小麦穗发芽基因等位变异及其区域分布研究
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摘要
为了解中国不同麦区小麦种质资源抗穗发芽基因的等位变异与分布特征,利用小麦抗穗发芽相关的Vp1B3和Dorm-1标记对7个不同麦区的446份小麦种质资源的等位变异和分布差异进行了检测。结果表明:(1)利用标记Vp1B3检测出Vp1的等位基因共有3种类型,分别为Vp1Bc(与抗穗发芽相关)、Vp1Ba(与感穗发芽相关)、Vp1Bb(与抗穗发芽相关),其频率分别为62.8%、32.9%和4.3%。(2)标记Dorm-1在供试材料中共检测出2种特异性条带,分别为468bp(DormB1a,与感穗发芽相关)和606bp(DormB1b,与抗穗发芽相关),所占比例分别为98.6%和1.4%。(3)具有DormB1b基因型的种质主要分布在黄淮冬麦区和长江中下游冬麦区,比例分别为5.1%和1.9%;具有(Vp1Bc+Vp1Bb)基因型(组合)的种质在长江中下游冬麦区和西南冬麦区分布最多,比例分别为75.0%和74.1%。(4)通过标记Vp1B3和Dorm-1的综合鉴定,共筛选出同时携带Vp1与Dorm-B1位点种质6份,分别是‘陕麦611’、‘郑农19’、‘豫49-198’、‘信阳0913’、‘咸阳大穗’与‘徐州8066’,可以作为抗穗发芽育种的参考亲本。
In order to understand the allelic variation and distribution of pre-harvest sprouting(PHS)genes in whet verieties of different regions,we genotyped 446 wheat cultivars from 7 wheat regions by the functional markers Vp1 B3 and Dorm-1 related to PHS resistance in China.The results indicated that:(1)Vp1 had three allelic variations detected by the marker Vp1 B3:Vp1 Bc(related to resistant PHS),Vp1 Bb(related to resistant PHS)and Vp1 Ba(related to susceptible PHS),and their frequencies were 62.8%,4.3% and 32.9%,respectively.(2)Dorm-B1 had two kinds of specific bands detected by the marker Dorm-1:468 bp(genotype of DormB1 a,correlated with susceptible PHS)and 606 bp(genotype of DormB1 b,correlated with resistant PHS)accounted for 98.6% and 1.4%,respectively.(3)The detection by marker Dorm-1 showed wheat germplasms with the genotype of Dorm-B were distributed in Huanghuai winter wheat region(HHWWR)and Yangtze River middle-lower reaches winter wheat region(YMWWR),with the ratios of 5.1% and 1.9%,respectively.The results of Vp1 B3 indicated that wheat germplasms with(Vp1 Bc+Vp1 Bb)genotype(combination)were mostly distributed in YMWWR and Southwestern winter wheat region(SWWWR)with the ratios of 75.0% and 74.1%,respectively.(4)Through the comprehensive identification of Vp1 B3 and Dorm-1 markers,6 accessions of Vp1 and DormB1 loci related to resistant PHS were screened out,which were‘Shanmai 611',‘Zhengnong 19',‘Yu 49-198',‘Xinyang 0913',‘XianyangDasui'and ‘Xuzhou 8066',respectively.These accessions could be used as reference parents for PHS resistance breeding.
In order to understand the allelic variation and distribution of pre-harvest sprouting(PHS)genes in whet verieties of different regions,we genotyped 446 wheat cultivars from 7 wheat regions by the functional markers Vp1 B3 and Dorm-1 related to PHS resistance in China.The results indicated that:(1)Vp1 had three allelic variations detected by the marker Vp1 B3:Vp1 Bc(related to resistant PHS),Vp1 Bb(related to resistant PHS)and Vp1 Ba(related to susceptible PHS),and their frequencies were 62.8%,4.3% and 32.9%,respectively.(2)Dorm-B1 had two kinds of specific bands detected by the marker Dorm-1:468 bp(genotype of DormB1 a,correlated with susceptible PHS)and 606 bp(genotype of DormB1 b,correlated with resistant PHS)accounted for 98.6% and 1.4%,respectively.(3)The detection by marker Dorm-1 showed wheat germplasms with the genotype of Dorm-B were distributed in Huanghuai winter wheat region(HHWWR)and Yangtze River middle-lower reaches winter wheat region(YMWWR),with the ratios of 5.1% and 1.9%,respectively.The results of Vp1 B3 indicated that wheat germplasms with(Vp1 Bc+Vp1 Bb)genotype(combination)were mostly distributed in YMWWR and Southwestern winter wheat region(SWWWR)with the ratios of 75.0% and 74.1%,respectively.(4)Through the comprehensive identification of Vp1 B3 and Dorm-1 markers,6 accessions of Vp1 and DormB1 loci related to resistant PHS were screened out,which were‘Shanmai 611',‘Zhengnong 19',‘Yu 49-198',‘Xinyang 0913',‘XianyangDasui'and ‘Xuzhou 8066',respectively.These accessions could be used as reference parents for PHS resistance breeding.
