裂褶多糖制备及化学改性研究进展
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摘要
裂褶菌(Schizophyllum commune)是一种食药兼用真菌,裂褶多糖(Schizophyllan,SPG)是裂褶菌的主要活性成分之一,SPG在医药、化妆品、食品等领域有着广阔的应用前景,但国内尚未实现SPG原料的商业化开发。本文综述SPG在生物发酵法制备、分离工艺选择、化学改性及应用方面的进展,发现SPG作为免疫药物载体、生物医学材料是当前热点研究和应用领域;指出了当前研究中存在的含量测定方法不统一等问题,针对SPG的研发和商业化应用提出了建议。
Schizophyllum commune is a kind of edible and medicinal fungi. Schizophyllan(SPG) is one of the main active components of Schizophyllum commune,which has a broad application prospects in medicine,cosmetics and food. However,the commercial development of SPG has not been realized in China. This paper reviews the progress of SPG in preparation by fermentation,separation process selection,chemical modification and application,and finds that SPG,as an immunopharmaceutical carrier and biomedical material,becomes the hot research and application fields. This paper also points out the problems existing in the current research,such as the lack of uniform method for the determination of SPG,and puts forward some suggestions for the research and development and commercial application of SPG.
Schizophyllum commune is a kind of edible and medicinal fungi. Schizophyllan(SPG) is one of the main active components of Schizophyllum commune,which has a broad application prospects in medicine,cosmetics and food. However,the commercial development of SPG has not been realized in China. This paper reviews the progress of SPG in preparation by fermentation,separation process selection,chemical modification and application,and finds that SPG,as an immunopharmaceutical carrier and biomedical material,becomes the hot research and application fields. This paper also points out the problems existing in the current research,such as the lack of uniform method for the determination of SPG,and puts forward some suggestions for the research and development and commercial application of SPG.
引文
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[3] SINGH M K,KUMAR M,THAKUR I S. Proteomic characterization and Schizophyllan production by Schizophyllum commune ISTL04 cultured on Leucaena Leucocephala wood under submerged fermentation[J]. Bioresource Technol,2017,236:29-36
[4] JAMSHIDIAN H,SHOJAOSADATI S A,VILAPLANA F,et al. Characterization and optimization of Schizophyllan production from date syrup[J]. Int J Biol Macromol,2016,92:484-493
[5] SUTIVISEDSAK N,LEATHERS T D,NUNNALLY M S,et al. Utilization of agricultural biomass in the production of the biopolymer Schizophyllan[J]. J Ind Microbiol Biotechnol,2013,40(1):105-112.
[6] 孟秀梅,李明华. 裂褶菌深层发酵多糖的闪式提取[J]. 食品工业,2013(10):75-78.
[7] 崔信旭,黄淳锡,石地琄. 裂褶菌菌丝球的制造方法及利用其的β-葡聚糖生产方法:CN104673674A[P].2015-06-03.
[8] 周莹,李兴红,严红,等.裂褶菌液体培养条件的初步研究[J].中国农学通报,2012,28(01):187-190.
[9] LI Wenbing,ZHOU Pengpeng,YU Longjiang. Statistical optimization of the medium composition by response surface methodology to enhance Schizophyllan production by Schizophyllum commune[J]. Zeitschrift Fur Naturforschung Section C-A J Biosci,2011,66:173-181.
[10] 郑文科,乔长晟,郝利民,等.四种生长因子对裂褶菌胞外多糖产量影响的优化研究[J].食品工业科技,2009,30(5):181-183,187.
[11] KIM M S,PARK Y D,LEE S R. Method of using beta-glucan from schizophyllum commune:US 20090023681 A1[P]. 2009.
[12] 张虽栓,蔡花真.高分子量裂褶多糖发酵培养工艺的正交优化[J].食品研究与开发,2013,34(11):42-44,100.
[13] 杨娜,王鸿飞,宋佳敏,等.超声波辅助提取裂褶菌多糖及分离纯化的研究[J].核农学报,2014,28(11):2015-2024.
[14] 杨娜,王鸿飞,郝艳佳,等.裂褶菌多糖提取工艺及抗氧化活性研究[J].中国食品学报,2014,14(8):92-98.
[15] 陈聪,钟葵,林伟静,等.固体发酵裂褶菌多糖提取方法比较[J].核农学报,2011,25(6):1211-1215.
