2016―2018年某院甲、乙型流感病毒流行病学特点及检测方法比较
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摘要
目的探讨甲、乙型流感病毒的流行病学特征,比较抗原检测与核酸PCR检测的差异。方法收集2016年1月至2018年1月该院收治的110例疑似流感患者,采集其咽拭子标本,同时进行甲/乙型流感病毒抗原和核酸检测,分析其流行病学特征,研究2种检测方法流感以12月份至次年的3月份为高发季节,甲、乙型流感病例分别为26例和11例,在性别和是否合并肝脏基础疾病的发病率比较,差异无统计学意义(P>0.05)。结果抗原检测与核酸PCR检测结果一致性较差(P>0.05)。甲型流感抗原检测敏感性低于核酸PCR检测(抗原阳性CT值为24.63～39.35),乙型流感病毒抗原检测与核酸PCR检测无明显的相关性。结论 12月份至1月份为流感高发季节,甲、乙型流感发病率在性别和有无肝脏基础疾病中无差异,抗原检测的灵敏度、特异度低于PCR检测。
Objective To observe the epidemiology of influenza A/B from January 2016 to January 2018,and to compare the difference between antigen and nucleic acid PCR detection.Methods A total of 110 patients with suspected influenza from Jan.2016 to Jan.2018 were selected.Pharynx swabs were collected and the antigen and nucleic acid of influenza A/B virus were detected.And epidemiology characteristics were analyzed and the consistency of the two detection methods was statistically analyzed.Results December and March were the high onset season of influenza.There were 26 cases of influenza A and 11 cases of influenza B,and there were no significant difference between sex and the incidence of basic liver diseases(P>0.05).The consistency between antigen and nucleic acid detection was poor(P>0.05).The sensitivity of influenza A antigen detection was lower than nucleic acid PCR detection(the CT value of antigen positive was between24.63-39.35),and there was no significant correlation between antigen detection and nucleic acid PCR detection of influenza B virus.Conclusion The incidence of influenza is high in December and January,and there is no difference in the incidence of influenza A/B in sex and basic liver disease,the sensitivity and specificity of antigen detection are lower than PCR detection.
Objective To observe the epidemiology of influenza A/B from January 2016 to January 2018,and to compare the difference between antigen and nucleic acid PCR detection.Methods A total of 110 patients with suspected influenza from Jan.2016 to Jan.2018 were selected.Pharynx swabs were collected and the antigen and nucleic acid of influenza A/B virus were detected.And epidemiology characteristics were analyzed and the consistency of the two detection methods was statistically analyzed.Results December and March were the high onset season of influenza.There were 26 cases of influenza A and 11 cases of influenza B,and there were no significant difference between sex and the incidence of basic liver diseases(P>0.05).The consistency between antigen and nucleic acid detection was poor(P>0.05).The sensitivity of influenza A antigen detection was lower than nucleic acid PCR detection(the CT value of antigen positive was between24.63-39.35),and there was no significant correlation between antigen detection and nucleic acid PCR detection of influenza B virus.Conclusion The incidence of influenza is high in December and January,and there is no difference in the incidence of influenza A/B in sex and basic liver disease,the sensitivity and specificity of antigen detection are lower than PCR detection.
引文
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[2]LI L,WONG J Y,WU P,et al.Heterogeneity in estimates of the impact of influenza on population mortality:A systematic review[J].Am J Epidemiol,2018,187(2):378-388.
[3]WEE K B,LEE R T C,LIN J,et al.Discovery of influenza a virus sequence Pairs and their combinations for simultaneous heterosubtypic targeting that hedge against antiviral resistance[J].Plos Comput Biol,2016,12(1):1324-1329.
[4]VIGIL A,ESTELLES A,KAUVAR L M,et al.Native human monoclonal antibodies with potent cross-lineage neutralization of influenza B viruses[J].Antimicro A-gents Chem,2018,64(31)3211-3215.
[5]KOSIK I,INCE W L,GENTLES L E,et al.Influenza Avirus hemagglutinin glycosylation compensates for antibody escape fitness costs[J].PLoS Pathog,2018,14(1):e1006796.
[6]魏凌秀.2013至2015年杭州市甲型和乙型流感病毒流行病学特征分析[J].中华临床感染病杂志,2017,4(10):974-977.
[7]ZHENG J D,HUO X X,HUAI Y,et al.Epidemiology,seasonality and treatment of hospitalized adults and adolescents with influenza in Jingzhou,China,2010―2012[J].PLoS One,2016,11(3):692-695.
[8]RYU S W,SUH I B,RYU S M,et al.Comparison of three rapid influenza diagnostic tests with digital readout systems and one conventional rapid influenza diagnostic test[J].J Clin Labo Anal,2018,32(2):156-169.
[9]GROVES H T,MCDONALD J U,LANGAT P,et al.Mouse models of influenza infection with circulating strains to test seasonal vaccine efficacy[J].Front Immunol,2018,9(1)26-31.
[10]ZHUO Z,WANG J,CHEN W,et al.A rapid on-site assay for the detection of influenza a by capillary convective PCR[J].Mol Diagn Ther,2018,86(23)1756-1759.