引文
[1]HUMPLLREYS D G,NOLL J.Methods for characterization of preharvest sprouting resistance in a wheat breeding program[J].Euphytiea,2002,126:61-65.
[2]BLACK M,BEWLEY J D,HALMER P.The Encyclopedia of Seeds Science,Technology and Uses[M].Oxfordshire:CABI Publishing,2006:528.
[3]BAILEY P C,MCKIBBIN R S,LENTON J R,et al.Genetic map locations for orthologous Vp1genes in wheat and rice[J].Theoretical&Applied Genetics,1999,98(2):281-284.
[4]LI C D,NI P X,FRANKI M,et al.Genes controlling seed dormancy and pre-harvest sprouting in a rice-wheat-barley comparison[J].Functional&Integrative Genomics,2004,4(2):84-93.
[5]CHEN C X,CAI S B,BAI G H.A major QTL controlling seed dormancy and pre-harvest sprouting resistance on chromosome 4Ain a Chinese wheat landrace[J].Molecular Breeding,2008,21(3):351-358.
[6]KATO K,NAKAMURA W,TABIKI T,et al.Detection of loci controlling seed dormancy on group 4chromosomes of wheat and comparative mapping with rice and barley genomes[J].Theoretical&Applied Genetics,2001,102(6-7):980-985.
[7]ROY J K,PRASAD M,VARSHNEY R K,et al.Identification of a microsatellite on chromosomes 6Band a STS on 7Dof bread wheat showing an association with preharvest sprouting tolerance[J].Theoretical&Applied Genetics,1999,99(1-2):336-340.
[8]张海萍,常成,冯继明,等.小麦籽粒休眠Vp1-B1基因的等位变异检测与分离[J].分子植物育种,2009,7(2):297-302.ZHANG H P,CHANG C,FENG J M,et al.Isolation and detection of allelic variations of Vp1-B1gene associated with seed dormancy in Chinese wheat varieties[J].Molecular Plant Breeding,2009,7(2):297-302.
[9]李婷,陈杰,陈锋,等.黄淮麦区地方小麦品种子粒颜色相关基因Tamyb10-1等位变异检测[J].植物遗传资源学报,2014,15(5):1 089-1 095.LI T,CHEN J,CHEN F,et al.Molecular identification of the Tamyb10-1gene controlling the grain color in landrace wheat cultivars from Huanghuai wheat region[J].Journal of Plant Genetic Resources,2014,15(5):1 089-1 095.
[10]LIU S,SEHGAL K,LI J,et al.Cloning and characterization of a critical regulator for preharvest sprouting in wheat[J].Genetics,2013,195(1):263-273.
[11]ZHANG Y,MIAO X,XIA X,et al.Cloning of seed dormancy genes(TaSdr)associated with tolerance to pre-harvest sprouting in common wheat and development of a functional marker[J].Theoretical&Applied Genetics,2014,127(4):855-866.
[12]张春利.小麦抗穗发芽分子标记的发掘与验证[D].北京:中国农业科学院,2008.
[13]YANG Y,ZHAO X L,XIA L Q,et al.Development and validation of a Viviparous-1,STS marker for pre-harvest sprouting tolerance in Chinese wheats.[J].Theoretical&Applied Genetics,2007,115(7):971-980.
[14]杨燕,赵献林,张勇,等.4个小麦抗穗发芽分子抗性标记有效性的验证与评价[J].作物学报,2008,34(1):17-24.YANG Y,ZHAO X L,ZHANG Y,et al.Evaluation and validation of four molecular markers associated with pre-harvest sprouting tolerance in Chinese wheats[J].Acta Agronomica Sinica,2008,34(1):17-24.