[16] 钟昕,周素梅,王强,等.辐照对发酵玉米芯中裂褶菌多糖提取及流变学性质的影响[J].核农学报,2010,24(1):67-72.
[17] 王振河,万学东,武忠伟,等.正交设计优化裂褶菌发酵全液多糖提取工艺[J].中国野生植物资源,2007(4):50-51+56.
[18] 周林,郑必胜,郭祀远,等.裂褶多糖的羧甲基化(英文)[J].天然产物研究与开发,2006(4):555-557.
[19] 郑必胜,胡康,周立武,等.羧甲基裂褶多糖的制备及其保湿活性研究[J].现代食品科技,2017,33(8):254-261.
[20] YOSHIBA K,SATO T,OSUMI T,et al. Conformation of carboxy-lated Schizophyllan in aqueous solution[J]. Carbohydr Polym,2015,134:1-5.
[21] YOSHIBA K,OKAMOTO S,DOBASHI T,et al. Effects of carboxy-lation of the side chains on the order-disorder transition in aqueous solution of Schizophyllan,a triple helical polysaccharide[J]. Carbohydr Polym,2017,168:79.
[22] 冯小玲,方兆华,刘建欣,等.裂褶多糖磷酸化衍生物的制备与性能研究[J].日用化学工业,2017,47(11):637-640.
[23] 司丽娜. 裂褶菌胞外多糖的硫酸化修饰及红外表征[J]. 食品安全导刊,2015(12):99-100.
[24] 张虽栓,蔡花真. 硫酸酯化裂褶菌多糖的制备及其抗氧化活性研究[J]. 食品研究与开发,2017,38(8):17-21. 1.
[25] MAEDA K,MOCHIZUKI S,SANADA Y,et al. Naphthalene- hydrophobized β-1,3-glucan nanogel for doxorubicin delivery to immunocytes[J]. Bioorg Med Chem Lett,2014,24:1880-1883.
[26] MOCHIZUKI S,MORISHITA H,SAKURAI K. Macrophage specific delivery of TNF-α siRNA complexed with β-1,3-glucan inhibits LPS-induced cytokine production in a murine acute hepatitis model[J]. Bioorg Med Chem,2013,21(9):2535-2542.
[27] MOCHIZUKI S,MORISHITA H,ADACHI Y,et al. Binding assay between murine Dectin-1 and β-glucan/DNA complex with quartz-crystal microbalance[J]. Carbohydr Res,2014,391:1-8.
[28] KOBIYAMA K,AOSHI T,NARITA H,et al. Nonagonistic Dectin-1 ligand transforms CpG into a multitask nanoparticulate TLR9 agonist[J]. Proc Natl Acad Sci USA,2014,111(8):3086.
[29] ZHANG Q,ICHIMARU N,HIGUCHI S,et al. Permanent accep-tance of mouse cardiac allografts with CD40 siRNA to induce regulatory myeloid cells by use of a novel polysaccharide siRNA delivery system[J]. Gene Ther,2015,22:1-10.
[30] KIM Y R,HWANG Jiangsun,KOH H J,et al. The targeted delivery of the c-Src peptide complexed with schizophyllan to macrophages inhibits polymicrobial sepsis and ulcerative colitis in mice[J]. Biomater,2016,89:1-13.
[31] KITAHATA Y,KANUMA T,HAYASHI M,et al. Circulating nano-particulate TLR9 agonist scouts out tumor microenvironment to release immunogenic dead tumor cells[J]. Oncotarget,2016,7(31):48860-48869.
[32] IZUMI H,NAGAO S,MOCHIZUKI S,et al. Optimal sequence of antisense DNA to silence YB-1 in lung cancer by use of a novel polysaccharide drug delivery system[J]. Int J Clin Oncol,2016,48(6):2472.
[33] KOBIYAMA K,TEMIZOZ B,KANUMA T,et al. Species-dependent role of type I IFNs and IL-12 in the CTL response induced by humanized CpG complexed with β-glucan[J]. EUR J Immunol,2016,46(5):1142-1151.
