摘要
影响纳豆激酶产生的因素主要有Na_2HPO_4浓度、温度、种龄、蛋白胨浓度、p H值等。研究优化了纳豆激酶产生条件,结果表明,蛋白胨、Na_2HPO_4和MgSO_4浓度分别为2. 57%、0. 28%和1. 37%时,获得最大产酶量1 429 IU/m L。
This paper mainly discusses the factors of Na_2HPO_4,MgSO_4,temperature,seed age,peptone and p H value,and optimizes the conditions for the production of nattokinase. The results showed that the maximum enzyme yield1 429 IU/m L was obtained when the mass scores of peptone,Na_2HPO_4 and MgSO_4 were 2. 57%,0. 28% and 1. 37%.
引文
[1]孔凡真.日本国民食物结构模式及其特点[J].美食景观,2007,(06):37.
[2]杨亚平.中国应重视纳豆的研制和推广[J].中国食物与营养,2001,(01):25-27.
[3] Urano T. Thara H. Umemura K. et al. The profibrinolytic enzyme subtilisin NAT puri-fied from Bacillus subtilis cleaves and inactivator inhibitor type 1[J]. J. Biol. Chen.,2001,276(27):24690-24696.
[4]代增英,冯建岭,李迎秋.纳豆及纳豆激酶的研究进展[J].山东食品发酵2013,(02):43-50.
[5]陈志文,徐尔尼,肖美燕.纳豆激酶的研究进展[J].食品科学,2002,23(10):130-134.
[6] SUMI H,HAMADA H. TSUSHIMA H. et al. A novel fibrinolyticenzyme(nattokinase)in the vegetable cheese Natto:a typical and popular soybean food in the Japanese diet[J]. Experientia,1987,43(10):1110-1111.
[7]高瑞萍,刘辉,刘嘉,等.纳豆的研究进展[J].食品与发酵科技,2011,47(01):23-26.
[8]范强,姚文兵,高向东,等.纳豆激酶分离纯化及其性质研究[J].中国天然药物,2005,3(02):124-127.