2018年广东省米面制品、淀粉及其制品中椰毒假单胞菌酵米面亚种的调查分析
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摘要
目的调查分析2018年广东省米面制品、淀粉及其制品中椰毒假单胞菌酵米面亚种。方法在广东省中选取粉丝粉条、河粉米粉等米面制品、淀粉及其制品的企业、超市、农贸市场及餐饮单位为采样点,随机抽取1570份样品按照GB/T 4789.29-2003《食品卫生微生物学检验椰毒假单胞菌酵米面亚种检验》进行检验和VITEK2鉴定。结果 1570份样品中5份检出唐菖蒲伯克霍尔德菌,其中只有1份检出椰毒假单胞菌酵米面亚种,检出率为0.06%(1/1570)。结论米面制品、淀粉及其制品中检出唐菖蒲伯克霍尔德菌,表明广东省米面制品、淀粉及其制品中存在被椰毒假单胞菌酵米面亚种污染风险。为最大限度降低或消除风险隐患,相关部门应加强安全监管,保障人民群众身体健康和饮食安全。
Objective To investigate and analyze the Pseudomonas cocovenenans subsp. farinofermentans from rice flour products and starch and its products in Guangdong province in 2018. Methods A total of 1570 samples of rice flour products, starch and its products were sampled from enterprises, supermarkets, farmers' markets and catering sectors in Guangdong province, and these samples were detected by method of GB/T 4789.29-2003 Microbiological examination of food hygiene-Examination of Pseudomonas cocovenenans subsp. farinofermentans,finally identified by VITEK 2. Results Among the 1570 samples, Burkholderia gladioli was detected in 5 samples,among which only 1 sample was detected with Pseudomonas cocovenenans subsp. farinofermentans, with the detection rate of 0.06%(1/1570). Conclusion Burkholderia gladioli is detected in rice flour products, starch and its products, indicating that this kind of food in Guangdong province is probably to be contaminated by Pseudomonas cocovenenans subsp. farinofermentans. In order to minimize or eliminate risks and hidden dangers, relevant departments should strengthen safety supervision to ensure people's health and food safety.
Objective To investigate and analyze the Pseudomonas cocovenenans subsp. farinofermentans from rice flour products and starch and its products in Guangdong province in 2018. Methods A total of 1570 samples of rice flour products, starch and its products were sampled from enterprises, supermarkets, farmers' markets and catering sectors in Guangdong province, and these samples were detected by method of GB/T 4789.29-2003 Microbiological examination of food hygiene-Examination of Pseudomonas cocovenenans subsp. farinofermentans,finally identified by VITEK 2. Results Among the 1570 samples, Burkholderia gladioli was detected in 5 samples,among which only 1 sample was detected with Pseudomonas cocovenenans subsp. farinofermentans, with the detection rate of 0.06%(1/1570). Conclusion Burkholderia gladioli is detected in rice flour products, starch and its products, indicating that this kind of food in Guangdong province is probably to be contaminated by Pseudomonas cocovenenans subsp. farinofermentans. In order to minimize or eliminate risks and hidden dangers, relevant departments should strengthen safety supervision to ensure people's health and food safety.
引文
[1]刘秀梅.我国椰毒假单胞菌酵米面亚种食物中毒流行趋势浅析[J].中华预防医学杂志,1996,(6):54-56.Liu XM.Analysis on the epidemic trend of food poisoning of Pseudomonas cocovenenans subsp farinofermentans[J].Chin J Prev Med,1996,(6):54-56.
[2]孟昭赫,刘秀梅,王淑真,等.椰毒假单胞菌酵米面亚种食物中毒病原及预防的研究[J].医学研究通讯,1997,(12):10-11.Meng ZH,Liu XM,Wang SZ,et al.Study on the pathogenic factors and prevention of food poisoning caused by Pseudomonas co-covenena-ns subsp farinofermentans[J].J Med Res,1997,(12):10-11.
[3]陈瑾.椰毒假单胞菌中毒案例[J].法制博览,2016,(36):117.Chen J.Cases of Pseudomonas cocovenenans poisoning[J].Legal Vision,2016,(36):117.
[4]王静,刘秀梅.椰毒假单胞菌酵米面亚种及米酵菌酸的研究进展(综述)[J].中国食品卫生杂志,1996,(2):43-46,49.Wang J,Liu XM.Research progress on Pseudomonas cocovenenans subsp farinofermentans and bongkreic acid(review)[J].Chin J Food Hyg,1996,(2):43-46,49.
