya fargesii Franch.community in the Jinfo mountains,Journal of Landscape Research,8(2)71-74
Jiang B.,Zhou X.R.,Gong L.,Qin M.Y.,Sheng Y.L.,Zheng YH.,and Li Z.,2016,Research on seed characteristics and seedling growth law of Torreya fargesii,an endangered plan in China,Zhongzi(Seed),35(10):14-18(江波,周先容,龚练,秦明一,盛亚莉,郑永红,李枝,2016,巴山榧树种子特性与幼苗生长规律,种子,35(10):14-18)
Jin H.D.,Yu W.W.,Zeng Y.R.,Xiang M.Y.,Dai W.S.,and Dang W.Y.,2017,Cutting-based propagation in Torreya grandis‘Merrillii’,Zhejiang Nonglin Daxue Xuebao(Journal of Zhejiang A&F University),34(1):185-191(金侯定,喻卫武曾燕如,项美云,戴文圣,党婉誉,2017,香榧Torreya grandis‘Merrillii’的扦插繁殖,浙江农林大学学报,34(1):185-191)
Li P.,Guan Z.N.,Qiu P.Y.,Hu S.Q.,Wang G.F.,and Sun X.H.2017,The influence of different substrates and plant growth regulator to Torreya grandis‘Merrillii’cutting,Hubei Nongye Kexue(Hubei Agricultural Sciences),56(12):2294-2296(李鹏,管珍妮,求鹏英,胡绍泉,王国夫,孙小红,2017,不同基质和植物生长调节剂对香榧扦插生根的影响,湖北农业科学,56(12):2294-2296)
Li X.M.,Li C.N.,Pu Z.Y.,and Jiang Y.X.,2017,Effect of cutting propagation techniques of Camellia azalea on its rooting rate,Xinan Nongye Xuebao(Southwest China Journal of Agricultural Sciences),30(2):426-431(李先民,李春牛,卜朝阳,蒋月喜,2017,基质、促根剂及插穗对杜鹃红山茶扦插生根的影响,西南农业学报,30(2):426-431)
Ma C.L.,Fu R.,Huang X.X.,Xu H.,and Qin J.L.,2015,Cutting propagation and landscape application of Torreya yunna nensis,Lvse Keji(Journal of Green Science and Technology)(10):84-85(马长乐,符荣,黄晓霞,胥辉,覃家理,2015,云南榧树的扦插繁殖及园林应用,绿色科技,(10):84-85)
Ma J.,Chen F.B.,Zhou X.R.,Wang J.H.,and Song X.H.,2014Sequences analysis and comparison of trn L-trn F of geographical populations of Torreya fargesii(Taxaceae)and its closely related species,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),33(5):1053-1058(马俊陈发波,周先容,汪建华,宋晓宏,2014,不同地理种群巴山榧树及近缘种叶绿体trn L-trn F序列的分析,基因组学与应用生物学,33(5):1053-1058)
Su Z.N.,Chen S.W.,Yang J.H.,Tan L.,Luo Y.Z.,and Yang M.2013,Effect of rooting agents on root morphology of cuttings of Chinese fir clone,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),32(6):787-794(苏治南,陈诗文,杨家鸿,谭玲,罗扬卓,杨梅,2013,不同生根剂对杉木扦插根形态建成的影响,基因组学与应用生物学,32(6):787-794)
Wang J.H.,Zhou X.R.,Shang J.,Jiang B.,and Yang L.P.,2014a Niche characteristics of dominant woody plant populations in a Torreya fargesii shrub community in the Jinfo Mountains,Shengtaixue Zazhi(Chinese Journal of Ecology),33(5):1135-1141(汪建华,周先容,尚进,江波,杨利平,2014a,金佛山巴山榧树灌丛群落主要木本植物种群生态位特征,生态学杂志,33(5):1135-1141)
