釉原蛋白在牙周组织再生中的生物学作用
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摘要
牙周组织缺损的修复是牙周治疗的一大难题,目前釉基质蛋白被广泛用于牙周组织再生治疗,并取得了良好的治疗效果。釉原蛋白是釉基质蛋白的主要成分,在牙周组织再生中发挥着多种生物学作用,调节牙周组织细胞的生物学行为,进而影响牙周组织再生的过程。目前对于釉原蛋白引起牙周组织再生的生物学机制仍不清楚,其作用的信号通路仍有待研究。本文将从调节细胞的黏附、迁移、增殖、分化等方面,论述釉原蛋白在牙周组织再生中的作用,探讨其可能的作用机制。
Recovery of the lost bone tissue of periodontitis patients is the ultimate purpose of periodontic clinical treatment. Enamel matrix proteins(EMPs) are widely used in periodontal regeneration treatment and lead to an appealing outcome. Amelogenin(AML) is the major component of EMPs with multiple biological effects on periodontal regeneration progress. However, the mechanism of this progress is still unclear. The signal pathway by which AML may be triggered during bone regeneration is still under investigation. In this study, we attempt to discuss the function of AML in cell attachment, migration, proliferation and differentiation during periodontal regeneration and try to explain possible mechanisms underlying such progress.
Recovery of the lost bone tissue of periodontitis patients is the ultimate purpose of periodontic clinical treatment. Enamel matrix proteins(EMPs) are widely used in periodontal regeneration treatment and lead to an appealing outcome. Amelogenin(AML) is the major component of EMPs with multiple biological effects on periodontal regeneration progress. However, the mechanism of this progress is still unclear. The signal pathway by which AML may be triggered during bone regeneration is still under investigation. In this study, we attempt to discuss the function of AML in cell attachment, migration, proliferation and differentiation during periodontal regeneration and try to explain possible mechanisms underlying such progress.
引文
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[15]Kémoun P,Gronthos S,Snead ML,et al.The role of cell surface markers and enamel matrix derivatives on human periodontal ligament mesenchymal progenitor responses in vitro[J].Biomaterials,2011,32(30):7375-7388.
[16]Cheng L,Lin ZK,Shu R,et al.Analogous effects of recombinant human full-length amelogenin expressed by Pichia pastoris yeast and enamel matrix derivative in vitro[J].Cell Prolif,2012,45(5):456-465.
[17]Yoshimi Y,Kunimatsu R,Hirose N,et al.Effects of C-terminal amelogenin peptide on proliferation of human cementoblast lineage cells[J].J Periodontol,2016,87(7):820-827.
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[20]李希庭,束蓉,宋忠臣,等.重组猪釉原蛋白对人牙龈上皮细胞生物学活性的影响[J].上海口腔医学,2012,21(3):257-261.Li XT,Shu R,Song ZC,et al.The effects of recombinant porcine amelogenin on human gingival epithelial cells[J].Shanghai J Stomatol,2012,21(3):257-261.
[21]Olivares-Navarrete R,Vesper K,Hyzy SL,et al.Role of the N-terminal peptide of amelogenin on osteoblastic differentiation of human mesenchymal stem cells[J].Eur Cell Mater,2014,28:1-10.
[22]Katayama N,Kato H,Taguchi Y,et al.The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells[J].Int J Mol Sci,2014,15(8):14026-14043.
[23]Wu SM,Chiu HC,Chin YT,et al.Effects of enamel matrix derivative on the proliferation and osteogenic differentiation of human gingival mesenchymal stem cells[J].Stem Cell Res Ther,2014,5(2):52.
[24]Kunimatsu R,Yoshimi Y,Hirose N,et al.The C-terminus of amelogenin enhances osteogenic differentiation of human cementoblast lineage cells[J].J Periodont Res,2017,52(2):218-224.
[25]Kitagawa M,Kitagawa S,Nagasaki A,et al.Synthetic ameloblastin peptide stimulates differentiation of human periodontal ligament cells[J].Arch Oral Biol,2011,56(4):374-379.
[26]Logan CY,Nusse R.The Wnt signaling pathway in development and disease[J].Annu Rev Cell Dev Biol,2004,20:781-810.
[27]Warotayanont R,Frenkel B,Snead ML,et al.Leucinerich amelogenin peptide induces osteogenesis by activation of the Wnt pathway[J].Biochem Biophys Res Commun,2009,387(3):558-563.
[28]Carinci F,Piattelli A,Guida L,et al.Effects of Emdogain on osteoblast gene expression[J].Oral Dis,2006,12(3):329-342.
[29]Amin HD,Olsen I,Knowles J,et al.A tyrosine-rich amelogenin peptide promotes neovasculogenesis in vitro and ex vivo[J].Acta Biomater,2014,10(5):1930-1939.
[2]Chen FM,Sun HH,Lu H,et al.Stem cell-delivery therapeutics for periodontal tissue regeneration[J].Biomaterials,2012,33(27):6320-6344.
[3]Bj?rklund A,Dunnett SB,Brundin P,et al.Neural transplantation for the treatment of Parkinson’s disease[J].Lancet Neurol,2003,2(7):437-445.
[4]Cortellini P,Paolo G,Prato P,et al.Long-term stability of clinical attachment following guided tissue regeneration and conventional therapy[J].J Clin Periodontol,1996,23(2):106-111.
