25种中草药对3种鱼源嗜水气单胞菌的抑杀效果比较
|
摘要
为了研究25种中草药对鲤鱼、青鱼和草鱼源嗜水气单胞菌(Aeromonas hydrophila)的体外抑菌效果,并筛选出具有较好抑菌作用的单味中草药,试验采用水提法提取中草药的有效成分,通过牛津杯打孔法和微量二倍稀释法分别测定了25种中草药对3种不同来源嗜水气单胞菌的抑菌圈直径、最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果表明:黄柏、丁香、艾叶等9种中草药对鲤鱼源嗜水气单胞菌有抑菌作用,抑菌圈直径为10~22 mm,MIC为3. 2~12. 5 mg/mL,MBC为6. 3~25 mg/mL;赤芍、大青叶、茵陈等12种中草药对青鱼源嗜水气单胞菌有抑菌效果,抑菌圈直径为10~22 mm,MIC为3. 2~12. 5 mg/mL,MBC为6. 3~25 mg/mL;赤芍、丁香、黄柏等12种中草药对草鱼源嗜水气单胞菌有抑菌效果,抑菌圈直径为12~28 mm,MIC为3. 2~12. 5 mg/mL,MBC为6. 3~25 mg/mL。说明不同来源的嗜水气单胞菌对不同中草药的敏感性不同。
The aim of the present study was to investigate the bacteriostatic effect of 25 Chinese herbal medicines on Aeromonas hydrophila isolated from carp,black carp and grass carp,and to screen out the single Chinese herbal medicine with good bacteriostasis. The effective components of Chinese herbal medicine were extracted by water extraction method. The diameters of inhibition zone,minimum inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) of 25 kinds of Chinese herbal medicines against 3 kinds of Aeromonas hydrophila from different sources were determined by Oxford cup punch and trace double dilution method. The results showed that the 9 kinds of Chinese herbal medicine,such as cortex phellodendri,clove,argyi and so on,had bacteriostatic effect on Aeromonas hydrophila isolated from carp. The diameters of inhibition zone were 10-22 mm,MIC were 3. 2-12. 5 mg/mL,MBC were 6. 3-25 mg/mL,respectively. The 12 kinds of Chinese herbal medicine,such as red paeonia,daqing,Chen and so on,had bacteriostatic effect on Aeromonas hydrophila from black carp. The diameter of bacteriostatic zone was 10-22 mm,MIC was 3. 2-12. 5 mg/mL,MBC was 6. 3-25 mg/mL. The 12 kinds of Chinese herbal medicine,such as red paeonia,cloves,yellow cypress and so on,had bacteriostatic effect on Aeromonas hydrophila from grass carp. The diameter of inhibition zone was 12-28 mm,MIC was 3. 2-12. 5 mg/mL,MBC was 6. 3-25 mg/mL. The results indicated that the sensitivity of Aeromonas hydrophila from different sources to different Chinese herbal medicines was different.
The aim of the present study was to investigate the bacteriostatic effect of 25 Chinese herbal medicines on Aeromonas hydrophila isolated from carp,black carp and grass carp,and to screen out the single Chinese herbal medicine with good bacteriostasis. The effective components of Chinese herbal medicine were extracted by water extraction method. The diameters of inhibition zone,minimum inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) of 25 kinds of Chinese herbal medicines against 3 kinds of Aeromonas hydrophila from different sources were determined by Oxford cup punch and trace double dilution method. The results showed that the 9 kinds of Chinese herbal medicine,such as cortex phellodendri,clove,argyi and so on,had bacteriostatic effect on Aeromonas hydrophila isolated from carp. The diameters of inhibition zone were 10-22 mm,MIC were 3. 2-12. 5 mg/mL,MBC were 6. 3-25 mg/mL,respectively. The 12 kinds of Chinese herbal medicine,such as red paeonia,daqing,Chen and so on,had bacteriostatic effect on Aeromonas hydrophila from black carp. The diameter of bacteriostatic zone was 10-22 mm,MIC was 3. 2-12. 5 mg/mL,MBC was 6. 3-25 mg/mL. The 12 kinds of Chinese herbal medicine,such as red paeonia,cloves,yellow cypress and so on,had bacteriostatic effect on Aeromonas hydrophila from grass carp. The diameter of inhibition zone was 12-28 mm,MIC was 3. 2-12. 5 mg/mL,MBC was 6. 3-25 mg/mL. The results indicated that the sensitivity of Aeromonas hydrophila from different sources to different Chinese herbal medicines was different.