Wang J.H.,Zhou X.R.,Shang J.,Zhang Y.,and He X.L.,2014b,Characteristics of the Torreya fargesii community of the karst mountain shrub in the Jinfo Mountains,Sichuan Shifan Daxue Xuebao(Journal of Sichuan Normal University(Natural Science)),37(3):389-395(汪建华,周先容,尚进,江波,张燕,何秀利,2014b,金佛山喀斯特山地灌丛巴山榧树群落特征,四川师范大学学报(自然科学版),37(3):389-395)
Xie X.L.,Jiang B.,Zhou X.R.,Zhao L.X.,Wang J.H.,and Shang J.,2016,Allelopathic effects of aqueous extract of Torreya fargesii aril on seed germination and seedling growth of radish,mustard and cabbage,Agr.Biot.,5(4):11-17
Zhou X.R.,Shang J.,Chen F.B.,Song X.H.,and Jiang B.,2015,Psb A-trn H sequence analysis of Torreya fargesii and its closely related species,Xibu Linye Kexue(Journal of West China Forestry Science),44(1):16-21(周先容,尚进,陈发波,宋晓宏,江波,2015,巴山榧树及近缘种的psb A-trn H序列分析,西部林业科学,44(1):16-21)
Zhou X.R.,Wang J.H.,Shang J.,Jiang B.,and Wang X.,2016,Interspecific relationships among dominant shrub populations of Torreya fargesii shrub community in karst mountain area of Jinfo mountains,Xinan Daxue Xuebao(Journal of Southwest University(Natural Science Edition)),38(9):88-94(周先容,汪建华,尚进,江波,王霞,2016,金佛山喀斯特山地巴山榧树灌丛群落优势灌木种群的种间关系,西南大学学报(自然科学版),38(9):88-94)
Zhou X.R.,Yu Y.,Zhou S.D.,and He X.J.,2012a,Geographic distribution and potential distribution of Torreya fargesii,Linye Kexue(Scientia Silvae Sinicae),48(2):1-8(周先容,余岩,周颂东,何兴金,2012a,巴山榧树地理分布格局及潜在分布区,林业科学,48(2):1-8)
Zhou X.R.,Zhang W.,He X.J.,Zhou S.D.,Yu Y.,and Wang J.H.,2012b,Resources and conservation of Torreya fargesii,Dongbei Linye Daxue Xuebao(Journal of Northeast Forestry University),40(2):42-46(周先容,张薇,何兴金,周颂东,余岩,汪建华,2012b,巴山榧树(Torreya fargesii)资源及其保护,东北林业大学学报,40(2):42-46)
Zhou X.R.,Zhou S.D.,He X.J.,Shang J.,and Jiang B.,2011,RAPD analysis of genetic diversity of Torreya fargesii in Daloushan mountains,Dongbei Linye Daxue Xuebao(Journal of Northeast Forestry University),39(2):24-27(周先容,周颂东,何兴金,尚进,江波,2011,大娄山区巴山榧树遗传多样性的RAPD分析,东北林业大学学报,39(2):24-27)
Zhou Z.G.,Liu G.H.,Qiu R.S.,Yang L.X.,Shi X.G.,and Hu S.,2011,Study on rooting capability of softwood cuttings of endangered and endemic Tetraena mongolica Maxim.,Zhongzi(Seed),30(12):21-25(周志刚,刘果厚,邱润生,杨利祥,史学刚,扈顺,2011,珍稀濒危植物四合木嫩枝扦插生根特性的研究,种子,30(12):21-25)
Dai X.Y.,Ren Z.H.,Lin Z.X.,Shi Z.L.,and Fu B.K.,2013,Study on the cuttings propagation technology of Cephalotaxus oliveri Mast.,Zhongzi(Seed),32(7):123-126(戴晓勇,任朝辉,林泽信,石志玲,付邦奎,2013,篦子三尖杉的扦插繁殖研究,种子,32(7):123-126)
Guo W.F.,and Meng C.L.,2011,Effects of root-inducing regulator,propagation medium and season on rooting of Betula alnoides cuttings,Linye Kexue Yanjiu(Forest Research),24(6):788-791(郭文福,蒙彩兰,2011,穗条生根剂育苗基质和季节对西南桦扦插生根的影响,林业科学研究,24(6):788-791)
