正天丸对偏头痛模型大鼠三叉神经颈髓复合体c-fos、细胞外调节蛋白激酶表达的影响
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摘要
目的:观察正天丸对硝酸甘油致偏头痛模型大鼠三叉神经颈髓复合体c-fos、细胞外调节蛋白激酶(ERK)表达的影响。方法:将30只雄性SD大鼠按照随机数字表法分为空白组、模型组和治疗组。治疗组根据体表换算预防性给予正天丸灌胃,模型组和空白组给予相同剂量的生理盐水灌胃,连续7 d。模型组和治疗组采用皮下注射NTG致大鼠偏头痛模型,空白组给予生理盐水皮下注射。观察各组大鼠耳红时间、持续挠头时间和爬笼次数,采用免疫荧光法和蛋白质印迹法检测各组大鼠三叉神经颈髓复合体中c-fos、ERK表达水平。结果:与模型组相比,治疗组耳红持续时间、持续挠头时间和爬笼次数均明显减少。c-fos和ERK在硝酸甘油诱发的偏头痛大鼠的三叉神经颈髓复合体有较高的表达。与空白组比较,模型组大鼠三叉神经颈髓复合体中ERK表达水平明显升高(P<0.05);治疗组大鼠ERK表达明显低于模型组(P<0.05)。结论:正天丸可通过抑制偏头痛模型大鼠三叉神经颈髓复合体中c-fos、ERK的表达发挥降低偏头痛疼痛的作用。
引文
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[2]EDVINSSON L. Headache advances in 2017: a new horizon in migraine therapy[J]. Lancet Neurol, 2018, 17(1): 5-6.
[3]MALONE C D, BHOWMICK A, WACHHOLTZ A B. Migraine: treatments, comorbidities, and quality of life, in the USA[J]. J Pain Res, 2015, 8(1): 537-547.
[4]BHATT D K, RAMACHANDRAN R, CHRISTENSEN S L, et al. CGRP infusion in unanesthetized rats increases expression of c-fos in the nucleus tractus solitarius and caudal ventrolateral medulla, but not in the trigeminal nucleus caudalis[J]. Cephalalgia, 2015, 35(3): 220-233.
[5]付昆,付文君,魏江平,等.正天丸的镇痛作用及机制研究[J].中国中医基础医学杂志,2017,23(3):401-404.
[6]陈倢,梁伟雄,秦劭晨,等.正天丸治疗偏头痛的有效性与安全性的系统评价[J].中国实验方剂学杂志,2015,21(19):194-200.
[7]王勇,袁灿兴,商洪才,等.正天丸治疗偏头痛随机对照双盲双模拟多中心临床研究[J].中成药,2012,34(5):791-794.
[8]PARK S H,LEE J R,JANG S P,et al.Antinociceptive profiles and mechanisms of centrally administered oxyntomodulin in various mouse pain models[J]. Neuropeptides,2018,68(4):7-14..
[9]DING S, FSA A. P2X3 receptor involvement in endometriosis pain via ERK signaling pathway[J].PloS one,2017,12(9): e0184647.
[10]HUANG W J, WANG B R, YAO L B,et al.Activity of p44/42 MAP kinase in the caudal subnucleus of trigeminal spinal nucleus is increased following perioral noxious stimulation in the mouse[J]. Brain research,2000,861(1):181-185.
[11]赵丽娟,牛争平.ERK在偏头痛动物模型中的激活及与肥大细胞脱颗粒间的关系[J].中西医结合心脑血管病杂志,2011,9(5):587-589.
[12]VALENCA M M. Commentary: the effect of systemic nitroglycerin administration on the kynurenine pathway in the rat[J]. Front Neurol,2017,8(1): 518.
[13]STEPIEN T, TARACHA E, KANIUGA E,et al. Effects of amphetamine administration on neurogenesis in adult rats[J]. Folia Neuropathol,2017,55(4): 333-339.
[14]RAMACHANDRAN R, BHATT D K, PLOUG K B,et al. Nitric oxide synthase, calcitonin gene-related peptide and NK-1 receptor mechanisms are involved in GTN-induced neuronal activation[J]. Cephalalgia, 2014,34(2):136-147.