[34] YOKOKAWA H,HIGASHINO A,SUZUKI S,et al. Induction of humoural and cellular immunity by immunisation with HCV particle vaccine in a non-human primate model[J]. Gut,2016,67(2):gutjnl-2016-312208
[35] ITO H,ANDO T,NAKAMURA M,et al. Induction of humoral and cellular immune response to hepatitis B virus (HBV) vaccine can be upregulated by CpG oligonucleotides complexed with Dectin-1 ligand[J]. J Viral Hep,2016,24(2):155
[36] MOCHIZUKI S,MORISHITA H,SAKURAI K. Complex consisting of β-glucan and antigenic peptides with cleavage site for glutathione and aminopeptidases induces potent cytotoxic T lymphocytes[J]. Bioconjug Chem,2017,28(9):2246-2253.
[37] MIYAMOTO N,MOCHIZUKI S,SAKURAI K. Designing an immunocyte-targeting delivery system by use of beta-glucan[J]. Vaccine,2018,36(1):186-189.
[38] MASUTA Y,YAMAMOTO T,NATSUMEKITATANI Y,et al. An antigen-free,plasmacytoid dendritic cell-targeting immunotherapy to bolster memory CD8+ T cells in nonhuman Primates[J]. J Immunol,2018,200(6):ji1701183.
[39] FUJIWARA N. Complex consisting of antisense DNA and β-glucan promotes internalization into cell through Dectin-1 and hybridizes with target mRNA in cytosol[J]. Cancer Gene Ther,2018.
[40] LEE S H,PARK K M,LEE S H,et al. Moisturizing cosemetics compostion containing beta-1,6-branched-beta-1,3-glucan:KR19990004497[P].1999
[41] LEE G S,BAE J T,SONG S K,et al. cosmetic composition containing fermented herb extracts:KR20110002578[P].2011.
[42] 周莹,李兴红,燕继晔,等. 裂褶菌多糖在防治烟草花叶病毒病上的应用:CN102265830A[P]2011.
[43] ABDEL-MOHSEN A M,ABDEL-RAHMAN R M,FOUDA M M G,et al. Preparation,characterization and cytotoxicity of schizophyllan/ silver nanoparticle composite[J]. Carbohydr Polym,2014,102(102):238-245.
[44] 赵岳,杨娜,沈晓俊,等.响应面优化裂褶菌多糖酶解工艺及抑菌研究[J].菌物学报,2015,34(1):139-149.
[45] ABDELMOHSEN A M,JANCAR J,MASSOUD D,et al. Novel chitin/chitosan-glucan wound dressing:isolation,characterization,antibacterial activity and wound healing properties[J]. Int J Pharm,2016,510(1):86-99.
[46] LEE S,TAE H,KI C S. Fabrication of schizophyllan hydrogel via orthogonal thiol-ene photopolymerization[J]. Carbohydr Polym,2017,167:270-279
[47] SUTIVISEDSAK N,LEATHERS T D,BIRESAW G,et al. Simplified process for preparation of schizophyllan solutions for biomaterial applications[J]. Prep Biochem,2016,46(3):313-319.
[48] JOSEWSKI J,BUCHMEIER S,FRENZEL A,et al. Generation of recombinant antibodies against the beta-(1,6)-branched beta-(1,3)-D-glucan schizophyllan from immunized mice via phage display[J]. Biotechnol Res Int,2017(2):1-8.
[2] 贺凤,黄龙花,刘远超,等. 裂褶菌多糖的研究进展[J]. 食用菌学报,2016,23(2):88-93.
[3] SINGH M K,KUMAR M,THAKUR I S. Proteomic characterization and Schizophyllan production by Schizophyllum commune ISTL04 cultured on Leucaena Leucocephala wood under submerged fermentation[J]. Bioresource Technol,2017,236:29-36
[4] JAMSHIDIAN H,SHOJAOSADATI S A,VILAPLANA F,et al. Characterization and optimization of Schizophyllan production from date syrup[J]. Int J Biol Macromol,2016,92:484-493
[5] SUTIVISEDSAK N,LEATHERS T D,NUNNALLY M S,et al. Utilization of agricultural biomass in the production of the biopolymer Schizophyllan[J]. J Ind Microbiol Biotechnol,2013,40(1):105-112.
[6] 孟秀梅,李明华. 裂褶菌深层发酵多糖的闪式提取[J]. 食品工业,2013(10):75-78.
[7] 崔信旭,黄淳锡,石地琄. 裂褶菌菌丝球的制造方法及利用其的β-葡聚糖生产方法:CN104673674A[P].2015-06-03.
[8] 周莹,李兴红,严红,等.裂褶菌液体培养条件的初步研究[J].中国农学通报,2012,28(01):187-190.