He X.D.,Lu M.,and Wang H.Y.,2003,Rooting effects of substract and plant growth regulators on the cutting of Torreya nucifera Sieb.et Zucc.,Jiangsu Linye Keji(Journal of Jiangsu Forestry Science and Technology),30(1):23-24(何小弟,陆民,王海燕,2003,基质和植物生长调节剂对日本榧扦插生根影响试验,江苏林业科技,30(1):23-24)
Jiang B.,Wang J.H.,Shang J.,and Zhou X.R.,2016,Characters and species diversity of Torreya fargesii Franch.community in the Jinfo mountains,Journal of Landscape Research,8(2)71-74
Jiang B.,Zhou X.R.,Gong L.,Qin M.Y.,Sheng Y.L.,Zheng YH.,and Li Z.,2016,Research on seed characteristics and seedling growth law of Torreya fargesii,an endangered plan in China,Zhongzi(Seed),35(10):14-18(江波,周先容,龚练,秦明一,盛亚莉,郑永红,李枝,2016,巴山榧树种子特性与幼苗生长规律,种子,35(10):14-18)
Jin H.D.,Yu W.W.,Zeng Y.R.,Xiang M.Y.,Dai W.S.,and Dang W.Y.,2017,Cutting-based propagation in Torreya grandis‘Merrillii’,Zhejiang Nonglin Daxue Xuebao(Journal of Zhejiang A&F University),34(1):185-191(金侯定,喻卫武曾燕如,项美云,戴文圣,党婉誉,2017,香榧Torreya grandis‘Merrillii’的扦插繁殖,浙江农林大学学报,34(1):185-191)
Li P.,Guan Z.N.,Qiu P.Y.,Hu S.Q.,Wang G.F.,and Sun X.H.2017,The influence of different substrates and plant growth regulator to Torreya grandis‘Merrillii’cutting,Hubei Nongye Kexue(Hubei Agricultural Sciences),56(12):2294-2296(李鹏,管珍妮,求鹏英,胡绍泉,王国夫,孙小红,2017,不同基质和植物生长调节剂对香榧扦插生根的影响,湖北农业科学,56(12):2294-2296)
Li X.M.,Li C.N.,Pu Z.Y.,and Jiang Y.X.,2017,Effect of cutting propagation techniques of Camellia azalea on its rooting rate,Xinan Nongye Xuebao(Southwest China Journal of Agricultural Sciences),30(2):426-431(李先民,李春牛,卜朝阳,蒋月喜,2017,基质、促根剂及插穗对杜鹃红山茶扦插生根的影响,西南农业学报,30(2):426-431)
Ma C.L.,Fu R.,Huang X.X.,Xu H.,and Qin J.L.,2015,Cutting propagation and landscape application of Torreya yunna nensis,Lvse Keji(Journal of Green Science and Technology)(10):84-85(马长乐,符荣,黄晓霞,胥辉,覃家理,2015,云南榧树的扦插繁殖及园林应用,绿色科技,(10):84-85)
Ma J.,Chen F.B.,Zhou X.R.,Wang J.H.,and Song X.H.,2014Sequences analysis and comparison of trn L-trn F of geographical populations of Torreya fargesii(Taxaceae)and its closely related species,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),33(5):1053-1058(马俊陈发波,周先容,汪建华,宋晓宏,2014,不同地理种群巴山榧树及近缘种叶绿体trn L-trn F序列的分析,基因组学与应用生物学,33(5):1053-1058)
Su Z.N.,Chen S.W.,Yang J.H.,Tan L.,Luo Y.Z.,and Yang M.2013,Effect of rooting agents on root morphology of cuttings of Chinese fir clone,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),32(6):787-794(苏治南,陈诗文,杨家鸿,谭玲,罗扬卓,杨梅,2013,不同生根剂对杉木扦插根形态建成的影响,基因组学与应用生物学,32(6):787-794)
