中药材衢枳壳的基源植物研究
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摘要
目的厘清中药材衢枳壳基源植物的起源,解决衢枳壳基源植物起源的争议,根据各种文献使用的不同名称,确定科学的中文名和拉丁学名正名。方法对衢枳壳形态、栽培历史和抗寒基因特性进行调查研究,对PCR扩增、化学成分和基因片段测序等方面进行文献考证。结果衢枳壳基源植物的形态、PCR扩增结果和ITS1测序结果均与柚和酸橙非常相似或相同,而化学成分和抗寒基因特性与酸橙十分相近。结论衢枳壳的基源植物系柚和酸橙的自然杂交栽培变种,不具备独立种的地位,其拉丁学名应为Citrus aurantium ‘Changshan-huyou’,中文名宜为常山柚橙,常山胡柚和胡柚可作为别名。
OBJECTIVE To clarify the plant origin of the Chinese medicinal materials Qu Aurantii Fructus and solve the controversy about the original plant of Qu Aurantii Fructus, determine its scientific Chinese name and Latin names from different names which used in various literature. METHODS Making an investigation of the morphology, culture history and characteristics of cold-resistant genes, and literature textual research on PCR amplification, chemical composition and gene fragment sequencing of the original plant. RESULTS The morphology, PCR amplification and ITS1 sequencing of the Qu Aurantii Fructus were same or very similar to those of Citrus grandis and Citrus aurantium. The chemical composition and cold-resistance genes were very similar to those of Citrus aurantium. CONCLUSION It is determined that the original plant of Qu Aurantii Fructus is a natural hybrid cultivated variety of Citrus grandis and Citrus aurantium, which does not have the status of independent species of plant. The original plant Latin name and Chinese name of the Qu Aurantii Fructus are Citrus aurantium‘Changshan-huyou' and Changshan Youcheng respectively. Changshan Huyou and Huyou can be used as synonymy.
OBJECTIVE To clarify the plant origin of the Chinese medicinal materials Qu Aurantii Fructus and solve the controversy about the original plant of Qu Aurantii Fructus, determine its scientific Chinese name and Latin names from different names which used in various literature. METHODS Making an investigation of the morphology, culture history and characteristics of cold-resistant genes, and literature textual research on PCR amplification, chemical composition and gene fragment sequencing of the original plant. RESULTS The morphology, PCR amplification and ITS1 sequencing of the Qu Aurantii Fructus were same or very similar to those of Citrus grandis and Citrus aurantium. The chemical composition and cold-resistance genes were very similar to those of Citrus aurantium. CONCLUSION It is determined that the original plant of Qu Aurantii Fructus is a natural hybrid cultivated variety of Citrus grandis and Citrus aurantium, which does not have the status of independent species of plant. The original plant Latin name and Chinese name of the Qu Aurantii Fructus are Citrus aurantium‘Changshan-huyou' and Changshan Youcheng respectively. Changshan Huyou and Huyou can be used as synonymy.
引文
[1]浙江省中药炮制规范[S].2015:180-182.
[2]吴耕民.金柚特性及其栽培技术要点[J].中国柑桔,1987,16(1):16-18.
[3]蔡剑华,贺善安,缪天纲,等.珍贵的柑桔种质资源[C]//张上隆主编.纪念吴耕民教授诞辰一百周年论文集.北京:中国农业科技出版社,1995:18-22.
[4]贝增明,叶杏元.我国杂柑良种常山柚橙简介[C]//纪念吴耕民教授诞辰一百周年论文集.北京:中国农业科技出版社,1995:23-24.
[5]CHEN L G,HU X Q,ZHAO S Q.Molecular research on Huyou origin[J].Acta Horticu Sinica(园艺学报),2002,29(3):276-277.
[6]ZHANG Y B.A new species of genus Citrus from China[J].Bull Bot Res,1991,11(2):5-7.
[7]XU C J,BAO L,ZHANG B,et al.Parentage analysis of Huyou(Citrus changshanensis)based on internal transcribed spacer sequences[J].Plant Breeding,2006(125):519-522.
[8]ZHAO W L,GUO Z X,ZHANG W T,et al.Study on original plant species and geographical distribution of Fructus Aurantii[J].Chin J Chin Meter Med(中国中药杂志),2018,43(21):4361-4364.
[9]ZHAO X M,YE X Q,ZHU D Y.Isolation and identification of chemical constituents from peels of Citrus changshan-huyou Y.B.Chang[J].Beijing Da Xue Xue Bao,2009,41(41):575-577.
[10]ZHANG Y Y,YE W C,FAN C L,et al.A New coumarin glucoside from the fruits of Citrus aurantium[J].Chin J Nat Med(中国天然药物),2005,3(3):141-143.
[11]DENG K Z,DING Y Q,ZHOU B,et al.Isolation and structural identification of chemical constituents from Aurantii Fructus[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2015,21(14):36-38.
[12]ZHAO X M,YE X Q,ZHU D Y.Study on the volatile oils and volatile components in the rind of Chanshan Huyou pomelo cultivar[J].J Fruit Sci(果树学报),2007,24(1):109-112.