[15]刘光辉,杨随庄,黄可兵,等.四川小麦穗发芽抗性鉴定及5个分子标记的有效性检验[J].麦类作物学报,2015,35(2):167-174.LIU G H,YANG S Z,HUANG K B,et al.Identification and validation of five molecular markers for PHS tolerance in Sichuan wheat varieties(lines)[J].Journal of Triticeae Crops,2015,35(2):167-174.
[16]李式昭,郑建敏,伍玲,等.四川小麦品种籽粒硬度和穗发芽抗性相关基因的分子标记鉴定[J].西南农业学报,2018,31(4):641-645.LI S Z,ZHENG J M,WU L,et al.Identification of grain hardness and resistance to pre-harvest sprouting in Sichuan wheat cultivars with molecular markers[J].Southwest China Journal of Agricultural Sciences,2018,31(4):641-645.
[17]郭凤芝,梁伟光,樊庆琦,等.穗发芽相关基因Vp1B3不同等位变异在山东小麦中的分布与演变[J].麦类作物学报,2009,29(4):575-578.GUO F Z,LIANG W G,FAN Q Q,et al.The distribution and evolution of allelic variation of Vp1B3in Shandong wheat[J].Journal of Triticeae Crops,2009,29(4):575-578.
[18]张春利,杨芳萍,宋庆杰,等.黑龙江省小麦品种穗发芽及光周期的分子检测[J].麦类作物学报,2008,28(6):930-934.ZHANG C L,YANG F P,SONG Q J,et al.Moleculer detection of pre-harvest sprouting and photoperiod character in Heilongjiang wheat cultivars[J].Journal of Triticeae Crops,2008,28(6):930-934.
[19]王亮,穆培源,徐红军,等.新疆小麦品种穗发芽Vp-1基因等位变异的分布[J].麦类作物学报,2009,(4):571-574.WANG L,MU P Y,XU H J,et al.Allelic variation of Vp-1gene in Xinjiang wheat cultivars[J].Journal of Triticeae Crops,2009,(4):571-574.
[20]马文洁,张传量,宋晓朋,等.不同麦区小麦品种穗发芽抗性及其与穗部性状的相关性[J].麦类作物学报,2016,36(10):1 269-1 274.MA W J,ZHANG C L,SONG X P,et al.Pre-harvest sprouting resistance in wheat from different wheat regions and its correlation with ear characteristics[J].Journal of Triticeae Crops,2016,36(10):1 269-1 274.
[21]张晓,张伯桥,江伟,等.扬麦系列品种品质性状相关基因的分子检测[J].中国农业科学,2015,48(19):3 779-3 793.ZHANG X,ZHANG B Q,JIANG W,et al.Molecular detection for quality traits-related genes in Yangmai series wheat cultivars[J].Scientia Agricultura Sinica,2015,48(19):3 779-3 793.
[22]刘莉,沈迎芳,马超,等.穗发芽相关基因Vp-1等位变异在西部春小麦品种中的分布研究初探[J].西南农业学报,2014,27(6):2 278-2 281.LIU L,SHEN Y F,MA C,et al.Preliminary study on distribution of allelic variation of Vp-1in western spring wheat varieties[J].Southwest China Journal of Agricultural Sciences,2014,27(6):2 278-2 281.
[23]任立世,刘进英,杨燕.107份小麦历史品种抗穗发芽基因型的检测[J].麦类作物学报,2015,35(6):752-758.REN L S,LIU J Y,YANG Y.Determination of pre-harvest sprouting resistance genotypes with Vp1A3and Vp1B3in107Chinese historical wheat cultivars[J].Journal of Triticeae Crops,2015,35(6):752-758.
[24]MURRAY H G,THOMPSON W F.Rapid isolation of high molecular weight plant DNA[J].Nucleic Acids Research,1980,8(19):4 321-4 325.
[25]朱冬梅,李曼,李东升,等.长江中下游麦区小麦新品种穗发芽抗性及鉴定方法研究[J].核农学报,2018,32(4):795-801.ZHU D M,LI M,LI D S,et al.Evaluation and determination method of pre-harvest sprouting resistance of new wheat cultivars in middle and lower reaches of Yangtze River[J].Journal of Nuclear Agricultural Sciences,2018,32(4):795-801.
