利胆颗粒质量标准的研究
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摘要
目的建立利胆颗粒质量标准。方法薄层色谱法鉴别白芍和姜黄;高效液相色谱法检测橙皮苷含量,色谱柱为Shimadzu VP-ODS C_(18) (250 mm×4.6 mm,5μm);流动相:以乙腈为流动相A,0.2%磷酸溶液为流动相B,梯度洗脱;流速:1.0 ml/min;柱温:30℃;检测波长:283 nm。结果薄层鉴别的色谱斑点清晰,阴性对照无干扰;橙皮苷在19.98～199.8μg/ml范围内呈良好的线性关系(r=0.9999),平均回收率97.41%,RSD为1.696%。结论该测定方法简单可行,可用于利胆颗粒定性、定量检测,可用于利胆颗粒的质量控制。
Objective To establish the quality standard of Lidan Granules. Methods TLC identi?cation of Paeoniae radix alba and Curcumae longae rhizoma was performed. Hesperidin was determined by HPLC. The separation was performed on Shimadzu VP-ODS C_(18)(250 mm×4.6 mm, 5 μm) column. Gradient elution was performed, using acetonitrile as mobile phase A and 0.2 % phosphoric acid solution as mobile phase B. The ?ow rate was 1.0 ml/min and the column temperature was 30 ℃. The detection wavelength was set at 283 nm. Results The TLC spots were clear and the negative control had no interference. Hesperidin showed a good linear relationship in the range of 19.98-199.8 μg/ml(r=0.9999). The average recovery of hesperidin was 97.41 %, with RSD of 1.696 %. Conclusion The method is simple and available, and can be used for the quality control of Lidan Granules.
Objective To establish the quality standard of Lidan Granules. Methods TLC identi?cation of Paeoniae radix alba and Curcumae longae rhizoma was performed. Hesperidin was determined by HPLC. The separation was performed on Shimadzu VP-ODS C_(18)(250 mm×4.6 mm, 5 μm) column. Gradient elution was performed, using acetonitrile as mobile phase A and 0.2 % phosphoric acid solution as mobile phase B. The ?ow rate was 1.0 ml/min and the column temperature was 30 ℃. The detection wavelength was set at 283 nm. Results The TLC spots were clear and the negative control had no interference. Hesperidin showed a good linear relationship in the range of 19.98-199.8 μg/ml(r=0.9999). The average recovery of hesperidin was 97.41 %, with RSD of 1.696 %. Conclusion The method is simple and available, and can be used for the quality control of Lidan Granules.
引文
[1]金英善,陈曼丽,陶俊.芍药化学成分和药理作用研究进展[J].中国药理学与毒理学杂志, 2013, 27(4):745-749.
[2]赵鹏,蔡辉.姜黄素药理作用研究进展[J].中医药临床杂志,2012, 24(4):380-382.
[3]张冬松,高慧媛,吴立军.橙皮苷的药理活性研究进展[J].中国现代中药, 2006, 8(7):25-27.
[4]于定荣,张村,顾雪竹.去皮与不去皮白芍饮片的色谱鉴别及其芍药苷含量测定比较[J].中国实验方剂学杂志, 2013, 19(21):159-161.
[5]李清娟,容彦华,陈素锐,等.阴清肺颗粒中芍药苷与玄参的同板薄层色谱鉴别[J].中国药师, 2014, 17(2):335-336.
[6]王青虎,白玉霞,布仁图雅.薄层比色法测定沙日嘎-4汤中姜黄素的含量[J].中国实验方剂学杂志, 1998, 4(5):12-13.
[7]马宏伟,常建晖,李怀平,等.复方藤果痔疮栓质量标准研究[J].中成药,2014,36(9):1872-1876.
[8]王静,秦伟,张全明. TLC法测定散结乳癖贴膏中姜黄素的含量[J].中国药房, 2007, 18(18):1394-1395.
[9]王宁芳.橙皮苷的提取工艺研究[J].安徽农业科学, 2009, 37(8):3759.
[10]吴怀恩,谢臻,杨谭,等. RP-HPLC法测定醋青皮配方颗粒中橙皮苷的含量[J].中国实验方剂学杂志, 2010, 16(8):61-63.
[11]吴愫青,叶莹,张俊. HPLC测定不同产地陈皮橙皮苷的含量[J].中国现代中药, 2008, 10(8):20-22.
[12]蔡庆顺,钟小群,余华.陈皮不同提取工艺橙皮苷提取率分析[J].中成药, 2010, 3(26):1067-1070.
[13]胡志军,陈建秋.HPLC测定不同基原陈皮药材中橙皮苷含量[J].中国实验方剂学杂志,2012, 18(10):95-97.
[14]叶鹏.HPLC法测定健胃消食片中橙皮苷的含量[J].当代医学,2010, 16(26):154.
[2]赵鹏,蔡辉.姜黄素药理作用研究进展[J].中医药临床杂志,2012, 24(4):380-382.
[3]张冬松,高慧媛,吴立军.橙皮苷的药理活性研究进展[J].中国现代中药, 2006, 8(7):25-27.
[4]于定荣,张村,顾雪竹.去皮与不去皮白芍饮片的色谱鉴别及其芍药苷含量测定比较[J].中国实验方剂学杂志, 2013, 19(21):159-161.
[5]李清娟,容彦华,陈素锐,等.阴清肺颗粒中芍药苷与玄参的同板薄层色谱鉴别[J].中国药师, 2014, 17(2):335-336.
[6]王青虎,白玉霞,布仁图雅.薄层比色法测定沙日嘎-4汤中姜黄素的含量[J].中国实验方剂学杂志, 1998, 4(5):12-13.
[7]马宏伟,常建晖,李怀平,等.复方藤果痔疮栓质量标准研究[J].中成药,2014,36(9):1872-1876.
[8]王静,秦伟,张全明. TLC法测定散结乳癖贴膏中姜黄素的含量[J].中国药房, 2007, 18(18):1394-1395.
[9]王宁芳.橙皮苷的提取工艺研究[J].安徽农业科学, 2009, 37(8):3759.
[10]吴怀恩,谢臻,杨谭,等. RP-HPLC法测定醋青皮配方颗粒中橙皮苷的含量[J].中国实验方剂学杂志, 2010, 16(8):61-63.
[11]吴愫青,叶莹,张俊. HPLC测定不同产地陈皮橙皮苷的含量[J].中国现代中药, 2008, 10(8):20-22.
[12]蔡庆顺,钟小群,余华.陈皮不同提取工艺橙皮苷提取率分析[J].中成药, 2010, 3(26):1067-1070.
[13]胡志军,陈建秋.HPLC测定不同基原陈皮药材中橙皮苷含量[J].中国实验方剂学杂志,2012, 18(10):95-97.
[14]叶鹏.HPLC法测定健胃消食片中橙皮苷的含量[J].当代医学,2010, 16(26):154.