槲皮素对人骨肉瘤MG-63细胞周期及自噬流的影响
|
摘要
【目的】观察槲皮素对人骨肉瘤MG-63细胞体外生长周期及自噬的影响。【方法】以不同质量浓度的槲皮素作用于体外培养的MG-63细胞,流式细胞术检测MG-63细胞生长周期,荧光显微镜观察单体红色荧光蛋白(mRFP)—绿色荧光蛋白(GFP)—微管相关蛋白轻链3(LC3)双标腺病毒转染MG-63细胞后自噬小体的形成和自噬流的变化。【结果】槲皮素显著增加处于G1期的骨肉瘤MG-63细胞数量,上调MG-63细胞自噬水平。【结论】槲皮素可抑制人骨肉瘤MG-63细胞的增殖,使其停滞在细胞周期G1期,并诱导细胞自噬。
Objective To investigate the effect of quercetin on in-vitro cell cycle and autophagy of human osteosarcoma MG-63 cells. Methods Human osteosarcoma MG-63 cells were cultured by different mass concentrations of quercetin in vitro. The cell cycle of MG-63 cells was measured by flow cytometry(FCM),and the63 cells transfected by monomeric red fluorescent protein(mRFP)-green fluorescent protein(GFP)-microtubule-associated protein 1 light chain 3(LC3)adenovirus were observed under fluorescence microscope. Results Quercetin significantly increased the proportion of MG-63 cells in G1 phase,and raised the level of autophagy of MG-63 cells. Conclusion Quercetin can inhibit the proliferation of human osteosarcoma MG-63 cells,arrest the cells restrain in G1 phase,and induce autophagy.
Objective To investigate the effect of quercetin on in-vitro cell cycle and autophagy of human osteosarcoma MG-63 cells. Methods Human osteosarcoma MG-63 cells were cultured by different mass concentrations of quercetin in vitro. The cell cycle of MG-63 cells was measured by flow cytometry(FCM),and the63 cells transfected by monomeric red fluorescent protein(mRFP)-green fluorescent protein(GFP)-microtubule-associated protein 1 light chain 3(LC3)adenovirus were observed under fluorescence microscope. Results Quercetin significantly increased the proportion of MG-63 cells in G1 phase,and raised the level of autophagy of MG-63 cells. Conclusion Quercetin can inhibit the proliferation of human osteosarcoma MG-63 cells,arrest the cells restrain in G1 phase,and induce autophagy.
引文
[1]Mirabello L,Troisi R J,Savage S A.Osteosarcoma incidence and survival rates from 1973 to 2004:data from the Surveillance,Epidemiology,and End Results Program[J].Cancer,2009,115(7):1531.
[2]Aragon-Ching J B,Maki R G.Treatment of adult soft tissue sarcoma:old concepts,new insights,and potential for drug discovery[J].Cancer Invest,2012,30(4):300.
[3]Chang J,Li Y,Wang X,et al.Polyphyllin I suppresses human osteosarcoma growth by inactivation of Wnt/beta-catenin pathway in vitro and in vivo[J].Sci Rep,2017,7(1):7605.
[4]叶蕻芝,李会婷,李西海,等.槲皮素对人软骨肉瘤细胞活性的抑制作用[J].福建中医药大学学报,2012,22(1):22.
[5]Yang F,Song L,Wang H,et al.Quercetin in prostate cancer:chemotherapeutic and chemopreventive effects,mechanisms and clinical application potential(Review)[J].Oncol Rep,2015,33(6):2659.
[6]Zhang J Y,Lin M T,Zhou M J,et al.Combinational treatment of curcumin and quercetin against gastric cancer MGC-803 cells in vitro[J].Molecules(Basel,Switzerland),2015,20(6):11524.
[7]Lan H,Hong W,Fan P,et al.Quercetin inhibits cell migration and invasion in human osteosarcoma cells[J].Cell Physiol Biochem,2017,43(2):553.
[8]孙南阳,符芳姿,谭江波.槲皮素对人成骨肉瘤MG-63细胞体外增殖与凋亡的影响[J].湖南中医杂志,2018,9(34):158.
[9]黄桢钧,刘彬,刘本荣,等.双荧光mRFP-eGFP-LC3体系在细胞自噬中的作用[J].贵州医学院学报,2015,40(10):1029.
[10]耿威,仉慧颖,李林,等.槲皮素对胃癌细胞增殖及侵袭的调控作用及机制[J].中国老年学杂志,2018,38(1):34.
[11]张杰,吕蔡,张平安,等.槲皮素抑制人宫颈癌HeLa细胞增殖的实验研究[J].中国肿瘤临床,2006,33(8):436.