[26]曹雪连,张衡,姜昊,等.分子标记PM19-A1对1015份小麦抗穗发芽基因型的筛选及其有效性验证[J].麦类作物学报,2016,36(10):1 283-1 290.CAO X L,ZHANG H,JIANG H,et al.Detection and validation of molecular marker PMl9-A1associated with pre-harvest sprouting resistance in 1015wheat varieties[J].Journal of Triticeae Crops,2016,36(10):1 283-1 290.
[2]BLACK M,BEWLEY J D,HALMER P.The Encyclopedia of Seeds Science,Technology and Uses[M].Oxfordshire:CABI Publishing,2006:528.
[3]BAILEY P C,MCKIBBIN R S,LENTON J R,et al.Genetic map locations for orthologous Vp1genes in wheat and rice[J].Theoretical&Applied Genetics,1999,98(2):281-284.
[4]LI C D,NI P X,FRANKI M,et al.Genes controlling seed dormancy and pre-harvest sprouting in a rice-wheat-barley comparison[J].Functional&Integrative Genomics,2004,4(2):84-93.
[5]CHEN C X,CAI S B,BAI G H.A major QTL controlling seed dormancy and pre-harvest sprouting resistance on chromosome 4Ain a Chinese wheat landrace[J].Molecular Breeding,2008,21(3):351-358.
[6]KATO K,NAKAMURA W,TABIKI T,et al.Detection of loci controlling seed dormancy on group 4chromosomes of wheat and comparative mapping with rice and barley genomes[J].Theoretical&Applied Genetics,2001,102(6-7):980-985.
[7]ROY J K,PRASAD M,VARSHNEY R K,et al.Identification of a microsatellite on chromosomes 6Band a STS on 7Dof bread wheat showing an association with preharvest sprouting tolerance[J].Theoretical&Applied Genetics,1999,99(1-2):336-340.
[8]张海萍,常成,冯继明,等.小麦籽粒休眠Vp1-B1基因的等位变异检测与分离[J].分子植物育种,2009,7(2):297-302.ZHANG H P,CHANG C,FENG J M,et al.Isolation and detection of allelic variations of Vp1-B1gene associated with seed dormancy in Chinese wheat varieties[J].Molecular Plant Breeding,2009,7(2):297-302.
[9]李婷,陈杰,陈锋,等.黄淮麦区地方小麦品种子粒颜色相关基因Tamyb10-1等位变异检测[J].植物遗传资源学报,2014,15(5):1 089-1 095.LI T,CHEN J,CHEN F,et al.Molecular identification of the Tamyb10-1gene controlling the grain color in landrace wheat cultivars from Huanghuai wheat region[J].Journal of Plant Genetic Resources,2014,15(5):1 089-1 095.
[10]LIU S,SEHGAL K,LI J,et al.Cloning and characterization of a critical regulator for preharvest sprouting in wheat[J].Genetics,2013,195(1):263-273.
[11]ZHANG Y,MIAO X,XIA X,et al.Cloning of seed dormancy genes(TaSdr)associated with tolerance to pre-harvest sprouting in common wheat and development of a functional marker[J].Theoretical&Applied Genetics,2014,127(4):855-866.
[12]张春利.小麦抗穗发芽分子标记的发掘与验证[D].北京:中国农业科学院,2008.
[13]YANG Y,ZHAO X L,XIA L Q,et al.Development and validation of a Viviparous-1,STS marker for pre-harvest sprouting tolerance in Chinese wheats.[J].Theoretical&Applied Genetics,2007,115(7):971-980.
[14]杨燕,赵献林,张勇,等.4个小麦抗穗发芽分子抗性标记有效性的验证与评价[J].作物学报,2008,34(1):17-24.YANG Y,ZHAO X L,ZHANG Y,et al.Evaluation and validation of four molecular markers associated with pre-harvest sprouting tolerance in Chinese wheats[J].Acta Agronomica Sinica,2008,34(1):17-24.
[15]刘光辉,杨随庄,黄可兵,等.四川小麦穗发芽抗性鉴定及5个分子标记的有效性检验[J].麦类作物学报,2015,35(2):167-174.LIU G H,YANG S Z,HUANG K B,et al.Identification and validation of five molecular markers for PHS tolerance in Sichuan wheat varieties(lines)[J].Journal of Triticeae Crops,2015,35(2):167-174.
