氢气植物学效应的研究进展
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摘要
氢气(hydrogen,H_2)是一种新的气体信号分子。藻类和微生物的氢酶可以产生并释放H_2,高等植物也可能存在类似于氢酶的蛋白催化H_2的合成。同时,各种非生物胁迫和激素也可诱导产生H_2。与动物中H_2通过选择性抗氧化预防和辅助治疗多种疾病所不同的是,植物中H_2的生理作用可能还与其通过迅速诱导活性氧信号,从而调动抗氧化防护系统有关。H_2具有多种植物学效应,包括调控生长发育和提高对生物胁迫和非生物胁迫的抗/耐性,其分子机制涉及对miRNA、基因表达、激素水平和蛋白质修饰的调控,并可能与一氧化氮(nitric oxide,NO)和一氧化碳(carbon monoxide,CO)等气体信号转导有关。H_2的研究掀起了新一轮健康革命,"氢农业"的发展也已初具规模。本文主要综述了H_2产生、生理功能以及与激素等信号分子互作的研究进展,并对H_2植物学效应和"氢农业"进行了展望。
Hydrogen(H_2) is a new gas signal molecule,and hydrogen enzymes from the algae and microorganism can produce and release H_2. Higher plants may have hydrogen-like protein(s) that catalyze the production of H_2,and the production of H_2 is also induced by abiotic stresses and phytohormones. As a novel antioxidant,H_2 has been proven effective in adjutant therapy. Importantly,the role of H_2 in plants may also be related to the first rapidly induced reactive oxygen species signals,thus triggering antioxidant defense. H_2 plays multiple biological roles,including regulating plant growth,development,and increasing plant tolerance/resistance against abiotic and biotic stresses; its molecular mechanisms involve in the regulation of miRNA,gene expression,phytohormone levels,and protein modification. In addition,nitric oxide(NO) and carbon monoxide(CO) are involved in the biological effects induced by H_2. Recently,researches of H_2 had set off a new round of healthy revolution,and the development of hydrogen agriculture was just a beginning. This paper mainly reviewed the research progress of H_2 production,physiological functions,and interactions with hormones and other signaling molecules. Finally,the prospect of botanical effects of H_2 and hydrogen agriculture was suggested.
Hydrogen(H_2) is a new gas signal molecule,and hydrogen enzymes from the algae and microorganism can produce and release H_2. Higher plants may have hydrogen-like protein(s) that catalyze the production of H_2,and the production of H_2 is also induced by abiotic stresses and phytohormones. As a novel antioxidant,H_2 has been proven effective in adjutant therapy. Importantly,the role of H_2 in plants may also be related to the first rapidly induced reactive oxygen species signals,thus triggering antioxidant defense. H_2 plays multiple biological roles,including regulating plant growth,development,and increasing plant tolerance/resistance against abiotic and biotic stresses; its molecular mechanisms involve in the regulation of miRNA,gene expression,phytohormone levels,and protein modification. In addition,nitric oxide(NO) and carbon monoxide(CO) are involved in the biological effects induced by H_2. Recently,researches of H_2 had set off a new round of healthy revolution,and the development of hydrogen agriculture was just a beginning. This paper mainly reviewed the research progress of H_2 production,physiological functions,and interactions with hormones and other signaling molecules. Finally,the prospect of botanical effects of H_2 and hydrogen agriculture was suggested.
引文
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[14]Khanna N,Lindblad P.Cyanobacterial hydrogenases and hydrogen metabolism revisited:recent progress and future prospects[J].Int J Mol Sci,2015,16(5):10537-10561.
[15]Meyer J,Kelley B C,Vignais P M.Effect of light nitrogenase function and synthesis in Rhodopseudomonas capsulate[J].J Bacteriol,1978,136(1):201-208.
[16]Florin L,Tsokoglou A,Happe T.A novel type of iron hydrogenase in the green alga Scenedesmus obliquus is linked to the photosynthetic electron transport chain[J].J Biol Chem,2001,276(9):6125-6132.
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[19]Boychenko E A.Hydrogenase of isolated chloroplasts[J].Biokhimiya,1947,12(2):153-162.
[20]Renwick G M,Giumarro C,Siegel S M.Hydrogen metabolism in higher plants[J].Plant Physiol,1964,39(3):303-306.
[21]Torres V,Ballesteros A,Fernandez V M.Expression of hydrogenase activity in barley(Hordeum vulgare L.)after anaerobic stress[J].Arch Biochem Biophys,1986,245(1):174-178.
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[23]Zeng J,Zhang M,Sun X.Molecular hydrogen is involved in phytohormone signaling and stress responses in plants[J].PLoS One,2013,8:e71038.
[24]Xie Y,Mao Y,Lai D,et al.H2enhances Arabidopsis salt tolerance by manipulating ZAT10/12-mediated antioxidant defence and controlling sodium exclusion[J].PLoS One,2012,7:e49800.
[25]Jin Q,Cui W,Dai C,et al.Involvement of hydrogen peroxide and heme oxygenase-1 in hydrogen gas-induced osmotic stress tolerance in alfalfa[J].Plant Growth Regul,2016,80(2):215-223.
[26]Jin Q,Zhu K,Cui W,et al.Hydrogen-modulated stomatal sensitivity to abscisic acid and drought tolerance via the regulation of apoplastic p H in Medicago sativa[J].J Plant Growth Regul,2016,35:565-573.
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[28]Xie Y,Mao Y,Zhang W,et al.Reactive oxygen species-dependent nitric oxide production contributes to hydrogen-promoted stomatal closure in Arabidopsis[J].Plant Physiol,2014,165(2):759-773.
[29]Cao Z,Duan X,Yao P,et al.Hydrogen gas is involved in auxin-induced lateral root formation by modulating nitric oxide synthesis[J].Int J Mol Sci,2017,18(10):2084.
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