可降解镁合金AZ31组织夹在新西兰兔体内的实验研究
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摘要
目的研究镁合金AZ31组织夹在生物体内的降解情况及其对动物各脏器功能的影响。方法建立镁合金AZ31组织夹植入新西兰兔腹腔模型,定时观察伤口愈合及一般状况。检测血常规、凝血功能、肝肾功能及钙磷镁等,观察材料植入后上述指标的变化。收集心、肝、脾、肺、肾及脑等组织标本,行HE染色,对各脏器进行组织学观察。通过X线摄片观察组织夹在新西兰兔体内的降解情况。结果血液学检测结果显示,镁合金AZ31组织夹对血常规各项指标、凝血功能、肝肾功能等均无明显影响(P> 0.05)。重要内脏器官组织切片HE染色光镜下未见有害物质集聚。X线片显示,组织夹在新西兰兔体内发生缓慢降解。结论镁合金AZ31是较为安全的医用可降解镁合金材料,用其制作的组织夹具有一定的临床应用前景。
Objective To study the degradation of magnesium alloy AZ31 tissue clips and its effect on the function of animal organs. Methods New Zealand rabbits were used to build the magnesium alloy AZ31 implanted model. Regular observation of wound healing and general condition was firstly carried out. And then blood was collected to detect the blood routine, coagulation function, liver and kidney functions and the content of calcium,phosphate and magnesium according to the clinical testing methods. The heart, liver, spleen, lung, kidney samples were collected to examine whether pathological changes contained in them. X-ray was carried out to observe the degradation of tissue clips in New Zealand rabbits. Results The biological safety of AZ31 material was confirmed by safety research in vivo. This material had no effect on the results of blood routine index, coagulation function,hepatic function, and renal function cardiac function. No accumulation of harmful substances was observed under the light microscope after HE staining for tissue sections of important internal organs. X-rays showed that there was no significant gas accumulated around the clips and tissue clips degrade slowly in New Zealand rabbitss.Conlusion Magnesium alloy AZ31 is a relatively safe degradable magnesium alloy material, which can be used to make tissue clips, and has a certain clinical application prospect.
Objective To study the degradation of magnesium alloy AZ31 tissue clips and its effect on the function of animal organs. Methods New Zealand rabbits were used to build the magnesium alloy AZ31 implanted model. Regular observation of wound healing and general condition was firstly carried out. And then blood was collected to detect the blood routine, coagulation function, liver and kidney functions and the content of calcium,phosphate and magnesium according to the clinical testing methods. The heart, liver, spleen, lung, kidney samples were collected to examine whether pathological changes contained in them. X-ray was carried out to observe the degradation of tissue clips in New Zealand rabbits. Results The biological safety of AZ31 material was confirmed by safety research in vivo. This material had no effect on the results of blood routine index, coagulation function,hepatic function, and renal function cardiac function. No accumulation of harmful substances was observed under the light microscope after HE staining for tissue sections of important internal organs. X-rays showed that there was no significant gas accumulated around the clips and tissue clips degrade slowly in New Zealand rabbitss.Conlusion Magnesium alloy AZ31 is a relatively safe degradable magnesium alloy material, which can be used to make tissue clips, and has a certain clinical application prospect.
引文
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[2]BOZKURT M A,NSAL M G,KAPAN S,et al. Two different methods for appendiceal stump closure:metal clip and Hem-o-lok clip[J].Journal of Laparoendoscopic&Advanced Surgical Techniques Part A,2014,24(8):571.
[3]LV B,ZHANG X,LI J,et al. Absorbable polymeric surgical clips for appendicular stump closure:A randomized control trial of laparoscopic appendectomy with lapro-clips[J].Oncotarget,2016,7(27):41265-41273.
[4]HAGHSHENAS M.Mechanical characteristics of biodegradable magnesium matrix composites:A review[J].Journal of Magnesium&Alloys,2017,5(2):189-201.
[5]宋融.生物可降解腹腔镜手术止血夹用镁合金的研究[D].天津:天津理工大学,2015.
[6]MOCHIZUKI A,YAHATA C,TAKAI H. Cytocompatibility of magnesium and AZ31 alloy with three types of cell lines using a direct in vitro method[J].Journal of Materials Science Materials in Medicine,2016,27(9):145.
[7]奚廷斐,魏利娜,刘婧,等.镁合金全降解血管支架研究进展[J].金属学报,2017,53(10):1153-1167.
[8]顾炜,蔡兵,吴鸣宇. Hem-o-lok夹和可吸收夹在腹腔镜胆囊切除术中的临床应用比较[J].肝胆胰外科杂志,2015,27(3):238-240.
[9]焦鹤仙.经胃镜金属钛夹治疗上消化道血管畸形出血的护理[J].昆明医科大学学报,2017,38(2):141-144.
[10]张凯恋,张芬,林菲,等.右乳癌保乳术后X线瘤床同步整合补量调强放疗的剂量学研究[J].吉林医学,2016,37(4):816-819.
[11]HU W,LI Y.Replacement of titanium cilps by absorbable sutures in laparoscopic cholecystectomy[J].Chinese Journal of Minimally Invasive Surgery,2010,10(5):429-430.
[12]ZHANG K L,LIN F,ZHANG F,et al. Dosim etry study of silver clip marker on simultaneous integrated boost intensity modulated radiotherapy for right breast after breast-conserving surgery[J].Biomedical Engineering&Clinical Medicine,2016,20(2):157-161.
[13]SHANG X,TAO L I,YUTONG L U,et al. Effect of microenvironment changes during AZ31B magnesium alloy degradation on osteoblast proliferation and adhesion in vitro[J].Chinese Journal of Medical Physics,2017,34(9):946-949.
[14]BAI N,TAN C,LI Q,et al. Study on the corrosion resistance and anti-infection of modified magnesium alloy[J].Bio-Medical Materials and Engineering,2017,28(4):339.
[15]ERNSTBERGER T,BUCHHORN G,HEIDRICH G.Magnetic resonance imaging evaluation of intervertebral test spacers:an experimental comparison of magnesium versus titanium and carbon fiber reinforced polymers as biomaterials[J].Irish Journal of Medical Science,2010,179(1):107-111.
[16]LIN W,ZOU N,LI Q Z. Mechanical and biodegradable behavior of AZ31 magnesium alloy immersed in simulated body fluid[J].Challenges in Mechanics of Time Dependent Materials,2018,2:49-55.
[17]张慧娟,马林.乳腺癌术后全乳腺或胸壁外照射大分割放射治疗的临床研究进展[J].解放军医学院学报,2016,37(6):661-664.
[18]WITTE F,FISCHER J,NELLESEN J,et al. In vitro and in vivo corrosion measurements of magnesium alloys[J].Biomaterials,2006,27(7):1013-1018.
[19]高家诚,胡德,宋长江.医用镁合金降解及其对人体的影响[J].功能材料,2012,43(19):2577-2583.