[12]江波,李灿,林清,等.槲皮素体内外抑制人骨肉瘤细胞株143 B增殖及其机制研究[J].实用药物与临床,2015,18(5):517.
[13]Wang Q,Chen Z,Diao X,et al.Induction of autophagydependent apoptosis by the survivin suppressant YM155 in prostate cancer cells[J].Cancer lett,2011,302(1):29.
[14]鞠杰,刘焕彩,王巧真,等.自噬与骨肉瘤研究进展[J].中国老年学杂志,2016,36(23):6024.
[15]Liu Y,Gong W,Yang Z Y,et al.Quercetin induces protective autophagy and apoptosis through ER stress via the p-STAT3/Bcl-2axis in ovarian cancer[J].Apoptosis,2017,22(4):544.
[16]Granato M,Rizzello C,Gilardini Montani M S,et al.Quercetin induces apoptosis and autophagy in primary effusion lymphoma cells by inhibiting PI3K/AKT/mTOR and STAT3 signaling pathways[J].J Nutr Biochem,2017,41:124.
[17]方坤,刘金成,呼晓,等.槲皮素诱导人食管癌Eca-109细胞发生自噬及其作用的研究[J].哈尔滨医科大学学报,2016,50(6):484.
[2]Aragon-Ching J B,Maki R G.Treatment of adult soft tissue sarcoma:old concepts,new insights,and potential for drug discovery[J].Cancer Invest,2012,30(4):300.
[3]Chang J,Li Y,Wang X,et al.Polyphyllin I suppresses human osteosarcoma growth by inactivation of Wnt/beta-catenin pathway in vitro and in vivo[J].Sci Rep,2017,7(1):7605.
[4]叶蕻芝,李会婷,李西海,等.槲皮素对人软骨肉瘤细胞活性的抑制作用[J].福建中医药大学学报,2012,22(1):22.
[5]Yang F,Song L,Wang H,et al.Quercetin in prostate cancer:chemotherapeutic and chemopreventive effects,mechanisms and clinical application potential(Review)[J].Oncol Rep,2015,33(6):2659.
[6]Zhang J Y,Lin M T,Zhou M J,et al.Combinational treatment of curcumin and quercetin against gastric cancer MGC-803 cells in vitro[J].Molecules(Basel,Switzerland),2015,20(6):11524.
[7]Lan H,Hong W,Fan P,et al.Quercetin inhibits cell migration and invasion in human osteosarcoma cells[J].Cell Physiol Biochem,2017,43(2):553.
[8]孙南阳,符芳姿,谭江波.槲皮素对人成骨肉瘤MG-63细胞体外增殖与凋亡的影响[J].湖南中医杂志,2018,9(34):158.
[9]黄桢钧,刘彬,刘本荣,等.双荧光mRFP-eGFP-LC3体系在细胞自噬中的作用[J].贵州医学院学报,2015,40(10):1029.
[10]耿威,仉慧颖,李林,等.槲皮素对胃癌细胞增殖及侵袭的调控作用及机制[J].中国老年学杂志,2018,38(1):34.
[11]张杰,吕蔡,张平安,等.槲皮素抑制人宫颈癌HeLa细胞增殖的实验研究[J].中国肿瘤临床,2006,33(8):436.
[12]江波,李灿,林清,等.槲皮素体内外抑制人骨肉瘤细胞株143 B增殖及其机制研究[J].实用药物与临床,2015,18(5):517.
[13]Wang Q,Chen Z,Diao X,et al.Induction of autophagydependent apoptosis by the survivin suppressant YM155 in prostate cancer cells[J].Cancer lett,2011,302(1):29.
[14]鞠杰,刘焕彩,王巧真,等.自噬与骨肉瘤研究进展[J].中国老年学杂志,2016,36(23):6024.
[15]Liu Y,Gong W,Yang Z Y,et al.Quercetin induces protective autophagy and apoptosis through ER stress via the p-STAT3/Bcl-2axis in ovarian cancer[J].Apoptosis,2017,22(4):544.
[16]Granato M,Rizzello C,Gilardini Montani M S,et al.Quercetin induces apoptosis and autophagy in primary effusion lymphoma cells by inhibiting PI3K/AKT/mTOR and STAT3 signaling pathways[J].J Nutr Biochem,2017,41:124.
[17]方坤,刘金成,呼晓,等.槲皮素诱导人食管癌Eca-109细胞发生自噬及其作用的研究[J].哈尔滨医科大学学报,2016,50(6):484.