内消瘰疬片中碘含量的测定
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摘要
目的测定内消瘰疬片中有机碘含量。方法将药物灰化后,分别采用比色法、紫外-可见分光光度法和高效液相色谱法测定碘含量,最终选用HPLC法,Athena-NH_2色谱柱,0.1%磷酸二氢钾溶液-甲醇(80∶20)为流动相,流速为0.7mL·min~(-1),检测波长为220nm。结果碘色谱峰分离良好,重现性好,无干扰,测得内消瘰疬片中碘含量为91.7μg·g~(-1)。结论灰化-高效液相色谱法可用于测定药物中有机碘的含量。
OBJECTIVE To determine the organic iodine content in Neixiao tablets.METHODS The drugs were ashed,following determined the iodine content by colorimetric method,UV-VIS spectrophotometry and high-performance liquid chromatography(HPLC) method,respectively.Ultimately,the HPLC method was adopted with Athena-NH_2 column and 0.1% KH_2PO_4-MeOH(80/20,v/v) as mobile phase at 0.7 mL·min~(-1) and 220 nm.RESULTS The iodine chromatographic peak was well separated without interference,and it possessed good reproducibility.The iodine content in Neixiao Tablet was 91.7μg·g~(-1).CONCLUSION Ashing-HPLC method could served for determination of organic iodine content in drugs.
OBJECTIVE To determine the organic iodine content in Neixiao tablets.METHODS The drugs were ashed,following determined the iodine content by colorimetric method,UV-VIS spectrophotometry and high-performance liquid chromatography(HPLC) method,respectively.Ultimately,the HPLC method was adopted with Athena-NH_2 column and 0.1% KH_2PO_4-MeOH(80/20,v/v) as mobile phase at 0.7 mL·min~(-1) and 220 nm.RESULTS The iodine chromatographic peak was well separated without interference,and it possessed good reproducibility.The iodine content in Neixiao Tablet was 91.7μg·g~(-1).CONCLUSION Ashing-HPLC method could served for determination of organic iodine content in drugs.
引文
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[2]蒋云霞,李蓉,钟国清,等.有机碘的研究现状及应用[J].广东微量元素科学,2010,17(10):12-16.
[3]张勇.14种微量元素与人体健康[J].化学教与学,2010,(6):79-81.
[4]车秀琴.碘与人体健康[J].科技视界,2015,(18):241.
[5]刘湘,彭德君,鲁澄宇.碘海醇药动学研究进展与应用[J].中国医院药学杂志,2016,36(5):417-421.
[6]张伟,张丽娜,陈亚双,等.聚维酮碘的临床应用[J].黑龙江中医药,2016,45(4):62-63.
[7]蒋文平.胺碘酮抗心律失常治疗应用指南[J].中华心血管病杂志,2004,(12):14-20.
[8]相萍萍,徐书杭,刘超.食物中碘的测定方法[J].中国食物与营养,2017,23(10):34-37.
[9]谢少艾,陈虹锦,马荔,等.分光光度法测定海带中的碘[J].实验室研究与探索,2005,(7):16-17.
[10]邹霈,罗世能,刘娅灵,等.高效液相色谱法测定碘佛醇的含量[J].中国新药杂志,2004,(1):1368-1370.