芫花素对大鼠离体子宫收缩作用的研究
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摘要
目的研究芫花素(益母草中的活性物质)对大鼠离体子宫收缩的影响及其作用机制。方法制作大鼠子宫肌条,观察芫花素(10~(-7)～10~(-4) mol/L)和缩宫素(10~(-7)～10~(-4) mol/L)对大鼠离体子宫平滑肌条自发收缩振幅的影响。建立大鼠离体子宫平滑肌收缩模型,分为缩宫素0.002U/ml、芫花素1.5μg/ml(小剂量)、芫花素3μg/ml(中剂量)、芫花素6μg/ml(大剂量)组,观察给药前后子宫平滑肌收缩幅度及舒张幅度变化。结果芫花素和缩宫素均有效地诱导子宫平滑肌条的收缩,且两组效果无显著性差异(P>0.05);芫花素各组对大鼠离体子宫平滑肌的收缩值和舒张值均有显著的增强作用(P<0.01),并且发现其对子宫平滑肌条的收缩作用与缩宫素相当(P>0.05)。结论芫花素对大鼠离体子宫平滑肌具有显著的收缩作用,为芫花素用于产后出血等疾病的临床应用提供药理实验依据。
Objective:The effects of genkwanin,one of constituent in Leonurus Artemisia,were investigated in the contraction of isolated rat's uterus and action of genkwanin was explored in this study.Methods:The uterine smooth muscle strips were made to observe the effect of genkwanin(10~(-7)～10~(-4) mol/L)and oxytocin(10~(-7)～10~(-4) mol/L)on the spontaneous contraction amplitude.Utilizing the rat isolated uterine smooth muscle contraction model successfully developed,we divided groups into oxytocin 0.002 U/ml,genkwanin 1.5μg/ml(small dose),and genkwanin 3μg/ml(medium dose)and genkwanin 6μg/ml(large dose),observing before and after the uterine smooth muscle contraction amplitude and uterine diastolic amplitude changes.Results:Genkwanin and oxytocin both effectively induced the contraction of myometrium of the uterus and had no significant difference(P>0.0 5).All genkwanin groups had a certain enhancement effect on the contraction and diastolic values of the isolated uterine smooth muscle of the rats,and there was a significant difference before and after the administration(P<0.0 1).It was found that the effect of genkwanin on the contraction of uterine smooth muscle strips was similar to that of oxytocin(P>0.0 5).Conclusions:Genkwanin has obvious contractile effect on the isolated uterine smooth muscle of rats.Our research provides pharmacological experimental basis of genkwanin for the clinical application like postpartum hemorrhage.
Objective:The effects of genkwanin,one of constituent in Leonurus Artemisia,were investigated in the contraction of isolated rat's uterus and action of genkwanin was explored in this study.Methods:The uterine smooth muscle strips were made to observe the effect of genkwanin(10~(-7)～10~(-4) mol/L)and oxytocin(10~(-7)～10~(-4) mol/L)on the spontaneous contraction amplitude.Utilizing the rat isolated uterine smooth muscle contraction model successfully developed,we divided groups into oxytocin 0.002 U/ml,genkwanin 1.5μg/ml(small dose),and genkwanin 3μg/ml(medium dose)and genkwanin 6μg/ml(large dose),observing before and after the uterine smooth muscle contraction amplitude and uterine diastolic amplitude changes.Results:Genkwanin and oxytocin both effectively induced the contraction of myometrium of the uterus and had no significant difference(P>0.0 5).All genkwanin groups had a certain enhancement effect on the contraction and diastolic values of the isolated uterine smooth muscle of the rats,and there was a significant difference before and after the administration(P<0.0 1).It was found that the effect of genkwanin on the contraction of uterine smooth muscle strips was similar to that of oxytocin(P>0.0 5).Conclusions:Genkwanin has obvious contractile effect on the isolated uterine smooth muscle of rats.Our research provides pharmacological experimental basis of genkwanin for the clinical application like postpartum hemorrhage.
引文
[1]阮金兰,杜俊蓉,曾庆忠,等.益母草的化学、药理和临床研究进展[J].中草药,2003,34:112-116.
