结球甘蓝高密度遗传图谱构建及抽薹时间相关QTL定位
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摘要
【目的】未熟抽薹是早春结球甘蓝产量损失的原因之一,为了进一步明确甘蓝抽薹性状的遗传基础.【方法】以易抽薹、晚熟、不结球的‘R_4P_1’为父本和晚抽薹、早熟、圆球的‘R_2P_2’为母本构建了126个重组自交系群体F7-8,从1 230个分子标记中筛选出408个多态性分子标记,并对控制甘蓝抽薹性状的QTL进行标记定位.【结果】387个分子标记定位到9个连锁群中,覆盖长度838cM,标记间平均图距2.2cM;在第8染色体上定位到1个与抽薹时间相关的QTL(qbt-2-2),其贡献率为9.1%,加性效应为-1.23,分子标记为CB10139.【结论】本研究构建的遗传图谱标记数量多,平均密度大,准确性高,标记CB10139可用于晚抽薹资源的筛选及耐抽薹甘蓝品种培育.
【Objective】Premature bolting is one of the reasons of yield decrease of heading cabbage in early spring,and the study was aimed to clarify the genetic basis of bolting character of cabbage.【Method】A mapping population consisting of 126 F7-8 recombinant inbred lines(RILs)derived from an intraspecific cross between R_4P_1(easy bolting,late-maturing and non-heading)and R_2P_2(late-bolting,early-maturing and heading)were used in this study.A total of 408 SSR polymorphic molecular markers were screened out from 1230 markers.A high-density linkage map was constructed by using Joinmap 4.0 and MapQTL 6.0,and the QTLs related to bolting character of cabbage were identified.【Result】Totally 387 markers located in 9 linkage groups were identified with 838 cM in length and an average distance of 2.2 cM between mark-ers.A QTL(qbt-2-2)located in chromosome 8 and associated with bolting time was identified,with the contribution rate of 9.1%and the additive effect of-1.23.Meanwhile,a molecular marker CB10139 with qbt-2-2 was also identified.【Conclusion】The genetic map constructed with a large number of markers was high marker density and high accuracy.The marker CB10139 can be used to screen late bolting cabbage resources for bolting-tolerant breeding.
【Objective】Premature bolting is one of the reasons of yield decrease of heading cabbage in early spring,and the study was aimed to clarify the genetic basis of bolting character of cabbage.【Method】A mapping population consisting of 126 F7-8 recombinant inbred lines(RILs)derived from an intraspecific cross between R_4P_1(easy bolting,late-maturing and non-heading)and R_2P_2(late-bolting,early-maturing and heading)were used in this study.A total of 408 SSR polymorphic molecular markers were screened out from 1230 markers.A high-density linkage map was constructed by using Joinmap 4.0 and MapQTL 6.0,and the QTLs related to bolting character of cabbage were identified.【Result】Totally 387 markers located in 9 linkage groups were identified with 838 cM in length and an average distance of 2.2 cM between mark-ers.A QTL(qbt-2-2)located in chromosome 8 and associated with bolting time was identified,with the contribution rate of 9.1%and the additive effect of-1.23.Meanwhile,a molecular marker CB10139 with qbt-2-2 was also identified.【Conclusion】The genetic map constructed with a large number of markers was high marker density and high accuracy.The marker CB10139 can be used to screen late bolting cabbage resources for bolting-tolerant breeding.
引文
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[2]陈毅勇,陈国平,王松,等.DNA分子标记在食用菌研究中的应用[J].现代化农业,2017(11):33-35.
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[6]陈书霞,王晓武,方智远,等.芥蓝×甘蓝F2群体抽薹期性状QTLs的RAPD标记[J].园艺学报,2003,30(4):421-426.
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[8]朱洪运,郁继华,简元才,等.结球甘蓝AFLP、SSR和SRAP标记高密度遗传图谱构建[J].华北农学报,2013,28(3):82-87.
[9]朱洪运,田多成,颉建明,等.结球甘蓝抽薹开花时间性状的QTL定位及分析[J].华北农学报,2013,28(5):1-5.
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[11]胡紫璟,毛娟,赵长增,等.利用SSR标记分析27份扁桃种质资源的亲缘关系[J].甘肃农业大学学报,2014,50(4):56-62.
