原花青素联合维生素E对动脉粥样硬化大鼠的抗氧化作用
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摘要
目的:探讨葡萄籽原花青素(GSP)与VE联合作用对动脉粥样硬化(As)大鼠血脂及抗氧化方面的影响。方法:选用健康雄性Sprague-Dawley(SD)大鼠60只分为空白组和造模组,采用高脂膳食法+腹腔注射VD_3建立As模型。4周后,将造模组分五组:模型组; GSP+VE低、中、高剂量组和阿托伐他汀组,每组10只,灌胃给药干预4周。结束后空白组和模型组各随机抽出2只确认建模成功。测定大鼠的肝脏指数、动脉硬化指数(AI)、血清中血脂水平、内皮舒张因子NO、血清超氧化物歧化酶(SOD)、丙二醛(MDA)的含量及抗超氧阴离子O_2~-、抗羟自由基-OH的能力。结果:与空白组比较,模型组的肝脏指数、AI_1、AI_2、血清TC、TG、LDL-C、MDA的含量均升高(P <0. 05),血清HDL-C的含量、抗O_2~-、-OH的能力、总SOD活力降低(P <0. 05);与模型组相比,GSP+VE低剂量组能改善血清中血脂水平(P <0. 05),而对As的抗氧化作用不显著。相比之下,GSP+VE中、高剂量组的AI_1、AI_2、血清TC、TG、LDL-C、MDA均依次降低(P <0. 05),血清HDL-C的含量、抗O_2~-、-OH的能力、总SOD活力依次升高,可以改善NO的浓度(P <0. 05)。结论:GSP联合VE能通过降血脂及抗氧化作用以延缓As的形成。
Objective: To study the effect of grape seed procyanidins and vitamin E on blood lipid antioxidation in atherosclerotic rats. Methods: 60 healthy male rats were divided into blank groups and modules,establishment of atherosclerosis model by high fat dietary method + intraperitoneal injection of vitamin D_3. After 4 weeks,divide the modules into five groups:model group; GSP + V_E Low,medium and high dosage group and positive control( Atorvastatin) group,10 per group,4 weeks of administration of gastric lavage. After that,the blank group and the model group were randomly drawn 2 to confirm the modeling success. To determine liver index,arteriosclerosis index,serum lipid level,endothelial diastolic factor NO,serum superoxide dismutase,MDA content and the ability of resisting superoxide anion O_2~- and hydroxyl radical -OH. Results: Compared with the blank group,the contents of liver index,AI_1,AI_2,TC,TG,LDL-C and MDA increased in model group( P < 0. 05).The content of serum HDL-C,the ability of resisting O_2~-,and the decrease of T-SOD activity( P < 0. 05); compared with the model group,the GSP + V_E Low dose group can improve serum lipid level( P < 0. 05),the antioxidant effect on as was not significant. In contrast,AI_1,AI_2,TC,TG,LDL-C and MDA of GSP + VEmedium and high group decreased( P < 0. 05),the content of serum HDL-C,the ability of resisting O_2~-,-OH and the activity of T-SOD increased and can improve the concentration of NO( P < 0. 05). Conclusion: GSP combined V_E can retard the formation of atherosclerosis by lowering blood lipids and antioxidation.
Objective: To study the effect of grape seed procyanidins and vitamin E on blood lipid antioxidation in atherosclerotic rats. Methods: 60 healthy male rats were divided into blank groups and modules,establishment of atherosclerosis model by high fat dietary method + intraperitoneal injection of vitamin D_3. After 4 weeks,divide the modules into five groups:model group; GSP + V_E Low,medium and high dosage group and positive control( Atorvastatin) group,10 per group,4 weeks of administration of gastric lavage. After that,the blank group and the model group were randomly drawn 2 to confirm the modeling success. To determine liver index,arteriosclerosis index,serum lipid level,endothelial diastolic factor NO,serum superoxide dismutase,MDA content and the ability of resisting superoxide anion O_2~- and hydroxyl radical -OH. Results: Compared with the blank group,the contents of liver index,AI_1,AI_2,TC,TG,LDL-C and MDA increased in model group( P < 0. 05).The content of serum HDL-C,the ability of resisting O_2~-,and the decrease of T-SOD activity( P < 0. 05); compared with the model group,the GSP + V_E Low dose group can improve serum lipid level( P < 0. 05),the antioxidant effect on as was not significant. In contrast,AI_1,AI_2,TC,TG,LDL-C and MDA of GSP + VEmedium and high group decreased( P < 0. 05),the content of serum HDL-C,the ability of resisting O_2~-,-OH and the activity of T-SOD increased and can improve the concentration of NO( P < 0. 05). Conclusion: GSP combined V_E can retard the formation of atherosclerosis by lowering blood lipids and antioxidation.
引文
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[12]杨光,杜云龙,朱开梅,等.定心藤总黄酮对动脉粥样硬化大鼠血脂及一氧化氮和血管内皮生长因子的影响[J].广东医学,2017,38(9):1309-1313.
[13]高羽,董志.原花青素药理学的研究现状[J].中国中药杂志,2009,24(6):651-655.
[2]薛燕,潍王峰.葡萄籽原花青素预防大鼠动脉粥样硬化作用机制研究[J].中国实用医药,2010,5(13):44-45.
[3]杨宝峰,苏定冯.药理学[M].北京:人民卫生出版社,2016:250-251.
[4]周红,吴晓燕,袁艺标,等.三种剂量维生素D3结合高脂饲料建立大鼠动脉粥样硬化模型的比较[J].中国动脉硬化杂志,2012,20(11):995-998.
[5]仓宝成,宫璀璀,王莉.维生素E在体内外具有抗氧化作用[J].基础医学与临床,2016,36(1):80-84.
[6]余土根,郑敏,方红,等.天然维生素E胶丸治疗黄褐斑有效性与安全性的多中心随机开放临床观察[J].中国皮肤性病学杂志,2012,26(4):367-368.
[7]邱洁,曹爱红,张萍,等.葡萄籽原花青素联合阿托伐他汀治疗老年颈动脉粥样硬化患者的观察[J].中华老年医学杂志,2012,31(5):371-375.
[8]韩磊,叶平,李鸣皋.阿托伐他汀对老年大鼠血清氧化应激水平的影响[J].临床误诊误治,2013,26(7):75-77.
[9]张慢,潘丽军,姜绍通,等.紫薯花青素降血脂及抗氧化效果[J].食品科学,2014,35(19):246-249.
[10]杨小蓉,聂文静,邢军,等.大鼠动脉粥样硬化模型的建立与评价[J].现代预防医学,2016,43(9):1653-1656.
[11]牛春雨,王万铁.病理生理学[M].北京:人民军医出版社,2014:179-180.
[12]杨光,杜云龙,朱开梅,等.定心藤总黄酮对动脉粥样硬化大鼠血脂及一氧化氮和血管内皮生长因子的影响[J].广东医学,2017,38(9):1309-1313.
[13]高羽,董志.原花青素药理学的研究现状[J].中国中药杂志,2009,24(6):651-655.