微曝气-ABR处理养猪废水及微生物群落分布
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摘要
本文研究微曝气-ABR处理养猪场废水的效能,同时对ABR中厌氧污泥的脱氢酶、蛋白酶活性以及微生物群落结构进行分析。在预处理微曝气时间8h,ABR水力停留时间24h,并在ABR第四格室与第一格室间增加回流的条件下,整体流程COD去除率为70%～90%,氨氮的去除率为65%～75%,其中ABR对COD的去除率为60%～80%,对氨氮的去除率为40%～55%。ABR中各个格室的脱氢酶、蛋白酶的含量逐渐降低,第一格室污泥中的脱氢酶与蛋白酶的含量分别为41.06mg/(g·h)和0.42mg/g。高通量测序表明,ABR反应器中绿弯菌门、壁菌门、变形菌门最为丰富,特别是绿弯菌门在4个格室中的比例分别为51.12%、46.96%、62.44%和47.23%,绿弯菌门的存在有利于复杂污染物的降解;同时由Heatmap图可知,由于回流的存在,使得第四格室与第一格室的微生物群落分布更为接近。
The efficiency of the microaeration-anaerobic baffled reactor(ABR)was investigated in treating swine wastewater.In addition,the activity of dehydrogenase,protease and micro-ecology in the ABR were also analyzed.At the operation conditions the pretreatment with 8 hours of micro aeration,ABR of the hydraulic retention time was 24 hours,and the adding reflux between the first compartment and the fourth compartment of the ABR,the removal efficiency of COD reached to 70%-90%in the total system,and the removal efficiency of COD was 60%-80%in the ABR.Meanwhile,the removal rate of NH3-N reached to 65%-75%,40%-55%,respectively in the total system and ABR.The content of dehydrogenase and protease in each compartment of ABR gradually decreased,and the content ofdehydrogenase and protease was 41.06 mg/(g·h)and 0.42 mg/g in the first compartment of the ABR,respectively.According to the high-throughput sequencing results, Chloroflexi, Firmicutes,Proteobacteria were dominant bacteria in the ABR sludge in particular,the abundance of Chloroflexi was 51.12%,46.96%,62.44% and 47.23% in each compartment.Chloroflexi is beneficial to the degradation of a series of complex pollutants.At the same time,known from the heatmap figure,the microbial community structures in the fourth compartment and the first compartment were much closer due to the existence of reflux.
The efficiency of the microaeration-anaerobic baffled reactor(ABR)was investigated in treating swine wastewater.In addition,the activity of dehydrogenase,protease and micro-ecology in the ABR were also analyzed.At the operation conditions the pretreatment with 8 hours of micro aeration,ABR of the hydraulic retention time was 24 hours,and the adding reflux between the first compartment and the fourth compartment of the ABR,the removal efficiency of COD reached to 70%-90%in the total system,and the removal efficiency of COD was 60%-80%in the ABR.Meanwhile,the removal rate of NH3-N reached to 65%-75%,40%-55%,respectively in the total system and ABR.The content of dehydrogenase and protease in each compartment of ABR gradually decreased,and the content ofdehydrogenase and protease was 41.06 mg/(g·h)and 0.42 mg/g in the first compartment of the ABR,respectively.According to the high-throughput sequencing results, Chloroflexi, Firmicutes,Proteobacteria were dominant bacteria in the ABR sludge in particular,the abundance of Chloroflexi was 51.12%,46.96%,62.44% and 47.23% in each compartment.Chloroflexi is beneficial to the degradation of a series of complex pollutants.At the same time,known from the heatmap figure,the microbial community structures in the fourth compartment and the first compartment were much closer due to the existence of reflux.
引文
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[16]闫媛,黎力,王亚宜,等.采用高通量测序分析全程自养脱(CANON)系统不同脱氮效能下的微生物群落结构[J].北京工业大学学报,2015,41(10):1489-1492.DOI:10.11936/bjutxb2015010005.