[2]Fan J,Wei F,Zhang Y,et al.Combining Sprague-Dawley rat uterus cell membrane chromatography with HPLC/MS to screen active components from Leonurus artemisia[J].Pharm Biol,2016,54:279-284.
[3]Han SL,Zhang P,Wei F,et al.Screening active components acting onα1A adrenergic receptors from agrimony using a Sprague-Dawley rat prostate cell membrane chromatography online coupled HPLC/MS method[J].Anal Methods,2012,4:3351-3357.
[4]徐爱群,曾蔚越,吴大蓉,等.益母草注射液缩宫止血疗效初步观察[J].中华妇幼临床医学杂志,2007,3:88-90.
[5]林建华,林其德,刘兴会,等.阴道分娩中益母草注射液预防产后出血促进子宫收缩的多中心临床研究[J].实用妇产科杂志,2009,25:44-47.
[6]金若敏,陈兆善,陈长勋,等.益母草治疗痛经机制探索[J].中国现代应用药学,2004,21:90-93.
[7]刘圣君,任君旭,刘艳凯,等.益母草对失血性休克大鼠血流动力学的影响[J].中国临床康复,2006,10:70-72.
[8]申利红,王胜利.益母草的研究进展[J].安徽农业科学,2010,38:4414-4416.
[9]陈少如,郑鸿翱,陈韩秋,等.益母草制剂治疗心肌缺血及其机制研究[J].中国危重病急救医学,2002,14:19-22.
[10]郭鹏,高颖,张静泽,等.益母草的药理学研究进展[J].武警医学院学报(医学版),2008,17:83-84.
[11]Woiski MD,van Vugt HC,Dijkman A,et al.From postpartum haemorrhage guideline to local protocol:a study of protocol quality[J].Matern Child Health J,2016,20:2160-2168.
[12]Haver J,Ansari N,Zainullah P,et al.Misoprostol for prevention of postpartum hemorrhage at home birth in Afghanistan:program expansion experience[J].J Midwifery Womens Health,2016,61:196-202.
[13]Lin YR,Wu MY,Chiang JH,et al.The utilization of traditional Chinese medicine in patients with dysfunctional uterine bleeding in Taiwan:a nationwide population-based study[J].BMC Complement Altern Med,2017,17:427.
[14]Weeks AD.Tranexamic acid for postpartum haemorrhage:a major advance[J].Lancet Glob Health,2018,6:e132-e133.
[15]Liu J,Peng C,Zhou QM,et al.Alkaloids and flavonoid glycosides from the aerial parts of Leonurus japonicus and their opposite effects on uterine smooth muscle[J].Photochemistry,2018,145:128-136.
[16]Liu W,Ma S,Pan W,et al.Combination of motherwort injection and oxytocin for the prevention of postpartum hemorrhage after cesarean section[J].J Matern Fetal Neonatal Med,2016,29:2490-2493.
[17]郭起岳,吴清,操红缨,等.乌鸡白凤丸治疗原发性痛经的实验研究[J].西北药学杂志,2016,31:482-485.
[18]de Boer HJ,Cotingting C.Medicinal plants for women’s healthcare in southeast Asia:a meta-analysis of their traditional use,chemical constituents,and pharmacology[J].JEthnopharmacol,2014,151:747-767.
[19]Shang X,Pan H,Wang X,et al.Leonurus japonicus Houtt.:ethnopharmacology,phytochemistry and pharmacology of an important traditional Chinese medicine[J].J Ethnopharmacol,2014,152:14-32.
[20]Pang H,Wang J,Tang Y,et al.Comparative analysis of the main bioactive components of Xin-Sheng-Hua granule and its single herbs by ultrahigh performance liquid chromatography with tandem mass spectrometry[J].J Sep Sci,2016,39:4096-4106.
[21]赵丁,刘伟娜,王伟,等.益母草提取物对小鼠体外子宫收缩功能的影响[J].医药导报,2008,27:640-642.
[22]石米扬,昌兰芳,何功倍.红花、当归、益母草对子宫兴奋作用的机理研究[J].中国中药杂志,1995,20:173-175.
