扶正抑瘤汤对人肝癌细胞Hu-7移植瘤裸鼠模型的影响
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摘要
目的:探究扶正抑瘤汤对人肝癌细胞Hu-7移植瘤裸鼠模型的影响。方法:对75只裸鼠进行人肝癌细胞Hu-7移植建模后,随机分成对照组、5-氟尿嘧啶组、高剂量扶正抑瘤汤组、中剂量扶正抑瘤汤组、低剂量扶正抑瘤汤组,分别给予生理盐水、5-氟尿嘧啶、扶正抑瘤汤(高、中、低剂量)干预3周后处死,记录和比较5组裸鼠的移植瘤质量、抑瘤率、凋亡细胞数量、自噬小体数量和Beclin-1、LC3、Bnip3表达情况。结果:5-氟尿嘧啶组、高剂量扶正抑瘤汤组、中剂量扶正抑瘤汤组、低剂量扶正抑瘤汤组裸鼠的肝癌移植瘤平均质量均低于对照组、抑瘤率均高于对照组(P<0.05);与中剂量扶正抑瘤汤组和低剂量扶正抑瘤汤组比较,5-氟尿嘧啶组、高剂量扶正抑瘤汤组裸鼠的肝癌移植瘤平均质量均降低,抑瘤率增加(P<0.05);电镜下,5-氟尿嘧啶组、高剂量扶正抑瘤汤组裸鼠的移植瘤细胞双层膜自噬小体数量较多,而中剂量扶正抑瘤汤组、低剂量扶正抑瘤汤组裸鼠的移植瘤细胞自噬小体较少;与对照组比较,5-氟尿嘧啶组、高剂量扶正抑瘤汤组、中剂量扶正抑瘤汤组的裸鼠的Beclin-1、LC3、Bnip3的表达增加(P<0.05),与中剂量扶正抑瘤汤组和低剂量扶正抑瘤汤组比较,5-氟尿嘧啶组、高剂量扶正抑瘤汤组的裸鼠的Beclin-1、LC3、Bnip3的表达增加(P<0.05)。结论:扶正抑瘤汤能抑制人肝癌细胞Hu-7移植瘤裸鼠肿瘤的生长,增强肿瘤细胞的自噬活性,以高剂量扶正抑瘤汤的作用最明显。
Objective:To investigate the effect of Fuzheng Yiliu Decoction(扶正抑瘤汤) on nude mice models of human hepatoma cell Hu-7 xenograft.Methods:Human hepatoma cell line Hu-7 was transplanted into 75 nude mice,which were randomly divided into control group,5-fluorouracil group and high dose group,medium dose group,low dose group,were given saline,5-fluorouracil,Fuzheng anti tumor decoction(high,medium and low dose) after 3 weeks of treatment were recorded and compared the transplanted tumor weight,inhibition rate,the number of apoptotic cells,the number of autophagosomes,the expression of Beclin-1,LC3 and Bnip3 between five groups.Results:Compared with the control group,the average weight of transplanted liver tumor of 5-fluorouracil group and high dose group,medium dose group,low dose group of nude mice were decreased and the inhibition rate increased(P<0.05);compared with medium dose group,Low dose group,the average weight of transplanted liver tumor 5-fluorouracil group and High dose Decoction group were decreased and the inhibition rate increased(P<0.05);under electron microscope,in the 5-fluorouracil group and the High dose group,the number of autophagic cells in the bilayer membrane of the transplanted tumor cells were higher;compared with the Control group,expression of Beclin-1,LC3,Bnip3 of 5-fluorouracil group and High dose group,Medium dose group were increased(P<0.05),compared with Medium dose group and Low dose group,expression of Beclin-1,LC3,Bnip3 of 5-fluorouracil group and High dose group were increased(P <0.05).Conclusion:Fuzheng Yiliu decoction can inhibit the growth of tumor cells in nude mice bearing human hepatoma cell Hu-7 and enhance the autophagic activity of tumor cells,and the effect of high dose Fuzheng anti tumor Decoction is most obvious.