[5]沈莹,刘军,黄兆勇,等.1990~2006年广西酵米面食物中毒流行病学分析[J].中国热带医学,2007,(5):814-815.Shen Y,Liu J,Huang ZY,et al.Epidemiological analysis of food poisoning due to contamination of fermented flour with Pseud-omonas coc-ovenenans subsp,farinofermentans in Guangxi[J].China Tropical Med,2007,(5):814-815.
[6]刘志涛,万蓉,胡太芬,等.一起椰毒假单胞菌酵米面亚种引起的食物中毒调查分析[J].职业与健康,2013,29(5):582-583.Liu ZT,Wang R,Hu TF,et al.Investigation on a food poisoning incident induced by Pseudomonas cocovenenans subsp farinofe-rmentans[J].Occup Health,2013,29(5):582-583.
[7]周帼萍,梁泉,黄庭轩,等.云南省文山州广南县吊浆粑食物中毒事件的病原学分析[J].中国食品卫生杂志,2017,29(1):71-75.Zhou GP,Liang Q,Huang TX,et al.Etiology analysis of an outbreak caused by fermented corn flour in Guangnan county,Wenshan city,Yunnan province[J].Chin J Food Hyg,2017,29(1):71-75.
[8]李晓琍,杨祖顺,国译丹,等.椰毒假单胞菌酵米面亚种食物中毒的病原分离鉴定[J].中国食品卫生杂志,2016,28(1):36-39.Li XL,Yang ZS,Guo YD,et al.Isolation and identification of Pseudomonas ocovenenans subsp farinofermentans from food poisoning accident[J].Chin J Food Hyg,2016,28(1):36-39.
[9]郭学荣,李振营,程海鹰,等.椰毒假单胞菌食物中毒尸检3例[J].法医学杂志,2011,27(1):75-76.Guo XR,Li ZY,Cheng HY,et al.Autopsy of 3 cases of Pseudomonas cocovenenans food poisoning[J].J Foren Med,2011,27(1):75-76.
[10]GB/T 4789.29-2003食品卫生微生物学检验椰毒假单胞菌酵米面亚种检验[S].GB/T 4789.29-2003 Microbiological examination of food hygieneExamination of Pseudomonas cocovenenans subsp.Farinofermentans[S].
[11]GB 5009.189-2016食品安全国家标准食品中米酵菌酸的测定[S].GB 5009.189-2016 National food safety standard-Determination of bongkreic acid in foods[S].
[12]李金钟.洋葱伯克霍尔德菌复合体的研究进展[J].临床检验杂志,2008,(3):233-235.Li JZ.Research progress on Burkholderia cepacia complex[J].J Clin Lab Sci,2008,(3):233-235.
[13]赵梅,陈妮,陈如寿,等.海南类鼻疽临床流行病学研究报告[J].实用药物与临床,2016,19(7):901-904.Zhao M,Chen N,Chen RS,et al.Clinical epidemiologic research of melioidosis in Hainan[J].Pract Pharm Clin Remed,2016,19(7):901-904.
[14]焦振泉,曹玮,余东敏,等.椰酵假单胞菌与唐菖蒲伯克霍尔德菌16S~23S rRNA基因间区序列的比较研究[J].中国食品卫生杂志,2008,(3):197-203.Jiao ZQ,Cao W,Yu DM,et al.Study on comparison of 16S~23S rRNA gene ISR sequence of Pseudomonas cocovenenans Subsp Farinofermentans strains and Burkholderia gladioli strains[J].Chin J Food Hyg,2008,(3):197-203.
[15]Jiao ZQ,Yoshiaki K,Noriko M,et al.Need to differentiate lethal toxin-producing strains of Burkholderia gladioli,which cause severe food poisoning:description of B.gladioli pathovar cocovenenans and an emended description of B.gladioli[J].Microbiol Immunol,2003,47(12):915-925.
[16]黄庭轩,赵梦馨,周帼萍,等.利用recA基因序列分析鉴定云南酵米面中伯克霍尔德菌属分离株[J].中国食品卫生杂志,2017,29(5):526-533.Huang TX,Zhao MX,Zhou GP,et al.Identification of isolates in fermented corn flour food poisoning case in China by rec A sequence analysis[J].Chin J Food Hyg,2017,29(5):526-533.
[2]孟昭赫,刘秀梅,王淑真,等.椰毒假单胞菌酵米面亚种食物中毒病原及预防的研究[J].医学研究通讯,1997,(12):10-11.Meng ZH,Liu XM,Wang SZ,et al.Study on the pathogenic factors and prevention of food poisoning caused by Pseudomonas co-covenena-ns subsp farinofermentans[J].J Med Res,1997,(12):10-11.
[3]陈瑾.椰毒假单胞菌中毒案例[J].法制博览,2016,(36):117.Chen J.Cases of Pseudomonas cocovenenans poisoning[J].Legal Vision,2016,(36):117.