[2]Fan J,Wei F,Zhang Y,et al.Combining Sprague-Dawley rat uterus cell membrane chromatography with HPLC/MS to screen active components from Leonurus artemisia[J].Pharm Biol,2016,54:279-284.
[3]Han SL,Zhang P,Wei F,et al.Screening active components acting onα1A adrenergic receptors from agrimony using a Sprague-Dawley rat prostate cell membrane chromatography online coupled HPLC/MS method[J].Anal Methods,2012,4:3351-3357.
[4]徐爱群,曾蔚越,吴大蓉,等.益母草注射液缩宫止血疗效初步观察[J].中华妇幼临床医学杂志,2007,3:88-90.
[5]林建华,林其德,刘兴会,等.阴道分娩中益母草注射液预防产后出血促进子宫收缩的多中心临床研究[J].实用妇产科杂志,2009,25:44-47.
[6]金若敏,陈兆善,陈长勋,等.益母草治疗痛经机制探索[J].中国现代应用药学,2004,21:90-93.
[7]刘圣君,任君旭,刘艳凯,等.益母草对失血性休克大鼠血流动力学的影响[J].中国临床康复,2006,10:70-72.
[8]申利红,王胜利.益母草的研究进展[J].安徽农业科学,2010,38:4414-4416.
[9]陈少如,郑鸿翱,陈韩秋,等.益母草制剂治疗心肌缺血及其机制研究[J].中国危重病急救医学,2002,14:19-22.
[10]郭鹏,高颖,张静泽,等.益母草的药理学研究进展[J].武警医学院学报(医学版),2008,17:83-84.
[11]Woiski MD,van Vugt HC,Dijkman A,et al.From postpartum haemorrhage guideline to local protocol:a study of protocol quality[J].Matern Child Health J,2016,20:2160-2168.
[12]Haver J,Ansari N,Zainullah P,et al.Misoprostol for prevention of postpartum hemorrhage at home birth in Afghanistan:program expansion experience[J].J Midwifery Womens Health,2016,61:196-202.
[13]Lin YR,Wu MY,Chiang JH,et al.The utilization of traditional Chinese medicine in patients with dysfunctional uterine bleeding in Taiwan:a nationwide population-based study[J].BMC Complement Altern Med,2017,17:427.
[14]Weeks AD.Tranexamic acid for postpartum haemorrhage:a major advance[J].Lancet Glob Health,2018,6:e132-e133.
[15]Liu J,Peng C,Zhou QM,et al.Alkaloids and flavonoid glycosides from the aerial parts of Leonurus japonicus and their opposite effects on uterine smooth muscle[J].Photochemistry,2018,145:128-136.
[16]Liu W,Ma S,Pan W,et al.Combination of motherwort injection and oxytocin for the prevention of postpartum hemorrhage after cesarean section[J].J Matern Fetal Neonatal Med,2016,29:2490-2493.
[17]郭起岳,吴清,操红缨,等.乌鸡白凤丸治疗原发性痛经的实验研究[J].西北药学杂志,2016,31:482-485.
[18]de Boer HJ,Cotingting C.Medicinal plants for women’s healthcare in southeast Asia:a meta-analysis of their traditional use,chemical constituents,and pharmacology[J].JEthnopharmacol,2014,151:747-767.
[19]Shang X,Pan H,Wang X,et al.Leonurus japonicus Houtt.:ethnopharmacology,phytochemistry and pharmacology of an important traditional Chinese medicine[J].J Ethnopharmacol,2014,152:14-32.
[20]Pang H,Wang J,Tang Y,et al.Comparative analysis of the main bioactive components of Xin-Sheng-Hua granule and its single herbs by ultrahigh performance liquid chromatography with tandem mass spectrometry[J].J Sep Sci,2016,39:4096-4106.
[21]赵丁,刘伟娜,王伟,等.益母草提取物对小鼠体外子宫收缩功能的影响[J].医药导报,2008,27:640-642.
[22]石米扬,昌兰芳,何功倍.红花、当归、益母草对子宫兴奋作用的机理研究[J].中国中药杂志,1995,20:173-175.