Wang J.H.,Zhou X.R.,Shang J.,Jiang B.,and Yang L.P.,2014a Niche characteristics of dominant woody plant populations in a Torreya fargesii shrub community in the Jinfo Mountains,Shengtaixue Zazhi(Chinese Journal of Ecology),33(5):1135-1141(汪建华,周先容,尚进,江波,杨利平,2014a,金佛山巴山榧树灌丛群落主要木本植物种群生态位特征,生态学杂志,33(5):1135-1141)
Wang J.H.,Zhou X.R.,Shang J.,Zhang Y.,and He X.L.,2014b,Characteristics of the Torreya fargesii community of the karst mountain shrub in the Jinfo Mountains,Sichuan Shifan Daxue Xuebao(Journal of Sichuan Normal University(Natural Science)),37(3):389-395(汪建华,周先容,尚进,江波,张燕,何秀利,2014b,金佛山喀斯特山地灌丛巴山榧树群落特征,四川师范大学学报(自然科学版),37(3):389-395)
Xie X.L.,Jiang B.,Zhou X.R.,Zhao L.X.,Wang J.H.,and Shang J.,2016,Allelopathic effects of aqueous extract of Torreya fargesii aril on seed germination and seedling growth of radish,mustard and cabbage,Agr.Biot.,5(4):11-17
Zhou X.R.,Shang J.,Chen F.B.,Song X.H.,and Jiang B.,2015,Psb A-trn H sequence analysis of Torreya fargesii and its closely related species,Xibu Linye Kexue(Journal of West China Forestry Science),44(1):16-21(周先容,尚进,陈发波,宋晓宏,江波,2015,巴山榧树及近缘种的psb A-trn H序列分析,西部林业科学,44(1):16-21)
Zhou X.R.,Wang J.H.,Shang J.,Jiang B.,and Wang X.,2016,Interspecific relationships among dominant shrub populations of Torreya fargesii shrub community in karst mountain area of Jinfo mountains,Xinan Daxue Xuebao(Journal of Southwest University(Natural Science Edition)),38(9):88-94(周先容,汪建华,尚进,江波,王霞,2016,金佛山喀斯特山地巴山榧树灌丛群落优势灌木种群的种间关系,西南大学学报(自然科学版),38(9):88-94)
Zhou X.R.,Yu Y.,Zhou S.D.,and He X.J.,2012a,Geographic distribution and potential distribution of Torreya fargesii,Linye Kexue(Scientia Silvae Sinicae),48(2):1-8(周先容,余岩,周颂东,何兴金,2012a,巴山榧树地理分布格局及潜在分布区,林业科学,48(2):1-8)
Zhou X.R.,Zhang W.,He X.J.,Zhou S.D.,Yu Y.,and Wang J.H.,2012b,Resources and conservation of Torreya fargesii,Dongbei Linye Daxue Xuebao(Journal of Northeast Forestry University),40(2):42-46(周先容,张薇,何兴金,周颂东,余岩,汪建华,2012b,巴山榧树(Torreya fargesii)资源及其保护,东北林业大学学报,40(2):42-46)
Zhou X.R.,Zhou S.D.,He X.J.,Shang J.,and Jiang B.,2011,RAPD analysis of genetic diversity of Torreya fargesii in Daloushan mountains,Dongbei Linye Daxue Xuebao(Journal of Northeast Forestry University),39(2):24-27(周先容,周颂东,何兴金,尚进,江波,2011,大娄山区巴山榧树遗传多样性的RAPD分析,东北林业大学学报,39(2):24-27)
Zhou Z.G.,Liu G.H.,Qiu R.S.,Yang L.X.,Shi X.G.,and Hu S.,2011,Study on rooting capability of softwood cuttings of endangered and endemic Tetraena mongolica Maxim.,Zhongzi(Seed),30(12):21-25(周志刚,刘果厚,邱润生,杨利祥,史学刚,扈顺,2011,珍稀濒危植物四合木嫩枝扦插生根特性的研究,种子,30(12):21-25)