“东海1号”大黄鱼选育F_4代遗传多样性的AFLP分析
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摘要
应用扩增片段长度多态性(AFLP)技术对以岱衢洋野生大黄鱼为亲本、通过群体选育方法获得的"东海1号"大黄鱼选育系F4代进行了遗传多样性分析.结果显示:16对AFLP选择性扩增引物组合在选育F4代的20尾个体中共扩增出736个位点,其中多态位点292个,多态率为39.67%;各引物对的Nei’s遗传多样性指数范围为0.090 4~0.203 7,平均值为0.139 9;Shannon多样性范围为0.132 0~0.304 7,平均值为0.209 9;其遗传多样性水平与原始亲本群体相当.表明若群体选育方法得当,多代选育后仍能维持较好的遗传多样性水平.
Amplified fragment length polymorphism(AFLP) technique was used to analyze the genetic diversities of the 4 th generation(F4) of "Donghai No.1" large yellow croaker(Larimichthys crocea) breeding line. The breeding line was derived from the wild large yellow croaker that captured from Daiquyang in Zhejiang Province. Using 16 AFLP selective primer-pairs, a total of 736 loci were detected from 20 individuals, of which 292 loci were polymorphic. The percentage of polymorphism was 39.67%. The range of Nei's genetic diversity and Shannon genetic diversity index were 0.090 4-0.203 7(mean 0.139 9) and 0.132 0-0.304 7(mean 0.209 9), respectively. The genetic diversity of F4 was at the similar level compared to original parents. It indicates that the genetic diversity would not decrease significantly after several generations with proper mass selection method.
Amplified fragment length polymorphism(AFLP) technique was used to analyze the genetic diversities of the 4 th generation(F4) of "Donghai No.1" large yellow croaker(Larimichthys crocea) breeding line. The breeding line was derived from the wild large yellow croaker that captured from Daiquyang in Zhejiang Province. Using 16 AFLP selective primer-pairs, a total of 736 loci were detected from 20 individuals, of which 292 loci were polymorphic. The percentage of polymorphism was 39.67%. The range of Nei's genetic diversity and Shannon genetic diversity index were 0.090 4-0.203 7(mean 0.139 9) and 0.132 0-0.304 7(mean 0.209 9), respectively. The genetic diversity of F4 was at the similar level compared to original parents. It indicates that the genetic diversity would not decrease significantly after several generations with proper mass selection method.
引文
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[18]钟立强,张成峰,周凯,等.四个鲤鱼种群遗传多样性的AFLP分析[J].基因组学与应用生物学,2010,29(2):259-265.
[19]刘必谦,董闻琦,王亚军,等.岱衢族大黄鱼种质的AFLP分析[J].水生生物学报,2005,29(4):413-416.
[20]张辛,许璞,许广平,等.中国东海日本鳗鲡种群遗传结构的AFLP分析[J].水生态学杂志,2010,3(1):82-86.
[21]张丽艳,苏永全,丁少雄,等.福建近海蓝圆鲹种群遗传多样性的AFLP分析[J].水产学报,2010,34(5):680-687.
[22]黎中宝,雷光高,常建波,等.半滑舌鳎(Cynoglossus semilaevis)野生与养殖群体遗传多样性的比较研究[J].海洋与湖沼,2011,42(3):414-418.
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[27]Templeton A R,Read B.Elimination of inbreeding depression from a captive population of Speke’s gazelle:Validity of the original statistical analysis and confirmation by permutation testing[J].Zoo Biology,1998,17:77-94.
[28]李明云,苗亮,陈炯,等.大黄鱼仿生态分阶段养殖新模式的构建及问题探讨[J].宁波大学学报(理工版),2017,30(2):1-5.
[2]王军,全成干,苏永全,等.官井洋大黄鱼遗传多样性的RAPD分析[J].海洋学报,2001,23(3):87-91.
[3]刘家富,韩坤煌.我国大黄鱼产业的发展现状与对策[J].福建水产,2011,33(5):4-8.
[4]黎中宝,方秀,陈锦,等.大黄鱼(Pseudosciaena crocea)养殖群体遗传多样性的降低[J].海洋与湖沼,2009,40(4):446-450.
[5]朱峰,石志洲,凌信文,等.宁德三沙湾网箱养殖与环境质量的关系[J].海洋通报,2013,32(2):171-177.
