miRNA在自体细胞移植治疗扩张型心肌病的意义
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摘要
扩张型心肌病是一类以左心室或双心室扩大伴收缩功能障碍为特征的心肌病,本病患病率约为1/2500,预后差,是心力衰竭的第三位原因,确诊后5 a生存约50%,10 a生存约25%。心脏再同步化治疗(CRT)植入同步化治疗除颤器(CRTD)等治疗手段与单纯药物治疗可有效预防心脏性猝死、降低死亡率、改善心功能,但价格昂贵、不能根本改善预后,基因治疗尚处于研究阶段,难度较大。心脏移植是最有效的根治方法,但因供体短缺、排异反应等无法广泛开展。自体骨髓单核细胞移植治疗可实现心肌细胞再生、改善新功能,进而提高生存率。同时,miRNA与扩张型心肌病的发生转归有相应的影响,本文就miRNA在自体骨髓单核细胞移植治疗扩张型心肌病中意义进行综述。
Dilated cardiomyopathy is a kind of cardiomyopathy characterized by left ventricular or double ventricular enlargement and systolic dysfunction.The prognosis is poor and the prevalence rate is about 1/2500.It is the third cause of heart failure.The 5 year survival is about 50%,and the 10 year survival is about 25%.Cardiac resynchronization therapy(CRT) implantation of synchronous treatment defibrillator(CRTD) and other treatment methods and simple drug treatment can effectively prevent sudden cardiac death,reduce mortality,improve cardiac function,but the price is expensive,can not improve the prognosis,gene therapy is still in the study stage,it is difficult.Heart transplantation is the most effective radical treatment,but donor shortage and rejection can not be widely carried out.Autologous bone marrow mononuclear cells transplantation can achieve cardiomyocyte regeneration,improve new functions,and improve survival rate.At the same time,miRNA has a corresponding effect on the outcome of dilated cardiomyopathy.This article reviews the significance of miRNA in the treatment of dilated cardiomyopathy with autologous bone marrow mononuclear cell transplantation.
Dilated cardiomyopathy is a kind of cardiomyopathy characterized by left ventricular or double ventricular enlargement and systolic dysfunction.The prognosis is poor and the prevalence rate is about 1/2500.It is the third cause of heart failure.The 5 year survival is about 50%,and the 10 year survival is about 25%.Cardiac resynchronization therapy(CRT) implantation of synchronous treatment defibrillator(CRTD) and other treatment methods and simple drug treatment can effectively prevent sudden cardiac death,reduce mortality,improve cardiac function,but the price is expensive,can not improve the prognosis,gene therapy is still in the study stage,it is difficult.Heart transplantation is the most effective radical treatment,but donor shortage and rejection can not be widely carried out.Autologous bone marrow mononuclear cells transplantation can achieve cardiomyocyte regeneration,improve new functions,and improve survival rate.At the same time,miRNA has a corresponding effect on the outcome of dilated cardiomyopathy.This article reviews the significance of miRNA in the treatment of dilated cardiomyopathy with autologous bone marrow mononuclear cell transplantation.
引文
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[7]Zhu N,Zhang D,Xie H,et al.Endothelial-specific intronderived mi R-126is down-regulated in human breast cancer and targets both vegfa and pik3R2[J].Mol Cell Biochem,2011;351(1/2):157-164
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[9]OK jin,EJ kwon,WS dong,et al.Mir-185inhibits endoplasmic reticulum stress-induced apoptosis by targeting Na+/H+exchanger-1in the heart[J].Bmb Reports,2016;49(4):208-213
[10]K klingel,R kandolf.Update on myocarditis[J].Journal of the American College of Cardiology,2012;59(9):779-792
[11]Christian be SLEr,Daniel urban,Stefan watzka,et al.Endomyocardial mir-133a levels correlate with myocardial inflammation,Improved left ventricular function,And clinical outcome in patients with inflammatory cardiomyopathy[J].European Journal of Heart Failure,2016;18(12):1442-1451