Objective:To investigate the effect of Fuzheng Yiliu Decoction(扶正抑瘤汤) on nude mice models of human hepatoma cell Hu-7 xenograft.Methods:Human hepatoma cell line Hu-7 was transplanted into 75 nude mice,which were randomly divided into control group,5-fluorouracil group and high dose group,medium dose group,low dose group,were given saline,5-fluorouracil,Fuzheng anti tumor decoction(high,medium and low dose) after 3 weeks of treatment were recorded and compared the transplanted tumor weight,inhibition rate,the number of apoptotic cells,the number of autophagosomes,the expression of Beclin-1,LC3 and Bnip3 between five groups.Results:Compared with the control group,the average weight of transplanted liver tumor of 5-fluorouracil group and high dose group,medium dose group,low dose group of nude mice were decreased and the inhibition rate increased(P<0.05);compared with medium dose group,Low dose group,the average weight of transplanted liver tumor 5-fluorouracil group and High dose Decoction group were decreased and the inhibition rate increased(P<0.05);under electron microscope,in the 5-fluorouracil group and the High dose group,the number of autophagic cells in the bilayer membrane of the transplanted tumor cells were higher;compared with the Control group,expression of Beclin-1,LC3,Bnip3 of 5-fluorouracil group and High dose group,Medium dose group were increased(P<0.05),compared with Medium dose group and Low dose group,expression of Beclin-1,LC3,Bnip3 of 5-fluorouracil group and High dose group were increased(P <0.05).Conclusion:Fuzheng Yiliu decoction can inhibit the growth of tumor cells in nude mice bearing human hepatoma cell Hu-7 and enhance the autophagic activity of tumor cells,and the effect of high dose Fuzheng anti tumor Decoction is most obvious.
引文
[1]张武德,程卫东,鲍英存,等.扶正抑瘤汤对肝癌Hu-7细胞诱导凋亡及自噬的作用[J].中国老年学杂志,2015,21(1):6036-6039.
[2]Liu F. Experimental research of Fuzheng xiaoliu decoction on inhibiting proliferation and inducting apoptosis of human hepatoma SMMC-7721 cells[J]. Modern Practical Medicine,2012,6(8):123-127.
[3]Zhihui X U, Zhao J, You H, et al. The effects of TRAIL combining Silibinin on apoptosis of human hepatoma carcinoma cell line Huh7[J]. J IMMUNOL METHODS,2014,30(6):562-565.
[4]曾龙,马占军,吴建军,等.扶正抑瘤汤含药血清对胶质瘤环境下骨髓间充质干细胞增殖、周期和凋亡的影响[J].兰州大学学报(医学版),2016,42(5):7-13.
[5]沈爱云,严小萍,顾凌云,等.扶正抑瘤汤对结直肠癌化疗后患者机体免疫影响的研究[J].现代中西医结合杂志,2015,3(27):2980-2982.
[6]Iizuka A,Yoshie F,Amagaya S,et al. Effect of DaiSaiko-To(Da-Chai-Hu-Tang)on LDL-Receptor Gene Expression in Human Hepatoma Cell Line(HepG2)[J]. American Journal of Plant Sciences,2013,4(2):454-459.
[7]Sainz B, Barretto N, Yu X, et al. Permissiveness of human hepatoma cell lines for HCV infection[J]. J VIROL,2012,9(1):30-34.
[8]黄军,孔祥应,汪琳,等.扶正抑瘤汤联合恩度治疗晚期非小细胞肺癌的疗效观察[J].现代中西医结合杂志,2015,6(9):955-956,957.
[9]Boo L,Ho W Y,Ali N M,et al. MiRNA Transcriptome Profiling of Spheroid-Enriched Cells with Cancer Stem Cell Properties in Human Breast MCF-7 Cell Line[J]. INT J BIOL SCI,2016,12(4):427-445.
[10]王伟,杨海淦,罗立杰,等.加味扶正抑瘤汤含药血清对结肠癌HT-29细胞的抑制效应及其机制研究[J].广州中医药大学学报,2014,31(6):979-982,1001.
[11]Pelkonen O, Xu Q, Fan T P. Why is Research on Herbal Medicinal Products Important and How Can We Improve Its Quality?[J]. AFR J TRADIT COMPLEM,2014,4(1):1-7.
[12]PavlíkováN,BartoňováI,Balu?íkováK,et al. Differentially expressed proteins in human MCF-7 breast cancer cells sensitive and resistant to paclitaxel[J]. EXP CELL RES,2015,333(1):1-10.