引文
[1]杨斌.虹鳟烂鳃病病原调查报告[J].水产科技情报,2005,32(6):273-274,277.
[2]张翠娟,于宙亮,赵宝华,等.嗜水气单胞菌研究进展[J].中国兽药杂志,2008,42(7):46-50.
[3]吕爱军,胡秀彩,李槿年,等.水产动物致病性嗜水气单胞菌的分离鉴定[J].江苏农业科学,2008(2):172-174.
[4]靳晓敏,葛慕湘,张艳英,等.不同来源嗜水气单胞菌耐药基因的检测[J]. 2015,45(3):84-87.
[5]王远微,汤承,于学辉,等.三重PCR检测鱼类致病性嗜水气单胞菌[J].微生物学报,2008,48(7):947-951.
[6]VIVEKANANDHAN G,SAVITHAMANI K,HATHA A,et al. Antibiotic resistance of Aeromonas hydrophila isolated form marketed fish and prawn of South India[J]. Int J Food Microbiol,2002,76(1/2):165-168.
[7]道金,郑开强,黄光伟.烤烟生长后期喷施叶面肥对上部叶质量的影响[J].福建农业科技,2006(1):48-50.
[8]钱存柔,黄仪秀.微生物学实验教程[M].北京:北京大学出版社,2005.
[9]王洪彬,李永慧,吴同垒,等.中草药对美人鱼发光杆菌的体外抑菌试验[J].黑龙江畜牧兽医,2018(06):155-157.
[10]何爱华,张曦,陶琳丽,等. 6种中草药对4种淡水鱼致病菌体外抑菌作用的研究[J].南方水产科学,2011,2(7):73-75.
[11]高汉娇,林永泰,陈昌福,等. 21种中草药对嗜水气单胞菌的试管内抑菌作用[J].水利渔业,1996(4):16-17.
[12]曹红峰,宋靖芳,李国庆,等.中草药对嗜水气单胞菌ST-3-3抑菌作用的研究[J].中医药导报,2007,5(13):86-87.
[13]张文青,龚一富,金思,等.中草药及其配伍对嗜水气单胞菌的抑菌作用[J].渔业科学进展,2012,33(1):114-121.
[14]郭秀平,何有根,林明辉,等. 45种中草药提取物对中华鳖源嗜水气单胞菌的体外抑菌作用[J].广东农业科学,2012,39(20):125-127.
[2]张翠娟,于宙亮,赵宝华,等.嗜水气单胞菌研究进展[J].中国兽药杂志,2008,42(7):46-50.
[3]吕爱军,胡秀彩,李槿年,等.水产动物致病性嗜水气单胞菌的分离鉴定[J].江苏农业科学,2008(2):172-174.
[4]靳晓敏,葛慕湘,张艳英,等.不同来源嗜水气单胞菌耐药基因的检测[J]. 2015,45(3):84-87.
[5]王远微,汤承,于学辉,等.三重PCR检测鱼类致病性嗜水气单胞菌[J].微生物学报,2008,48(7):947-951.
[6]VIVEKANANDHAN G,SAVITHAMANI K,HATHA A,et al. Antibiotic resistance of Aeromonas hydrophila isolated form marketed fish and prawn of South India[J]. Int J Food Microbiol,2002,76(1/2):165-168.
[7]道金,郑开强,黄光伟.烤烟生长后期喷施叶面肥对上部叶质量的影响[J].福建农业科技,2006(1):48-50.
[8]钱存柔,黄仪秀.微生物学实验教程[M].北京:北京大学出版社,2005.
[9]王洪彬,李永慧,吴同垒,等.中草药对美人鱼发光杆菌的体外抑菌试验[J].黑龙江畜牧兽医,2018(06):155-157.
[10]何爱华,张曦,陶琳丽,等. 6种中草药对4种淡水鱼致病菌体外抑菌作用的研究[J].南方水产科学,2011,2(7):73-75.
[11]高汉娇,林永泰,陈昌福,等. 21种中草药对嗜水气单胞菌的试管内抑菌作用[J].水利渔业,1996(4):16-17.
[12]曹红峰,宋靖芳,李国庆,等.中草药对嗜水气单胞菌ST-3-3抑菌作用的研究[J].中医药导报,2007,5(13):86-87.
[13]张文青,龚一富,金思,等.中草药及其配伍对嗜水气单胞菌的抑菌作用[J].渔业科学进展,2012,33(1):114-121.
[14]郭秀平,何有根,林明辉,等. 45种中草药提取物对中华鳖源嗜水气单胞菌的体外抑菌作用[J].广东农业科学,2012,39(20):125-127.