[9] LI Wenbing,ZHOU Pengpeng,YU Longjiang. Statistical optimization of the medium composition by response surface methodology to enhance Schizophyllan production by Schizophyllum commune[J]. Zeitschrift Fur Naturforschung Section C-A J Biosci,2011,66:173-181.
[10] 郑文科,乔长晟,郝利民,等.四种生长因子对裂褶菌胞外多糖产量影响的优化研究[J].食品工业科技,2009,30(5):181-183,187.
[11] KIM M S,PARK Y D,LEE S R. Method of using beta-glucan from schizophyllum commune:US 20090023681 A1[P]. 2009.
[12] 张虽栓,蔡花真.高分子量裂褶多糖发酵培养工艺的正交优化[J].食品研究与开发,2013,34(11):42-44,100.
[13] 杨娜,王鸿飞,宋佳敏,等.超声波辅助提取裂褶菌多糖及分离纯化的研究[J].核农学报,2014,28(11):2015-2024.
[14] 杨娜,王鸿飞,郝艳佳,等.裂褶菌多糖提取工艺及抗氧化活性研究[J].中国食品学报,2014,14(8):92-98.
[15] 陈聪,钟葵,林伟静,等.固体发酵裂褶菌多糖提取方法比较[J].核农学报,2011,25(6):1211-1215.
[16] 钟昕,周素梅,王强,等.辐照对发酵玉米芯中裂褶菌多糖提取及流变学性质的影响[J].核农学报,2010,24(1):67-72.
[17] 王振河,万学东,武忠伟,等.正交设计优化裂褶菌发酵全液多糖提取工艺[J].中国野生植物资源,2007(4):50-51+56.
[18] 周林,郑必胜,郭祀远,等.裂褶多糖的羧甲基化(英文)[J].天然产物研究与开发,2006(4):555-557.
[19] 郑必胜,胡康,周立武,等.羧甲基裂褶多糖的制备及其保湿活性研究[J].现代食品科技,2017,33(8):254-261.
[20] YOSHIBA K,SATO T,OSUMI T,et al. Conformation of carboxy-lated Schizophyllan in aqueous solution[J]. Carbohydr Polym,2015,134:1-5.
[21] YOSHIBA K,OKAMOTO S,DOBASHI T,et al. Effects of carboxy-lation of the side chains on the order-disorder transition in aqueous solution of Schizophyllan,a triple helical polysaccharide[J]. Carbohydr Polym,2017,168:79.
[22] 冯小玲,方兆华,刘建欣,等.裂褶多糖磷酸化衍生物的制备与性能研究[J].日用化学工业,2017,47(11):637-640.
[23] 司丽娜. 裂褶菌胞外多糖的硫酸化修饰及红外表征[J]. 食品安全导刊,2015(12):99-100.
[24] 张虽栓,蔡花真. 硫酸酯化裂褶菌多糖的制备及其抗氧化活性研究[J]. 食品研究与开发,2017,38(8):17-21. 1.
[25] MAEDA K,MOCHIZUKI S,SANADA Y,et al. Naphthalene- hydrophobized β-1,3-glucan nanogel for doxorubicin delivery to immunocytes[J]. Bioorg Med Chem Lett,2014,24:1880-1883.
[26] MOCHIZUKI S,MORISHITA H,SAKURAI K. Macrophage specific delivery of TNF-α siRNA complexed with β-1,3-glucan inhibits LPS-induced cytokine production in a murine acute hepatitis model[J]. Bioorg Med Chem,2013,21(9):2535-2542.
[27] MOCHIZUKI S,MORISHITA H,ADACHI Y,et al. Binding assay between murine Dectin-1 and β-glucan/DNA complex with quartz-crystal microbalance[J]. Carbohydr Res,2014,391:1-8.
[28] KOBIYAMA K,AOSHI T,NARITA H,et al. Nonagonistic Dectin-1 ligand transforms CpG into a multitask nanoparticulate TLR9 agonist[J]. Proc Natl Acad Sci USA,2014,111(8):3086.
[29] ZHANG Q,ICHIMARU N,HIGUCHI S,et al. Permanent accep-tance of mouse cardiac allografts with CD40 siRNA to induce regulatory myeloid cells by use of a novel polysaccharide siRNA delivery system[J]. Gene Ther,2015,22:1-10.