[16]李式昭,郑建敏,伍玲,等.四川小麦品种籽粒硬度和穗发芽抗性相关基因的分子标记鉴定[J].西南农业学报,2018,31(4):641-645.LI S Z,ZHENG J M,WU L,et al.Identification of grain hardness and resistance to pre-harvest sprouting in Sichuan wheat cultivars with molecular markers[J].Southwest China Journal of Agricultural Sciences,2018,31(4):641-645.
[17]郭凤芝,梁伟光,樊庆琦,等.穗发芽相关基因Vp1B3不同等位变异在山东小麦中的分布与演变[J].麦类作物学报,2009,29(4):575-578.GUO F Z,LIANG W G,FAN Q Q,et al.The distribution and evolution of allelic variation of Vp1B3in Shandong wheat[J].Journal of Triticeae Crops,2009,29(4):575-578.
[18]张春利,杨芳萍,宋庆杰,等.黑龙江省小麦品种穗发芽及光周期的分子检测[J].麦类作物学报,2008,28(6):930-934.ZHANG C L,YANG F P,SONG Q J,et al.Moleculer detection of pre-harvest sprouting and photoperiod character in Heilongjiang wheat cultivars[J].Journal of Triticeae Crops,2008,28(6):930-934.
[19]王亮,穆培源,徐红军,等.新疆小麦品种穗发芽Vp-1基因等位变异的分布[J].麦类作物学报,2009,(4):571-574.WANG L,MU P Y,XU H J,et al.Allelic variation of Vp-1gene in Xinjiang wheat cultivars[J].Journal of Triticeae Crops,2009,(4):571-574.
[20]马文洁,张传量,宋晓朋,等.不同麦区小麦品种穗发芽抗性及其与穗部性状的相关性[J].麦类作物学报,2016,36(10):1 269-1 274.MA W J,ZHANG C L,SONG X P,et al.Pre-harvest sprouting resistance in wheat from different wheat regions and its correlation with ear characteristics[J].Journal of Triticeae Crops,2016,36(10):1 269-1 274.
[21]张晓,张伯桥,江伟,等.扬麦系列品种品质性状相关基因的分子检测[J].中国农业科学,2015,48(19):3 779-3 793.ZHANG X,ZHANG B Q,JIANG W,et al.Molecular detection for quality traits-related genes in Yangmai series wheat cultivars[J].Scientia Agricultura Sinica,2015,48(19):3 779-3 793.
[22]刘莉,沈迎芳,马超,等.穗发芽相关基因Vp-1等位变异在西部春小麦品种中的分布研究初探[J].西南农业学报,2014,27(6):2 278-2 281.LIU L,SHEN Y F,MA C,et al.Preliminary study on distribution of allelic variation of Vp-1in western spring wheat varieties[J].Southwest China Journal of Agricultural Sciences,2014,27(6):2 278-2 281.
[23]任立世,刘进英,杨燕.107份小麦历史品种抗穗发芽基因型的检测[J].麦类作物学报,2015,35(6):752-758.REN L S,LIU J Y,YANG Y.Determination of pre-harvest sprouting resistance genotypes with Vp1A3and Vp1B3in107Chinese historical wheat cultivars[J].Journal of Triticeae Crops,2015,35(6):752-758.
[24]MURRAY H G,THOMPSON W F.Rapid isolation of high molecular weight plant DNA[J].Nucleic Acids Research,1980,8(19):4 321-4 325.
[25]朱冬梅,李曼,李东升,等.长江中下游麦区小麦新品种穗发芽抗性及鉴定方法研究[J].核农学报,2018,32(4):795-801.ZHU D M,LI M,LI D S,et al.Evaluation and determination method of pre-harvest sprouting resistance of new wheat cultivars in middle and lower reaches of Yangtze River[J].Journal of Nuclear Agricultural Sciences,2018,32(4):795-801.
[26]曹雪连,张衡,姜昊,等.分子标记PM19-A1对1015份小麦抗穗发芽基因型的筛选及其有效性验证[J].麦类作物学报,2016,36(10):1 283-1 290.CAO X L,ZHANG H,JIANG H,et al.Detection and validation of molecular marker PMl9-A1associated with pre-harvest sprouting resistance in 1015wheat varieties[J].Journal of Triticeae Crops,2016,36(10):1 283-1 290.