[6]赵广泰,刘贤德,王志勇,等.大黄鱼连续4代选育群体遗传多样性与遗传结构的微卫星分析[J].水产学报,2010,34(4):500-507.
[7]李明云,苗亮,陈炯,等.基于种群生态学概念论大黄鱼种群的划分[J].宁波大学学报(理工版),2013,26(1):1-5.
[8]苗亮,李明云,陈炯,等.快长、耐低温大黄鱼新品种东海1号的选育[J].农业生物技术学报,2014,22(10):1314-1320.
[9]丁诗华,黄丽英,张海琪,等.大黄鱼(Pseudosciaena crocea)岱衢洋选育群体和官井洋养殖群体的遗传差异分析[J].海洋与湖沼,2006,37(1):41-46.
[10]范慧慧,李明云.香鱼基因组DNA提取方法的优化[J].生物技术通报,2012,28(5):158-162.
[11]Wang Z Y,Tsoi K H,Chu K H.Applications of AFLP technology in genetic and phylogenetic analysis of penaeid shrimp[J].Biochemical Systematics&Ecology,2004,32(4):399-407.
[12]Nei M,Li W H.Mathematical model for studying genetic variation in terms of restriction endonucleases[J].Proceedings of the National Academy of Sciences,1979,76(10):5269-5273.
[13]Vuylsteke M,Peleman J D,Eijk M J T.AFLP technology for DNA fingerprinting[J].Nature Protocols,2007,2(6):1387-1398.
[14]王志勇,王艺磊,林利民,等.福建官井洋大黄鱼AFLP指纹多态性的研究[J].中国水产科学,2002,9(3):198-202.
[15]Nomura K,Ozaki A,Morishima K,et al.A genetic linkage map of the Japanese eel(Anguilla japonica)based on AFLP and microsatellite markers[J].Aquaculture,2011,310(3/4):329-342.
[16]Bonin A,Ehrich D,Manel S.Statistical analysis of amplified fragment length polymorphism data:A toolbox for molecular ecologists and evolutionists[J].Molecular Ecology,2007,16(18):3737-3758.
[17]松松,苗亮,李明云,等.香鱼(Plecoglossus altivelis)养殖群体遗传多样性的AFLP分析及性别特异性分子标记筛选[J].海洋与湖沼,2014,45(2):395-399.
[18]钟立强,张成峰,周凯,等.四个鲤鱼种群遗传多样性的AFLP分析[J].基因组学与应用生物学,2010,29(2):259-265.
[19]刘必谦,董闻琦,王亚军,等.岱衢族大黄鱼种质的AFLP分析[J].水生生物学报,2005,29(4):413-416.
[20]张辛,许璞,许广平,等.中国东海日本鳗鲡种群遗传结构的AFLP分析[J].水生态学杂志,2010,3(1):82-86.
[21]张丽艳,苏永全,丁少雄,等.福建近海蓝圆鲹种群遗传多样性的AFLP分析[J].水产学报,2010,34(5):680-687.
[22]黎中宝,雷光高,常建波,等.半滑舌鳎(Cynoglossus semilaevis)野生与养殖群体遗传多样性的比较研究[J].海洋与湖沼,2011,42(3):414-418.
[23]郭宏波.野生与栽培党参遗传多样性及其保存和保护策略研究[D].上海:复旦大学,2007.
[24]Basiao Z U,Taniguchi N.An investigation of enzyme and other protein polymorphisms in Japanese stock of the tilapias Oreochromis niloticus and Tilapia zilli[J].Aquaculture,1984,38:335-345.
[25]董闻琦.浙江海区黄鱼属种群的遗传变异和种质研究[D].宁波:宁波大学,2003.
[26]Templeton A R,Read B.Factors eliminating inbreeding depression in a captive herd of Speke’s gazelle(Gazella spekei)[J].Zoo Biology,1984,3:177-199.
[27]Templeton A R,Read B.Elimination of inbreeding depression from a captive population of Speke’s gazelle:Validity of the original statistical analysis and confirmation by permutation testing[J].Zoo Biology,1998,17:77-94.
[28]李明云,苗亮,陈炯,等.大黄鱼仿生态分阶段养殖新模式的构建及问题探讨[J].宁波大学学报(理工版),2017,30(2):1-5.