[30] KIM Y R,HWANG Jiangsun,KOH H J,et al. The targeted delivery of the c-Src peptide complexed with schizophyllan to macrophages inhibits polymicrobial sepsis and ulcerative colitis in mice[J]. Biomater,2016,89:1-13.
[31] KITAHATA Y,KANUMA T,HAYASHI M,et al. Circulating nano-particulate TLR9 agonist scouts out tumor microenvironment to release immunogenic dead tumor cells[J]. Oncotarget,2016,7(31):48860-48869.
[32] IZUMI H,NAGAO S,MOCHIZUKI S,et al. Optimal sequence of antisense DNA to silence YB-1 in lung cancer by use of a novel polysaccharide drug delivery system[J]. Int J Clin Oncol,2016,48(6):2472.
[33] KOBIYAMA K,TEMIZOZ B,KANUMA T,et al. Species-dependent role of type I IFNs and IL-12 in the CTL response induced by humanized CpG complexed with β-glucan[J]. EUR J Immunol,2016,46(5):1142-1151.
[34] YOKOKAWA H,HIGASHINO A,SUZUKI S,et al. Induction of humoural and cellular immunity by immunisation with HCV particle vaccine in a non-human primate model[J]. Gut,2016,67(2):gutjnl-2016-312208
[35] ITO H,ANDO T,NAKAMURA M,et al. Induction of humoral and cellular immune response to hepatitis B virus (HBV) vaccine can be upregulated by CpG oligonucleotides complexed with Dectin-1 ligand[J]. J Viral Hep,2016,24(2):155
[36] MOCHIZUKI S,MORISHITA H,SAKURAI K. Complex consisting of β-glucan and antigenic peptides with cleavage site for glutathione and aminopeptidases induces potent cytotoxic T lymphocytes[J]. Bioconjug Chem,2017,28(9):2246-2253.
[37] MIYAMOTO N,MOCHIZUKI S,SAKURAI K. Designing an immunocyte-targeting delivery system by use of beta-glucan[J]. Vaccine,2018,36(1):186-189.
[38] MASUTA Y,YAMAMOTO T,NATSUMEKITATANI Y,et al. An antigen-free,plasmacytoid dendritic cell-targeting immunotherapy to bolster memory CD8+ T cells in nonhuman Primates[J]. J Immunol,2018,200(6):ji1701183.
[39] FUJIWARA N. Complex consisting of antisense DNA and β-glucan promotes internalization into cell through Dectin-1 and hybridizes with target mRNA in cytosol[J]. Cancer Gene Ther,2018.
[40] LEE S H,PARK K M,LEE S H,et al. Moisturizing cosemetics compostion containing beta-1,6-branched-beta-1,3-glucan:KR19990004497[P].1999
[41] LEE G S,BAE J T,SONG S K,et al. cosmetic composition containing fermented herb extracts:KR20110002578[P].2011.
[42] 周莹,李兴红,燕继晔,等. 裂褶菌多糖在防治烟草花叶病毒病上的应用:CN102265830A[P]2011.
[43] ABDEL-MOHSEN A M,ABDEL-RAHMAN R M,FOUDA M M G,et al. Preparation,characterization and cytotoxicity of schizophyllan/ silver nanoparticle composite[J]. Carbohydr Polym,2014,102(102):238-245.
[44] 赵岳,杨娜,沈晓俊,等.响应面优化裂褶菌多糖酶解工艺及抑菌研究[J].菌物学报,2015,34(1):139-149.
[45] ABDELMOHSEN A M,JANCAR J,MASSOUD D,et al. Novel chitin/chitosan-glucan wound dressing:isolation,characterization,antibacterial activity and wound healing properties[J]. Int J Pharm,2016,510(1):86-99.
[46] LEE S,TAE H,KI C S. Fabrication of schizophyllan hydrogel via orthogonal thiol-ene photopolymerization[J]. Carbohydr Polym,2017,167:270-279
[47] SUTIVISEDSAK N,LEATHERS T D,BIRESAW G,et al. Simplified process for preparation of schizophyllan solutions for biomaterial applications[J]. Prep Biochem,2016,46(3):313-319.
[48] JOSEWSKI J,BUCHMEIER S,FRENZEL A,et al. Generation of recombinant antibodies against the beta-(1,6)-branched beta-(1,3)-D-glucan schizophyllan from immunized mice via phage display[J]. Biotechnol Res Int,2017(2):1-8.