[13]黄爱华,吴波,曾元儿,等.酸橙幼果中挥发油的GC-MS分析[J].中药材,2010,33(11):1748-1750.
[14]YUAN W B,WU Q D,WU X R.Analysis of the constituents of volatile oil from Fructus Auranti immaturus by GC-MS[J].J Chin Med Mater(中药材),2011,34(7):1067-1069.
[15]ZHAO X M,YE X Q,XI Y F,et al.Flavonoids in peels of Citrus changshanhu-you[J].Chin Tradit Herb Drug(中草药),2003,34(1):11-13.
[16]ZHOU D Y,XU Q,XUE X Y,et al.Analysis of flavonoid glycosides in Fructus Aurantii by high performance liquid chromatography-electrospray ionization mass spectrometr[J].Chin J Anal Chem(分析化学),2006,34(S1):31-35.
[17]ZHAO X M,YE X Q,XI Y F,et al.Isolation and identification of effective constituents from the peels of Citrus paradise cv.Changshanhuyou and their pharmacological activities[J].J Fruit Sci(果树学报),2006,23(3):458-461.
[18]DING Y Q,XIONG Y,ZHOU B,et al.Isolation and structural identification of flavonoids from Aurantii Fructus[J].Chin JChin Meter Med(中国中药杂志),2015,40(12):2352-2356.
[19]HUANG W K,YUE C,SONG J F,et al.Simultaneous determination of seven constituents in Citrus Changshanhuyou Y.B.Chang by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(3):404-407.
[20]LI C M,ZHONG Z H,DOU H L,et al.Separation and identification of two flavonones from peels of Citrus Changshan-huyou[J].Food Sci(食品科学),2006,27(6):161-164.
[21]ZHAO X M,YE X Q,ZHU D Y.A novel compound isolated from the peels of Citrus Changshan-huyou Y.B.Chang[J].Acta Pharmac Sinica,2008,43(12):1208-1210.
[22]MIN P,ZHAO Q C,GAO Y J,et al.The chemical constituents of Citrus changshan-huyou Y.B.Chang[J].Chin J Med Chem(中国药物化学杂志),2010,20(2):129-132.
[23]ARBO M,LARENTIS E R,LINCK V M,et al.Concentrations of p-synephrine in fruits and leaves of Citrus species(Rutaceae)and the acute toxicity testing of Citrus aurantium extract and p-synephrine[J].Food and Chemical Toxicology,2008,6(8):2770-2775.
[24]YIN L,CAO X L,XU J,et al.Preparative separation of flavonoids from Exocarpium Citri grandis by high performance countercurrent chromatography[J].Food Sci(食品科学),2013,34(20):268-272.
[25]XIANG Y,CAO J,LUO F,et al.Simultaneous from purification of limonin,nomilin and isoobacunoic acid pomelo fruit(Citrus grandis)segment membrane[J].J Food Sci(果树学报),2014,79(10):C1956-C1963.
[26]FENG B M,PEI Y H.Research on the chemical constituents of the coumarins from Citrus grandis Osbeck[J].J Shenyang Pharm Univ(沈阳药科大学学报),2000,17(4):253-255.
[27]YUAN X L,LIANG C Y,TAN Y F,et al.Analysis of the volatile oil from Citrus grandis in different habitats by GC-MS[J].China Pharmacy(中国药房),2011,22(47):4481-4483.
[28]刘联友.一九九一年柑桔冻害专题报导[J].浙江柑桔,1992(2):8-9.
[29]温明霞,林媚,聂振朋,等.胡柚及其芽变品系抗寒性研究初报[J].福建果树,2008(2):18-19.
[30]MA W T,FAN W G.Determination and comprehensive evaluation on cold-tolerance of wild Citrus from Guizhou[J].Acta Bot Boreal,34(10):2063-2069.
[31]BALDWIN B G,SANDERSON M J,PORTER J M,et al.The ITS region of nuclear ribosomal DNA:a valuable source of evidence on angiosperm phylogeny[J].Ann Mo Bot Gard,1995(82):247-277.
[2]吴耕民.金柚特性及其栽培技术要点[J].中国柑桔,1987,16(1):16-18.
[3]蔡剑华,贺善安,缪天纲,等.珍贵的柑桔种质资源[C]//张上隆主编.纪念吴耕民教授诞辰一百周年论文集.北京:中国农业科技出版社,1995:18-22.
[4]贝增明,叶杏元.我国杂柑良种常山柚橙简介[C]//纪念吴耕民教授诞辰一百周年论文集.北京:中国农业科技出版社,1995:23-24.
[5]CHEN L G,HU X Q,ZHAO S Q.Molecular research on Huyou origin[J].Acta Horticu Sinica(园艺学报),2002,29(3):276-277.
[6]ZHANG Y B.A new species of genus Citrus from China[J].Bull Bot Res,1991,11(2):5-7.
[7]XU C J,BAO L,ZHANG B,et al.Parentage analysis of Huyou(Citrus changshanensis)based on internal transcribed spacer sequences[J].Plant Breeding,2006(125):519-522.
