HS-SPME-GC-MS联用对当归各药用部位挥发油成分的分析
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摘要
目的:分析比较当归各药用部位的挥发油成分。方法:通过单因素考察确定最佳萃取条件,采用顶空固相微萃取法(HS-SPME)与气相色谱-质谱(GC-MS)联用法测定当归各药用部分挥发性化学成分,通过面积归一法确定各成分含量。结果:最终确定条件为称量量0.5 g,萃取温度90℃,萃取时间20 min,解吸附时间3 min。全当归药材粉末出峰94个,当归头药材粉末出峰64个,当归身药材粉末出峰62个,当归尾药材粉末出峰73个,全当归、当归头、当归身和当归尾定性成分含量分别为99.994%,99.747%,97.754%和99.988%。结论:当归挥发性成分主要为藁本内酯且藁本内酯含量:当归身>当归尾>当归头>全当归。
OBJECTIVE To analyze and compare the volatile oil components of various medicinal parts of Angelica.METHODS The optimum extraction conditions were determined by single factor test. Headspace solid-phase microextraction(HS-SPME) was used in combination with gas chromatography-mass spectrometry(GC-MS) to determine the Angelica medicinal parts volatile chemicals. The content of each component was determined by area normalization method.RESULTS The optimum extraction conditions were weighing 0.5 g,extraction temperature 90 ℃,extraction time 20 min,analysis time 3 min. Angelica has 94 peaks with 62 peaks in head parts,62 peaks in body parts,73 peaks in tail parts, The content of qualitative ingredients accounted for 99.994%,99.747%,97.754% and 99.988%,respectively.CONCLUSION The mainly volatile components of Angelica is ligustilide. And the content of ligustilide: body parts > tail parts > head parts> Angelica.
OBJECTIVE To analyze and compare the volatile oil components of various medicinal parts of Angelica.METHODS The optimum extraction conditions were determined by single factor test. Headspace solid-phase microextraction(HS-SPME) was used in combination with gas chromatography-mass spectrometry(GC-MS) to determine the Angelica medicinal parts volatile chemicals. The content of each component was determined by area normalization method.RESULTS The optimum extraction conditions were weighing 0.5 g,extraction temperature 90 ℃,extraction time 20 min,analysis time 3 min. Angelica has 94 peaks with 62 peaks in head parts,62 peaks in body parts,73 peaks in tail parts, The content of qualitative ingredients accounted for 99.994%,99.747%,97.754% and 99.988%,respectively.CONCLUSION The mainly volatile components of Angelica is ligustilide. And the content of ligustilide: body parts > tail parts > head parts> Angelica.
引文
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[11] Chen ZH,Wu GT,Sun M, et al. An overview of the research on Angelica sinensis [J]. J Gansu Univ Chin Med(甘肃中医药大学学报), 2018,35(02):102-105.
[12] Dong Q,Chen MC. Chemical Composition and Pharmacological Research Progress of Angelica [J]. Asia-Pacific Tradit Med (亚太传统医药), 2016, 12(2):32-34.
[13] Fan WT,Wang LS,Fan YM, et al. Buyang huanwu Tang protects rat brain microvascular endothelial cells injured by oxygen-glucose deprivation-reperfusion [J]. Chin J Exp Med Form(中国实验方剂学杂志),2016,22(14):111-115.
[14] Zheng HZ,Wang LS,Meng YJ. Fffects of Buyang Huanwu Decoction against oxidative stress of rBMECs by up-regulating SIRTI [J]. Pharmocol Clin Chin Mater Med (中药药理与临床),2017,33(02):9-13.
[15] Shen X,Zheng HZ,Meng YJ et al. UPLC-Q-TOF-MS/MS Analysis of brain and blood absorption components of Buyang Huanwu Tang after intragastric administration [J]. Chin J Exp Med Form (中国实验方剂学杂志),2019,25(2):8-13.
[2] Fu RN. Development of solidphase microextraction (SPME) in recent years [J]. Chin J Anal Lab(分析试验室), 2015, 34(5):602-620.
[3] Ch.P.Vol Ⅰ(中国药典.一部)[S].2015: 151.
[4] Zhang Q,Ru QG,Lin HM, et al. GC-MS analysis and antioxidant activity study of distillates separated from essential oil of Angelicae Sinensis Radix by molecular distillation [J]. Chin J Inform on Tradit Chin Med(中国中医药信息杂志), 2016, 23(2):82-87.
[5] Xiao MF. Current research situation of Buyang Huanwu Decoction and its development of new medical invention [J].Chin Tradit Herbal Drugs(中草药), 2018(7):1688-1694.
[6] Huang X,Li YF,Kou GJ, et al. Research progress of mechanism of anti-atherosclerosis of Buyang Huanwu Decoction [J]. Chin J Tradit Chin Med Pharm (中华中医药杂志), 2017(3):1187-1190.
[7] Hu CY,Ding XS. Extraction, isolation and structure identification of ligustilide from Angelica sinensis [J]. J Wuxi Univ Light Indust(食品与生物技术学报), 2003, 22(5):69-71.
[8] Wu HY,Hua YL,Guo DS, et al. Extraction and analysis of volatile oil in different parts of Radix Angelicae sinensis from min county in Gansu [J]. Natural Product Res Develop (天然产物研究与开发), 2012, 24(9):1225-1229.
[9] Meng D. Analysis of etiology and pathogenesis of stroke in traditional Chinese medicine [J]. Sci Technol View(科技视界), 2016(18):165-165.
[10] Zhou H,Huang HH,Zhang JZ, et al. Research progress of Chuanxiong-Danggui medicine [J]. Chin Tradit Patent Med(中成药), 2015, 37(1):184-188.
[11] Chen ZH,Wu GT,Sun M, et al. An overview of the research on Angelica sinensis [J]. J Gansu Univ Chin Med(甘肃中医药大学学报), 2018,35(02):102-105.
[12] Dong Q,Chen MC. Chemical Composition and Pharmacological Research Progress of Angelica [J]. Asia-Pacific Tradit Med (亚太传统医药), 2016, 12(2):32-34.
[13] Fan WT,Wang LS,Fan YM, et al. Buyang huanwu Tang protects rat brain microvascular endothelial cells injured by oxygen-glucose deprivation-reperfusion [J]. Chin J Exp Med Form(中国实验方剂学杂志),2016,22(14):111-115.
[14] Zheng HZ,Wang LS,Meng YJ. Fffects of Buyang Huanwu Decoction against oxidative stress of rBMECs by up-regulating SIRTI [J]. Pharmocol Clin Chin Mater Med (中药药理与临床),2017,33(02):9-13.
[15] Shen X,Zheng HZ,Meng YJ et al. UPLC-Q-TOF-MS/MS Analysis of brain and blood absorption components of Buyang Huanwu Tang after intragastric administration [J]. Chin J Exp Med Form (中国实验方剂学杂志),2019,25(2):8-13.