鼠伤寒沙门菌人工感染鹅的病理组织学观察
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摘要
为研究鼠伤寒沙门菌感染鹅的病理组织学变化,采取肌肉注射鼠伤寒沙门菌的方法,人工感染58日龄健康鹅,对试验鹅进行病理剖检,并采取心脏、肺脏、肝脏、脾脏、肾脏、脑、胰腺和肠道病料制作病理切片,观察病理组织学变化。结果可见,受感染鹅18 h内开始出现死亡,死亡率达66.7%;主要病理变化为皮下、心肌、肝脏、脾脏、肠道、脑膜等多组织器官多处充血、出血,肝脏实质肿胀,表面可见大量大小不一的雪花状坏死灶,胆囊、脾脏肿大;病理组织学变化为心脏、肝脏、脾脏、肺脏、肠道等多组织器官的细胞变性、坏死,大量红细胞及炎性细胞浸润。研究结果表明,鼠伤寒沙门菌感染鹅,致病性强、死亡率高,感染鹅可出现全身多组织器官充血、出血与组织细胞变性、坏死的急性败血症。
In this work,we studied the histopathological changes of geese infected with Salmonella typhimurium. The artificial infection was taken in 58 days old healthy geese by injecting Salmonella typhimurium,pathological examination was perfolmed on the incidence of geese. Tissues of the heart,lung,liver,spleen,kidney,brain,pancreas and intestinal disease material were couected to make pathological sections,and are obsered under light microscope. Within 18 hours,the infected geese began to die,with a mortality rate of 66. 7%. The lesions of the subcutaneous tissue,myocardium,liver,lung,spleen,intestine,meninges and other organs were found bleeding and congestion by necropsy. The liver,gallbladder and spleen were swelled,and there were a large number of irregular,different sizes of snowflake necrosis foci in the surface of the liver. The changes of the heart,liver,spleen,lung,intestine and other tissues and organs of the cells degeneration and necrosis,and a large number of red blood cells and inflammatory cell infiltration were found by histopathologic examination. The results showed that geese infected with Salmonella typhimurium presented acute septicemia with high pathogenicity and high mortality,which was characterized by hemorrhage,congestion,degeneration and necrosis,and appeared inflammatory reaction.
In this work,we studied the histopathological changes of geese infected with Salmonella typhimurium. The artificial infection was taken in 58 days old healthy geese by injecting Salmonella typhimurium,pathological examination was perfolmed on the incidence of geese. Tissues of the heart,lung,liver,spleen,kidney,brain,pancreas and intestinal disease material were couected to make pathological sections,and are obsered under light microscope. Within 18 hours,the infected geese began to die,with a mortality rate of 66. 7%. The lesions of the subcutaneous tissue,myocardium,liver,lung,spleen,intestine,meninges and other organs were found bleeding and congestion by necropsy. The liver,gallbladder and spleen were swelled,and there were a large number of irregular,different sizes of snowflake necrosis foci in the surface of the liver. The changes of the heart,liver,spleen,lung,intestine and other tissues and organs of the cells degeneration and necrosis,and a large number of red blood cells and inflammatory cell infiltration were found by histopathologic examination. The results showed that geese infected with Salmonella typhimurium presented acute septicemia with high pathogenicity and high mortality,which was characterized by hemorrhage,congestion,degeneration and necrosis,and appeared inflammatory reaction.
引文
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[2]HJamali,B Radmehr,S Ismail.Prevalence and antimicrobial resistance of Listeria,Salmonella,and Yersinia species isolates in ducks and geese[J].Poultry science,2014,93(4):1 023-1 030.
[3]任丽,包金香,王俊江.雏鹅沙门氏菌病的诊治[J].吉林畜牧兽医,2007,28(7):34,36.
[4]施开创,李凤梅,邹联斌,等.鸡源致病性沙门氏菌的分离鉴定及血清型和药物敏感性分析[J].中国畜牧兽医,2015,42(8):2 160-2 168.
[5]Yu C.-Y.,Chu C.,Chou S.-J.,et al.Comparison of the Association of Age with the Infection of Salmonella and Salmonella enterica Serovar Typhimurium in Pekin Ducks and Roman Geese[J].Poultry Science,2008,87(8):1 544-1 549.
[6]曾静静.鸭沙门氏菌在人工感染雏鸭体内的动态研究[D]:雅安:四川农业大学,2013.
[7]成建.鹅沙门氏菌病的诊治[J].农业灾害研究,2016,6(3):14-15,55.
[8]金海林.鹅沙门菌病的诊断与防治[J].中国兽医杂志,2012,48(2):2,87-88.
[9]颜彬.肠炎沙门氏菌实验感染鸭病理学及免疫组化与免疫荧光抗原定位方法的建立和应用研究[D].雅安:四川农业大学,2008.
[10]黄攀,吕朋沙,程方俊,等.雏鹅人工感染沙门菌的病理组织学观察[J].中国兽医杂志,2016,52(2):38-40,51.
[11]N Chacko,K Krithiga,J George-A,et al.Pathological investigation of an outbreak of fowl typhoid in a duck farm in Kerala[J].Indian Journal of Veterinary Pathology,2017,41(2):2,155-157.
[12]Barbosa Fernanda-de-Oliveira,Neto Oliveiro-Caetano-de-Freitas,Batista Diego-Felipe-Alves,et al.Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken[J].Elsevier Editora Ltda.,2017,48(4):755-759.