[4]王静,刘秀梅.椰毒假单胞菌酵米面亚种及米酵菌酸的研究进展(综述)[J].中国食品卫生杂志,1996,(2):43-46,49.Wang J,Liu XM.Research progress on Pseudomonas cocovenenans subsp farinofermentans and bongkreic acid(review)[J].Chin J Food Hyg,1996,(2):43-46,49.
[5]沈莹,刘军,黄兆勇,等.1990~2006年广西酵米面食物中毒流行病学分析[J].中国热带医学,2007,(5):814-815.Shen Y,Liu J,Huang ZY,et al.Epidemiological analysis of food poisoning due to contamination of fermented flour with Pseud-omonas coc-ovenenans subsp,farinofermentans in Guangxi[J].China Tropical Med,2007,(5):814-815.
[6]刘志涛,万蓉,胡太芬,等.一起椰毒假单胞菌酵米面亚种引起的食物中毒调查分析[J].职业与健康,2013,29(5):582-583.Liu ZT,Wang R,Hu TF,et al.Investigation on a food poisoning incident induced by Pseudomonas cocovenenans subsp farinofe-rmentans[J].Occup Health,2013,29(5):582-583.
[7]周帼萍,梁泉,黄庭轩,等.云南省文山州广南县吊浆粑食物中毒事件的病原学分析[J].中国食品卫生杂志,2017,29(1):71-75.Zhou GP,Liang Q,Huang TX,et al.Etiology analysis of an outbreak caused by fermented corn flour in Guangnan county,Wenshan city,Yunnan province[J].Chin J Food Hyg,2017,29(1):71-75.
[8]李晓琍,杨祖顺,国译丹,等.椰毒假单胞菌酵米面亚种食物中毒的病原分离鉴定[J].中国食品卫生杂志,2016,28(1):36-39.Li XL,Yang ZS,Guo YD,et al.Isolation and identification of Pseudomonas ocovenenans subsp farinofermentans from food poisoning accident[J].Chin J Food Hyg,2016,28(1):36-39.
[9]郭学荣,李振营,程海鹰,等.椰毒假单胞菌食物中毒尸检3例[J].法医学杂志,2011,27(1):75-76.Guo XR,Li ZY,Cheng HY,et al.Autopsy of 3 cases of Pseudomonas cocovenenans food poisoning[J].J Foren Med,2011,27(1):75-76.
[10]GB/T 4789.29-2003食品卫生微生物学检验椰毒假单胞菌酵米面亚种检验[S].GB/T 4789.29-2003 Microbiological examination of food hygieneExamination of Pseudomonas cocovenenans subsp.Farinofermentans[S].
[11]GB 5009.189-2016食品安全国家标准食品中米酵菌酸的测定[S].GB 5009.189-2016 National food safety standard-Determination of bongkreic acid in foods[S].
[12]李金钟.洋葱伯克霍尔德菌复合体的研究进展[J].临床检验杂志,2008,(3):233-235.Li JZ.Research progress on Burkholderia cepacia complex[J].J Clin Lab Sci,2008,(3):233-235.
[13]赵梅,陈妮,陈如寿,等.海南类鼻疽临床流行病学研究报告[J].实用药物与临床,2016,19(7):901-904.Zhao M,Chen N,Chen RS,et al.Clinical epidemiologic research of melioidosis in Hainan[J].Pract Pharm Clin Remed,2016,19(7):901-904.
[14]焦振泉,曹玮,余东敏,等.椰酵假单胞菌与唐菖蒲伯克霍尔德菌16S~23S rRNA基因间区序列的比较研究[J].中国食品卫生杂志,2008,(3):197-203.Jiao ZQ,Cao W,Yu DM,et al.Study on comparison of 16S~23S rRNA gene ISR sequence of Pseudomonas cocovenenans Subsp Farinofermentans strains and Burkholderia gladioli strains[J].Chin J Food Hyg,2008,(3):197-203.
[15]Jiao ZQ,Yoshiaki K,Noriko M,et al.Need to differentiate lethal toxin-producing strains of Burkholderia gladioli,which cause severe food poisoning:description of B.gladioli pathovar cocovenenans and an emended description of B.gladioli[J].Microbiol Immunol,2003,47(12):915-925.
[16]黄庭轩,赵梦馨,周帼萍,等.利用recA基因序列分析鉴定云南酵米面中伯克霍尔德菌属分离株[J].中国食品卫生杂志,2017,29(5):526-533.Huang TX,Zhao MX,Zhou GP,et al.Identification of isolates in fermented corn flour food poisoning case in China by rec A sequence analysis[J].Chin J Food Hyg,2017,29(5):526-533.