[12]Anna wojciechowska,Agata braniewska,Katarzyna kozarkamińska.Microrna in cardiovascular biology and disease[J].Adv Clin Exp Med,2017;26(5):865-874
[13]Romaine SP,Tomaszewski M,Condorelli G,et al.MicroRnas in cardiovascular disease an introduction for clinicians[J].Heart,2015;101(12):921-928
[14]Duistersrf,Tijsenaj,Schroenb,et al.Mir-133and mirna-300regulate connective tissue growth factor:implications for aroleofimicrornas in myocardial matrix remodeling[J].Circ Res,2009;104(2):170-178
[15]Sunman H,Canpolat U,Yorgun H,et al.Association between reverse electrical remodeling and cardiac fibrosis markers in patients with cardiac resynchronization therapy[J].Turk Kardiyoloji Dernegi arsivi,2018;46(2):84-91
[16]Isbella K,Paola B,Gabriele B,et al.Long term effects of cardiac resynchronization therapy in chronic heart failure patients[J].Archives of Cardiovascular Diseases Supplements,2010;2(1):26-26
[17]武丽萍.扩张型心肌病发病机制与治疗进展[J].中国乡村医药,2017;19(24):83-84
[18]然鋆,宋云虎,胡盛寿,等.163例肥厚型梗阻性心肌病的外科治疗及疗效评价[J].中国循环杂志,2013;28(2):136-139
[19]任保军,王悦喜,阿荣,等.自体骨髓单个核细胞移植治疗心力衰竭2年随访结果[J].内蒙古医科大学学报,2012;34(5):725-732
[20]Creemers EE,Tijsen AJ,Pinto YM.Circulating micrornas:novel biomarkers and extracellular communicators in cardiovascular disease?[J].Circ Res,2012;110(3):483-495
[21]Laura moody,Hongshan He,Yuan-xiang pan,et al.Methods and novel technology for microrna quantification in colorectal cancer screening moody,et al.[J].Clinical Epigenetics,2017;9(1):119
[22]Yamada A,Horimatsu T,Okugawa Y,et al.Serum mir-21,Mir-29a,And mir-125b are promising biomarkers for the early detection of colorectal neoplasia[J].Clin Cancer Res,2015;21(18):4234-4242
[23]Krawczyk P,Powrózek T,Olesiński T,et al.Evaluation of mir-506and mir-4316expression in early and noninvasive diagnosis of colorectal cancer[J].Int J Color Dis,2017;32(7):1057-1060
[24]Erica V.Stein,David L.Duewer,Natalia farkas,Etal.Steps to achieve quantitative measurements of microrna using two step droplet digital pcr[J].Plos one,2017;12(11):e0188085
[25]Peter androvic,Lukas valihrach,Julie elling,et al.Twotailed RT-qpcr:a novel method for highly accurate mirna quantification[J].Nucleic Acids Research,2017;45(15):e144
[26]F degliangeli,P kshirsagar,V brunetti,et al.Absolute and direct microrna quantification using dna-gold nanoparticle probes[J].Journal of the American Chemical Society,2014;136(6):2264-2267
[27]Lv W,Zhao J,Situ B,et al.A target-triggered dual amplification strategy for sensitive detection of microrna[J].Biosensors&Bioelectronics,2016;83:250-255
[28]Guven B,Dudak FC,Boyaci IH,et al.Sers-based direct and sandwich assay methods for mir-21detection[J].Analyst,2014;139(5):1141-1147
[29]Hsin-neng wang,Bridget M.Crawford,et al.Multiplexed detection of microrna biomarkers using sers based inverse molecular sentinel(ims)nanoprobes[J].J Phys Chem CNanomater Interfaces,2016;120(37):21047-21050
[2]Damy T,Goode KM,Kallvikbacka-bennett A,et al.Determinants and prognostic value of pulmonary arterial pressure in patients with chronic heart failure[J].Eur Heart J,2010;31(18):2280-2290
[3]Fang JC,De marco T,Givertz MM,et al.World health organization pulmonary hypertension group2:pulmonary hypertension due to left heart disease in the adult.A summary statement from the pulmonary hypertension council of the international society for heart and lung transplantation[J].Journal of Heart&Lung Transplantation,2012;31(9):913-933
[4]杜银苹,李东野.微小RNA在心血管疾病中的研究进展[J].中华临床医师杂志,2014;8(7):1314-1318
[5]廖江铨.mi RNA在冠心病诊断和治疗中的研究进展(J).中国科学:生命科学,2015;8(45):748-754
[6]Kukreja RC,Yin C,Salloum FN.MicroRnas:new players in cardiac injury and protection[J].Mol Pharmacol,2011;80(4):558-564