[12]韦祖生,夏志强,李开绵,等.木薯种质遗传多样性的EST-SSE标记[J].热带作物学报,2008,29(3):304-309.
[13]陈琛,庄木,李康宁,等.甘蓝EST-SSR标记的开发与应用[J].园艺学报,2010,37(2):221-228.
[14]王庆彪,方志远,杨丽梅,等.中国50个甘蓝代表品种EST-SSR指纹图谱的构建系谱分析[J].中国农业科学,2014,47(1):111-121.
[15]苗明军,李成琼,司军,等.结球甘蓝西园4号种子纯度的SSR鉴定[J].中国蔬菜2011(2):53-55.
[16]袁天成,司军,宋洪元,等.结球甘蓝西园六号种子纯度的SSR和SRAP鉴定[J]西南大学学报2014,36(6).
[17]司军,李成琼,任雪松,等.结球甘蓝抗根肿病SSR分子连锁图谱的构建[J].植物病理学报,2011,41(3):305-309.
[18]宋立晓,严继勇,曾爱松,等.结球甘蓝遗传多样性的SSR分析[J].江苏农业学报,2013,29(1):151-156.
[19] Lv H H,Wang Q B,Zhang Y Y,et al.Linkage map construction using InDel and SSR markers and QTL analysis of heading traits in Brassica oleracea var.capitata L[J].Mol breeding.2014,34:87-98.
[20]田多成.结球甘蓝高密度分子遗传图谱的构建及硫代葡萄糖苷相关性状的QTL定位分析[D].兰州:甘肃农业大学,2014.
[21] Bohuon E J R,Ramsay L D,Craft J A,et al.The association of flowering time quantitative trait loci with duplicated regions and candidate loci in Brassica oleracea[J].Genetics,1998,150:393-401.
[22]赫荣楷,张建平,党占海,等.亚麻SRAP-PCR反应体系的优化建立及多态性标记筛选[J].甘肃农业大学学报,2013,49(5):43-49.
[23]朱洪运.结球甘蓝遗传图谱的构建及主要农艺性状的QTL定位[D].兰州:甘肃农业大学.
[24] Mccouch S R,Chen X,Panaud O,et al.Microsatellite marker development,mapping and applications in rice genetics and breeding[J].Plant Molbiol,1997,35:89-99.
[25]陈丽静,王利,王玉坤,等.利用重组自交系群体构建番茄AFLP遗传连锁图谱[J].园艺学报,2012,39(12):2377-2384.
[26]周斌,邢邯,陈受宜,等.大豆重组自交系群体NJRIKY遗传图谱的加密及其应用效果[J].作物学报,2010,36(1):36-46.
[27] Gao M,Li G,Yang B,et al.High-density Brassica oleracealinkage map identification of useful new linkages[J].TAG Theor Appl Genet,2007,115(2):277-287.
[28] Wang W X,Huang S M,LIU Y M,et al.Construction and analysis of a high-density genetic linkage map in cabbage(Brassica oleracea L.var.capitata)[J].BMC Genomics 2012,13:523.
[29]缪体云.结球甘蓝遗传图谱的构建及主要农艺性状的QTL定位[D].北京:中国农业科学院,2007.
[30]方宜钧,吴为人,唐纪良,等.作物DNA辅助育种[M].北京:科学出版社,2001.
[2]陈毅勇,陈国平,王松,等.DNA分子标记在食用菌研究中的应用[J].现代化农业,2017(11):33-35.
[3] Kennard W C,Slocum M K,Figdore S S,et al.Genetic analysis of morphological variation in Brassica oleracea using molecular markers[J].Theor Appl Genet,1994,87:721-732.
[4] Bohuon E J R,Ramsay L D,Craft J A,et al.The association of flowering time quantitative trait loci with duplicated regions and candidate loci in Brassica oleracea[J].Genetics,1998,150:393-401.
[5] Okazaki K,Sakamoto K,Kikuchi R,et al.Mapping and characterization of FLC homologs and QTL analysis of flowering time in Brassica oleracea[J].Theor Appl Genet,2007,114:595-608.
[6]陈书霞,王晓武,方智远,等.芥蓝×甘蓝F2群体抽薹期性状QTLs的RAPD标记[J].园艺学报,2003,30(4):421-426.