[8]ZHAO W L,GUO Z X,ZHANG W T,et al.Study on original plant species and geographical distribution of Fructus Aurantii[J].Chin J Chin Meter Med(中国中药杂志),2018,43(21):4361-4364.
[9]ZHAO X M,YE X Q,ZHU D Y.Isolation and identification of chemical constituents from peels of Citrus changshan-huyou Y.B.Chang[J].Beijing Da Xue Xue Bao,2009,41(41):575-577.
[10]ZHANG Y Y,YE W C,FAN C L,et al.A New coumarin glucoside from the fruits of Citrus aurantium[J].Chin J Nat Med(中国天然药物),2005,3(3):141-143.
[11]DENG K Z,DING Y Q,ZHOU B,et al.Isolation and structural identification of chemical constituents from Aurantii Fructus[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2015,21(14):36-38.
[12]ZHAO X M,YE X Q,ZHU D Y.Study on the volatile oils and volatile components in the rind of Chanshan Huyou pomelo cultivar[J].J Fruit Sci(果树学报),2007,24(1):109-112.
[13]黄爱华,吴波,曾元儿,等.酸橙幼果中挥发油的GC-MS分析[J].中药材,2010,33(11):1748-1750.
[14]YUAN W B,WU Q D,WU X R.Analysis of the constituents of volatile oil from Fructus Auranti immaturus by GC-MS[J].J Chin Med Mater(中药材),2011,34(7):1067-1069.
[15]ZHAO X M,YE X Q,XI Y F,et al.Flavonoids in peels of Citrus changshanhu-you[J].Chin Tradit Herb Drug(中草药),2003,34(1):11-13.
[16]ZHOU D Y,XU Q,XUE X Y,et al.Analysis of flavonoid glycosides in Fructus Aurantii by high performance liquid chromatography-electrospray ionization mass spectrometr[J].Chin J Anal Chem(分析化学),2006,34(S1):31-35.
[17]ZHAO X M,YE X Q,XI Y F,et al.Isolation and identification of effective constituents from the peels of Citrus paradise cv.Changshanhuyou and their pharmacological activities[J].J Fruit Sci(果树学报),2006,23(3):458-461.
[18]DING Y Q,XIONG Y,ZHOU B,et al.Isolation and structural identification of flavonoids from Aurantii Fructus[J].Chin JChin Meter Med(中国中药杂志),2015,40(12):2352-2356.
[19]HUANG W K,YUE C,SONG J F,et al.Simultaneous determination of seven constituents in Citrus Changshanhuyou Y.B.Chang by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(3):404-407.
[20]LI C M,ZHONG Z H,DOU H L,et al.Separation and identification of two flavonones from peels of Citrus Changshan-huyou[J].Food Sci(食品科学),2006,27(6):161-164.
[21]ZHAO X M,YE X Q,ZHU D Y.A novel compound isolated from the peels of Citrus Changshan-huyou Y.B.Chang[J].Acta Pharmac Sinica,2008,43(12):1208-1210.
[22]MIN P,ZHAO Q C,GAO Y J,et al.The chemical constituents of Citrus changshan-huyou Y.B.Chang[J].Chin J Med Chem(中国药物化学杂志),2010,20(2):129-132.
[23]ARBO M,LARENTIS E R,LINCK V M,et al.Concentrations of p-synephrine in fruits and leaves of Citrus species(Rutaceae)and the acute toxicity testing of Citrus aurantium extract and p-synephrine[J].Food and Chemical Toxicology,2008,6(8):2770-2775.
[24]YIN L,CAO X L,XU J,et al.Preparative separation of flavonoids from Exocarpium Citri grandis by high performance countercurrent chromatography[J].Food Sci(食品科学),2013,34(20):268-272.
[25]XIANG Y,CAO J,LUO F,et al.Simultaneous from purification of limonin,nomilin and isoobacunoic acid pomelo fruit(Citrus grandis)segment membrane[J].J Food Sci(果树学报),2014,79(10):C1956-C1963.
[26]FENG B M,PEI Y H.Research on the chemical constituents of the coumarins from Citrus grandis Osbeck[J].J Shenyang Pharm Univ(沈阳药科大学学报),2000,17(4):253-255.
[27]YUAN X L,LIANG C Y,TAN Y F,et al.Analysis of the volatile oil from Citrus grandis in different habitats by GC-MS[J].China Pharmacy(中国药房),2011,22(47):4481-4483.
[28]刘联友.一九九一年柑桔冻害专题报导[J].浙江柑桔,1992(2):8-9.
[29]温明霞,林媚,聂振朋,等.胡柚及其芽变品系抗寒性研究初报[J].福建果树,2008(2):18-19.
[30]MA W T,FAN W G.Determination and comprehensive evaluation on cold-tolerance of wild Citrus from Guizhou[J].Acta Bot Boreal,34(10):2063-2069.
[31]BALDWIN B G,SANDERSON M J,PORTER J M,et al.The ITS region of nuclear ribosomal DNA:a valuable source of evidence on angiosperm phylogeny[J].Ann Mo Bot Gard,1995(82):247-277.