[2]钟成华,张文东,刘鹏,等.包埋固定化复合菌低温下处理养猪废水研究[J].广西师范大学学报(自然科学报),2011,29(3):52-56.DOI:10.16088/j.issn.1001-6600.2011.03.008.
[3]麻泽龙,周芸,王朝勇,等.养殖污水处理与高效再利用系统的设计[J].中国给水排水,2009,25(6):44-47.DOI:10.3321/j.issn:1000-4602.2009.06.011.
[4]于帅,李锦,毛大庆,等.抗生素抗性基因在废水处理系统的来源、传播扩散、归趋以及污染控制研究进展[J].环境化学,2013,32(11):2059-2071.DOI:10.7524/j.issn.0254-6108.2013.11.008.
[5] LU Xueqin,ZHEN Guangyin,NI Jialing,et al.Effect of influent COD/SO2-4ratios on biodegradation behaviors of starch wastewater in an upow anaerobic sludge blanket(UASB)reactor[J].Bioresource Technology,2016,214:175-183.DOI:10.1016/j.biortech.2016.04.100.
[6] CHEN Chongjun,SUN Faqian,ZHANG Haiqing,et al.Evaluation of COD effect on anammox process and microbial communities in the anaerobic bafed reactor(ABR)[J].Bioresource Technology,2016,216:571-578.DOI:10.1016/j.biortech.2016.05.115.
[7]詹旭,王翔,高金桃.采用ABR法处理养猪场污水[J].工业水处理,2013,33(2):80-82.DOI:10.3969/j.issn.1005-829X.2013.02.023.
[8]李刚,周兴求,伍健东.微氧条件下厌氧折流板反应器运行特性研究[J].环境工程学报,2009,3(7):1263-1267.
[9] CHEN Y,CHENG J J,CREAMER K S.Inhibition of anaerobic digestion process:a review[J].Bioresource Technology,2008,99:4044-4064.DOI:10.1016/j.biortech.2007.01.057.
[10]康福丽,朱国政,林钰,等.AMF对金橘苗根围土壤酶活性及植株生长的影响[J].广西师范大学学报(自然科学版),2017,35(1):104-122.10.16088/j.issn.1001-6600.2017.01.017.
[11]李今,吴振斌,贺锋.生物膜活性测定中TTC-脱氢酶活性测定法的改进[J].吉首大学学报(自然科学版),2005,26(1):37-39.DOI:10.3969/j.issn.1007-2985.2005.01.011.
[12] SU Chengyuan,LI Weiguang,CHEN Menglin,et al.Effect of iron-manganese-sepiolite as heterogeneous Fenton-like catalyst on the performance and microbial community of anaerobic granular sludge treatment system[J].Bioresource Technology,2016,200:1065-1072.DOI:10.1016/j.biortech.2015.10.088.
[13] PAN Jing,FEI Hexin,SONG Siyu,et al.Effects of intermittent aeration on pollutants removal in subsurface wastewater infiltration system[J].Bioresource Technology,2015,191:327-311.DOI:10.1016/j.biortech.2015.05.023.
[14] BADDAM R,REDDY G B,RACZKOWSKI C,et al.Activity of soil enzymes in constructed wetlands treated with swine wastewater[J].Ecological Engineering,2016,91:24-30.DOI:10.1016/j.ecoleng.2016.01.021.
[15]陈重军,张海芹,汪瑶琪,等.基于高通量测序的ABR厌氧氨氧化反应器各隔室细菌群落特征分析[J].环境科学,2016,37(7):2652-2657.DOI:10.13227/j.hjkx.2016.07.031.
[16]闫媛,黎力,王亚宜,等.采用高通量测序分析全程自养脱(CANON)系统不同脱氮效能下的微生物群落结构[J].北京工业大学学报,2015,41(10):1489-1492.DOI:10.11936/bjutxb2015010005.