茶皂素及茶多酚对2种植物病原菌的联合毒力
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摘要
采用菌丝生长速率法测定了茶皂素及茶多酚对八角炭疽病菌及桉树梢枯病菌的联合毒力。结果表明,茶皂素和茶多酚对2种植物病原菌均具有一定的抑制作用,其中茶皂素的抑制作用均比茶多酚强。对八角炭疽病菌的室内毒力测定结果表明,EC_(50)值分别为3.72mg/mL及28.35mg/mL;对桉树梢枯病菌的室内毒力测定结果显示,EC_(50)值分别为10.02mg/mL和104.35mg/mL。茶皂素与茶多酚按照1︰1、1︰4、2︰3的比例混配后抑制八角炭疽病菌的CTC值分别为471、318、246,表现为增效作用,其中以1︰1混配后增效作用最显著;两者按照1︰1、2︰3、4︰1的比例混配后抑制桉树梢枯病菌的CTC值分别为406、141、234,也表现为增效作用,其中以1︰1混配后增效作用最显著。
Co-toxicities of tea saponin and tea polyphenol on Colletotrichum horii and Lasiodiplodia theobromae were determined by the method of mycelial growth rates. The results showed that tea saponin and tea polyphenol had inhibition effect on 2 kinds of plant pathogenic fungus,and tea saponin had higher inhibition effect than tea polyphenol,with EC_(50) of 3. 72 mg/mL and 28. 35 mg/mL on Colletotrichum horii,with EC_(50) of 10. 02 mg/mL and 104. 35 mg/mL on Lasiodiplodia theobromae. The co-toxicity(CTC) on Colletotrichum horii between tea saponin and tea polyphenol were 471,318 and 246 when their mixing ratios were 1 ︰ 1,1 ︰ 4 and 2 ︰ 3 respectively,and it showed synergistic effect. The synergistic effect was significant with 1 ︰ 1. The co-toxicity(CTC) on Lasiodiplodia theobromae between tea saponin and tea polyphenol were 406,141 and 234 when their mixing ratios were 1 ︰ 1,2 ︰ 3 and 4 ︰ 1 respectively,and it showed synergistic effect. The synergistic effect was significant with 1 ︰ 1.
Co-toxicities of tea saponin and tea polyphenol on Colletotrichum horii and Lasiodiplodia theobromae were determined by the method of mycelial growth rates. The results showed that tea saponin and tea polyphenol had inhibition effect on 2 kinds of plant pathogenic fungus,and tea saponin had higher inhibition effect than tea polyphenol,with EC_(50) of 3. 72 mg/mL and 28. 35 mg/mL on Colletotrichum horii,with EC_(50) of 10. 02 mg/mL and 104. 35 mg/mL on Lasiodiplodia theobromae. The co-toxicity(CTC) on Colletotrichum horii between tea saponin and tea polyphenol were 471,318 and 246 when their mixing ratios were 1 ︰ 1,1 ︰ 4 and 2 ︰ 3 respectively,and it showed synergistic effect. The synergistic effect was significant with 1 ︰ 1. The co-toxicity(CTC) on Lasiodiplodia theobromae between tea saponin and tea polyphenol were 406,141 and 234 when their mixing ratios were 1 ︰ 1,2 ︰ 3 and 4 ︰ 1 respectively,and it showed synergistic effect. The synergistic effect was significant with 1 ︰ 1.
引文
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[3]马锦林,曾祥艳,李开祥,等.广西八角产业现状与发展战略[J].广西林业科学,2011,40(4):336-339.
[4]刘瑞新.六万林场八角林病虫害为害结构调查初报[J].广西农学报,2015,30(6):50-53.
[5]廖旺姣,邹东霞,黄乃秀,等.一种八角炭疽病新病原鉴定[J].西南农业学报,30(10):2242-2245.
[6]黄乃秀,吴耀军,黄华艳,等.八角炭疽病菌的室内药效实验[J].广西林业科学,2004,33(4):180-181.
[7]邹东霞,廖旺姣,黄乃秀,等.8种杀菌剂对桉树枝枯病菌室内毒力测定[J].林业工程学报,2013,27(1):38-40.
[8]汪金莲,邱业先,扶教龙,等.茶多酚对稻瘟病菌的抑制作用及抑菌机理[J].天然产物研究与开发,2011,23(5):918-922.
[9]黄继光,陈秀贤,徐汉虹,等.茶皂素对12种植物病原菌的抑菌活性[J].华中农业大学学报,2013,32(2):50-53.
[10]崔宏春,周铁锋,余继忠,等.油茶皂素对茶轮斑病和茶白星病的抑菌作用[J].浙江农业科学,2012(9):1285-1287.
[11]杜宜新,石妞妞,阮宏椿,等.20种杀菌剂对芦笋茎枯病菌的抑制作用及联合毒力[J].福建农业学报,2013,28(2):143-147.
[12]张慧勤.茶皂素对两种杀菌剂抗茶树轮斑病的增效作用及其机制研究[D].武汉:华中农业大学,2011.
[13]盛书强.茶皂素对杀菌剂的增效作用及机理[D].长沙:湖南农业大学,2003.
[14]晏秀梅,魏静,许靖逸,等.茶多酚与茶皂素及其复配液的抑菌作用研究[J].食品工业科技,2014,35(22):159-161,171.
[15]冯建国,张小军,于迟,等.我国农药剂型应用研究概况[J].中国农业大学学报,2013,18(2):220-226.