[5]Hammarstr?m L,Heijl L,Gestrelius S.Periodontal regeneration in a buccal dehiscence model in monkeys after application of enamel matrix proteins[J].J Clin Periodontol,1997,24(9 Pt 2):669-677.
[6]McCulloch CA,Bordin S.Role of fibroblast subpopulations in periodontal physiology and pathology[J].J Periodont Res,1991,26(3 Pt 1):144-154.
[7]Somerman MJ,Archer SY,Imm GR,et al.A comparative study of human periodontal ligament cells and gingival fibroblasts in vitro[J].J Dent Res,1988,67(1):66-70.
[8]Li XT,Shu R,Liu DL,et al.Different effects of25-kDa amelogenin on the proliferation,attachment and migration of various periodontal cells[J].Biochem Biophys Res Commun,2010,394(3):581-586.
[9]Kasaj A,Willershausen B,Junker R,et al.Human periodontal ligament fibroblasts stimulated by nanocrystalline hydroxyapatite paste or enamel matrix derivative.An in vitro assessment of PDL attachment,migration,and proliferation[J].Clin Oral Investig,2012,16(3):745-754.
[10]Cortellini P,Tonetti MS.Focus on intrabony defects:guided tissue regeneration[J].Periodontol 2000,2000,22:104-132.
[11]Seo BM,Miura M,Gronthos S,et al.Investigation of multipotent postnatal stem cells from human periodontal ligament[J].Lancet,2004,364(9429):149-155.
[12]Toyoda K,Fukuda T,Sanui T,et al.Grp78 is critical for amelogenin-induced cell migration in a multipotent clonal human periodontal ligament cell line[J].JCell Physiol,2016,231(2):414-427.
[13]Fukuda T,Sanui T,Toyoda K,et al.Identification of novel amelogenin-binding proteins by proteomics analysis[J].PLoS One,2013,8(10):e78129.
[14]Bosshardt DD.Biological mediators and periodontal regeneration:a review of enamel matrix proteins at the cellular and molecular levels[J].J Clin Periodontol,2008,35(8 Suppl):87-105.
[15]Kémoun P,Gronthos S,Snead ML,et al.The role of cell surface markers and enamel matrix derivatives on human periodontal ligament mesenchymal progenitor responses in vitro[J].Biomaterials,2011,32(30):7375-7388.
[16]Cheng L,Lin ZK,Shu R,et al.Analogous effects of recombinant human full-length amelogenin expressed by Pichia pastoris yeast and enamel matrix derivative in vitro[J].Cell Prolif,2012,45(5):456-465.
[17]Yoshimi Y,Kunimatsu R,Hirose N,et al.Effects of C-terminal amelogenin peptide on proliferation of human cementoblast lineage cells[J].J Periodontol,2016,87(7):820-827.
[18]Seger R,Krebs EG.The MAPK signaling cascade[J].FASEB J,1995,9(9):726-735.
[19]Huang Y,Tanimoto K,Tanne Y,et al.Effects of human full-length amelogenin on the proliferation of human mesenchymal stem cells derived from bone marrow[J].Cell Tissue Res,2010,342(2):205-212.
[20]李希庭,束蓉,宋忠臣,等.重组猪釉原蛋白对人牙龈上皮细胞生物学活性的影响[J].上海口腔医学,2012,21(3):257-261.Li XT,Shu R,Song ZC,et al.The effects of recombinant porcine amelogenin on human gingival epithelial cells[J].Shanghai J Stomatol,2012,21(3):257-261.
[21]Olivares-Navarrete R,Vesper K,Hyzy SL,et al.Role of the N-terminal peptide of amelogenin on osteoblastic differentiation of human mesenchymal stem cells[J].Eur Cell Mater,2014,28:1-10.
[22]Katayama N,Kato H,Taguchi Y,et al.The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells[J].Int J Mol Sci,2014,15(8):14026-14043.
[23]Wu SM,Chiu HC,Chin YT,et al.Effects of enamel matrix derivative on the proliferation and osteogenic differentiation of human gingival mesenchymal stem cells[J].Stem Cell Res Ther,2014,5(2):52.
[24]Kunimatsu R,Yoshimi Y,Hirose N,et al.The C-terminus of amelogenin enhances osteogenic differentiation of human cementoblast lineage cells[J].J Periodont Res,2017,52(2):218-224.
[25]Kitagawa M,Kitagawa S,Nagasaki A,et al.Synthetic ameloblastin peptide stimulates differentiation of human periodontal ligament cells[J].Arch Oral Biol,2011,56(4):374-379.
[26]Logan CY,Nusse R.The Wnt signaling pathway in development and disease[J].Annu Rev Cell Dev Biol,2004,20:781-810.
[27]Warotayanont R,Frenkel B,Snead ML,et al.Leucinerich amelogenin peptide induces osteogenesis by activation of the Wnt pathway[J].Biochem Biophys Res Commun,2009,387(3):558-563.
[28]Carinci F,Piattelli A,Guida L,et al.Effects of Emdogain on osteoblast gene expression[J].Oral Dis,2006,12(3):329-342.
[29]Amin HD,Olsen I,Knowles J,et al.A tyrosine-rich amelogenin peptide promotes neovasculogenesis in vitro and ex vivo[J].Acta Biomater,2014,10(5):1930-1939.