[13]Yin S Y,Wei W C,Jian F Y,et al. Therapeutic applications of herbal medicines for cancer patients.[J].EVID-BASED COMPL AL,2013,2013(3):3024-3026.
[14]任凯,崇殿龙,马琳艳,等.Smac类似物LCL161对肝癌细胞增殖及凋亡的影响[J].中南大学学报(医学版),2016,41(9):898-904.
[15]刘流,郭侃,刘云,等.结合斑蝥素对人肝癌HepG2细胞增殖和凋亡的作用[J].中成药,2016,38(8):1683-1688.
[16]Wu G S,Guo J J,Bao J L,et al. Anti-cancer properties of triterpenoids isolated from Ganoderma lucidum-a review[J]. EXPERT OPIN INV DRUG,2013,22(8):981-992.
[2]Liu F. Experimental research of Fuzheng xiaoliu decoction on inhibiting proliferation and inducting apoptosis of human hepatoma SMMC-7721 cells[J]. Modern Practical Medicine,2012,6(8):123-127.
[3]Zhihui X U, Zhao J, You H, et al. The effects of TRAIL combining Silibinin on apoptosis of human hepatoma carcinoma cell line Huh7[J]. J IMMUNOL METHODS,2014,30(6):562-565.
[4]曾龙,马占军,吴建军,等.扶正抑瘤汤含药血清对胶质瘤环境下骨髓间充质干细胞增殖、周期和凋亡的影响[J].兰州大学学报(医学版),2016,42(5):7-13.
[5]沈爱云,严小萍,顾凌云,等.扶正抑瘤汤对结直肠癌化疗后患者机体免疫影响的研究[J].现代中西医结合杂志,2015,3(27):2980-2982.
[6]Iizuka A,Yoshie F,Amagaya S,et al. Effect of DaiSaiko-To(Da-Chai-Hu-Tang)on LDL-Receptor Gene Expression in Human Hepatoma Cell Line(HepG2)[J]. American Journal of Plant Sciences,2013,4(2):454-459.
[7]Sainz B, Barretto N, Yu X, et al. Permissiveness of human hepatoma cell lines for HCV infection[J]. J VIROL,2012,9(1):30-34.
[8]黄军,孔祥应,汪琳,等.扶正抑瘤汤联合恩度治疗晚期非小细胞肺癌的疗效观察[J].现代中西医结合杂志,2015,6(9):955-956,957.
[9]Boo L,Ho W Y,Ali N M,et al. MiRNA Transcriptome Profiling of Spheroid-Enriched Cells with Cancer Stem Cell Properties in Human Breast MCF-7 Cell Line[J]. INT J BIOL SCI,2016,12(4):427-445.
[10]王伟,杨海淦,罗立杰,等.加味扶正抑瘤汤含药血清对结肠癌HT-29细胞的抑制效应及其机制研究[J].广州中医药大学学报,2014,31(6):979-982,1001.
[11]Pelkonen O, Xu Q, Fan T P. Why is Research on Herbal Medicinal Products Important and How Can We Improve Its Quality?[J]. AFR J TRADIT COMPLEM,2014,4(1):1-7.
[12]PavlíkováN,BartoňováI,Balu?íkováK,et al. Differentially expressed proteins in human MCF-7 breast cancer cells sensitive and resistant to paclitaxel[J]. EXP CELL RES,2015,333(1):1-10.
[13]Yin S Y,Wei W C,Jian F Y,et al. Therapeutic applications of herbal medicines for cancer patients.[J].EVID-BASED COMPL AL,2013,2013(3):3024-3026.
[14]任凯,崇殿龙,马琳艳,等.Smac类似物LCL161对肝癌细胞增殖及凋亡的影响[J].中南大学学报(医学版),2016,41(9):898-904.
[15]刘流,郭侃,刘云,等.结合斑蝥素对人肝癌HepG2细胞增殖和凋亡的作用[J].中成药,2016,38(8):1683-1688.
[16]Wu G S,Guo J J,Bao J L,et al. Anti-cancer properties of triterpenoids isolated from Ganoderma lucidum-a review[J]. EXPERT OPIN INV DRUG,2013,22(8):981-992.