[7]李梅.结球甘蓝抽薹开花性状的遗传、QTL定位及生理研究[D].北京:中国农业科学院,2009.
[8]朱洪运,郁继华,简元才,等.结球甘蓝AFLP、SSR和SRAP标记高密度遗传图谱构建[J].华北农学报,2013,28(3):82-87.
[9]朱洪运,田多成,颉建明,等.结球甘蓝抽薹开花时间性状的QTL定位及分析[J].华北农学报,2013,28(5):1-5.
[10] Powell W,Machray G C,Provan J.Polymorphism revealed by simple sequence repeats[J].Trends Plant Science 1996,7:215-222.
[11]胡紫璟,毛娟,赵长增,等.利用SSR标记分析27份扁桃种质资源的亲缘关系[J].甘肃农业大学学报,2014,50(4):56-62.
[12]韦祖生,夏志强,李开绵,等.木薯种质遗传多样性的EST-SSE标记[J].热带作物学报,2008,29(3):304-309.
[13]陈琛,庄木,李康宁,等.甘蓝EST-SSR标记的开发与应用[J].园艺学报,2010,37(2):221-228.
[14]王庆彪,方志远,杨丽梅,等.中国50个甘蓝代表品种EST-SSR指纹图谱的构建系谱分析[J].中国农业科学,2014,47(1):111-121.
[15]苗明军,李成琼,司军,等.结球甘蓝西园4号种子纯度的SSR鉴定[J].中国蔬菜2011(2):53-55.
[16]袁天成,司军,宋洪元,等.结球甘蓝西园六号种子纯度的SSR和SRAP鉴定[J]西南大学学报2014,36(6).
[17]司军,李成琼,任雪松,等.结球甘蓝抗根肿病SSR分子连锁图谱的构建[J].植物病理学报,2011,41(3):305-309.
[18]宋立晓,严继勇,曾爱松,等.结球甘蓝遗传多样性的SSR分析[J].江苏农业学报,2013,29(1):151-156.
[19] Lv H H,Wang Q B,Zhang Y Y,et al.Linkage map construction using InDel and SSR markers and QTL analysis of heading traits in Brassica oleracea var.capitata L[J].Mol breeding.2014,34:87-98.
[20]田多成.结球甘蓝高密度分子遗传图谱的构建及硫代葡萄糖苷相关性状的QTL定位分析[D].兰州:甘肃农业大学,2014.
[21] Bohuon E J R,Ramsay L D,Craft J A,et al.The association of flowering time quantitative trait loci with duplicated regions and candidate loci in Brassica oleracea[J].Genetics,1998,150:393-401.
[22]赫荣楷,张建平,党占海,等.亚麻SRAP-PCR反应体系的优化建立及多态性标记筛选[J].甘肃农业大学学报,2013,49(5):43-49.
[23]朱洪运.结球甘蓝遗传图谱的构建及主要农艺性状的QTL定位[D].兰州:甘肃农业大学.
[24] Mccouch S R,Chen X,Panaud O,et al.Microsatellite marker development,mapping and applications in rice genetics and breeding[J].Plant Molbiol,1997,35:89-99.
[25]陈丽静,王利,王玉坤,等.利用重组自交系群体构建番茄AFLP遗传连锁图谱[J].园艺学报,2012,39(12):2377-2384.
[26]周斌,邢邯,陈受宜,等.大豆重组自交系群体NJRIKY遗传图谱的加密及其应用效果[J].作物学报,2010,36(1):36-46.
[27] Gao M,Li G,Yang B,et al.High-density Brassica oleracealinkage map identification of useful new linkages[J].TAG Theor Appl Genet,2007,115(2):277-287.
[28] Wang W X,Huang S M,LIU Y M,et al.Construction and analysis of a high-density genetic linkage map in cabbage(Brassica oleracea L.var.capitata)[J].BMC Genomics 2012,13:523.
[29]缪体云.结球甘蓝遗传图谱的构建及主要农艺性状的QTL定位[D].北京:中国农业科学院,2007.
[30]方宜钧,吴为人,唐纪良,等.作物DNA辅助育种[M].北京:科学出版社,2001.