[7]Zhu N,Zhang D,Xie H,et al.Endothelial-specific intronderived mi R-126is down-regulated in human breast cancer and targets both vegfa and pik3R2[J].Mol Cell Biochem,2011;351(1/2):157-164
[8]Papoutsidakis N,Deftereos S,Kaoukis A,et al.MicroRnas and the heart:small things do matter[J].Curr Top Med Chem,2013;13(2):216-230
[9]OK jin,EJ kwon,WS dong,et al.Mir-185inhibits endoplasmic reticulum stress-induced apoptosis by targeting Na+/H+exchanger-1in the heart[J].Bmb Reports,2016;49(4):208-213
[10]K klingel,R kandolf.Update on myocarditis[J].Journal of the American College of Cardiology,2012;59(9):779-792
[11]Christian be SLEr,Daniel urban,Stefan watzka,et al.Endomyocardial mir-133a levels correlate with myocardial inflammation,Improved left ventricular function,And clinical outcome in patients with inflammatory cardiomyopathy[J].European Journal of Heart Failure,2016;18(12):1442-1451
[12]Anna wojciechowska,Agata braniewska,Katarzyna kozarkamińska.Microrna in cardiovascular biology and disease[J].Adv Clin Exp Med,2017;26(5):865-874
[13]Romaine SP,Tomaszewski M,Condorelli G,et al.MicroRnas in cardiovascular disease an introduction for clinicians[J].Heart,2015;101(12):921-928
[14]Duistersrf,Tijsenaj,Schroenb,et al.Mir-133and mirna-300regulate connective tissue growth factor:implications for aroleofimicrornas in myocardial matrix remodeling[J].Circ Res,2009;104(2):170-178
[15]Sunman H,Canpolat U,Yorgun H,et al.Association between reverse electrical remodeling and cardiac fibrosis markers in patients with cardiac resynchronization therapy[J].Turk Kardiyoloji Dernegi arsivi,2018;46(2):84-91
[16]Isbella K,Paola B,Gabriele B,et al.Long term effects of cardiac resynchronization therapy in chronic heart failure patients[J].Archives of Cardiovascular Diseases Supplements,2010;2(1):26-26
[17]武丽萍.扩张型心肌病发病机制与治疗进展[J].中国乡村医药,2017;19(24):83-84
[18]然鋆,宋云虎,胡盛寿,等.163例肥厚型梗阻性心肌病的外科治疗及疗效评价[J].中国循环杂志,2013;28(2):136-139
[19]任保军,王悦喜,阿荣,等.自体骨髓单个核细胞移植治疗心力衰竭2年随访结果[J].内蒙古医科大学学报,2012;34(5):725-732
[20]Creemers EE,Tijsen AJ,Pinto YM.Circulating micrornas:novel biomarkers and extracellular communicators in cardiovascular disease?[J].Circ Res,2012;110(3):483-495
[21]Laura moody,Hongshan He,Yuan-xiang pan,et al.Methods and novel technology for microrna quantification in colorectal cancer screening moody,et al.[J].Clinical Epigenetics,2017;9(1):119
[22]Yamada A,Horimatsu T,Okugawa Y,et al.Serum mir-21,Mir-29a,And mir-125b are promising biomarkers for the early detection of colorectal neoplasia[J].Clin Cancer Res,2015;21(18):4234-4242
[23]Krawczyk P,Powrózek T,Olesiński T,et al.Evaluation of mir-506and mir-4316expression in early and noninvasive diagnosis of colorectal cancer[J].Int J Color Dis,2017;32(7):1057-1060
[24]Erica V.Stein,David L.Duewer,Natalia farkas,Etal.Steps to achieve quantitative measurements of microrna using two step droplet digital pcr[J].Plos one,2017;12(11):e0188085
[25]Peter androvic,Lukas valihrach,Julie elling,et al.Twotailed RT-qpcr:a novel method for highly accurate mirna quantification[J].Nucleic Acids Research,2017;45(15):e144
[26]F degliangeli,P kshirsagar,V brunetti,et al.Absolute and direct microrna quantification using dna-gold nanoparticle probes[J].Journal of the American Chemical Society,2014;136(6):2264-2267
[27]Lv W,Zhao J,Situ B,et al.A target-triggered dual amplification strategy for sensitive detection of microrna[J].Biosensors&Bioelectronics,2016;83:250-255
[28]Guven B,Dudak FC,Boyaci IH,et al.Sers-based direct and sandwich assay methods for mir-21detection[J].Analyst,2014;139(5):1141-1147
[29]Hsin-neng wang,Bridget M.Crawford,et al.Multiplexed detection of microrna biomarkers using sers based inverse molecular sentinel(ims)nanoprobes[J].J Phys Chem CNanomater Interfaces,2016;120(37):21047-21050